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1 - 50 of 2557 citations for Recombinant Human ABHD15 His tagged since 2020

• Turncoat antibodies unmasked in a model of autoimmune demyelination: from biology to therapy

  Reza Taghipour-Mirakmahaleh, et al., bioRxiv - Immunology 2024

  Quote: ... His-tagged recombinant human and mouse FcγRs (R&D Systems) were diluted to 2 μg/ml in HBS-EP+ (10 mM HEPES ...
• Lack of blocking activity in anti-CTLA-4 antibodies reduces toxicity, but not anti-tumor efficacy

  Erica L. Stone, et al., bioRxiv - Immunology 2021

  Quote: ... Recombinant human His-tagged CD80 (R&D Systems 9050-B1, Minneapolis, MN, USA) or CD86 (R&D Systems 9090-B2 ...
• Discovery and functional interrogation of SARS-CoV-2 protein-RNA interactions

  Joy S. Xiang, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Recombinant His-tagged NSP12 (R&D Systems, catalog # 10686-CV) was incubated with in vitro transcribed and biotinylated RNA in 20 mM Tris ...
• A Gallium-68-Labeled Peptide Radiotracer For CD38-Targeted Imaging In Multiple Myeloma With PET

  Ajay Kumar Sharma, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The ligands used were His-Tagged human CD38 (R&D systems, catalog # 2404-AC ...
• NECTIN-4 PET FOR OPTIMIZING ENFORTUMAB VEDOTIN DOSE-RESPONSE IN UROTHELIAL CARCINOMA

  Akhilesh Mishra, et al., bioRxiv - Cancer Biology 2024

  Quote: ... The ligands used were His-Tagged human Nectin-4 (R&D systems, catalog # 2659-N4-050 ...
• Substrate identification and specificity profiling of deubiquitylases against endogenously-generated ubiquitin-protein conjugates

  Valentina Rossio, et al., bioRxiv - Biochemistry 2023

  Quote: ... Single human recombinant DUBs (800 nM) were added together with 50 μM human recombinant HA-tagged ubiquitin (R&D SYSTEMS, U110) to each reaction ...
• SorCS1 inhibits amyloid-β binding to neurexin and rescues amyloid-β-induced synaptic pathology

  Alfred Kihoon Lee, et al., bioRxiv - Neuroscience 2022

  Quote: ... The pre-immobilized beads were then incubated with 50 nM recombinant mouse SorCS1 ectodomain tagged with a C-terminal 6-His tag (SorCS1-His, Cat# 4395-SR-050, R&D systems) in ECS for 2 h at 4 °C ...
• Therapeutic Targeting of TIM-4-L With Engineered T Cells for Acute Myeloid Leukemia

  Brandon Cieniewicz, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and recombinant human TIM-4-HIS (R&D Systems, Cat# 9407-TM-050)) ...
• Neuronal pentraxin Nptx2 regulates complement activity in the brain

  Jiechao Zhou, et al., bioRxiv - Neuroscience 2022

  Quote: ... were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL, R&D Systems). The readout was HRP-conjugated anti-His tag antibody recognizing the pentraxin proteins (abcam).
• The SATB1-MIR22-GBA axis mediates glucocerebroside accumulation inducing a cellular senescence-like phenotype in dopaminergic neurons

  Taylor Russo, et al., bioRxiv - Neuroscience 2023

  Quote: ... cells were treated for 24h with 100ng/ml media of human recombinant GBA (rhGBA-His (R&D Systems)).
• The extracellular matrix fibulin 7 maintains epidermal stem cell heterogeneity during skin aging

  Erna Raja, et al., bioRxiv - Developmental Biology 2022

  Quote: ... 0.03 to 3 μg/well of the soluble mouse or human HA-tagged recombinant fibulin 7 (R&D Systems) in 2% dry milk/TBS and 2 mM CaCl2 were added to the wells and incubated for 3 hours ...
• The Hao-Fountain syndrome protein USP7 regulates neuronal connectivity in the brain via a novel p53-independent ubiquitin signaling pathway

