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101 - 150 of 2649 citations for Recombinant Human CD200 Protein Fc Avi tagged Biotinylated since 2019

• Neuronal pentraxin Nptx2 regulates complement activity in the brain

  Jiechao Zhou, et al., bioRxiv - Neuroscience 2022

  Quote: ... were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL, R&D Systems). The readout was HRP-conjugated anti-His tag antibody recognizing the pentraxin proteins (abcam).
• Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants

  Yi-Nan Zhang, et al., bioRxiv - Immunology 2021

  Quote: ... Recombinant human IL-2 protein was purchased from R&D Systems (Minneapolis, MN). Recombinant mouse Flt3 ligand (Flt3L ...
• LPHN2 inhibits vascular permeability by differential control of endothelial cell adhesion

  Chiara Camillo, et al., bioRxiv - Cell Biology 2021

  Quote: ... Recombinant human carrier-free FLRT2 protein (2877-FL) was from R&D Systems. Streptavidin-conjugated HRP was from GE Healthcare.
• Melanoma cells release DEL-1 via small extracellular vesicles

  Marianne Schnödl, et al., bioRxiv - Cancer Biology 2022

  Quote: ... recombinant human DEL-1 protein (6046-ED) (R&D Systems, Minneapolis, MN, USA) and/or RGD peptide (A8052 ...
• Tumor necrosis factor-α modulates GABAergic and Dopaminergic neurons in the ventral periaqueductal gray of female mice

  Dipanwita Pati, Thomas L. Kash, bioRxiv - Neuroscience 2021

  Quote: ... Recombinant human TNF-α protein was purchased from R&D Systems (Minneapolis, USA) and stored at -20°C.
• Coiled coil control of growth factor and inhibitor-dependent EGFR trafficking and degradation

  Deepto Mozumdar, et al., bioRxiv - Biochemistry 2021

  Quote: ... Recombinant Human TGF-a Protein (#293-A) were purchased from R&D Systems. Mini-PROTEAN® TGXTM Precast Gels (10% polyacrylamide ...
• Single-component, self-assembling, protein nanoparticles presenting the receptor binding domain and stabilized spike as SARS-CoV-2 vaccine candidates

  Linling He, et al., bioRxiv - Microbiology 2021

  Quote: ... Recombinant human IL-2 protein was purchased from R&D Systems (Minneapolis, MN). Recombinant mouse Flt3 ligand (Flt3L ...
• High and low permeability of human pluripotent stem cell-derived Blood Brain barrier models depend on epithelial or endothelial features

  Stéphane D. Girard, et al., bioRxiv - Neuroscience 2022

  Quote: ... 25 µg/ml human recombinant Bone Morphogenetic Protein 4 (BMP4, R&D Systems) and 55 µM β-mercaptoethanol (Life technologies) ...
• LRP1 and SORL1 regulate tau internalization and degradation and enhance tau seeding

  Joanna M. Cooper, et al., bioRxiv - Neuroscience 2020

  Quote: ... Recombinant human SORL1 aa 82-753 protein was purchased from R&D Systems.
• Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae, to the liver

  Hitoshi Tsugawa, et al., bioRxiv - Microbiology 2023

  Quote: ... recombinant human Gas6 protein (R&D Systems, Minneapolis, MN, USA, cat# 885-GSB), recombinant mouse Gas6 protein (R&D Systems ...
• Multi-omic network analysis identified betacellulin as a novel target of omega-3 fatty acid attenuation of western diet-induced nonalcoholic steatohepatitis

  Jyothi Padiadpu, et al., bioRxiv - Systems Biology 2023

  Quote: ... BTC at 40 ng/mL (human recombinant Betacellulin protein, R&D systems, MN), or combinations of all for 6 hours ...
• High CD44 expression identifies rare chemoresistant leukemic cells endowed with enhanced E-Selectin binding in T-ALL

