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Citations for R&D Systems :
401 - 450 of 1819 citations for Platelet activating factor receptor PTAFR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Spain) and supplemented with each one of the following combinations of growth factors: (i) 100 ng/ml Act A (R&D Systems, UK), (ii ...
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bioRxiv - Cancer Biology 2022Quote: ... culture medium was supplemented with 0.1 to 0.5 % BME (Cultrex Reduced Growth Factor Basement Membrane Extract, type 2, Pathclear, R&D Systems, 3533-005-02). We observed batch-to-batch variations of this product ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... containing 10% fetal bovine serum and 0.5% gentamicin in the presence of 1000 IU/ml of human granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Minneapolis, MN) and 1000 IU/ml of interleukin 4 (IL-4 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were kept in enriched N-2 medium with 10 ng/ml of basic fibroblast growth factor (R&D systems, Minneapolis, MN, USA) added every 24 h and medium changed every alternate day to keep the cells in an undifferentiated and proliferative state ...
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bioRxiv - Molecular Biology 2022Quote: ... from C57BL/6 mice were differentiated from proliferating mouse bone marrow progenitors through induction with 20 ng/ml granulocyte macrophage colony stimulating factor (GM-CSF) (R&D Systems, USA) over 6–9 days as described [35] ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived-dendritic cells (moDC) were obtained by incubation of monocytes with 1 ng/mL of granulocytes-macrophages colony-stimulating factor (GM-CSF, R&D Systems, France) and Interleukin-4 (R&D Systems ...
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bioRxiv - Microbiology 2023Quote: ... Harvested cells were cultured in RPIM 1640 medium containing 20% porcine serum and 10 ng/mL recombinant porcine granulocyte-macrophage colony-stimulating factor (GM-CSF, R&D Systems, UK) to differentiate mature bone marrow-derived macrophage (BMDMs ...
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bioRxiv - Neuroscience 2023Quote: ... ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2, Biolegend, San Diego, CA, USA / R&D Systems, Minneapolis, MN, USA), and forkhead box protein A2 (FOXA2 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Cancer Biology 2023Quote: ... the quantification of epidermal growth factor (EGF) protein levels in the serum was performed with the Mouse EGF Quantikine ELISA Kit (MEG00, R&D Systems Inc.) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2023Quote: ... and colonies were picked into a Cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 24-well plate for expansion ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were cultured in N2B27 with 10 ng/ml mouse basic fibroblast growth factor (bFGF) recombinant proteins (R&D systems #313FB025/CF). On Day 2 ...
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bioRxiv - Genetics 2023Quote: Generated hiPSC lines were layered onto a cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 6-well plate with cover slips (cat#12-542-BP FisherBrand ...
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bioRxiv - Genetics 2023Quote: Fully differentiated Day 30 Cardiomyocytes were resuspended onto a cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 6-well plate with cover slips (cat#12-542-BP FisherBrand ...
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bioRxiv - Neuroscience 2023Quote: ... A beveled glass needle was pre-loaded with mineral oil and the recombinant human IL-1 receptor inhibitor (IL-1RA, R&D Systems, Cat # 280-RA, resuspended in 0.1% BSA), then positioned in the injecting rig at a 90° angle relative to the horizontal plane of the Petri dish and head mold ...
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bioRxiv - Developmental Biology 2020Quote: Embryonic immortalised mouse epicardium-derived cells (mEPDC) were treated with 2.0 µg/ml human recombinant transforming growth factor (TGF)-β (R&D Systems; 240-B) for 30 hrs to induce EMT ...
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bioRxiv - Cell Biology 2019Quote: ... and expanded chondroprogenitor cells were re-suspended at 2.5 × 105 cells / mL in chondrogenic medium supplemented with 10 ng/ml human transforming growth factor beta 3 (TGF-β3; R&D Systems, Minneapolis, MN). Chondrogenic pellets were cultured at 37°C for 28 days ...
