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Citations for R&D Systems :
51 - 100 of 2125 citations for TIM 4 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 4 ng/ml human transforming growth factor α (TGFα; R&D Systems, MN, USA) as a positive control ...
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bioRxiv - Genomics 2021Quote: ... Polyclonal Goat Anti- Human/Mouse Oct-3/4 Antibody (R&D System, AF1759,1:20).
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bioRxiv - Bioengineering 2023Quote: ... 10 ng mL− 1 BMP-4 (human recombinant) (R&D Systems, Minneapolis, MN, USA), 10 µM LY294002 (Cayman Chemical ...
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bioRxiv - Immunology 2024Quote: ... and 10ng/mL of recombinant human IL-4 (R&D systems, #204-IL/CF). Inhibitors used were the PI3Kdelta inhibitor Idelalisib ...
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bioRxiv - Bioengineering 2023Quote: ... vascular endothelial growth factor (VEGF) (R&D Systems, #293-FB/CF), epidermal growth factor (EGF ...
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bioRxiv - Immunology 2021Quote: ... hBTLA-6×His tag (R&D systems) and hLIGHT-6×His tag proteins (made by the methods described above ...
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bioRxiv - Biophysics 2024Quote: ... Anti-HIS antibody (R&D systems, BAM050) coated polystyrene beads were prepared as previously described.39 The beads were decorated with native purified Dsn1-6HIS-3Flag kinetochores ...
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bioRxiv - Molecular Biology 2022Quote: Human recombinant BMP-2 and BMP-4 were purchase from R&D Systems (Minneapolis, MN). Anti-Myc and anti-HA antibodies were purchased from Cell Signaling Technology (Danvers ...
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bioRxiv - Bioengineering 2023Quote: ... and VEGF 165 (293-VE) were all purchased from R&D Systems Inc ...
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bioRxiv - Immunology 2021Quote: ... 109-006-127), 250ng/ml human recombinant MEGACD40L (Enzo, ALX-522-110) and 20ng/ml Recombinant human IL-4 (R&D system, 204-IL-010). Cells were collected at specific time points for analysis.
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bioRxiv - Immunology 2021Quote: ... were coated with recombinant human CTLA-4-Fc (7268-CT, R&D Systems, Minneapolis, MN, USA) at 1 μg/mL overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Human VEGFR3/Flt-4 antibody (1:500, AF743) was obtained from R&D systems (Minneapolis, MN). Horse Radish Peroxidase (HRP ...
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bioRxiv - Physiology 2024Quote: ... then stimulated with recombinant human IL-4 at 50 ng/mL (R&D Systems, Minneapolis, MN) in BEBM medium for 6 h or BEBM medium as control condition ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse anti-His primary antibody (R&D systems) was used at 1:1000 dilution in TBS ...
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bioRxiv - Biochemistry 2020Quote: ... His-ubiquitin (R&D Systems; Cat. # U-530), HA-ubiquitin (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... His-ubiquitin (R&D Systems; Cat. # U-530), HA-ubiquitin (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... His-HBP was purchased from R&D Systems. Purified His-HBP was incubated with beads bound with GST (control) ...
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bioRxiv - Cell Biology 2023Quote: ... with 10% HI-FBS (R&D Systems S11150), 100x Pen/Strep (Gibco 15140-122) ...
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bioRxiv - Cell Biology 2022Quote: ... and stained overnight at 4°C with goat anti-human SPP1 antibody (R&D Systems, AF1433-SP), followed by secondary staining with donkey anti-goat AF594 (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were labeled with anti-human SSEA-4 APC-conjugated antibody (1:100, R&D Systems) at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant human IL-4 500 IU/mL and GM- CSF 800 IU/mL (both R&D systems) at a cell density of 1-2 x106 cells/mL for 5 days ...
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bioRxiv - Neuroscience 2022Quote: ... The pre-immobilized beads were then incubated with 50 nM recombinant mouse SorCS1 ectodomain tagged with a C-terminal 6-His tag (SorCS1-His, Cat# 4395-SR-050, R&D systems) in ECS for 2 h at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... and His-p53 was obtained from R&D system.