  Hao Chen, et al., bioRxiv - Neuroscience 2023

  Quote: ... Either 1µL 500nM recombinant His-USP7 (R&D system) or 1µL deubiquitination buffer as vehicle control was added to each reaction and incubated for 1 hour at 37 °C ...
• Endothelial FABP4 constitutes the majority of basal circulating hormone levels and regulates lipolysis-driven insulin secretion

  Karen E. Inouye, et al., bioRxiv - Physiology 2022

  Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
• ATGL is differentially required for adipocyte FABP4 secretion in vivo and ex vivo

  Kacey J. Prentice, et al., bioRxiv - Physiology 2022

  Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
• Enhancing extracellular vesicle cargo loading and functional delivery by engineering protein-lipid interactions

  Justin A. Peruzzi, et al., bioRxiv - Bioengineering 2023

  Quote: ... purified FLAG tagged protein (recombinant p53 protein, R&D Systems) was run with vesicles to quantify loading ...
• Non-glycosylated IGF2 prohormones are more mitogenic than native IGF2

  Pavlo Potalitsyn, et al., bioRxiv - Biochemistry 2023

  Quote: ... His-tagged ALS protein (R&D Systems, 10 ng/well, 1.5 µM) was immobilized in wells via neutravidin and the nickel-charged-NTA-decorated iBodies® (IOCB Tech ...
• Trogocytic-molting of T-cell microvilli controls T-cell clonal expansion

  Jeong-Su Park, et al., bioRxiv - Immunology 2022

  Quote: ... Anti-human CD28 antibody and recombinant mouse ICAM-1/CD54 His-tag were purchased from R&D Systems (Minneapolis, MN, USA). Hybridoma cell lines for mouse anti-CD3 (145-2C11 ...
• Mfge8 attenuates human gastric antrum smooth muscle contractions

  Wen Li, et al., bioRxiv - Physiology 2020

  Quote: Recombinant human Mfge8 and recombinant human laminin subunit alpha-1 were purchased from R&D Systems, www.rndsystems.com ...
• The Role of Notch Signaling in Endometrial Mesenchymal Stromal/Stem-like Cells Maintenance

  Sisi Zhang, et al., bioRxiv - Cell Biology 2022

  Quote: Human recombinant JAG1 (R&D System) was fluorescently labeled with Alexa Fluor™ 488 Microscale Protein Labeling Kit (Thermo Scientific ...
• Superantigens promote Staphylococcus aureus bloodstream infection by eliciting pathogenic interferon-gamma (IFNγ) production that subverts macrophage function

  Stephen W. Tuffs, et al., bioRxiv - Microbiology 2021

  Quote: ... recombinant human IFNγ (R&D Systems) was also added at varying concentrations ...
• Non-canonical open reading frames encode functional proteins essential for cancer cell survival

  John R. Prensner, et al., bioRxiv - Cancer Biology 2020

  Quote: Recombinant human GDF15 (R&D Systems, Minneapolis ...
• Self-organization, quality control, and preclinical studies of human iPSC-derived retinal sheets for tissue-transplantation therapy

  Kenji Watari, et al., bioRxiv - Neuroscience 2023

  Quote: ... recombinant human BMP4 (R&D Systems) was added at 1.5 nM (55 ng/ml)7 ...
• Therapeutic modulation of the blood-brain barrier and ischemic stroke by a bioengineered FZD₄-selective WNT surrogate

  Jie Ding, et al., bioRxiv - Physiology 2022

  Quote: ... Recombinant human WNT3A (R&D Systems) and recombinant human Norrin (R&D Systems ...
• Suspension Electrospinning of Decellularized Extracellular Matrix

  Sarah Jones, et al., bioRxiv - Bioengineering 2024

  Quote: ... Recombinant human VEGF (R&D Systems) was added to the basal media at 20 ng/mL immediately prior to cell treatment ...
• Investigating vaccine uptake dynamics of neutrophils using HIV-1 Envelope glycoprotein trimer

