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Citations for R&D Systems :
651 - 700 of 2576 citations for Recombinant Human Aldehyde Dehydrogenase 3 Family MemberA1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Llamas were immunized intramuscularly once per week over six weeks with carrier free recombinant human IL-9 or mouse IL-9 (R&D Systems). Each llama (4 in total ...
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bioRxiv - Developmental Biology 2023Quote: ... EBs were transferred into 60 mm non-adhesive dishes and differentiated in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems) for 3 days ...
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bioRxiv - Genetics 2023Quote: ... The calibrator supplied with the kit by Ansh Labs is traceable to recombinant human GDF-15 from R&D Systems (Biotechne, USA). Ansh Lab ELISA Total GDF15 between batch imprecision kit controls 7.7% at 173.2 pg/ml ...
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bioRxiv - Cell Biology 2023Quote: M.tb-Lux was exposed to control- or HIV-ALF with or without (as control) supplemented human recombinant SP-D (5 μg/mL, R&D Systems). After exposure ...
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bioRxiv - Immunology 2023Quote: ... The culture was then diluted 1:50 in 1X PBS + 1% LB broth and incubated statically at 37°C with recombinant human SLPI protein (rSLPI) (CAS: 1274-PI-100, R&D Systems) at these final concentrations ...
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bioRxiv - Microbiology 2023Quote: ... This polyUb fraction was then incubated with either 1) 100 nM human recombinant PolyHistidine-otubain 1 (R&D systems, Cat# E-522B), 2 ...
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bioRxiv - Cancer Biology 2024Quote: ... GPC2 CAR expression was determined using APC or PE-conjugated human recombinant GPC2 protein (R&D Systems; 2304-GP-050; 1:500) and CD19 CAR using PE-tagged protein L (58036S ...
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bioRxiv - Biochemistry 2022Quote: ... or 1:1000 anti-Human/Mouse/Rat PRL-3 Antibody (R&D Systems, MAB3219, Lot. WXH0419091). Following three washes with 0.1% TBST ...
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bioRxiv - Microbiology 2022Quote: ... and the Human IL-29/IL-28B (IFNλ1/3) DuoSet ELISA (DY1589, R&D Systems, USA) kit according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... hBTLA-6×His tag (R&D systems) and hLIGHT-6×His tag proteins (made by the methods described above ...
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bioRxiv - Biophysics 2024Quote: ... Anti-HIS antibody (R&D systems, BAM050) coated polystyrene beads were prepared as previously described.39 The beads were decorated with native purified Dsn1-6HIS-3Flag kinetochores ...
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bioRxiv - Immunology 2021Quote: ... recombinant MBL (R&D systems) (100 µg mL-1 ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant LOXL2 (R&D Systems) was used as a positive control.
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bioRxiv - Neuroscience 2021Quote: ... The contribution of BDNF/TrkB signaling was tested by bath application of a scavenger of endogenous BDNF (recombinant human TrkB-Fc chimera, R&D Systems, Germany). For scavenging of BDNF ...
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bioRxiv - Genomics 2019Quote: ... as from previous dose-response experiments3–5: recombinant human IL-1β (specific activity 1.8×107 U/mg; 201-LB-005, R&D Systems, Abingdon, UK) at 50 U/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... Fc Tag 15μg/ml (CDO-H5257, Acro Biosystem Newark, USA) or Recombinant Human IgG1 Fc 3μg/ml (110-HG, R&D Systems, Minneapolis, MN, USA) as control ...
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bioRxiv - Immunology 2020Quote: ... Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems; R15-100). R15-100 was added every 2 to 3 days thereafter ...
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bioRxiv - Immunology 2021Quote: The expression of NKG2D ligands in tumor cell lines was assessed using Recombinant Human NKG2D Fc Chimera Protein (R&D systems, Abingdon, UK). 105 tumor cells were incubated either with 0.5 μg of NKG2D Fc recombinant protein or IgG1-Fc during 30 min ...
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bioRxiv - Cell Biology 2022Quote: MC3T3 cells were cultured in growth media and treated with increasing concentrations of recombinant human CXCL12/SDF-1α (R&D Systems, #350-NS) reconstituted in PBS and 0.1% BSA in a 24-well trans-well plate and allowed to migrate for 3 hours prior to cell quantification.
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bioRxiv - Neuroscience 2023Quote: ... the cells were cultivated in neural progenitor medium based on 2D-NIM medium without the dual SMAD inhibitors SB-431542 and LDN-193189 and supplemented with 20 ng/mL recombinant human basic FGF (#233-FB, R&D Systems, Germany) and 10 µM Y-27632 (only for the first 24 h after passaging) ...
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bioRxiv - Immunology 2023Quote: ... in PBS and HUVECs were plated at confluence and were stimulated with 40 ng/ml recombinant human TNF-α (210-TA; R&D Systems) and/or 50 ng/ml IFN-γ (285-IF ...
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bioRxiv - Bioengineering 2023Quote: ... Plates were incubated with PBSTBA diluted 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) for 2 h at RT ...
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bioRxiv - Physiology 2023Quote: ... ciCMVEC were placed in serum-free media 1h before treatment with 10 ng/ml of Recombinant Human TNF-α Protein (210-TA-005; R&D systems) or vehicle (PBS ...