  Julien Calvo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... recombinant E-selectin– human-IgG1 fusion protein (724 ES 100, R&D Systems) was pre-complexed at 5µg/mL with PE-conjugated goat anti-Human IgG diluted at 1/100e (Supplementary Table 2 ...
• Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatse Sulf-2

  Aswini Panigrahi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Recombinant human galectin 3 binding protein (R&D Systems, and produced in-house), QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies) ...
• Endothelial FABP4 constitutes the majority of basal circulating hormone levels and regulates lipolysis-driven insulin secretion

  Karen E. Inouye, et al., bioRxiv - Physiology 2022

  Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
• ATGL is differentially required for adipocyte FABP4 secretion in vivo and ex vivo

  Kacey J. Prentice, et al., bioRxiv - Physiology 2022

  Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
• Discovery and functional interrogation of SARS-CoV-2 protein-RNA interactions

  Joy S. Xiang, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Recombinant His-tagged NSP12 (R&D Systems, catalog # 10686-CV) was incubated with in vitro transcribed and biotinylated RNA in 20 mM Tris ...
• A non-ligand surrogate agonist antibody that enhances canonical Wnt signaling and bone regeneration

  Nam-Kyung Lee, et al., bioRxiv - Bioengineering 2022

  Quote: ... Protein A biosensors (ForteBio) were loaded with human LRP6-ECD-Fc (R&D Systems) for 120 sec ...
• Lentiviral vector optimization enhances the expression and cytotoxicity of chimeric antigen receptors

  Changjiang Guo, et al., bioRxiv - Cell Biology 2021

  Quote: ... NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems, USA) and then FITC-conjugated anti-human Fc secondary antibody was used as a detection antibody (BD Biosciences ...
• An unconventional cerebrospinal fluid-derived Semaphorin-signalling regulates apical progenitor dynamics in the developing neocortex

  Katrin Gerstmann, et al., bioRxiv - Neuroscience 2020

  Quote: ... we incubated brain hemispheres with recombinant Sema3B-Alkaline Phosphatase in the presence or absence of recombinant Nrp1-Fc or Nrp2-Fc (R&D systems), respectively (10 ng/µl in PBS) ...
• DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells

  Yale S. Michaels, et al., bioRxiv - Bioengineering 2021

  Quote: ... and 2.5ug/mL Fc-tagged VCAM1 (R&D Systems, 643-VM). Tissue culture-treated 96 well plates (Fisher Scientific ...
• Time- and lineage-resolved transcriptional profiling uncovers gene expression programs and clonal relationships that underlie human T lineage specification

  Yale S. Michaels, et al., bioRxiv - Immunology 2023

  Quote: ... Fc-tagged VCAM1 (2.5 μg/ml; R&D Systems, 643-VM) and Fc-tagged DLL4 (15 μg/ml ...
• Exploiting the Cullin E3 Ligase Adaptor Protein SKP1 for Targeted Protein Degradation

  Seong Ho Hong, et al., bioRxiv - Biochemistry 2023

  Quote: ... The SKP1 containing SCF complex was reconstituted by utilizing stoichiometric amount of the following recombinant proteins: Recombinant Human CUL1/RBX1 Neddylated Complex Protein (R&D systems, E3-411-025), Recombinant Human FBXO7 GST (N-Term ...
• The GPI sidechain of Toxoplasma gondii prevents parasite pathogenesis

  Julia A Alvarez, et al., bioRxiv - Microbiology 2024

  Quote: ... or recombinant CD36-Fc (R&D Systems, #2519-CD) in 50μl binding buffer (0.14 M NaCl ...
• Cell surface receptors TREM2, CD14 and integrin α_Mβ₂ drive sinking engulfment in phosphatidylserine-mediated phagocytosis

  Daan Vorselen, et al., bioRxiv - Cell Biology 2022

  Quote: ... Membranes were incubated in the same buffer for 1 h under light agitation with the recombinant murine chimera proteins containing human Fc domains and the extracellular domains of TREM2 (R&D systems, 1729-T2-050, 0.1 mg/mL) or CD14 (R&D systems ...
• The Initiation Knot is a Signaling Center Required for Molar Tooth Development

