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Citations for R&D Systems :
51 - 99 of 99 citations for Modified Jis Dioxin & Furan Calibration Solution Cs0.2H Unlabeled 13C12 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: A solution of 10 ng interferon-β (IFN-β, R&D Systems, 8234-MB-010) diluted in PBS (2μl ...
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bioRxiv - Developmental Biology 2024Quote: ... 50 µl solution of FGF8 recombinant mouse protein (424-FC-025/CF, R&D systems) at a concentration of 1 µg/ml was dropped by a pipette on the ventral surface of the embryo.
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Sections were first incubated with an antigen retrieval solution (Antigen Retrieval Reagent-Basic, R&D Systems) at 80°C for 30 minutes followed by blocking (2% non-fat dry milk ...
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bioRxiv - Bioengineering 2021Quote: ... the slides were incubated in 5 μg/mL solution of anti-SOX2 antibodies (R&D Systems) or 5 μg/mL solution of anti-Sox17 (SantaCruz ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1% BSA-containing DBPS in Cultrex rat Collagen I solution (R&D Systems, 3440-005-01) 170 ul was loaded in the center chamber ...
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bioRxiv - Immunology 2021Quote: ... Cells were sequentially challenged by addition of the following agonist solutions: SLIGRL (100 μM; R&D Systems), then either rmIL-31 (300 nM ...
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bioRxiv - Developmental Biology 2022Quote: ... every 7 days Matrigel was lysed with Cultrex™ Organoid Harvesting Solution (R&D Systems™, MN), and isolated mGO ...
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bioRxiv - Developmental Biology 2023Quote: ... heating at 95°C for 5 min in antigen retrieval solution (CTS045, R&D Systems, Minneapolis, MN). After blocking non-specific binding in 2% non-fat milk in 0.1 M PBS containing 0.1% Triton X-100 (PBS/MT ...
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bioRxiv - Bioengineering 2023Quote: ... The lysed LVV solution was assessed using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems) as described previously76 ...
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bioRxiv - Molecular Biology 2021Quote: ... Additional reagents that were used are 2N Sulfuric Acid Stop Solution (Catalog no. DY994, R&D Systems, Minneapolis, MN), Triphenylphosphine (TPP ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of a solution containing 0.001 mg/mL of the detection biotinylated polyclonal antibody (BAF1559; R&D Systems) raised against NOTCH3 ECD and 2.5% BSA was added and was used for detection followed by agitation for 2h at RT ...
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bioRxiv - Biophysics 2023Quote: ... and then a binding buffer solution containing 200 nM of recombinant αVβ5 human integrin extracellular domains (R&D Systems) was slowly added to one of the wells of the fluid chamber ...
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bioRxiv - Immunology 2023Quote: ... Colonoids were passaged every 10-14 days by harvesting in Cultrex Organoid Harvesting Solution (R&D Systems, Minneapolis, MN) at 4°C with shaking for 30’ ...
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bioRxiv - Cancer Biology 2023Quote: ... in 2% BSA/PBS solution and the appropriate fluorescently-conjugated secondary antibody (R&D Systems catalog #NL006, 1:200). Coverslips were mounted onto slide with DAPI-containing ProLong Gold media ...
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bioRxiv - Immunology 2024Quote: ... and anti-B220 (Rat anti-mouse; monoclonal: RA3-6B2 clone; dilution 1:100 in blocking solution; R&D systems). Secondary antibody staining was performed for 50min at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... CHO-derived rhBMP2 standard and streptavidin-HRP working solution from the hBMP2 DuoSet ELISA system (DY355, R&D systems) were used for all experiments ...
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bioRxiv - Biophysics 2019Quote: ... Enzyme activity was confirmed in bulk solution using a fluorogenic substrate (Mca-PLAQAV-Dpa-RSSSR-NH2; R&D systems, ES003) 21 ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used overnight in 1% skimmed milk solution: anti-UCP1 (1:750, R&D Systems, MAB6158), anti-pCREB (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Drugs used for flow cytometry experiments were QVD-Oph (#OPH001, R&D Systems, 10 mmol/L stock solution in DMSO), S63-845 (Chemieteck ...
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag mouse monoclonal antibody (MAB050) and the basic-based antigen unmasking solution (CTS013) from R&D Systems (Minneapolis, MN); anti-FLAG M2 mouse monoclonal antibody (F1804) ...
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bioRxiv - Immunology 2021Quote: ... In-Cell ELISA was then developed using DY999 substrate solution according to the manufacturer’s recommendations (R&D Systems, Minneapolis, MN USA) and measured at 450 nm (and at 630 nm to assess the background ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were resuspended in an antibody solution containing 5μL DYKDDDDK Epitope Tag Alexa Fluor 647-conjugated Antibody (R&D Systems #IC8529R) and 45μL DBPS + 10% FBS and incubated in the dark at room temperature for one hour ...
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bioRxiv - Molecular Biology 2023Quote: ... were prepared to obtain solutions of TGF-β1 (10 ng/mL and 25 ng/mL, R&D Systems 240-B-002), palmitic acid (PA ...
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bioRxiv - Cancer Biology 2021Quote: ... one ml solution of 250 ng/ml type I collagen with or without 250 ng/ml POSTN (R&D Systems, Minneapolis, MN) was added to each well followed by one-hour incubation at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
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bioRxiv - Immunology 2019Quote: ... a suspension of 106 mononuclear cells in RPMI 1640 supplemented with 10% FCS was added to each insert in a well containing a solution of chemokine (R&D Systems). In some experiments ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked with a 10% Bovine Serum Albumin solution in PBS for 20 minutes and then incubated with the Alexafluor 647-conjugated antibody for Human ACE-2 (R&D Systems). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40; AF3075; R&D Systems) and rabbit polyclonal antibodies against GFAP (1:250 ...
