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101 - 150 of 2597 citations for Recombinant Human CTGF Protein His Avi tagged Biotinylated since 2019

• In-utero exposure to estrogen-mimicking bisphenols alters bone mineralization in the offspring

  Saikanth Varma, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Recombinant human Noggin protein-carrier free (#6057-NG/CF) was obtained from R&D systems.
• Development of a novel, pan-variant aerosol intervention for COVID-19

  Robert H. Shoemaker, et al., bioRxiv - Pharmacology and Toxicology 2021

  Quote: ... Biotinylated goat anti-human ACE2 (R&D Systems) diluted in blocking buffer was added to plates at 0.4 µg/ml followed by a 1 h incubation at r.t ...
• Essential roles of plexin-B3⁺ oligodendrocyte precursor cells in the pathogenesis of Alzheimer’s disease

  Naomi Nihonmatsu-Kikuchi, et al., bioRxiv - Neuroscience 2020

  Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
• Relative Mutant N501Y SARS-CoV-2 Spike Protein RBD Inhibition of Anti-Spike Protein IgG and ACE-2 Binding to Spike Protein Species

  Melvin E. Klegerman, et al., bioRxiv - Biochemistry 2021

  Quote: Human anti-SP IgG or ACE-2 Fc was mixed with recombinant SARS-CoV-2 spike RBD His tag (R&D Systems) or recombinant SARS-CoV-2 spike RBD (N501Y)-His tag (Sino Biological ...
• A multiadjuvant polysaccharide-amino acid-lipid (PAL) subunit nanovaccine generates robust systemic and lung-specific mucosal immune responses against SARS-CoV-2 in mice

  Bhawana Pandey, et al., bioRxiv - Bioengineering 2023

  Quote: ... Plates were incubated with PBSTBA diluted 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) for 2 h at RT ...
• Evolution of limb development in cephalopod mollusks

  Oscar A. Tarazona, et al., bioRxiv - Developmental Biology 2019

  Quote: ... were selected and transferred to 1mg/ml recombinant human Noggin protein (6057-NG, R&D Systems) in PBS and incubated for 30 minutes to 1 hour at ambient temperature before being implanted in embryos ...
• High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity

  Jinghua Lu, Peter D. Sun, bioRxiv - Biochemistry 2020

  Quote: Recombinant proteins of human ACE2 extracellular enzymatic domain were purchased from R&D systems(933-ZN) and Sinobiological ...
• Molecular Crowding Facilitates Bundling of IMPDH Polymers and Cytoophidium Formation

  Chia-Chun Chang, et al., bioRxiv - Cell Biology 2022

  Quote: Same amount (1 μM) of purchased Human IMPDH2 recombinant protein (8349-DH-050, R&D systems) was incubated in 50 mM HEPES buffer (pH 8.5 ...
• PP2A and GSK3 act as modifiers of FUS-ALS by modulating mitochondrial transport

  Paraskevi Tziortzouda, et al., bioRxiv - Neuroscience 2023

  Quote: ... and 0.01 mg/mL recombinant human agrin protein (R&D Systems, cat. no. 6624-AG-050). On the same day ...
• Early steps of multi-receptor viral interactions dissected by high-density, multi-color single molecule mapping in living cells

  Nicolas Mateos, et al., bioRxiv - Biophysics 2023

  Quote: ... were obtained from BD Biosciences. Recombinant human Gal-9 protein (Cat. number 9064-GA) was obtained from R&D systems. SARS-CoV-2 Spike Protein (RBD ...
• Targeting GFPT2 to reinvigorate immunotherapy in EGFR-mutated NSCLC

  Luyao Ao, et al., bioRxiv - Cancer Biology 2024

  Quote: ... samples were treated with recombinant human PD-1 Fc protein (1086-PD-050, R&D System) at 4 °C overnight ...
• Microglia-specific promoter activities of human HexB gene

  Sahil Shah, et al., bioRxiv - Neuroscience 2021

  Quote: ... recombinant human M-CSF (R&D Systems), 100 μg/mL streptomycin ...
• A secreted Echinococcus multilocularis activin A homologue promotes regulatory T cell expansion

