Labshake search
Citations for R&D Systems :
351 - 400 of 1197 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... counted and seeded at 5.5 × 106 cells per well in 6-well suspension plates in 5.5 ml hESC media supplemented with 10 ng /ml activin A (R&D Systems) and 10 ng/ ml heregulinB (Peprotech) ...
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bioRxiv - Microbiology 2019Quote: ... was added to 2mL of fresh differentiation media and either 50 ng/µl of human IFNγ 285-IF (R&D systems) was added or not ...
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bioRxiv - Biochemistry 2021Quote: ... the media were replaced with serum-free medium containing 10 μg/ml normal goat IgG or LDLR Ab (R&D Systems) in the continued presence of 0 or 100 ng/ml TNFα for 0 ...
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bioRxiv - Cell Biology 2020Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA).
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bioRxiv - Microbiology 2020Quote: ... the endodermal cells were dissociated into single cells using Accutase and plated on the Matrigel-coated plates in serum-free differentiation media (SFM) supplemented with 1.5 μM Dorsomorphin dihydrochloride (R&D System) and 10 μM SB431542 for 24h ...
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bioRxiv - Cell Biology 2021Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA). MCF7 ...
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bioRxiv - Cancer Biology 2020Quote: ... media was replaced with complete αMEM treated with 25 ng/mL murine MCSF and 30 ng/mL RANKL (R&D Systems) to induce osteoclastogenesis ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated in HITES media in the following conditions: 10 μg/mL Fibronectin (Cat no. 1918-FN-02M, R&D Systems) coated ...
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bioRxiv - Cancer Biology 2023Quote: ... into 40 ml of R10 media supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050). Cells were passaged twice per week and feeder cells were added in a 1:1 ratio after 7 days to maintain stimulation ...
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bioRxiv - Cell Biology 2023Quote: Isolated CD8+ DCs were cultured in R10 media supplemented with recombinant mouse FLT3L (250ng/ml; R&D Systems, 427-FL-025). CD8+ DCs isolated from Rubicon-proficient mice were incubated in the presence or absence of 5μM Fendiline-HCl (Tocris ...
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bioRxiv - Immunology 2022Quote: ... followed by 24-hour incubation in culture medium supplemented with 50 ng/ml IL10 (R&D System, Minneapolis, MN, USA). The resulting MDMs were then detached for stimulation using TrypLE Select (Gibco ...
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bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...
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bioRxiv - Cell Biology 2019Quote: Cytokine (IL6 and CXCL8) levels in tissue culture supernatants were determined by ELISA assay (DY206 and DY208; R&D Systems). All assays were performed according to the manufacturer’s instructions and read on a CLARIOstar microplate reader (BMG Labtech) ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from twice passaged nutrient broth cultures and incubated with 3 uM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Cancer Biology 2023Quote: The levels of INFγ were evaluated in supernatants obtained from 0.625:1 and 1.25:1 effector:target ratio overnight co-cultures of CAR T-cells:CAFs using the Human IFN-Gamma Quantikine Kit (R&D Systems) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... VEGF or PEDF secretion level in culture medium was measured using the VEGF ELISA kit (Invitrogen or R&D systems) and PEDF ELISA kit (R&D systems ...
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bioRxiv - Cell Biology 2023Quote: ... IFN-β amounts in the culture supernatant were measured by the human IFN-β ELISA kit (R&D Systems, DIFNB0) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2021Quote: ... basal media samples were collected for quantification of soluble CCSP via a premade Magnetic Luminex Performance Assay kit (Uteroglobin/SCGB1A1, R&D Systems). Culture media was collected from the bottom chamber of each tissue device and immediately frozen at −20°C until use ...
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bioRxiv - Bioengineering 2019Quote: ... 2013] consisting of the osteogenic media supplemented with 100 ng/mL BMP-2 (recombinant human BMP-2, 355-BM-050, R&D Systems). Media was replaced every 3 days ...
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bioRxiv - Cancer Biology 2019Quote: ... media was removed from astrocytes and levels of cytokines were measured using the Rat XL Cytokine Array Kit (R&D Systems ARY030) using 1 mL of conditioned media per membrane ...
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bioRxiv - Bioengineering 2020Quote: ... Media for M(IFN-γ) stimulation consisted of RPMI 1640 supplemented with 10 ng/ml interferon-γ (IFN-γ; R&D systems), and media for M(IL4/IL13 ...
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bioRxiv - Cancer Biology 2020Quote: ... Presence of secreted IL-33 in the conditioned media was visualized on a Proteome Profiler Mouse XL Cytokine Array (R&D Systems). The concentration of IL-33 secreted in the conditioned medium was measured on a V-PLEX Mouse Cytokine 19-Plex Kit (Meso Scale Diagnostics ...
