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Citations for R&D Systems :
101 - 150 of 2323 citations for MMP 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... His-HBP was purchased from R&D Systems. Purified His-HBP was incubated with beads bound with GST (control) ...
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bioRxiv - Cell Biology 2023Quote: ... with 10% HI-FBS (R&D Systems S11150), 100x Pen/Strep (Gibco 15140-122) ...
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bioRxiv - Bioengineering 2019Quote: ... 2013] consisting of the osteogenic media supplemented with 100 ng/mL BMP-2 (recombinant human BMP-2, 355-BM-050, R&D Systems). Media was replaced every 3 days ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in the media (RPMI1640 +2% FBS) with human anti-Nectin-2 monoclonal antibody or isotype control (20 μg/mL, both R&D Systems) for 6h ...
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bioRxiv - Cell Biology 2020Quote: ... The beads were removed by centrifugation and the phosphorylated GST-NEPHRIN and GST-NEPH1 were mixed with recombinant purified active SHP-2 (Recombinant Human SHP-2; Cat. No.: 1894-SH; R&D Systems) in the presence of phosphatase buffer (10mM HEPES ...
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bioRxiv - Genetics 2023Quote: ... TGFB2 dosage in each eye (13 controls + 2 cases) was conducted with the Human TGF-beta 2 Quantikine ELISA kit (R&D Systems), following the provided instructions.
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bioRxiv - Neuroscience 2022Quote: ... and 2 ng/ml recombinant human NRG1-β 1 (#396-HB, R&D Systems, USA) as described in Poitelon et al ...
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bioRxiv - Molecular Biology 2022Quote: Human recombinant BMP-2 and BMP-4 were purchase from R&D Systems (Minneapolis, MN). Anti-Myc and anti-HA antibodies were purchased from Cell Signaling Technology (Danvers ...
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bioRxiv - Immunology 2020Quote: ... isolated NK cells were cultured in 1000 IU/mL human IL-2 (R&D Systems) for 24 hours ...
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bioRxiv - Physiology 2020Quote: ... with 2 µg of recombinant human TSG-6 (rTSG-6, R&D Systems, Minneapolis, MN) in 100 µl PBS (or with PBS alone ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 uM SB431542 (SA) and 10 ng/mL recombinant human FGF-2 (R&D Systems) were added on D2 for 2 days ...
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bioRxiv - Systems Biology 2023Quote: ... Tube 2: rat monoclonal anti-human GSK-3B(Clone 272536, catalog # MAB2506, R&D Systems) and rabbit polyclonal anti-human Phospho-GSK-3BCatalog # AF1590 ...
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bioRxiv - Immunology 2023Quote: ... were coated with human ACE-2 Fc chimera protein (R&D Systems cat# 10544-ZN) in cold PBS (100 μl per well ...
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bioRxiv - Microbiology 2023Quote: PLY-treated H292 monolayers or PMNs in suspension were treated with 1U PLY and incubated for 30 min at 37 °C with 10 μM fluorogenic peptide MMP substrate (Mca-PLAQAV-Dpa-RSSSR-NH2, R&D Systems) diluted in 25 mM Tris buffer ...
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bioRxiv - Developmental Biology 2024Quote: The MMP9 catalytic domains were incubated in a 96-well plate with different concentrations of Mca-PLGL-Dpa-AR-NH2 Fluorogenic MMP Substrate (ES001, R&D SYSTEMS), a substrate that is cleaved by the MMP9 ...
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bioRxiv - Microbiology 2019Quote: ... BSA (24 - 48 h at 4°C in rotation) for HIS-tagged rPtxS1 and rGαi proteins with mouse monoclonal anti-HIS (1:1000) (R&D Systems, MAB050), for Gαi with mouse monoclonal anti-Gαi (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... The pre-immobilized beads were then incubated with 50 nM recombinant mouse SorCS1 ectodomain tagged with a C-terminal 6-His tag (SorCS1-His, Cat# 4395-SR-050, R&D systems) in ECS for 2 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... cells were pelleted and resuspended in 100 µL FACS buffer (2% FBS in DPBS) containing 0.2 µg goat-α-human Ace-2 (R&D Systems #AF933). After 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems; R15-100). R15-100 was added every 2 to 3 days thereafter ...
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bioRxiv - Cancer Biology 2022Quote: ... and His-p53 was obtained from R&D system.
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bioRxiv - Neuroscience 2023Quote: ... Either 1µL 500nM recombinant His-USP7 (R&D system) or 1µL deubiquitination buffer as vehicle control was added to each reaction and incubated for 1 hour at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... TF-I cells medium contained 2 ng/ml of recombinant human GM-CSF (R&D Systems). Cells have been cultured for no longer than 4 weeks after thawing and were regularly tested for Mycoplasma contamination using PCR technique and were confirmed to be negative.
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bioRxiv - Cell Biology 2022Quote: ... cells were exposed for 2 days to human Activin A (8 ng/ml, R&D Systems) and human bone morphogenetic protein 4 (hBMP4 ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant human SUMO-1 and SUMO-2 were purchased from R&D systems (Cat. # K-700). Recombinant human SUMO activating enzyme E1 (SAE1/UBA2 ...