  Justin Komguep Nono, Manfred B. Lutz, Klaus Brehm, bioRxiv - Immunology 2019

  Quote: ... Recombinant human TGF-β1 (R&D Systems) was used as positive control ...
• Therapeutic modulation of the blood-brain barrier and ischemic stroke by a bioengineered FZD₄-selective WNT surrogate

  Jie Ding, et al., bioRxiv - Physiology 2022

  Quote: ... and recombinant human Norrin (R&D Systems) were used as response comparators ...
• Spatially discrete signalling niches regulate fibroblast heterogeneity in human lung cancer

  CJ Hanley, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Human Recombinant TGF-β1 (R&D Systems) was reconstituted in 4mM HCl ...
• Schistosoma mansoni cercarial invadolysin cleaves complement C3 and suppresses proinflammatory cytokine production

  Jacob R. Hambrook, Patrick C. Hanington, bioRxiv - Immunology 2022

  Quote: ... recombinant human MMP-8 (R&D Systems) was subjected to the same conditions ...
• Schistosoma mansoni cercarial invadolysin cleaves complement C3 and suppresses proinflammatory cytokine production

  Jacob R. Hambrook, Patrick C. Hanington, bioRxiv - Immunology 2022

  Quote: Recombinant human Eotaxin-1 (R&D systems) and recombinant IL-5 (R&D systems ...
• Nuclear lamina strain states revealed by intermolecular force biosensor

  Brooke E. Danielsson, et al., bioRxiv - Biophysics 2022

  Quote: ... recombinant human TGF-β1 (R&D systems) was used to induce EMT at a concentration of 2 ng/mL for 24 h ...
• Spatio-temporal analysis of LC3 association to Mycobacterium tuberculosis phagosomes in human macrophages

  Jacques Augenstreich, et al., bioRxiv - Cell Biology 2022

  Quote: ... Recombinant human Interferon Gamma (R&D Systems), OADC supplement (R&D Systems) ...
• Fusobacterium nucleatum induces pancreatic cancer cell proliferation and migration by regulating host autocrine and paracrine signaling

  Barath Udayasuryan, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Human GM-CSF biotinylated antibody (R&D Systems, BAM215) was added to the medium to a final concentration of 90 ng/ml and incubated at room temperature with gentle shaking for 3 hours ...
• Single dose immunization with a COVID-19 DNA vaccine encoding a chimeric homodimeric protein targeting receptor binding domain (RBD) to antigen-presenting cells induces rapid, strong and long-lasting neutralizing IgG, Th1 dominated CD4⁺ T cells and strong CD8⁺ T cell responses in mice

  Gunnstein Norheim, et al., bioRxiv - Microbiology 2020

  Quote: ... either biotinylated anti-human MIP-1α (R&D Systems) or SARS-CoV-2/2019-nCoV Spike/RBD Antibody (1:1000 ...
• Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast

  Daniel J. Barrero, et al., bioRxiv - Biophysics 2024

  Quote: ... Purified kinetochores were incubated with biotinylated α-His antibody (R&D Systems, Minneapolis MN) at an antibody concentration of 0.025 ng/µl for 1 hour at 4 °C with rotation ...
• The histone modifier KAT2A presents a selective target in a subset of well-differentiated microsatellite-stable colorectal cancers

  Vida Kufrin, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Human recombinant EGF (20 ng/mL) and human recombinant FGF (10 ng/mL) (R&D Systems, Minneapolis, MN, USA) were added twice per week.
• Distinct effects of different matrix proteoglycans on collagen fibrillogenesis and cell-mediated collagen reorganization

  Dongning Chen, et al., bioRxiv - Cell Biology 2020

  Quote: ... USA) and human recombinant lumican protein (lacking GAG chains) was from R&D Systems (Minneapolis, MN, USA). CS sodium salt isolated from bovine cartilage and ChABC from Proteus vulgaris were from Sigma ...
• Structure and function of the ROR2 cysteine-rich domain in vertebrate noncanonical WNT5A signaling