  Philip Y.X. Ngo, bioRxiv - Immunology 2022

  Quote: ... Recombinant human MBL (R&D systems) was reconstituted in PBS and serially diluted from 10μg/ml by two-fold dilution ...
• Novel mechanism of inflammatory activation by Ebola virus matrix protein linked to the ebolavirus virulence

  Satoko Yamaoka, et al., bioRxiv - Microbiology 2024

  Quote: ... recombinant human TNFα (R&D Systems) (0.05 ng ...
• Inhibition of EGFR/ErbB does not protect against C. difficile toxin B

  Uswah Siddiqi, et al., bioRxiv - Microbiology 2024

  Quote: ... Recombinant human EGF (R&D Systems) was applied similarly as a positive control ...
• An Adipo-Pulmonary Axis Mediated by FABP4 Hormone Defines a Therapeutic Target Against Obesity-Induced Airway Disease

  M. Furkan Burak, et al., bioRxiv - Physiology 2024

  Quote: ... Human recombinant FABP4 (R&D Systems) was used as a standard ...
• Essential roles of plexin-B3⁺ oligodendrocyte precursor cells in the pathogenesis of Alzheimer’s disease

  Naomi Nihonmatsu-Kikuchi, et al., bioRxiv - Neuroscience 2020

  Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
• Relative Mutant N501Y SARS-CoV-2 Spike Protein RBD Inhibition of Anti-Spike Protein IgG and ACE-2 Binding to Spike Protein Species

  Melvin E. Klegerman, et al., bioRxiv - Biochemistry 2021

  Quote: Human anti-SP IgG or ACE-2 Fc was mixed with recombinant SARS-CoV-2 spike RBD His tag (R&D Systems) or recombinant SARS-CoV-2 spike RBD (N501Y)-His tag (Sino Biological ...
• Identification of a new regulation pathway of EGFR and E-cadherin dynamics

  Veronique Proux-Gillardeaux, et al., bioRxiv - Cell Biology 2020

  Quote: 0.3M of purified proteins (recombinant human E-cadherin 8505 and recombinant human EGFR 344-ER R&D system) were mixed and incubated in 100μl of lysis buffer (25 mM TrisHCl pH 7.5 ...
• Microglia-specific promoter activities of human HexB gene

  Sahil Shah, et al., bioRxiv - Neuroscience 2021

  Quote: ... recombinant human M-CSF (R&D Systems), 100 μg/mL streptomycin ...
• Cooperative regulation of adherens junction elongation through Epidermal Growth Factor Receptor (EGFR) activation

  Chaoyu Fu, et al., bioRxiv - Biophysics 2021

  Quote: ... Recombinant Human EGF Protein (R&D Systems). Erlotinib hydrochloride (500nM ...
• Therapeutic modulation of the blood-brain barrier and ischemic stroke by a bioengineered FZD₄-selective WNT surrogate

  Jie Ding, et al., bioRxiv - Physiology 2022

  Quote: ... and recombinant human Norrin (R&D Systems) were used as response comparators ...
• Spatially discrete signalling niches regulate fibroblast heterogeneity in human lung cancer

  CJ Hanley, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Human Recombinant TGF-β1 (R&D Systems) was reconstituted in 4mM HCl ...
• Schistosoma mansoni cercarial invadolysin cleaves complement C3 and suppresses proinflammatory cytokine production

  Jacob R. Hambrook, Patrick C. Hanington, bioRxiv - Immunology 2022

  Quote: ... recombinant human MMP-8 (R&D Systems) was subjected to the same conditions ...
• Schistosoma mansoni cercarial invadolysin cleaves complement C3 and suppresses proinflammatory cytokine production

  Jacob R. Hambrook, Patrick C. Hanington, bioRxiv - Immunology 2022

  Quote: Recombinant human Eotaxin-1 (R&D systems) and recombinant IL-5 (R&D systems ...
• Nuclear lamina strain states revealed by intermolecular force biosensor

  Brooke E. Danielsson, et al., bioRxiv - Biophysics 2022

  Quote: ... recombinant human TGF-β1 (R&D systems) was used to induce EMT at a concentration of 2 ng/mL for 24 h ...
• Dynamic Interplay of Autophagy and Membrane Repair During Mycobacterium tuberculosis Infection