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bioRxiv - Microbiology 2021Quote: ... and soluble receptor interleukin 2 (sCD25) were analysed using the Human Quantikine Immunoassay (R&D Systems). All following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... and human fibroblast growth factor 2 (hFGF2) (20 ng/mL, R&D systems 233-FB-025) freshly added into the media ...
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bioRxiv - Immunology 2023Quote: ... CTL medium containing 100 IU/ml human IL-2 (R&D Systems, 202-IL-010/CF) was added to the plates and the expansion of T cells continued ...
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bioRxiv - Immunology 2021Quote: ... the same protein was labeled using its His-tag by incubating the antigen with an Alexa Fluor 647-conjugated anti-His-antibody (R&D Systems, IC0501R) for 30 minutes at room temperature at a 2:1 ratio (RBD molecules:IgG molecules) ...
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bioRxiv - Molecular Biology 2022Quote: ... Dickkopf (Dkk)-1 and matrix metalloproteinase (MMP)-12 were assayed using the multiplex Mouse Magnetic Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... His-MBP-PINK1 (R&D Systems, Cat. # AP-180-100) concentration was set to 50 ng/µl ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant His-tagged NSP12 (R&D Systems, catalog # 10686-CV) was incubated with in vitro transcribed and biotinylated RNA in 20 mM Tris ...
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bioRxiv - Bioengineering 2024Quote: ... His-tagged mouse TfR (TfR cross-reactivity, R&D Systems 9706-TR-050 ...
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bioRxiv - Microbiology 2020Quote: ... The transduced cells were stained with anti-human ACE-2 polyclonal goat IgG (AF933, R&D Systems) primary antibody at 1 ug/mL and donkey anti-goat IgG conjugated to Alexa Fluor 488 (ab150129 ...
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bioRxiv - Cell Biology 2019Quote: ... the substrates were incubated for 2 h with human E-cadherin-Fc (R&D system, Minneapolis, MN) at 1 μg / cm2 ...
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bioRxiv - Immunology 2022Quote: ... 1% PS and 100 IU/ml of human recombinant IL-2 (R&D Systems, 202-IL-050). Cells were used at low passage numbers ...
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bioRxiv - Immunology 2022Quote: ... Recombinant Human IL-27 Ra/WSX-1/TCCR Fc Chimera Protein (2 μg/ml, R&D Systems), Ultrapure LPS from E ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... plates were incubated for 2 h at RT with 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) diluted in PBSTBA ...
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bioRxiv - Immunology 2023Quote: ... cell culture supernatants or sulcus fluid from patients with different disease stage were evaluated for MMP-9 content using ELISA Duo Set (DY911, R&D System, Wiesbaden, Germany.) following the manufactureŕs protocol.
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... or recombinant human cathepsin L (CatL, 2 ng/ul, pH 5.5, 37°C for 1h; R&D Systems) as described previously (5) ...
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bioRxiv - Neuroscience 2022Quote: ... Human TDP-43 DuoSET ELISA kit DuoSet Ancillary Reagent Kit 2 (#DY008, R&D Systems, Minneapolis, MN, USA), and tetraspanin exosome marker CD81 (CSB-EL004960HU ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were stimulated with 2 ng/mL recombinant human TGF-β1 (R&D Systems, cat. # 240-B) diluted in starvation medium for varying durations of 30 ...
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bioRxiv - Immunology 2021Quote: ... biotinylated ACE2-hFc or mouse anti-His mAb (R&D systems). Spike trimer (1 µM binding sites ...
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bioRxiv - Immunology 2023Quote: ... Subsequently the cells were rested and expanded in kool aid complete media for 2 days supplemented with 100 U/mL recombinant Human IL-2 (R&D Systems Cat# 202-IL-010/CF). Subsequently ...
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bioRxiv - Molecular Biology 2020Quote: ... FGF-2 in the CM was assayed using a human FGF basic DuoSet ELISA Development Kit (R&D Systems) according to the manufacturer’s procedure.
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bioRxiv - Neuroscience 2019Quote: ... Human IFNγ and IL10 were quantified in culture supernatants (1:2 dilution) using DuoSet ELISA kits (R&D Systems). DuoSet ELISA Ancillary Reagent Kit 2 was used in conjunction with all DuoSet ELISA kits ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 5000 RU of Recombinant Human Desmoglein-2 Fc Chimera Protein (R&D Systems, Catalogue number 947-DM-100) was amine coupled to a CM5 sensor chip at a slow flow-rate of 10 μl/min to ensure uniform distribution on the chip surface.
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bioRxiv - Immunology 2022Quote: ... T cells then were washed and further incubated with recombinant human IL-2 (10 ng/ml; R&D systems) for up to 2 weeks at 37°C incubator with medium change every 3 days ...
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bioRxiv - Bioengineering 2023Quote: ... and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA) were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Microbiology 2023Quote: ... 5 fmol or 10 fmol SpyCEP were incubated with 2 pmol human CXCL8 and CXCL1 respectively (R&D Systems), in a final volume of 100 μl and incubated at room temperature for 30 minutes ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... 10 μg/mL anti-His tag antibody (clone AD1.1.10, R&D Systems), 50 ng/mL CpG ODN 2006 (Invivogen ...