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Citations for R&D Systems :
1 - 50 of 1329 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant His-tagged NSP12 (R&D Systems, catalog # 10686-CV) was incubated with in vitro transcribed and biotinylated RNA in 20 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human His-tagged CD80 (R&D Systems 9050-B1, Minneapolis, MN, USA) or CD86 (R&D Systems 9090-B2 ...
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bioRxiv - Neuroscience 2022Quote: ... The pre-immobilized beads were then incubated with 50 nM recombinant mouse SorCS1 ectodomain tagged with a C-terminal 6-His tag (SorCS1-His, Cat# 4395-SR-050, R&D systems) in ECS for 2 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL, R&D Systems). The readout was HRP-conjugated anti-His tag antibody recognizing the pentraxin proteins (abcam).
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bioRxiv - Bioengineering 2024Quote: ... His-tagged mouse TfR (TfR cross-reactivity, R&D Systems 9706-TR-050 ...
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bioRxiv - Cancer Biology 2023Quote: ... The ligands used were His-Tagged human CD38 (R&D systems, catalog # 2404-AC ...
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bioRxiv - Neuroscience 2023Quote: ... Either 1µL 500nM recombinant His-USP7 (R&D system) or 1µL deubiquitination buffer as vehicle control was added to each reaction and incubated for 1 hour at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... purified FLAG tagged protein (recombinant p53 protein, R&D Systems) was run with vesicles to quantify loading ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged ALS protein (R&D Systems, 10 ng/well, 1.5 µM) was immobilized in wells via neutravidin and the nickel-charged-NTA-decorated iBodies® (IOCB Tech ...
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bioRxiv - Cell Biology 2019Quote: ... and 1µg of recombinant human E-cadherin (R&D systems). Coverslips were then washed two times with PBS.
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bioRxiv - Cell Biology 2020Quote: 0.3M of purified proteins (recombinant human E-cadherin 8505 and recombinant human EGFR 344-ER R&D system) were mixed and incubated in 100μl of lysis buffer (25 mM TrisHCl pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... BSA (24 - 48 h at 4°C in rotation) for HIS-tagged rPtxS1 and rGαi proteins with mouse monoclonal anti-HIS (1:1000) (R&D Systems, MAB050), for Gαi with mouse monoclonal anti-Gαi (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... 3) 50 nM recombinant PolyHistidine-CYLD (R&D systems, Cat# E-556), or 50nM recombinant SdeC DUB (1-192 ...
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bioRxiv - Biochemistry 2023Quote: ... Single human recombinant DUBs (800 nM) were added together with 50 μM human recombinant HA-tagged ubiquitin (R&D SYSTEMS, U110) to each reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant human TIM-4-HIS (R&D Systems, Cat# 9407-TM-050)) ...
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bioRxiv - Microbiology 2023Quote: ... 2) 50 nM recombinant USP2 catalytic domain (R&D systems, Cat# E-504), 3 ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant E-selectin– human-IgG1 fusion protein (724 ES 100, R&D Systems) was pre-complexed at 5µg/mL with PE-conjugated goat anti-Human IgG diluted at 1/100e (Supplementary Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... coli were incubated with serial dilutions of HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Immunology 2023Quote: ... 5 µg/ml of recombinant mouse Siglec-E-Fc chimera (R&D Systems, Cat. 5806) in 100 μl of buffer (10mM Tris-HCl (pH7.4) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... MA) and recombinant mouse PD-1 His-tag protein was from R&D Systems (Minneapolis, MN). Glycine ...
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bioRxiv - Bioengineering 2021Quote: Recombinant human IDO expressed in Escherichia coli was purchased from R&D Systems (Minneapolis, MN) with a predicted molecular mass of 46 kDa a specific activity of >500pmoles/min/μg (or for equimolar calculations >29 pmol NFK/min/pmol IDO ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant human ubiquitin-activating enzyme E1 (UBE1) was purchased from R&D systems (Cat. # E-305). Recombinant human UbcH5a was purchased from R&D systems (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... and recombinant mouse E-cadherin (cat. no. 8875-EC-050) were purchased from R&D Systems. A fragment of human Plexin-B1 cDNA (encoding amino acids 1-535 ...
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bioRxiv - Cell Biology 2024Quote: ... Recombinant Human His6-SENP1 Catalytic Domain (SENP1, Catalog no. E-700) was from R&D Systems.
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bioRxiv - Biophysics 2022Quote: ... the gels were washed twice with 1xPBS and incubated at room temperature with a drop of 25μL of 0.01mg·mL-1 histidine- tagged E-cadherin (8505-EC-050, R&D Systems) covered with rectangles of parafilm ...
