Labshake search
Citations for R&D Systems :
201 - 248 of 248 citations for Rat SEPT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Sections were incubated overnight with unconjugated goat anti-mouse/rat CD47 N-terminal IgV-like extracellular domain antibody (R&D Systems), biotin-conjugated anti-mouse CD55 REAfinity antibody (clone REA300 ...
-
bioRxiv - Genomics 2023Quote: ... anti-FcγRII/III (rat anti-mouse, clone 190909, cat. MAB1460), and anti-FcγRI (rat anti-mouse, clone 29035, cat. MAB2074) were purchased from R&D systems. 40μg of anti-FcγRII/III and 20μg of anti-FcγRI was used in FcγR-blocking conditions ...
-
bioRxiv - Microbiology 2024Quote: ... Cytokine and chemokines levels were measured using a commercial multiplex immunoassay (Proteome Profiler rat XL Cytokine Array Kit; R&D system) which allows the detection of 79 rat cytokines and chemokines according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Activin A levels were measured using an ELISA kit (Human/Mouse/Rat Activin A Quantikine ELISA Kit, R&D Systems, DAC00B).
-
bioRxiv - Cell Biology 2023Quote: ... Chicken anti pan-neurofascin (against residues 25-1031 of rat neurofascin, 1:400 for IF) was from R&D System (#AF3235). Rabbit polyclonal anti-clathrin heavy chain (against residues 1650 to C-terminus of human clathrin heavy chain ...
-
bioRxiv - Neuroscience 2023Quote: ELISA kits were used to determine the levels of GDF15 (mouse/rat GDF15 Quantikine ELISA Kit, R&D Systems, catalog # MGD150), TIMP-1 (mouse TIMP-1 Quantikine ELISA Kit ...
-
bioRxiv - Microbiology 2021Quote: ... Serum concentrations of IGF-1 and IGFBP-3 were determined using the Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems) and IGFBP-3 DuoSet ELISA kit (R&D Systems) ...
-
bioRxiv - Immunology 2020Quote: ... We used commercially available ELISA kits to quantify plasma IFNγ (mouse IFNγ platinum ELISA extra sensitive, ebioscience), CXCL10 (mouse IP-10 platinum ELISA, ebioscience) and Angiopoietin-2 (mouse/rat Angiopoietin-2 quantine ELISA kit, R&D Systems). Absorbance was measured using a Multiskan Ascent (MTX Lab systems ...
-
bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
-
bioRxiv - Cancer Biology 2019Quote: ... media was removed from astrocytes and levels of cytokines were measured using the Rat XL Cytokine Array Kit (R&D Systems ARY030) using 1 mL of conditioned media per membrane ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were then treated with 200ng/mL of recombinant rat NogoA (1026-1090aa) and Fc Chimera protein (Nogo-66; R&D Systems), or with human NogoA (566-748aa ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein expression for the expressed human LTα transgene was measured in snap-frozen rat CSF using a human TNF-beta/TNFSF1 Quantikine ELISA kit (DTB00, R&D systems) as per manufacturer’s instructions.
-
bioRxiv - Neuroscience 2021Quote: ... The cells were immunostained with rat anti-GFP (1:2000, GF090R; Nacalai Tesque) and goat anti-TrkB (1:250, AF1494; R&D Systems). Cells were extensively washed and incubated with appropriate secondary antibodies at 4°C overnight ...
-
bioRxiv - Molecular Biology 2020Quote: The release of IL-6 by lung tissues to the culture medium was measured using Rat IL-6 DuoSet ELISA kit (R&D Systems) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend]; goat anti-myeloperoxidase [goatMPO, AF3667, R&D Systems] ...
-
bioRxiv - Immunology 2020Quote: ... 1.25% of supernatant from Con-A-stimulated rat splenocytes (assay positive control), c) recombinant canine IL-4 (rcaIL-4, 360 ng/mL) (R&D Systems), d ...
-
bioRxiv - Cell Biology 2022Quote: The concentration of active TGF-β1 was measured using the Human /Mouse /Rat /Porcine /Canine TGF-beta 1 Quantikine ELISA kit (DB100B, R&D Systems). Briefly ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Total CREB and pCREB were measured using the Human/Mouse/Rat Phospho-CREB (S133) Cell-based ELISA (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2019Quote: ... Primary antibodies (1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and goat anti-PDX1 (R&D Systems; AF2419) were left on overnight ...
-
bioRxiv - Immunology 2020Quote: ... Quantitation of IL-2 muteins in mouse serum was performed using an electrochemiluminescent immunoassay with a biotinylated rat anti-mouse IL-2 (JES6-1A12, R&D Systems) as a capture reagent and a ruthenylated goat anti-mouse IgG ...
-
bioRxiv - Cell Biology 2021Quote: LECs were pre-cultured with FBS-free and supplement-free basal medium for 24 h and then treated with medium containing 100 ng/mL of recombinant rat CTGF (R&D Systems) for 5 min ...
-
bioRxiv - Bioengineering 2020Quote: Surface expression of decoy constructs on engineered MSC lines was assessed by using either APC-conjugated rat-anti-mouse gp130 (IL6ST) antibodies (clone FAB4681A, R&D Systems) or AlexaFluor647-conjugated mouse-anti-human CD120a (TNFR1 ...
-
bioRxiv - Physiology 2022Quote: ... Blood FGF21 levels were assayed by a mouse/rat FGF-21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA).
-
FGF21 has a sex-specific role in calorie-restriction-induced beiging of white adipose tissue in micebioRxiv - Physiology 2022Quote: ... Blood FGF21 levels were assayed by a mouse/rat FGF-21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA).