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bioRxiv - Neuroscience 2020Quote: ... L-glutamine and 10 ng/ml of carrier-free murine recombinant granulocyte and macrophage colony-stimulating factor (GM-CSF, 415-ML, R&D systems, Minneapolis, MN). Cell suspension from one brain was plated in a T75-cell culture flask coated with poly-D-lysine (P7280 ...
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bioRxiv - Cancer Biology 2020Quote: Twenty thousand HUVEC cells were seeded in reduced growth factor basement membrane extract (BME)-coated 96-well plate (R&D systems: 3470-096-K). Endothelial cells were exposed to 100 μl of the different benign or malignant cells conditioned media ...
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bioRxiv - Bioengineering 2020Quote: ... Microvascular fragment-containing gels were cultured in serum-free media supplemented with 10 ng/mL recombinant human vascular endothelial growth factor (rhVEGF; R&D Systems, Minneapolis, MN) (55) ...
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bioRxiv - Immunology 2021Quote: ... the bone marrow cells were culture in 6-well plates at density of 3×106 cells/mL in 3mL DC medium in the presence of mouse granulocyte macrophage-colony stimulating factor (GM-CSF; 1000 U/mL; R&D Systems, Minneapolis, MN) and interleukin 4 (IL-4 ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were serum starved overnight and then incubated with 10 ng/ml recombinant human transforming growth factor-beta 1 (TGF-β1) (R&D Systems, Minneapolis, MN) for indicated times.
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bioRxiv - Microbiology 2021Quote: ... from both tracheobronchial and alveolar infected ALI tissues and used to measure TH1/TH2 responses and growth factors with a customized 22-multiplex panel Human Magnetic bead Luminex assay (R&D Systems, MN, USA), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... were grown in their undifferentiated form on 20ug/mL Cultrex reduced growth factor basement membrane (catalog number 3433, R&D Systems, Minneapolis, Minnesota, USA) in DMEM/F12 (catalog number 11320033 ...
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bioRxiv - Cell Biology 2022Quote: ... and Connective Tissue Growth Factor (CTGF) (DY9190-05) were measured using Human DuoSet Enzyme linked immunosorbent assay (ELISA) kits (R&D Systems, Abingdon, UK) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and 50 mmol/L β-mercaptoethanol] supplemented with recombinant mouse stem cell factor (SCF) (12.5 ng/mL; R&D Systems, Cat. No. 455-MC) and recombinant mouse IL-3 (10 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 3 uM CHIR, 300 nM LDN193189 (LDN, Axon, Scottsdale, AZ) and 100 ng/mL epidermal growth factor (EGF, R&D systems, Minneapolis, MN) in Advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and 50 μg/ml L-Ascorbic Acid)) supplemented with Growth Factor Cocktail (5 ng/ml BMP4, 5 ng/ml bFGF, 10 ng/ml IL-6 (R&D systems, 206-IL), 10 ng/ml Flt3L (PeproTech ...
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bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: Protein profiling of the secreted factors from all cell cultures was performed using the Proteome Profiler Human XL Cytokine array kit (R&D systems, Minneapolis, MN) according to the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2021Quote: ... and SDF-1a), and other factors (PD-L1, PDGF-BB, S100B, GM-CSF, and VEGF-A) validated for NHP (R&D Systems, Minneapolis, MN, USA). Standard curves were generated using 5-parameter logistic regression using the xPONENT™ software provided with the MAGPIX instrument (Luminex ...
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bioRxiv - Microbiology 2020Quote: ... Primary BMDCs were isolated from C57BL/6 mice and cultured in Dulbecco’s modified Eagle medium for 3–5 days in the presence of 20 ng/mL recombinant granulocyte-macrophage colony-stimulating factor (R&D Systems, Minneapolis, MN, USA) as described previously (83) ...
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bioRxiv - Immunology 2021Quote: ... 50 ng/mL of recombinant human GM-CSF (Sanofi-aventis Cat# NAC2004–5843-01) and 2.5 ng/mL of tumor necrosis factor (TNF)-α (25 ng/mL; R&D Systems Cat# 210-TA). Next ...