  Samuel C. Griffiths, et al., bioRxiv - Biochemistry 2021

  Quote: The following recombinant proteins and drugs were purchased: human/mouse WNT5A (654-WN-010, R&D Systems); Wnt-C59 (C7641-2s ...
• Altered MAM function shifts mitochondrial metabolism in SOD1-mutant models of ALS

  Delfina Larrea, et al., bioRxiv - Molecular Biology 2022

  Quote: ... 10 ng/ml recombinant human brain-derived neurotrophic factor (BDNF) protein (R&D Systems 248-BDB-005), and 10 µM ɣ-secretase inhibitor (DAPT ...
• The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signaling

  Diego Jaime, et al., bioRxiv - Cell Biology 2022

  Quote: ... then treated with 20-25 ng/ml recombinant human BMP4 protein (R&D Systems, Cat# 314-BP). For acetylcholine receptor clustering experiments ...
• Anti-TNF-induced IL-27 modulates regulatory T cell responses in patients with IBD that respond to therapy

  Raphael S. Peres, et al., bioRxiv - Immunology 2022

  Quote: ... Recombinant Human IL-27 Ra/WSX-1/TCCR Fc Chimera Protein (2 μg/ml, R&D Systems), Ultrapure LPS from E ...
• Propionate reinforces epithelial identity and reduces aggressiveness of non-small cell lung carcinoma via chromatin remodelling

  Vignesh Ramesh, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Inhibitors used in the study are Recombinant Human TGF-β1 protein (R&D Systems, 240-B-002), SAHA (Sigma ...
• High-resolution low-cost LCD 3D printing of microfluidics

  Houda Shafique, et al., bioRxiv - Bioengineering 2024

  Quote: ... recombinant human interferon-γ protein (Cat. #285-IF, lot #RAX2422031, R&D Systems, Minneapolis, Minnesota, United States), Pierce streptavidin poly-horseradish peroxidase (pHRP ...
• Bioactive coatings on 3D printed scaffolds for bone regeneration: Translation from in vitro to in vivo models and the impact of material properties and growth factor concentration

  Karen. M. Marshall, et al., bioRxiv - Bioengineering 2023

  Quote: ... human fibronectin and human recombinant BMP-2 (R&D systems, Biotechne, UK); recombinant human BMP-2 (Infuse/InductOS® Bone graft kit ...
• State-dependent protein-protein interactions mediating 4-1BB CAR Signaling

  Samuel A. Ritmeester-Loy, et al., bioRxiv - Immunology 2022

  Quote: CD19 recombinant protein (R&D Systems) or anti-CD3 (Biolegend ...
• “Neighbourhood watch” model: embryonic epiblast cells assess positional information in relation to their neighbours

  Hyung Chul Lee, et al., bioRxiv - Developmental Biology 2022

  Quote: Recombinant human BMP4 (R&D systems, 312-BP) was delivered using Affigel Blue beads (BIO-RAD 1537302) ...
• Combinatorial T cell engineering eliminates on-target off-tumor toxicity of CD229 CAR T cells while maintaining anti-tumor activity

  Erica R. Vander Mause, et al., bioRxiv - Bioengineering 2022

  Quote: ... and 0.25µM recombinant human CD229 (R&D Systems) in kinetics buffer ...
• Aspartate reduces liver inflammation and fibrosis by suppressing the NLRP3 inflammasome pathway via upregulating NS3TP1 expression

  Li Zhou, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Human recombinant TGF-β1 from R&D Systems Inc ...
• TGF-β signaling and Creb5 cooperatively regulate Fgf18 to control pharyngeal muscle development

  Jifan Feng, et al., bioRxiv - Developmental Biology 2022

  Quote: ... recombinant human TGFβ1 (7754-BH, R&D Systems) was used at 5 ng/ml for the TGFβ1 treatment group for 24 hours ...
• The second lineage differentiation of bovine embryos fails in the absence of OCT4/POU5F1

  Kilian Simmet, et al., bioRxiv - Developmental Biology 2021

  Quote: ... human recombinant FGF4 (R&D Systems, Minneapolis, Minnesota) and heparin (Merck ...
• Irisin stimulates the release of CXCL1 from differentiating human subcutaneous and deep-neck derived adipocytes via upregulation of NFκB pathway