-
bioRxiv - Physiology 2022Quote: ... Blood FGF21 levels were assayed by a mouse/rat FGF-21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA).
-
bioRxiv - Cell Biology 2023Quote: The amount of VE-Cad and Rad51 protein in the conditioned media (CM) of PMVECs or human and rat plasma samples containing EDTA was quantitated by using ELISA kit (DCADV0, R&D systems for VE-Cad and custom made by MyBiosource for Rad51 ...
-
bioRxiv - Molecular Biology 2024Quote: ... containing 0.1% FBS (Hyclone Laboratories Inc.)] and stimulated with 10 ng/mL rat PDGF-AA ligand (R&D Systems, Minneapolis, MN, USA) diluted from a 1.5 μg/mL working solution in 40 nM HCl containing 0.1% BSA for the indicated length of time ...
-
bioRxiv - Cell Biology 2024Quote: ... The amount of TGF-β1 present in the media was quantified using the Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA kit(R&D systems). The results were normalized to the total number of cells in the culture at the moment of media collection.
-
bioRxiv - Neuroscience 2021Quote: ... Nrp1-Fc fusion protein containing the extracellular domain of rat Nrp1 (amino acid 22-854 minus 811-828; R&D Systems, 566-NNS) or the control human Fc dimer (R&D Systems ...
-
bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies have been used in this study: Anti-Human/Mouse/Rat/Chicken Pax7 (5 μg/ml, R&D Systems, MAB1675), Anti-Human/Mouse Myod1 (5.8A ...
-
bioRxiv - Immunology 2023Quote: Cytokine levels of TNFα was quantified in serum obtained from mice infected with and without Salmonella for 24 h by the mouse/rat TNF-A Valukine ELISA kit (R&D Systems, #VAL609) following the product protocol.
-
bioRxiv - Biochemistry 2023Quote: ... Serum Cystatin C levels were measured using the Mouse/Rat Cystatin C Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, MSCTC0). Blood Nitrogen Urea serum levels were measured using the QuantiChrom Urea Assay Kit (BioAssay Systems ...
-
bioRxiv - Immunology 2023Quote: ... T cells were analyzed using ELISpot according to the manufacturer’s protocol ELISpot Rat interferon-gamma (IFNγ) (Cat: EL585 by R&D Systems, United States).
-
bioRxiv - Cancer Biology 2024Quote: ... Sections from formalin-fixed paraffin-wax embedded tumours were stained with primary rat anti-mouse monoclonal anti-CD8 antibody (eBioscience, 4Sm15as) and goat anti-mouse polyclonal anti-PD-L1 antibody (R&D systems, AF1019). Slides were scanned and imaged using Hamamatsu Nanozoomer (Hamamatsu Photonics).
-
bioRxiv - Neuroscience 2024Quote: ... MaxiSORP 96-well plates were coated with 1μg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (R&D Systems; clone 237920) at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: FGF-21 levels in the serum and liver were determined using Quantikine mouse/rat FGF-21 ELISA kit from R&D systems (MN, USA). Serum and liver homogenates were measured by a solid-phase ELISA technique according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... sections were incubated overnight at 4°C with an antibody mix containing rat anti-mouse IL-18 (5 μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916 ...
-
bioRxiv - Cell Biology 2021Quote: ... and diluted to equal concentrations before performing an enzyme-linked immunoabsorbent assay (ELISA) using a Mouse/Rat FGF21 Quantikine ELISA kit (R&D Systems, Minneapolis, MN)(57).
-
bioRxiv - Neuroscience 2019Quote: ... IL-6 concentrations in maternal blood plasma (3 hours post-injection) were determined using a rat-specific ELISA DuoSet (R&D Systems, Abingdon, UK) as reported elsewhere [27] ...
-
bioRxiv - Cell Biology 2020Quote: ... Retinal crossections (12 µm) were processed for immunostaining using the following antibodies: rat monoclonal to CD45 antibody (clone 30-F11, R&D Systems, 1:50). Sections were pre-incubated with 5% goat serum (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: Cortical neurons were treated with either 1.5 µM Aβ or rat recombinant TIMP-1 peptide (R&D systems, Cat# 580-RT-010, Minneapolis, USA) at 100 ng/ml32 or a combination of both for 16 h as shown in figures.
-
bioRxiv - Neuroscience 2021Quote: Serum FGF21 protein levels of FGF21-Tg and WT C57BL/6J littermates were determined using a mouse/rat solid-phase FGF21 Quantikine ELISA Kit MF2100 (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... cell-free supernatants were collected and used to evaluate the concentrations of TGF-β1 with human and rat TGF-β1 ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2020Quote: ... Endothelial cells were seeded at a density of 2×105 per well on the insert coated with Cultrex rat collagen I (R&D Systems, Minneapolis, Minnesota, USA), and the insert was placed in the above-mentioned six-well plates ...
-
bioRxiv - Cell Biology 2022Quote: Intracellular cAMP expression levels were measured after 10min to 48h of PTH treatment using Mouse/Rat cAMP Assay Parameter™ Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions in both pre-differentiation COBs and BMSCs ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cytochrome c concentrations in the isolated cytosolic fraction were measured by rat/mouse cytochrome c quantikine ELISA Kit (MCTC0, R&D Systems, Inc., Minneapolis, MN).
-
bioRxiv - Neuroscience 2021Quote: ... the IGF-1 concentration in the medium was measured using the Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA)
-
bioRxiv - Cell Biology 2022Quote: The cDNA encoding the extracellular N-terminal IgV domain of human CD47 (residues 19-141) was amplified from the plasmid hCD47 VersaClone cDNA (RDC1523, R&D systems) and was cloned in the expression vector pGEX-4T-1 (GE Healthcare) ...