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bioRxiv - Immunology 2022Quote: ... 6.25 ng pMHC and 6.25 ng recombinant mouse PD-L1 with 6-His tag (R&D systems) in 2% BSA were injected into each well in this step ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant human SUMO activating enzyme E1 (SAE1/UBA2) was purchased from R&D systems (Cat. # E-315) Recombinant Human Ubc9 was purchased from (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... 10μM of compound E or 4μg/ml of recombinant Fc-JAG1 (R&D Systems, Cat #599-JG). After the EU labelling ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.03 to 3 μg/well of the soluble mouse or human HA-tagged recombinant fibulin 7 (R&D Systems) in 2% dry milk/TBS and 2 mM CaCl2 were added to the wells and incubated for 3 hours ...
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bioRxiv - Neuroscience 2023Quote: ... cells were treated for 24h with 100ng/ml media of human recombinant GBA (rhGBA-His (R&D Systems)).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... HepCV E (His tag, 9146-HC) and CD81-Fc (9144-CD) were from R&D Systems (Minneapolis, MN, USA); IL-2 (His tag ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was also measured using 5 nM of recombinant human 26S proteasome (Cat: E-365; R&D Systems).
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bioRxiv - Microbiology 2023Quote: ... coli were made from an overnight agar culture and incubated with 3 μM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Biochemistry 2020Quote: In vitro co-precipitation of TREM2 or TREM1 with Aβ oligomers was performed by mixing recombinant Fc-tagged TREM2 or TREM1 ectodomain (residues 19-171; 100 ng/ml, R&D systems), 100 nM HiLyte Fluor 647-labelled Aβ oligomers and Protein G Dynabeads in 0.5% fatty acid free BSA PBS-Tween buffer ...
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bioRxiv - Immunology 2022Quote: ... Anti-human CD28 antibody and recombinant mouse ICAM-1/CD54 His-tag were purchased from R&D Systems (Minneapolis, MN, USA). Hybridoma cell lines for mouse anti-CD3 (145-2C11 ...
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bioRxiv - Cancer Biology 2019Quote: ... Active CD73 enzyme (Recombinant Human 5’-Nucleotidase/CD73 Protein, CF) and active CD39 (His-Tag Human) were from R&D Systems, Minneapolis ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from twice passaged nutrient broth cultures and incubated with 3 uM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Neuroscience 2020Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
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bioRxiv - Biochemistry 2021Quote: Human anti-SP IgG or ACE-2 Fc was mixed with recombinant SARS-CoV-2 spike RBD His tag (R&D Systems) or recombinant SARS-CoV-2 spike RBD (N501Y)-His tag (Sino Biological ...
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bioRxiv - Cancer Biology 2022Quote: ... live/dead stained and incubated with recombinant human Siglec-9 Fc chimera (#1139-SL-050) or recombinant mouse Siglec-E Fc chimera (#5806-SL-050) both from R&D systems, USA for 20 min on ice ...
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bioRxiv - Microbiology 2023Quote: ... This polyUb fraction was then incubated with either 1) 100 nM human recombinant PolyHistidine-otubain 1 (R&D systems, Cat# E-522B), 2 ...
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bioRxiv - Immunology 2020Quote: ... Cells were plated in 96-well plate in density of 1×106/mL in 200μL of medium and cultured at 37°C and 5% CO2 for 48h in presence of 5μg of lipopolysaccharide (LPS, isolated from E. coli strain 055:B5, Sigma, St. Louis, MO, USA; LBP from R&D Systems, Abingdon, UK), isotype control (rat IgG2b ...
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bioRxiv - Cell Biology 2024Quote: ... heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis.
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bioRxiv - Cell Biology 2019Quote: ... E-cadherin (R&D Systems) and BSA (Fisher) ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... coli (R&D Systems) with recombinant EGFR Fc-fusion (R&D Systems) ...
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bioRxiv - Cancer Biology 2020Quote: ... His (R&D Systems MAB050-100), COX2 (Abcam ab15191) ...
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bioRxiv - Cancer Biology 2020Quote: ... His (R&D Systems MAB050-100). Coverslips were incubated with the primary antibody at 4°C overnight and then washed five times for 5 minutes each in PBS following which ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant experiments were carried out by using F9 recombinant protein (R&D systems). MCF7 cell line was treated with 10 μg/ml in complete medium for two rounds of 72 hours (total of 6 days treatment) ...