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Citations for R&D Systems :
101 - 150 of 1484 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and 100 ng/mL anti-HA tag antibody (clone 543851, R&D Systems) to PBMC medium and incubating for 15 min at room temperature to crosslink the proteins ...
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bioRxiv - Synthetic Biology 2024Quote: ... 2.5 µL anti-hEGF tag antibody (Anti-hEGF-biotin, R&D Systems BAF236) was added at a concentration of 0.5 µg per sample and cells incubated at 4 °C for 20 min ...
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bioRxiv - Biophysics 2024Quote: ... Purified kinetochores were incubated with biotinylated α-His antibody (R&D Systems, Minneapolis MN) at an antibody concentration of 0.025 ng/µl for 1 hour at 4 °C with rotation ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested cells were washed three times with PBS and resuspended in 2/3 Cultrex Type 2 (R&D Systems) and 1/3 Full Ad-DF+++ medium and plated in drops which were allowed to solidify for 30 minutes at 37°C after which they were overlayed with Full Ad-DF+++ medium ...
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bioRxiv - Cancer Biology 2023Quote: ... homogenized and digested in Cell Lysis Buffer #2 (R&D Systems) containing protease inhibitors on ice for 1 hour ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems, catalog # IC0501R) or HER2-ED (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... or differentiated 10T1/2 cells were treated with LRG1 (R&D Systems) for 24 h and then treated with isoproterenol (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... PBS was removed and 50 μl of cell lysis buffer (Cell Lysis Buffer 2, R&D Systems, # 895347) were added ...
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bioRxiv - Pathology 2024Quote: ... Cell surface protein expression was performed by labeling 2×105 cells for KDR-PE (R&D Systems, FAB357P) + PDGFRα-APC (Cell Signaling ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies for immunoprecipitation and Western blotting included anti-V5 and anti-His (R&D systems), Mouse anti-VAMP2 (SySy Synaptic Systems ...
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bioRxiv - Biophysics 2024Quote: ... were functionalized with biotinylated α-penta-His antibody (Qiagen, Hilden Germany or R&D Systems, Minneapolis MN) and stored in BRB80 containing 8 mg/ml BSA and 1 mM DTT at 4 °C with continuous rotation ...
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bioRxiv - Immunology 2019Quote: ... NK cells were activated for 72h in 300IU/ml rhIL-2 (R&D Systems). CD4 T cells were simultaneously activated and infected as described above ...
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bioRxiv - Immunology 2022Quote: ... activated B cells were washed and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 5 ng/ml IL-4 (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies consisted in the human anti-spike mAb 48 (1:1,000; a gift from H. Mouquet) or the mouse anti-p24 Gag MAB7360 (R&D Systems; 1:1000). Anti-human or mouse IgG secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL, R&D Systems). The readout was HRP-conjugated anti-His tag antibody recognizing the pentraxin proteins (abcam).
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bioRxiv - Developmental Biology 2023Quote: ... and a fusion of mPrtg ECD (F36-A952) to a His6 tag (Biotechne R&D Systems 6795-PR) were used as baits ...
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bioRxiv - Immunology 2021Quote: ... cells have been expanded using 20ng/ml mouse IL-2 (R&D System 402-ML). Cells were considered into exhaustion-like state after 4 stimulations (Tex ...
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bioRxiv - Immunology 2020Quote: ... isolated NK cells were cultured in 1000 IU/mL human IL-2 (R&D Systems) for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... cells were stimulated with increasing amounts of IFNs (α2 (R&D Systems, Cat#11101-2), β (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: ... cells were stimulated with increasing amounts of IFNs (α2 (R&D Systems, Cat#11101-2), β (R&D Systems ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Human ACE2 Biotinylated Antibody (Cat # BAF933) and Recombinant Spike S1 RBD protein (Cat # NBP2-90982) were obtained from R&D Systems (Minneapolis, MN, 55413) and NOVUS Biologicals (Centennial ...
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bioRxiv - Immunology 2022Quote: ... T cells were stimulated for 48 hours at 1e6 cells per ml of media containing 500 U/ml IL-2 (R&D Systems), 5 ng/ml IL-7 (R&D Systems) ...
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bioRxiv - Immunology 2022Quote: ... Recombinant Gamma (10795-CV-100) and Delta (10878-CV-100) spike proteins were purchased from R&D Systems (R&D Systems, Bio-Techne, MN, USA).
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bioRxiv - Immunology 2021Quote: ... TF-I cells medium contained 2 ng/ml of recombinant human GM-CSF (R&D Systems). Cells have been cultured for no longer than 4 weeks after thawing and were regularly tested for Mycoplasma contamination using PCR technique and were confirmed to be negative.