  Jacques Augenstreich, et al., bioRxiv - Cell Biology 2024

  Quote: ... Recombinant human Interferon Gamma (R&D Systems), OADC supplement (R&D Systems) ...
• Biosynthetic characterization and combinatorial biocatalysis of the cysteine protease inhibitor E-64

  Mengting Liu, et al., bioRxiv - Biochemistry 2024

  Quote: ... Recombinant human procathepsin B (R&D systems) was activated to mature cathepsin B by incubation at 37 °C for 20 min in activation buffer (20 mM Na-acetate pH 5.5 ...
• The histone modifier KAT2A presents a selective target in a subset of well-differentiated microsatellite-stable colorectal cancers

  Vida Kufrin, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Human recombinant EGF (20 ng/mL) and human recombinant FGF (10 ng/mL) (R&D Systems, Minneapolis, MN, USA) were added twice per week.
• PD-1 signaling uncovers a pathogenic subset of T cells in inflammatory arthritis

  Johanna Straube, et al., bioRxiv - Immunology 2023

  Quote: ... recombinant human PD-L1 human IgG1 Fc chimera protein (R&D Systems), or mouse IgG1 isotype antibodies (R&D Systems ...
• Bioactive coatings on 3D printed scaffolds for bone regeneration: Translation from in vitro to in vivo models and the impact of material properties and growth factor concentration

  Karen. M. Marshall, et al., bioRxiv - Bioengineering 2023

  Quote: ... human fibronectin and human recombinant BMP-2 (R&D systems, Biotechne, UK); recombinant human BMP-2 (Infuse/InductOS® Bone graft kit ...
• Thrombospondin 2 is a key determinant of fibrogenesis in NAFLD

  Takefumi Kimura, et al., bioRxiv - Molecular Biology 2023

  Quote: The recombinant human transforming growth factor beta (TGFβ) protein and recombinant human TSP2 protein were obtained from R&D Systems. The SMAD3 Inhibitor SIS3 was procured from Cayman Chemical Company ...
• “Neighbourhood watch” model: embryonic epiblast cells assess positional information in relation to their neighbours

  Hyung Chul Lee, et al., bioRxiv - Developmental Biology 2022

  Quote: Recombinant human BMP4 (R&D systems, 312-BP) was delivered using Affigel Blue beads (BIO-RAD 1537302) ...
• Combinatorial T cell engineering eliminates on-target off-tumor toxicity of CD229 CAR T cells while maintaining anti-tumor activity

  Erica R. Vander Mause, et al., bioRxiv - Bioengineering 2022

  Quote: ... and 0.25µM recombinant human CD229 (R&D Systems) in kinetics buffer ...
• Aspartate reduces liver inflammation and fibrosis by suppressing the NLRP3 inflammasome pathway via upregulating NS3TP1 expression

  Li Zhou, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Human recombinant TGF-β1 from R&D Systems Inc ...
• TGF-β signaling and Creb5 cooperatively regulate Fgf18 to control pharyngeal muscle development

  Jifan Feng, et al., bioRxiv - Developmental Biology 2022

  Quote: ... recombinant human TGFβ1 (7754-BH, R&D Systems) was used at 5 ng/ml for the TGFβ1 treatment group for 24 hours ...
• The second lineage differentiation of bovine embryos fails in the absence of OCT4/POU5F1

  Kilian Simmet, et al., bioRxiv - Developmental Biology 2021

  Quote: ... human recombinant FGF4 (R&D Systems, Minneapolis, Minnesota) and heparin (Merck ...
• Irisin stimulates the release of CXCL1 from differentiating human subcutaneous and deep-neck derived adipocytes via upregulation of NFκB pathway

  Abhirup Shaw, et al., bioRxiv - Cell Biology 2021

  Quote: ... recombinant human CXCL1 (275-GR, R&D Systems) was used at 2500 pg/mL concentration in starvation media ...