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Citations for R&D Systems :
151 - 200 of 282 citations for Strong Cation Exchange Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: IL-1α or IL-1β concentrations in conditioned media were determined using the commercial DuoSet ELISA Kit (R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2024Quote: ... Cells were treated with fresh A549 media with or without human IFN-β recombinant protein (R&D Systems™ 8499IF010) at the indicated concentrations ...
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bioRxiv - Immunology 2024Quote: ... Iron free media was supplemented with set concentrations of human holotransferrin (R&D systems, 2914-HT-001G/Sigma Aldrich, T0665) ranging from 0.0002-0.625 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Wells were flooded with BrainPhys media with supplements and 10 ng/mL human NGF (256-GF-100, R&D Systems), and 3 μg/mL FRDU and incubated at 37° C and 5% CO2 until further experimentation.
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bioRxiv - Immunology 2024Quote: ... and were kept overnight in complete RPMI media with 50 ng/ml of recombinant human IL-2 (R&D Systems). NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend ...
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bioRxiv - Genetics 2019Quote: ... counted and seeded at 5.5 × 106 cells per well in 6-well suspension plates in 5.5 ml hESC media supplemented with 10 ng /ml activin A (R&D Systems) and 10 ng/ ml heregulinB (Peprotech) ...
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bioRxiv - Microbiology 2019Quote: ... was added to 2mL of fresh differentiation media and either 50 ng/µl of human IFNγ 285-IF (R&D systems) was added or not ...
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bioRxiv - Biochemistry 2021Quote: ... the media were replaced with serum-free medium containing 10 μg/ml normal goat IgG or LDLR Ab (R&D Systems) in the continued presence of 0 or 100 ng/ml TNFα for 0 ...
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bioRxiv - Cell Biology 2020Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA).
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bioRxiv - Microbiology 2020Quote: ... the endodermal cells were dissociated into single cells using Accutase and plated on the Matrigel-coated plates in serum-free differentiation media (SFM) supplemented with 1.5 μM Dorsomorphin dihydrochloride (R&D System) and 10 μM SB431542 for 24h ...
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bioRxiv - Immunology 2020Quote: Primary murine T cells were grown in R10 media - RPMI plus 10% fetal bovine serum (FBS; R&D Systems, Minneapolis, MN) supplemented with GlutaMAX (Gibco ...
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bioRxiv - Immunology 2019Quote: ... and grown in B cell growth media to 80% confluence before being treated for 4h with 5μg/ml mitomycin C (Tocris, R&D Systems). Monolayers of growth arrested cells were washed 3 times with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA). MCF7 ...
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bioRxiv - Cancer Biology 2020Quote: ... media was replaced with complete αMEM treated with 25 ng/mL murine MCSF and 30 ng/mL RANKL (R&D Systems) to induce osteoclastogenesis ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated in HITES media in the following conditions: 10 μg/mL Fibronectin (Cat no. 1918-FN-02M, R&D Systems) coated ...
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bioRxiv - Developmental Biology 2023Quote: ... NSPC culture in adherent and non-adherent proliferating conditions using media supplemented with human recombinant EGF and FGF2 (R&D Systems) was performed according to published protocols (Soldati et al ...
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bioRxiv - Molecular Biology 2022Quote: ... 450 μl of cell culture media was treated and incubated with the Proteome profiler mouse cytokine array membrane (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... into 40 ml of R10 media supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050). Cells were passaged twice per week and feeder cells were added in a 1:1 ratio after 7 days to maintain stimulation ...
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bioRxiv - Immunology 2023Quote: ... K562 cells were stimulated in complete RPMI media with 20 ng/mL human IFN-ψ (285-IF-100, R&D Systems), 20 ng/mL human IFN-β (84-99I-F010 ...
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bioRxiv - Immunology 2023Quote: ... HUT78 cells were stimulated in complete IMDM media with 20 ng/mL human IFN-ψ (285-IF-100, R&D Systems). All treatments were 24 hours.
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bioRxiv - Bioengineering 2023Quote: ... IFN-γ levels were measured in NK cell media samples using the human IFN-γ DuoSet ELISA kit (R&D Systems). IL-6 levels were measured in B cell media samples using the human IL-6 DuoSet ELISA kit (R&D Systems ...
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bioRxiv - Bioengineering 2023Quote: ... IL-6 levels were measured in B cell media samples using the human IL-6 DuoSet ELISA kit (R&D Systems) (53 ...
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bioRxiv - Bioengineering 2023Quote: OCI-AML5 cells were cultured in 24-well plates (3×105 cells/well) in cRPMI media (FBS from R&D Systems; Minneapolis ...
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bioRxiv - Molecular Biology 2023Quote: ... Colony forming assays were performed by plating 200 cells of each isolated subpopulation in Complete Methylcellulose Media (R&D Systems; HSC003).
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bioRxiv - Cell Biology 2023Quote: Isolated CD8+ DCs were cultured in R10 media supplemented with recombinant mouse FLT3L (250ng/ml; R&D Systems, 427-FL-025). CD8+ DCs isolated from Rubicon-proficient mice were incubated in the presence or absence of 5μM Fendiline-HCl (Tocris ...