  Isabel Mogollón, et al., bioRxiv - Cell Biology 2020

  Quote: ... were incubated with 100μg/ml recombinant human Wnt10b protein (0.1mg/ml;R&D Systems) at 37°C for 30 minutes ...
• Electro-mechanical transfection for non-viral primary immune cell engineering

  Jessica M. Sido, et al., bioRxiv - Bioengineering 2021

  Quote: ... 100M PBMCs were thawed in 100 mL X-VIVO™ 10 media from Lonza (#04-380Q) with recombinant human IL-2 protein from R&D Systems (#202-IL). After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971 ...
• Decorin, a novel negative modulator of E-cadherin in inflammatory breast cancer

  Xiaoding Hu, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Recombinant human decorin protein (#143-DE-100) was purchased from R&D Systems (USA). Anti-decorin (#HPA064736) ...
• Balancing the length of distal tip is key for stability and signalling function of primary cilia

  Taishi Kanamaru, et al., bioRxiv - Cell Biology 2021

  Quote: ... Recombinant Human SHH Protein for SHH pathway activation was purchased from R&D systems.
• Subclinical Atherosclerosis is Associated with Discrepancies in BAFF and APRIL Levels and Altered Breg Potential of Precursor-like Marginal Zone B-Cells in HIV Treated Individuals

  Matheus Aranguren, et al., bioRxiv - Immunology 2022

  Quote: ... in absence or presence of APRIL (Recombinant Human APRIL/TNFSF13 Protein, R&D systems) at 50 ng/ml ...
• ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulation

  Mohamed Abu-Farha, et al., bioRxiv - Biochemistry 2023

  Quote: ... the cells were treated with Recombinant Human TNFα Protein (R&D Systems, MN, USA) at 25ng/mL for 18 hours ...
• The developing mouse coronal suture at single-cell resolution

  D’Juan T. Farmer, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and Cd200 (1:200; AF2724, R&D systems) or Tcf7 (1:200 ...
• Alveolar cell fate selection and lifelong maintenance of AT2 cells by FGF signaling

  Douglas G. Brownfield, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Fgfr2iiib-Fc (human, R&D Systems) and Phospho-p44/42 MAPK (rabbit ...
• LFA-1 signals to promote actin polymerization and upstream migration in T cells

  Nathan H Roy, et al., bioRxiv - Cell Biology 2020

  Quote: ... Recombinant mouse ICAM-1-Fc and VCAM-1-Fc were purchased from R&D Systems. Antibodies for flow cytometery were from BioLegend ...
• Antisense modulation of IL7R splicing to control sIL7R expression in human CD4⁺ T cells

  Gaddiel Galarza-Muñoz, et al., bioRxiv - Immunology 2022

  Quote: ... The concentration of samples was extrapolated from standard curves of recombinant human IL7R-Fc chimera (R&D Systems, # 306-IR).
• Primate-specific response of astrocytes to stroke limits peripheral macrophage infiltration

  Anthony G. Boghdadi, et al., bioRxiv - Neuroscience 2020

  Quote: ... or with human NogoA (566-748aa) and Fc chimera protein (Nogo-Δ20; R&D Systems), for 10m ...
• AI-based antibody discovery platform identifies novel, diverse and pharmacologically active therapeutic antibodies against multiple SARS-CoV-2 strains

  Cristina Moldovan Loomis, et al., bioRxiv - Immunology 2023

  Quote: ... were coated with human ACE-2 Fc chimera protein (R&D Systems cat# 10544-ZN) in cold PBS (100 μl per well ...
• Mfge8 attenuates human gastric antrum smooth muscle contractions

  Wen Li, et al., bioRxiv - Physiology 2020

  Quote: Recombinant human Mfge8 and recombinant human laminin subunit alpha-1 were purchased from R&D Systems, www.rndsystems.com ...
• The Role of Notch Signaling in Endometrial Mesenchymal Stromal/Stem-like Cells Maintenance