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bioRxiv - Cell Biology 2021Quote: ... PDGFRα homodimer cells and PDGFRβ homodimer cells at ∼60-70% confluence were serum starved for 24 h in HITES media and stimulated with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... and inserts were immersed in 500 µL RPMI media containing 10% FBS in the absence or presence of 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) for 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... A feeding layer of 2 mL RPMI growth media supplemented with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) was added on top of the agar and replaced every day ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were incubated overnight in myogenic growth media (F10, 20% FBS, 1% Pen/Strep; Gibco, supplemented with 5 ng/mL rFGF2; R&D Systems).
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bioRxiv - Cell Biology 2022Quote: ... monolayers grown with the differentiation media for 7 days were stimulated with human IFNβ (100 or 300 IU/ml, R&D systems) or IFNλ2 (10 or 30 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in the media (RPMI1640 +2% FBS) with human anti-Nectin-2 monoclonal antibody or isotype control (20 μg/mL, both R&D Systems) for 6h ...
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bioRxiv - Developmental Biology 2020Quote: All OPCs were grown on PO and laminin coated flasks in growth media consisting of DMEM/F12 supplemented with N2 Max (R&D Systems), B27 (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: SUM159-LM1 cells or MDA231-LM2 cells were suspended in HuMEC/0.1% BSA and treated with EC-derived conditioned media (CM) (diluted 2-5 times in the final medium) or 50 ng/ml of recombinant activin B (R&D systems). 2,500 cells/well of SUM159-LM1 cells or 5,000 cells/well of MDA231-LM2 cells were seeded on 96-well ultra-low attachment plate (Corning) ...
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bioRxiv - Immunology 2020Quote: ... and then re-suspended in 30 μL of media containing 10,000 units/mL of recombinant IFN-α2a or 100 units/mL of recombinant IFN-γ (R&D Systems). Antibodies against SiglecF ...
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bioRxiv - Genetics 2020Quote: ... femurs and tibias were harvested and bone-marrow cells were obtained by flushing bones and differentiated for 7 days in DMEM media supplemented with 50 mg ml-1 recombinant macrophage-colony stimulating factor (M-CSF, R&D systems), 20% heat-inactivated FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... and further differentiated into day 3 posterior foregut through the addition of CDM3 base media supplemented with FGF2 (20 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... and then definitive ectoderm was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with BMP4 (30 ng/mL; R&D Systems), PD0325901 (100 nM ...
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bioRxiv - Genomics 2022Quote: Three million cells from each triplicate were seeded separately in 6 well-plates in fresh RPMI media and left unstimulated or stimulated with universal interferon alpha (200U/ml) (R&D systems) for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... and 10μM SB431542 for 24 hours followed by switching to SFD media supplemented with 10 μM SB431542 and 1 μM IWP2 (R&D Systems) for another 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: Human gastric and colon organoids were generated from deidentified surgical specimens from Stanford Hospital under an approved IRB protocol following established methods and grown in a submerged format in WENR media (Matano et al., 2015; Sato et al., 2011) in BME-2 extracellular matrix (R&D Systems) (Lo et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in low cluster plates to allow EB formation in serum-free differentiation SFD media supplemented with BMP4 (3 ng/ml, R&D Systems). At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614], 25 ng/mL SHH-C25II [R&D Systems, 464-SH-025] ...
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bioRxiv - Bioengineering 2023Quote: ... MVN and tumoroid treatments were administered over 4 days through daily media perfusion with an IL8 blocking antibody (20 µg mL-1, #MAB208, R&D Systems), Dexamethasone (5 µM ...
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bioRxiv - Bioengineering 2023Quote: ... media was removed and frozen for secretomic screening assays for both VEGF-A (R&D Systems, Human VEGF DuoSet ELISA, Cat# DY293B) and VEGF-C (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Cell Biology 2023Quote: The amount of VE-Cad and Rad51 protein in the conditioned media (CM) of PMVECs or human and rat plasma samples containing EDTA was quantitated by using ELISA kit (DCADV0, R&D systems for VE-Cad and custom made by MyBiosource for Rad51 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS, 1% PS) while freshly prepared media (RPMI, 10%, FBS 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM sodium pyruvate, 1% PS), while freshly prepared media (RPMI, 10% FBS, 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of TGF-β1 present in the media was quantified using the Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA kit(R&D systems). The results were normalized to the total number of cells in the culture at the moment of media collection.
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bioRxiv - Immunology 2020Quote: ... culture supernatants were collected and cytokine concentrations measured using CXCL10/IP10 (Human CXCL10/IP-10 Quantikine ELISA Kit, R&D Systems) and IFN-β (VeriKine-HS Human IFN Beta Serum ELISA Kit Cat No 41415 ...
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bioRxiv - Immunology 2019Quote: ... the cultures were supplemented with 20 μg/ml of a pan-vertebrate anti–TGF-β blocking antibody 1D11 (R&D Systems), alongside stimuli addition ...
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bioRxiv - Cancer Biology 2020Quote: Concentrations of murine IL-11 in the culture supernatants were determined by ELISA according to the manufacturers’ instruction (R&D Systems).