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bioRxiv - Genomics 2024Quote: ... 5 mM beta-glycerophosphate and 1% insulin-transferrin-selenous acid (osteogenic media) and stimulated them the next day with recombinant human BMP2 (300 ng/ml) (R&D Systems, MN) in serum-free osteogenic media ...
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bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... washed twice and resuspended in T-cell migration media (RPMI + 1% FBS) with or without recombinant human CXCL12 (R&D Systems, 350-NS). PBMC (50,000 ...
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bioRxiv - Systems Biology 2023Quote: Where indicated the cells were stimulated with the final concentration of 10 ng/ml of recombinant human TNF-α protein (R&D Systems) or 100 ng/ml of recombinant human interleukin 1 beta (IL-1β ...
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bioRxiv - Synthetic Biology 2020Quote: ... Myc-tagged and V5-tagged CARs were stained using an Alexa Fluor 488-conjugated mouse anti-myc antibody (R&D Systems, IC3696G) and an Alexa Fluor 647-conjugated mouse anti-V5 antibody (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... Recombinant DLL4 (#1389-D4) and recombinant Jagged-1 (#Q63722) were purchased from R&D systems and coated on plates at the same time as anti-CD3/anti-CD28 ...
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bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL human Fms-related tyrosine kinase 3 ligand (Flt3L, #308-FK, all R&D Systems), 2 mM L-GlutaMAX (Gibco #35050-038) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was quantified using Human Dkk-3 DuoSet Solid Phase Sandwich ELISA (DY1118, R&D Systems, MN), per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used were rabbit anti-Human Phospho-Smad2/3 (R&D Systems MAB8935, 1:500 dilution), goat anti-histone (Abcam ab12079 ...
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bioRxiv - Bioengineering 2024Quote: ... which was supplemented with 3 ng/mL human activin A (R&D Systems, Inc., Minneapolis, MN, USA), 1 ng/mL human bone morphogenetic protein 4 (Bmp4 ...
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bioRxiv - Immunology 2020Quote: ... the plate was washed 3 times and incubated with 80 µL per well of monoclonal mouse IgG2B human sRAGE or galectin-3 antibody (R&D Systems, Minneapolis, Minnesota, United States) at a concentration of 0.2 µg/ml for 30 minutes shaking at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... for 24 hours and rested overnight in C-RPMI with 100µM ferric ammonium citrate (FeAC) and 200U/ml of human recombinant IFN-γ (R&D Systems, MN USA) unless otherwise stated.
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bioRxiv - Developmental Biology 2020Quote: Embryonic immortalised mouse epicardium-derived cells (mEPDC) were treated with 2.0 µg/ml human recombinant transforming growth factor (TGF)-β (R&D Systems; 240-B) for 30 hrs to induce EMT ...
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bioRxiv - Developmental Biology 2019Quote: ... Both wild type and Adgrg6 KO ATDC5 cells were treated with 100ng/ml recombinant human IL-6 protein (rIL-6) (R&D System, 206-IL) for 2 hours before protein extraction.
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bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
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bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Human ACE2 Biotinylated Antibody (Cat # BAF933) and Recombinant Spike S1 RBD protein (Cat # NBP2-90982) were obtained from R&D Systems (Minneapolis, MN, 55413) and NOVUS Biologicals (Centennial ...
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bioRxiv - Cancer Biology 2022Quote: ... +/-1 µg/mL anti-CD28 (CD28.2) antibody (eBioscience/Thermo 16-0289-81) or recombinant human CD58-Fc chimera protein (R&D Systems 10068-CD-050) at 37 ºC for 2 hours ...
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bioRxiv - Bioengineering 2020Quote: ... Microvascular fragment-containing gels were cultured in serum-free media supplemented with 10 ng/mL recombinant human vascular endothelial growth factor (rhVEGF; R&D Systems, Minneapolis, MN) (55) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were serum starved overnight and then incubated with 10 ng/ml recombinant human transforming growth factor-beta 1 (TGF-β1) (R&D Systems, Minneapolis, MN) for indicated times.
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bioRxiv - Genetics 2020Quote: ... culture medium was replaced with KSR medium (Chambers et al., 2009, 2011) supplemented with 200 ng/ml of recombinant human Noggin (R&D Systems, Minneapolis, USA) and 10 μM of SB431542 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 5 hours in DermaLife Basal Medium followed by stimulation with human recombinant IL-17A (R&D systems, 50 ng/ml) and TNF-α (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... Five million PBMC were suspended in 40 ml R10 supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050) together with 10 million K562-mbIL21-41BBL feeder cells irradiated with 100 Gy ...
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bioRxiv - Neuroscience 2022Quote: ... a mix of 10 μg/mL Laminin and 5 μg/mL of each SLRP protein (recombinant human CHAD (R&D Systems Cat#8218-CH), FMOD (R&D Systems Cat#9840-FM) ...
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bioRxiv - Physiology 2023Quote: ... 1nM) in VEH for 60 minutes prior to the addition of recombinant human TGF-β1 (5 ng/ml; R&D Systems, Minneapolis, MN, USA). At 6 ...
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bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Experiments investigating EV-free AREG were undertaken by incubating recipient cells with 0.03 – 3000 ng/ml recombinant human Amphiregulin protein (R&D Systems #262-AR-100), for 30 minutes at 37°C before plating as described above.