  Sisi Zhang, et al., bioRxiv - Cell Biology 2022

  Quote: Human recombinant JAG1 (R&D System) was fluorescently labeled with Alexa Fluor™ 488 Microscale Protein Labeling Kit (Thermo Scientific ...
• Superantigens promote Staphylococcus aureus bloodstream infection by eliciting pathogenic interferon-gamma (IFNγ) production that subverts macrophage function

  Stephen W. Tuffs, et al., bioRxiv - Microbiology 2021

  Quote: ... recombinant human IFNγ (R&D Systems) was also added at varying concentrations ...
• Non-canonical open reading frames encode functional proteins essential for cancer cell survival

  John R. Prensner, et al., bioRxiv - Cancer Biology 2020

  Quote: Recombinant human GDF15 (R&D Systems, Minneapolis ...
• The Polycomb group protein Ring1 regulates dorsoventral patterning of the mouse telencephalon

  Hikaru Eto, et al., bioRxiv - Developmental Biology 2019

  Quote: ... recombinant human BMP4 (R&D Systems) or CHIR-99201 (Wako) ...
• Wnt7a-releasing synthetic hydrogel enhances local skeletal muscle regeneration and muscle stem cell engraftment

  Woojin M. Han, et al., bioRxiv - Bioengineering 2019

  Quote: ... recombinant human Wnt7a (R&D systems) reconstituted in PBS was added to the PEG-4MAL precursor solution ...
• Investigating vaccine uptake dynamics of neutrophils using HIV-1 Envelope glycoprotein trimer

  Philip Y.X. Ngo, bioRxiv - Immunology 2022

  Quote: ... Recombinant human MBL (R&D systems) was reconstituted in PBS and serially diluted from 10μg/ml by two-fold dilution ...
• Therapeutic modulation of the blood-brain barrier and ischemic stroke by a bioengineered FZD₄-selective WNT surrogate

  Jie Ding, et al., bioRxiv - Physiology 2022

  Quote: ... Recombinant human WNT3A (R&D Systems) and recombinant human Norrin (R&D Systems ...
• Self-organization, quality control, and preclinical studies of human iPSC-derived retinal sheets for tissue-transplantation therapy

  Kenji Watari, et al., bioRxiv - Neuroscience 2023

  Quote: ... recombinant human BMP4 (R&D Systems) was added at 1.5 nM (55 ng/ml)7 ...
• Suspension Electrospinning of Decellularized Extracellular Matrix

  Sarah Jones, et al., bioRxiv - Bioengineering 2024

  Quote: ... Recombinant human VEGF (R&D Systems) was added to the basal media at 20 ng/mL immediately prior to cell treatment ...
• Single-cell atlas of major haematopoietic tissues sheds light on blood cell formation from embryonic endothelium

  Maya Shvartsman, et al., bioRxiv - Developmental Biology 2019

  Quote: ... 0.01% of recombinant human FGF basic protein 10μg/ml (R&D Systems, #233-FB-025).
• Opposing immune and genetic forces shape oncogenic programs in synovial sarcoma

  Livnat Jerby-Arnon, et al., bioRxiv - Cancer Biology 2019

  Quote: ... Recombinant Human EGF Protein 10 ng/mL (R&D systems, Cat. No. 236-EG-200), Recombinant Human FGF basic ...
• BRCA1-BARD1 regulates transcription through modulating topoisomerase IIβ

  Jaehyeon Jeong, et al., bioRxiv - Molecular Biology 2020

  Quote: ... and BARD1 proteins was conducted using recombinant human ubiquitin (U-100H, R&D system, USA) and HeLa NE as a source of E1 and E2 ligases in 20 mM Tris-HCl pH 7.5 ...
• BRAF inhibitor resistance confers increased sensitivity to mitotic inhibitors

  SA Misek, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Recombinant human TNFα protein (#210-TA-005) was purchased from R&D Systems (Minneapolis, USA).