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Citations for R&D Systems :
101 - 150 of 177 citations for PRL Sheep HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The pre-immobilized beads were then incubated with 50 nM recombinant mouse SorCS1 ectodomain tagged with a C-terminal 6-His tag (SorCS1-His, Cat# 4395-SR-050, R&D systems) in ECS for 2 h at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... and His-p53 was obtained from R&D system.
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bioRxiv - Neuroscience 2023Quote: ... Either 1µL 500nM recombinant His-USP7 (R&D system) or 1µL deubiquitination buffer as vehicle control was added to each reaction and incubated for 1 hour at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... the same protein was labeled using its His-tag by incubating the antigen with an Alexa Fluor 647-conjugated anti-His-antibody (R&D Systems, IC0501R) for 30 minutes at room temperature at a 2:1 ratio (RBD molecules:IgG molecules) ...
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bioRxiv - Molecular Biology 2022Quote: ... Human tissues were stained with a sheep polyclonal antibody to integrin α11 (R&D Systems) and murine tissues with a rabbit polyclonal antibody to mouse α11 generated and kindly provided by Donald Gullberg (Department of Biomedicine ...
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bioRxiv - Immunology 2019Quote: ... The sheep anti-CD38 polyclonal antibody (R&D Systems, Cat# AF4947, diluted at 1:100) and rabbit anti-F4/80 monoclonal antibody (Cell Signaling ...
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bioRxiv - Biochemistry 2020Quote: ... His-MBP-PINK1 (R&D Systems, Cat. # AP-180-100) concentration was set to 50 ng/µl ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant His-tagged NSP12 (R&D Systems, catalog # 10686-CV) was incubated with in vitro transcribed and biotinylated RNA in 20 mM Tris ...
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bioRxiv - Bioengineering 2024Quote: ... His-tagged mouse TfR (TfR cross-reactivity, R&D Systems 9706-TR-050 ...
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bioRxiv - Developmental Biology 2021Quote: ... Unfixed cryopreserved embryos were sectioned and stained with sheep-anti-Prdm16 antibody (AF6295, R&D Systems) and FITC-conjugated anti-αSMA (F3777 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used for immunoblotting were sheep anti-DRAXIN (AF6149, R&D systems, 1 ug/mL), goat anti-β-ACTIN (AB0145-200 ...
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bioRxiv - Cancer Biology 2021Quote: ... and then stained with phycoerythrin (PE)-conjugated anti-sheep secondary antibody IgG (F0126, R&D systems). Cell sorting was conducted on a BD FACSAria IIu cell sorter (Franklin Lakes ...
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bioRxiv - Cell Biology 2020Quote: ... sheep polyclonal anti-Plexin-B2 (for Western Blot; 1:500, R&D Systems, cat. no. AF6836), rabbit monoclonal anti-E-cadherin (1:200 ...
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bioRxiv - Immunology 2020Quote: ... grids were incubated with sheep anti-STING antibody at 1:7 dilution (AF6516, R&D Systems), rinsed in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Sheep anti-RAMP1, -RAMP2, and - RAMP3 (AF6428, AF6427, and AF4875, respectively) were from R&D Systems. Mouse IgG antibody (PMP01X ...
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bioRxiv - Immunology 2021Quote: ... biotinylated ACE2-hFc or mouse anti-His mAb (R&D systems). Spike trimer (1 µM binding sites ...
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bioRxiv - Cancer Biology 2023Quote: ... The ligands used were His-Tagged human CD38 (R&D systems, catalog # 2404-AC ...
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bioRxiv - Neuroscience 2020Quote: ... To investigate the specificity of the sheep polyclonal antibody for plexin-B3 (R&D systems, Minneapolis, MN), the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp ...
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bioRxiv - Immunology 2020Quote: ... Following antibodies were used for UMOD Western blot: sheep anti mouse UMOD (R&D Systems, 1:1000), rabbit anti sheep IgG (Southern Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... as capture antibody and a sulfo-tagged detection antibody (sheep anti-mouse Trem2, AF1729 R&D Systems). MSD plates were developed using 2x MSD read buffer T ...
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bioRxiv - Pathology 2024Quote: ... Cells were then immunolabeled for 4hr at 4°C with sheep anti-CD31 (R&D Systems, AF806) + rabbit anti-cTNT (Abcam ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... 10 μg/mL anti-His tag antibody (clone AD1.1.10, R&D Systems), 50 ng/mL CpG ODN 2006 (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... 10 µg/mL anti-His tag antibody (clone AD1.1.10, R&D Systems), 50 ng/mL CpG ODN 2006 (Invivogen) ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged ALS protein (R&D Systems, 10 ng/well, 1.5 µM) was immobilized in wells via neutravidin and the nickel-charged-NTA-decorated iBodies® (IOCB Tech ...