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bioRxiv - Cell Biology 2022Quote: ... cells were exposed for 2 days to human Activin A (8 ng/ml, R&D Systems) and human bone morphogenetic protein 4 (hBMP4 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were stimulated with increasing amounts of IFNs (α2 (R&D Systems, Cat#11101-2), β (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... Th0 cell cultures were provided IL-2 (15 U/mL; R&D Systems 202-IL-500), while Th17 cell cultures were supplemented with IL-6 (20 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then incubated with 2 μg of anti-galectin-9 (AF2545, R&D systems) or isotype control under rotation ...
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bioRxiv - Neuroscience 2024Quote: ... and 2 ng/ml glial cell-derived neurotrophic factor (GDNF, R&D Systems, Minneapolis, MN, USA). The experiments were conducted on flasks or multi-well plates (Nunc ...
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bioRxiv - Biochemistry 2022Quote: ... The peptides were serially diluted in assay buffer (PBS + 0.05 mg/mL BSA) that contained 0.16% TEA to appropriate concentrations and were preincubated with Fc-Spike-RBD (R&D Systems, Cat. No. 10542-CV-100) at 37 °C for 1 hour (12.5 μL total volume) ...
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bioRxiv - Immunology 2022Quote: ... CFSE-labelled cells were then resuspended in R10 media supplemented with recombinant IL-2 (R&D system) and 2-mercaptoethanol (Themo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The transduced cells were stained with anti-human ACE-2 polyclonal goat IgG (AF933, R&D Systems) primary antibody at 1 ug/mL and donkey anti-goat IgG conjugated to Alexa Fluor 488 (ab150129 ...
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bioRxiv - Molecular Biology 2020Quote: ... the quantity of IL-2 released by Jurkat-CD16 cells was measured in the cell supernatant by colorimetry using a human IL-2 DuoSet ELISA DY202-05 Kit (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Immunology 2023Quote: ... and 50 U/mL Interleukin-2 (IL-2, R&D Systems). Pre-isolated NK cells were thawed and co-cultured at a 1:1 effector:target (E:T ...
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bioRxiv - Cell Biology 2022Quote: ... Type 2 (BME-2) droplets (R&D Systems, Minneapolis, MN, USA) and covered under medium ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were embedded in Cultrex® RGF BME Type 2 (cat no. 3533-005-02, R&D systems) and placed in a 37°C incubator for 20 min ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse tumors were homogenized with a tissue homogenizer in 700μL of Cell Lysis Buffer 2 (R&D Systems) containing 1x Halt Protease Inhibitor Cocktail (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were maintained in proliferating state using 10 ng/ml fibroblast growth factor 2 (FGF2; R&D Systems) and passaged twice before usage in the experiments ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were grown in DMEM/F12 (HyClone, SH30023.02) supplemented with 2 %v/v N21 (R&D system, AR008), 20 ng/mL EGF (R&D system ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured in the presence of IL-2 (5 ng/ml; R&D Systems, Minneapolis, MN, USA), and in the presence or the absence of CH-223191 or FICZ ...
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bioRxiv - Cancer Biology 2022Quote: ... Control or shLATS1/2 cells were counted and resuspended in 1% methylcellulose medium (R&D system Cat. # HSC001) prepared in complete growth medium ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were stimulated with 2 ng/mL recombinant human TGF-β1 (R&D Systems, cat. # 240-B) diluted in starvation medium for varying durations of 30 ...
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bioRxiv - Microbiology 2021Quote: ... The Hoc proteins each eluted as a single monomeric peak and were run on reducing SDS-PAGE and verified by anti-His western (R&D Systems) (Supplementary Fig.3) ...
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bioRxiv - Biophysics 2022Quote: ... ECD fragments (modified C-terminally with 10xHis tag) Podocalyxin (PODXL) (Cat.# 1658-PD) and PODXL2 (Cat.# 1524-EG) were obtained from R&D Systems. The ECD fragments were dissolved in ultrapure water to a concentration of 0.5 mg/mL and aliquoted in 50 μL ...
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bioRxiv - Immunology 2019Quote: ... and macrophage inflammatory protein-2 (MIP-2) (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... DuoSet IC ELISA kit (DYC1767-2 and DYC1766-2, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and 2 ng/ml IL-2 (carrier-free; Tocris R&D Systems). Cells treated with only these functioned as the mock control ...
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bioRxiv - Immunology 2022Quote: ... T cells then were washed and further incubated with recombinant human IL-2 (10 ng/ml; R&D systems) for up to 2 weeks at 37°C incubator with medium change every 3 days ...
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bioRxiv - Cell Biology 2020Quote: ... Autophagy was induced by treating the cells for 2 hours in 250nM Torin-1 (R&D Systems, 4247/10) in 1X Hanks’ Balanced salt solution ...
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bioRxiv - Molecular Biology 2022Quote: ... CH12F3-2 cells were incubated in culture medium supplemented with10ng/mL IL-4 (R&D Systems #404-ML-050), 1ng/mL TGFβ (R&D Systems #7666-MB-005 ...