  Abhirup Shaw, et al., bioRxiv - Cell Biology 2021

  Quote: ... recombinant human CXCL1 (275-GR, R&D Systems) was used at 2500 pg/mL concentration in starvation media ...
• Parallel Activation of Src and Hif1α Increases Localized Glycolytic ATP Generation for Re-assembly of Endothelial Adherens Junctions

  Li Wang, et al., bioRxiv - Cell Biology 2022

  Quote: ... and human recombinant TNFα from R&D systems. Small interfering RNAs (siRNAs ...
• Nuclear receptor ligand screening in an iPSC-derived in vitro blood-brain barrier model identifies new contributors to leptin transport

  Yajuan Shi, et al., bioRxiv - Neuroscience 2022

  Quote: Recombinant human leptin (R&D Systems #398-LP) was reconstituted at 1 mg/mL in sterile 20 mM Tris-HCl (Fisher Scientific #MT46031CM ...
• Characterisation of the functional and transcriptomic effects of pro-inflammatory cytokines on human EndoC-βH5 beta cells

  Caroline Frørup, et al., bioRxiv - Cell Biology 2022

  Quote: ... and/or recombinant human TNFα (R&D Systems).
• High prevalence and dependence of centrosome clustering in mesenchymal tumors and leukemia

  N. Moreno-Marin, et al., bioRxiv - Cancer Biology 2023

  Quote: Recombinant human TGFβ1 (240-B R&D Systems) was stocked as 20 μg/ml in DMSO and AZ3146 (SML-1427 Sigma Aldrich ...
• HDL regulates TGFß-receptor lipid raft partitioning, restoring contractile features of cholesterol-loaded vascular smooth muscle cells

  Prashanth Thevkar Nagesh, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and recombinant human TGFβ-1 (R&D Systems) were performed.
• Bioactive coatings on 3D printed polycaprolactone scaffolds for bone regeneration: a novel murine femur defect model for examination of the biomaterial capacity for repair

  Karen. M. Marshall, et al., bioRxiv - Bioengineering 2023

  Quote: ... recombinant human fibronectin (R&D systems, Biotechne, UK); Laponite (BYK) ...
• Physiologic Doses of TGF-β Improve the Composition of Engineered Articular Cartilage

  Tianbai Wang, et al., bioRxiv - Bioengineering 2023

  Quote: ... human recombinant active TGF-β3 (R&D Systems) was supplemented in culture media at specified concentrations for the initial 14 days of culture.
• Autoimmune antibody-induced neuronal hyperactivity triggers pathological Tau in IgLON5 disease

  Bilge Askin, et al., bioRxiv - Neuroscience 2024

  Quote: Recombinant human IgLON5-Fc chimera (R&D Systems) was bound to a 1 ml HiTrap® NHS- activated HP column (GE Healthcare ...
• Measurement of adhesion and traction of cells at high yield (MATCHY) reveals an energetic ratchet driving nephron condensation

  Jiageng Liu, et al., bioRxiv - Bioengineering 2024

  Quote: ... recombinant human GFRA1 (R&D Systems, AF714-SP) along with 50 ng/ml recombinant human GDNF (R&D Systems ...
• Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccine

  Alexandra Atalis, et al., bioRxiv - Immunology 2022

  Quote: ... plates were incubated for 2 h at RT with 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) diluted in PBSTBA ...
• Unique molecular characteristics of visceral afferents arising from different levels of the neuraxis: location of afferent somata predicts function and stimulus detection modalities

  Kimberly A. Meerschaert, et al., bioRxiv - Neuroscience 2020

  Quote: ... recombinant mouse interferon-γ and recombinant human interkeukin-8 (R&D Systems, Minneapolis, MN) was prepared in PBS containing 0.1% BSA ...
• Fusobacterium nucleatum host cell binding and invasion induces IL-8 and CXCL1 secretion that drives colorectal cancer cell migration

  Michael A. Casasanta, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Human IL-8/CXCL8 biotinylated antibody (R&D Systems BAF208) and Human/Primate CXCL1/GROα/KC/CINC-1 biotinylated antibody (R&D Systems BAF275 ...