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bioRxiv - Cell Biology 2022Quote: ... hMEC 3D acini were resuspended in 10 mg/ml Cultrex Reduced Growth Factor Basement Membrane Extract (BME) Type 2 (R&D Systems, #3532-005-02) and 25 μl BME domes were plated in each well of µ-Slides 8 (Ibidi) ...
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bioRxiv - Microbiology 2023Quote: ... organoids were mechanically disrupted into single cells and plated in droplets of Cultrex growth factor reduced BME type 2 (Biotechne | R&D systems 3533-010-02). For the 2D culture of airway organoids ...
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bioRxiv - Microbiology 2023Quote: ... Media was replaced with DMEM containing gentamicin and ciprofloxacin HCl alone or containing antibiotics and 10% NCS and/or 200 ng/mL Recombinant Human Insulin growth factor 1 (R&D Systems Cat#: 291-G1) for indicated minpost-treatment ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FBS and 1% penicillin/streptomycin with macrophage-colony stimulating factor (M-CSF, 30 ng/mL; 416-ML, R&D systems, Minneapolis, MN, USA) for 4 days to obtain BMMs ...
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bioRxiv - Cancer Biology 2023Quote: Transparent PET membrane culture inserts of 24-well plate (Falcon, 353097) were coated with Cultrex Reduced Growth Factor Basement Membrane Extract (BME) (R&D Systems, 3533-010-02) at 50μg per membrane (200μl of 0.25mg/ml BME stock per membrane ...
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bioRxiv - Bioengineering 2023Quote: RFP-MDA-MD-231 and GFP-HUVEC at 3 million/mL and 8 million/mL in precursor solution were photopatterned and perfused using the peristaltic pump previously mentioned at 0.2 rpm/mL for five days before tumor necrosis factor-alpha (TNF-α) (R&D Systems, Minneapolis, MN, USA) and Vascular endothelial growth factor (VEGF ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells were resuspended in 1 part Advanced DMEM/F12 and 2 parts Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems; 3533-010-02) for a final solution of 66% Cultrex ...
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bioRxiv - Immunology 2024Quote: ... were grown in DMEM supplemented with 10% FBS and 100 U/ml penicillin–streptomycin in the presence of macrophage colony-stimulating factor (M-CSF, 10 ng/ml, R&D Systems 416-ML-050), as described earlier in our studies ...
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bioRxiv - Neuroscience 2021Quote: ... Media was then exchanged to DMEM containing 15 ng/mL recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF; R&D Systems # 215-GM-010/CF). PBMC Mϕ were allowed to differentiate for an additional 5 days and were returned to DMEM 10% FBS until stimulation.
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bioRxiv - Cancer Biology 2019Quote: ... Human interleukin (IL)-8 and vascular endothelial growth factor (VEGF) were quantified in cell culture supernatants using DuoSet® ELISA (R&D Systems, Minneapolis, MN, USA), following manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: The presence of angiogenic factors in BEC CM was evaluated trough a Proteome Profiler Human Angiogenesis Array kit (ARY007, R&D Systems Inc., Minneapolis, MN, USA). For this ...
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bioRxiv - Immunology 2019Quote: We measured the concentrations of IL-1β and tumor necrosis factor alpha (TNF-α) in culture supernatants by using a commercial ELISA kit (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Molecular Biology 2023Quote: Crypts isolated from mouse intestine were seeded at a density of 250 crypts per 25 µL Matrigel (Cultrex reduced growth factor basement membrane extract, type R1, R&D Systems, Catalog #3433-010-R1) in 48 well plates ...
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bioRxiv - Bioengineering 2023Quote: ... Organoid basal growth media was supplemented with epidermal growth factor (EGF) (100 ng/mL, 10 ng/mL, 1 ng/mL R&D Systems Cat#236-EG-01M) or Epiregulin (EREG ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used were: Lrig1 antibody (R&D Systems, Cat. AF3688), ITGA6 antibody (clone GoH3 ...
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bioRxiv - Biochemistry 2019Quote: ... The following antibodies were used: anti Nephrin antibody (R&D Systems), anti-HAI-1 antibody (R&D Systems) ...