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bioRxiv - Neuroscience 2023Quote: ... Either 1µL 500nM recombinant His-USP7 (R&D system) or 1µL deubiquitination buffer as vehicle control was added to each reaction and incubated for 1 hour at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... hiPSCs were incubated with the primary antibody goat anti-human OCT3/4 (R&D Systems, Minneapolis, MN, USA), mouse anti-human SOX2 (R&D Systems) ...
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bioRxiv - Immunology 2022Quote: ... were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody (R&D Systems, # MAB306). The next day ...
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bioRxiv - Bioengineering 2023Quote: ... medium was changed every day to HDM with 20 ng/mL human recombinant BMP-4 (R&D Systems) and 20 ng/mL human recombinant FGF-4 (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... the same protein was labeled using its His-tag by incubating the antigen with an Alexa Fluor 647-conjugated anti-His-antibody (R&D Systems, IC0501R) for 30 minutes at room temperature at a 2:1 ratio (RBD molecules:IgG molecules) ...
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bioRxiv - Biochemistry 2020Quote: ... His-MBP-PINK1 (R&D Systems, Cat. # AP-180-100) concentration was set to 50 ng/µl ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant His-tagged NSP12 (R&D Systems, catalog # 10686-CV) was incubated with in vitro transcribed and biotinylated RNA in 20 mM Tris ...
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bioRxiv - Bioengineering 2024Quote: ... His-tagged mouse TfR (TfR cross-reactivity, R&D Systems 9706-TR-050 ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the tissue sections were incubated overnight at 4°C with antibodies against human DDR1 (R&D Systems, AF2396), CXCL5 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human galectin-4 (GAL4 P56470) and gelsolin (GSN Q3SX14) from bovine plasma were purchased from R&D Systems and Sigma respectively.
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bioRxiv - Biophysics 2019Quote: ... Ibidi channels IV0.4 (Clinisciences) were coated overnight at 4°C with 10μg/ml human ICAM-l-Fc (R&D Systems) in Phosphate Buffer Solution (PBS ...
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bioRxiv - Immunology 2021Quote: ... biotinylated ACE2-hFc or mouse anti-His mAb (R&D systems). Spike trimer (1 µM binding sites ...
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bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
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bioRxiv - Cell Biology 2020Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA).
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bioRxiv - Cell Biology 2021Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA). MCF7 ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK 293T cells were seeded on plates coated with Cultrex Poly-L-Lysine (R&D Systems) prior to transfection with the pCCl-c-MNDU3 STEAP1-BBζ CAR lentiviral plasmid and the packaging plasmids pMDL ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... 10 μg/mL anti-His tag antibody (clone AD1.1.10, R&D Systems), 50 ng/mL CpG ODN 2006 (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... 10 µg/mL anti-His tag antibody (clone AD1.1.10, R&D Systems), 50 ng/mL CpG ODN 2006 (Invivogen) ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged ALS protein (R&D Systems, 10 ng/well, 1.5 µM) was immobilized in wells via neutravidin and the nickel-charged-NTA-decorated iBodies® (IOCB Tech ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Immunology 2022Quote: The supernatants collected from LPS-stimulated moDCs with or without fhFABP1 (25 μg/ml) were pooled from 4 donors in equal volume and subjected to the Proteome Profiler Human XL Cytokine Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... non-tissue culture plates were coated overnight at 4゜C with recombinant human B7-H3 protein (R&D Systems, Minneapolis, MN) at 10 μg/well in 500 μL of PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Resting or ADP (10 μM)-stimulated platelets (4 × 103/μL) were pretreated with the inhibitor hDcR3 (10 μg/mL, recombinant human decoy receptor 3 protein, R&D Systems) for 10 min and then allowed to adhere to the coated coverslips for 60 min at RT under normoxic or hypoxic conditions ...
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bioRxiv - Microbiology 2019Quote: ... Primary keratinocytes or HEK 293T cells were labelled with nectin-4PE antibody (clone 337516; R&D Systems) to assess the expression of nectin-4 ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with 100 ng Tie2‐Fc (with His tag, R&D systems) in TBST buffer at 4°C for 4 hours ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of isolated EV sample was mixed with 4 μL of Alexa488 anti-human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN) and 2 μL of PE anti-human CD63 antibody (561925 ...