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bioRxiv - Cell Biology 2024Quote: The levels of CXCL9 secreted to the media by MIN6 cells were quantified by Mouse DuoSet ELISA kit (R&D Systems). Polystyrene 96-well plates were coated with 100 μL of 200 ng/mL unconjugated goat anti-mouse CXCL9 antibody in PBS at room temperature overnight ...
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bioRxiv - Bioengineering 2021Quote: ... basal media samples were collected for quantification of soluble CCSP via a premade Magnetic Luminex Performance Assay kit (Uteroglobin/SCGB1A1, R&D Systems). Culture media was collected from the bottom chamber of each tissue device and immediately frozen at −20°C until use ...
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bioRxiv - Bioengineering 2019Quote: ... 2013] consisting of the osteogenic media supplemented with 100 ng/mL BMP-2 (recombinant human BMP-2, 355-BM-050, R&D Systems). Media was replaced every 3 days ...
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bioRxiv - Immunology 2019Quote: ... Autologous NK cells were isolated by negative selection using a human NK Cell Isolation Kit (Miltenyi) and cultured in complete RPMI-1640 media with 300 IU/mL of IL-2 (R&D Systems) for 22 hours.
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bioRxiv - Cancer Biology 2019Quote: ... media was removed from astrocytes and levels of cytokines were measured using the Rat XL Cytokine Array Kit (R&D Systems ARY030) using 1 mL of conditioned media per membrane ...
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bioRxiv - Bioengineering 2020Quote: ... Media for M(IFN-γ) stimulation consisted of RPMI 1640 supplemented with 10 ng/ml interferon-γ (IFN-γ; R&D systems), and media for M(IL4/IL13 ...
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bioRxiv - Cancer Biology 2020Quote: ... Presence of secreted IL-33 in the conditioned media was visualized on a Proteome Profiler Mouse XL Cytokine Array (R&D Systems). The concentration of IL-33 secreted in the conditioned medium was measured on a V-PLEX Mouse Cytokine 19-Plex Kit (Meso Scale Diagnostics ...
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bioRxiv - Cell Biology 2021Quote: ... PDGFRα homodimer cells and PDGFRβ homodimer cells at ∼60-70% confluence were serum starved for 24 h in HITES media and stimulated with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... and inserts were immersed in 500 µL RPMI media containing 10% FBS in the absence or presence of 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) for 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... A feeding layer of 2 mL RPMI growth media supplemented with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) was added on top of the agar and replaced every day ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were incubated overnight in myogenic growth media (F10, 20% FBS, 1% Pen/Strep; Gibco, supplemented with 5 ng/mL rFGF2; R&D Systems).
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bioRxiv - Molecular Biology 2022Quote: ... The levels of IL11 in equal volumes of cell culture media were quantified using Human IL11 Quantikine ELISA kit (D1100; R&D Systems). Both assays were performed according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... monolayers grown with the differentiation media for 7 days were stimulated with human IFNβ (100 or 300 IU/ml, R&D systems) or IFNλ2 (10 or 30 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in the media (RPMI1640 +2% FBS) with human anti-Nectin-2 monoclonal antibody or isotype control (20 μg/mL, both R&D Systems) for 6h ...
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bioRxiv - Developmental Biology 2020Quote: All OPCs were grown on PO and laminin coated flasks in growth media consisting of DMEM/F12 supplemented with N2 Max (R&D Systems), B27 (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: SUM159-LM1 cells or MDA231-LM2 cells were suspended in HuMEC/0.1% BSA and treated with EC-derived conditioned media (CM) (diluted 2-5 times in the final medium) or 50 ng/ml of recombinant activin B (R&D systems). 2,500 cells/well of SUM159-LM1 cells or 5,000 cells/well of MDA231-LM2 cells were seeded on 96-well ultra-low attachment plate (Corning) ...
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bioRxiv - Immunology 2020Quote: ... and then re-suspended in 30 μL of media containing 10,000 units/mL of recombinant IFN-α2a or 100 units/mL of recombinant IFN-γ (R&D Systems). Antibodies against SiglecF ...
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bioRxiv - Bioengineering 2019Quote: ... To induce differentiation FGF2 was withdrawn from the cell culture media and then replaced with bone morphogenic protein (BMP4; R&D Systems) or and Wnt3a (R&D Systems) ...
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bioRxiv - Genetics 2020Quote: ... femurs and tibias were harvested and bone-marrow cells were obtained by flushing bones and differentiated for 7 days in DMEM media supplemented with 50 mg ml-1 recombinant macrophage-colony stimulating factor (M-CSF, R&D systems), 20% heat-inactivated FBS ...
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bioRxiv - Cell Biology 2021Quote: Cytokine array was carried out in the young and aged L-MSC culture media using Proteome Profiler Mouse XL Cytokine Array kit (R&D Systems) as per manufacturer ‘s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... and further differentiated into day 3 posterior foregut through the addition of CDM3 base media supplemented with FGF2 (20 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... and then definitive ectoderm was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with BMP4 (30 ng/mL; R&D Systems), PD0325901 (100 nM ...
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bioRxiv - Genomics 2022Quote: Three million cells from each triplicate were seeded separately in 6 well-plates in fresh RPMI media and left unstimulated or stimulated with universal interferon alpha (200U/ml) (R&D systems) for 24 hours ...