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bioRxiv - Neuroscience 2023Quote: ... The plates were incubated with 0.25 μg/ml biotinylated sheep anti-mouse TREM2 antibody (R&D Systems, BAF1729) for 1 hr at RT with shaking ...
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bioRxiv - Bioengineering 2023Quote: ... 96-well plates were coated with anti-mouse protein C polyclonal sheep IgG (R&D Systems, Minneapolis, MN) and incubated at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.5 µm sections from TMAs or whole blocks were used for ARX (1:1500, R&D Systems, sheep, AF7068), PDX1 (1:100 ...
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bioRxiv - Physiology 2021Quote: ... The membranes were incubated with a sheep polyclonal antibody to human DG (AF6868; R&D Systems, 1:100 dilution) and a mouse monoclonal antibody to a glycoepitope on the sugar chain of α-DG (IIH6 ...
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bioRxiv - Pathology 2020Quote: ... the membranes were incubated with the sheep anti-human collagen I alpha 1 antibody (1:2000, R&D systems) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The primary antibodies used in this study were sheep anti-nephrin (#AF4269-SP, R&D systems, Abingdon, United Kingdom) diluted 1:300 ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human His-tagged CD80 (R&D Systems 9050-B1, Minneapolis, MN, USA) or CD86 (R&D Systems 9090-B2 ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with 100 ng Tie2‐Fc (with His tag, R&D systems) in TBST buffer at 4°C for 4 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant human TIM-4-HIS (R&D Systems, Cat# 9407-TM-050)) ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus ...
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bioRxiv - Cancer Biology 2021Quote: ... or 1h at 37°C with the sheep polyclonal anti-TWIST2 (1/200, AF6249, R&D Systems, Minneapolis, MN, USA) in Antibody Diluent Buffer from Ventana Medical Systems ...
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bioRxiv - Molecular Biology 2024Quote: ... The following antibodies were used for immuno-isolation of NRXN3 and controls: sheep polyclonal NRXN3 (cat. AF5269, R&D Systems), GFP (4745-1051 ...
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bioRxiv - Genetics 2024Quote: His Tag Alexa Fluor® 488-conjugated Antibody Catalog #: IC050G from R&D systems. 2019-nCoV RBD ...
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bioRxiv - Biophysics 2024Quote: ... Purified kinetochores were incubated with biotinylated α-His antibody (R&D Systems, Minneapolis MN) at an antibody concentration of 0.025 ng/µl for 1 hour at 4 °C with rotation ...
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bioRxiv - Immunology 2019Quote: ... then incubated overnight at 4°C in primary antibodies in 0.1% Triton X-100/PBS (rat anti-GP2, MBL, D278-3 at 1:500; sheep anti-SpiB, R&D Systems, AF7204 at 1:50 ...
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bioRxiv - Microbiology 2021Quote: ... serum was incubated for 2 h with polyclonal sheep IgG anti-LL-37 (2.5 – 20 μg ml−1; R&D Systems) or anti-hNP-1 (2.5 – 20 μg ml−1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems, catalog # IC0501R) or HER2-ED (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-mouse Lyve1 (1:500, #DP35135P, Acris Antibody GmbH, Herford, Germany) and sheep anti-mouse Trem2 (1:50, #AF1729, R&D Systems), and rabbit anti-mouse Ki67 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... plates were incubated for 1.5 hr at RT with biotinylated sheep anti-mouse TREM2 antibody (0.1 µg/ml, BAF1729, R&D Systems, RRID: AB_356109) diluted in BSA-PBST ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were re-suspended in 100 μl of FACS buffer and incubated with 10 μl of PE-conjugated anti-sheep IgG secondary antibody (F0126, R&D systems) for 30 min on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... separated and blotted onto 0.2 μm nitrocellulose as above and incubated overnight at 4°C in blocking buffer supplemented with 2 μg/ml polyclonal sheep IgG anti-LL-37 (R&D Systems). Membranes were washed in wash buffer (PBS with 0.05% Tween ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were then stained for 4 hrs with 10 µg mL-1 of donkey anti-sheep IgG NL493-conjugated antibody (NL012, R&D Systems) and 10 µg mL-1 donkey anti-rabbit IgG Alexa Fluor 555-conjugated antibody (ab150062 ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies: sheep anti-THP polyclonal antibody (1:40, catalog no. AF5175; R&D Systems), goat anti-MPO polyclonal antibody (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 5 µL (1:200) donkey anti-sheep IgG APC-conjugated antibody (R&D Systems, Cat. No. F0127) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal 6xHis tag produced in HEK293 cells and human recombinant PD-L1-Fc chimera protein produced in mouse NS0 cells were obtained from R&D Systems, Minneapolis ...