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bioRxiv - Developmental Biology 2023Quote: E13.5 small intestinal tissues were harvested in Calcium/Magnesium free (CMF) PBS and washed several times in CMF PBS before being placed in 3mL of Cultrex Organoid Harvesting Solution (R&D Systems), a non-enzymatic basement membrane depolymerization solution ...
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bioRxiv - Cancer Biology 2023Quote: ... Culture media on the top of the matrix was removed and washed in cold PBS and the matrix was depolymerized with 200ul of Cultrex organoid harvesting solution (R&D systems) at 4°C for 2 h and then the isolated cell spheroids were lysed with 1% NP-40 cell lysis buffer containing protease and phosphatase inhibitor cocktail (Thermo-Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... cells from each well were harvested and resuspended in 100 µL of cell resuspension solution provided with human methylcellulose enriched media (R&D systems). Each cell suspension was added to 2.5 mL methylcellulose enriched media ...
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bioRxiv - Pathology 2023Quote: ... They were then first incubated with blocking solution for 1 hr and then with a mouse monoclonal anti-human CCR2 antibody (MAB150-SP; R&D Systems) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... all cells were spun down to remove the antibody solution and resuspended in fresh flow cytometry staining buffer (R&D Systems #FC001). Cells were then passed through round bottom Falcon tubes for flow cytometric analysis using a BD LSR Fortessa.
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bioRxiv - Developmental Biology 2024Quote: ... Organoids were retrieved from Cultrex by removing culture media and adding 500uL Cultrex Organoid Harvesting Solution (R&D Systems; 3700-100-01) to the wells of a 48 well plate ...
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bioRxiv - Bioengineering 2023Quote: The cerebral organoids in Matrigel were isolated from the pillar plate using Cultrex organoid harvesting solution (3700-100-01, R&D Systems) according to the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2024Quote: ... and treated with a blocking solution (dPBS +Ca2 +Mg2 with 2% (v/v) FBS) containing an anti-hTIM1(1:50-1:100, AF1750, R&D Systems) antibody ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels (n=3) were incubated for 4 hours in a solution of FBS-free DMEM supplemented with 10% Resazurin dye (AR002, R&D Systems) at 37°C ...
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bioRxiv - Bioengineering 2024Quote: The cerebral organoids in Matrigel were isolated from the pillar plate using Cultrex organoid harvesting solution (3700-100-01, R&D Systems) according to the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2019Quote: ... MSD GOLD 96-Well Streptavidin plates were blocked with 3% BSA and coated with a solution containing 0.25 µg/ml biotinylated polyclonal goat anti-human TREM2 capture antibody (BAF1828, R&D Systems, Minneapolis, MN). CSF and plasma samples diluted 1:4 in 1% BSA were added to the prepared plates and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... PBS was removed and replaced with 200 µL of blocking solution (dPBS with 2% (v/v) BSA) containing α-TIM1(1:100, AF1750, R&D Systems) or α - AXL antibody (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... in culture medium with recombinant mouse TNF-α (10 ng/ml from a stock solution of 100 μg/ml made up in 0.2 % bovine serum albumin and sterile PBS, R&D systems, aa-80325) to stimulate release of inflammatory mediators (TNF-FLS ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were fixed in 4% paraformaldehyde solution as previously described.39 Membranes were immunoblotted for AXL (AF154 R&D Systems at 1:500), MerTK (ab52968 ...
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bioRxiv - Cell Biology 2021Quote: ... or Recombinant Mouse Wnt-3a (10µg/ml stock solution in 0.1% BSA in PBS) (1324-WN-002, R&D systems, Bio-Techne, Minneapolis, MN) with DMSO and 0.1% BSA in PBS as vehicle controls ...
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bioRxiv - Microbiology 2021Quote: ... Domes were dislodged with a cell scraper in 1 mL of Cultrex Organoid Harvesting solution (Bio-techne, R&D Systems brand, 3700-100-01) and incubated for 1 hr at 4°C on a shaker at 250 rpm ...
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bioRxiv - Neuroscience 2022Quote: ... The cannula was connected to an osmotic minipump filled either with vehicle solution (PBS containing 0.1% BSA as a carrier) or 35 μg/ml of soluble TNF receptor-1 (sTNFR1, R&D Systems Inc. Minneapolis, MN).
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Immunology 2024Quote: ... Slides of aortic cross-sections were prepared and stained for elastin (Van Gieson’s Solution, catalog no. s289; Poly Scientific R&D Systems, Bay Shore, NY) smooth muscle actin (monoclonal anti-actin α-smooth muscle ...
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bioRxiv - Bioengineering 2024Quote: ... HLOs in Matrigel were either collected manually through pipetting in cold PBS-/- or isolated from Matrigel using Cultrex organoid harvesting solution (R&D Systems; 3700-100-01) according to the manufacturer’s recommended protocol ...
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bioRxiv - Bioengineering 2024Quote: HLOs in Matrigel were either collected manually by pipetting in cold PBS-/- or isolated from Matrigel using Cultrex organoid harvesting solution (R&D Systems; 3700-100-01) according to the manufacturer’s recommended protocol ...