Labshake search
Citations for R&D Systems :
101 - 150 of 1981 citations for M CSF Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... with 20 ng/mL human recombinant Oncostatin M (R&D Systems). On differentiation day 25 ...
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bioRxiv - Microbiology 2021Quote: ... and CD14+ monocytes were differentiated into macrophages (MDMs) using Macrophage-colony stimulatory factor (M-CSF) (R&D systems, Minneapolis, MN, USA) as previously described [20] ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC).
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bioRxiv - Cancer Biology 2020Quote: ... Macrophage differentiation from bone marrow cells was obtained by culture in standard growth medium supplemented with m-CSF (R&D Systems) at 30 ng/ml for 7 days.
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bioRxiv - Immunology 2020Quote: ... and RPMI-1640 supplemented as described and with additional 20 mM Hepes and 25 ng/ml M-CSF (R&D Systems). Medium was changed every third day and the cells were allowed to differentiate for 10 days ...
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bioRxiv - Immunology 2021Quote: ... CD14+HLA-DRneg/low/CD14+HLA-DRhigh monocytes were cultured in 96 well plates (200μL/well) in the presence of 20 ng/mL M-CSF (216-MC-005; R&D Systems) for 4 days.6 Monocyte-derived macrophages [(Mb) ...
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bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... with or without 1.25 µg/mL Scl-Ab or 500 ng/mL anti-M-CSF antibody (#AF416 from R&D Systems). After 8 days ...
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bioRxiv - Cell Biology 2021Quote: ... 200 U/ml recombinant murine GM-CSF and 10 ng/ml recombinant human TGFβ (R&D Systems, USA). Partial medium change was performed at day 3 of differentiation ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in osteoclast medium supplemented with 10 ng/ml of M-CSF and 5 ng/ml of murine RANKL (R&D Systems) for 7-10 days ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
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bioRxiv - Microbiology 2020Quote: ... Fully-differentiated MDM were generated by culturing MC under adherent conditions for seven days using RPMI-AS medium supplemented with 10 ng/mL M-CSF (R&D Systems). T cells were purified from PBMC using T cell pan isolation kit (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... and seeded in RPMI 1640 supplemented with 10% serum and 10 ng/ml recombinant macrophage colony-stimulating factor (M-CSF; R&D Systems) for 3 days ...
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bioRxiv - Immunology 2019Quote: ... from wild type and Irak1−/− mice were prepared by differentiation for 6 days in the same culture medium containing macrophage colony-stimulating factor (M-CSF; 60 ng/mL, R&D Systems). LPS was from Alexis Biochemicals (Salmonella minnesota R595 TLR grade ...
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bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...
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bioRxiv - Genetics 2020Quote: ... femurs and tibias were harvested and bone-marrow cells were obtained by flushing bones and differentiated for 7 days in DMEM media supplemented with 50 mg ml-1 recombinant macrophage-colony stimulating factor (M-CSF, R&D systems), 20% heat-inactivated FBS ...
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bioRxiv - Immunology 2022Quote: ... The differentiation to macrophages was initiated at day 0 by the addition of 20 ng/mL macrophage colony-stimulating factor (M-CSF, R&D Systems). Cells were cultured afterwards at 37°C and 5% CO2 for 7 days including the replacement of supplemented DMEM and replenishment of M-CSF at days 1 and 4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Osteoclast differentiation media consisted of standard OCY454 media supplemented with 25ng/ml M-CSF (R&D Systems Catelog # 416-TEC; Lot # ME5021121) and 5 ng/ml RANKL (R&D Systems Catalog #462-TEC-010 ...
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bioRxiv - Molecular Biology 2023Quote: ... over the course of 7 additional days with 25 ng/mL M-CSF and 25 ng/mL RANKL (both from R&D Systems), as previously described [17,30,49] ...
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bioRxiv - Microbiology 2024Quote: ... and were differentiated into monocyte-derived macrophages (MDMs) by stimulation with 20 ng/mL macrophage colony-stimulating factor (M-CSF) (R&D Systems). MDMs were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Molecular Biology 2022Quote: ... human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF, 50 ng/mL R&D Systems, 7954-GM-020/CF), and interleukin-4 (IL-4 ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 20 ng/ml of recombinant murine GM-CSF (rmGM-CSF, R&D systems). Plates were incubated in humidified atmosphere at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in complete αMEM with 40 ng/ml M-CSF (ProSpec-Tany TechnoGene, Rehovat, Israel) and l0 ng/ml RANKL (R&D Systems, MN). Tartrate-resistant acid phosphatase (TRAcP ...
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bioRxiv - Developmental Biology 2019Quote: ... and 100 ng/ml CHO-derived mouse R-spondin 1 or 5 ng/ml CHO-derived mouse R-spondin 2 (R&D System). The final concentration of Rspondins in the culture medium was initially tested in pilot experiments in order to add comparable amounts of bioactive ligands (bioactivity measured by TOPflash assays and organoid growth curves ...
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bioRxiv - Physiology 2021Quote: ... primary osteoclast precursors were seeded after initial culture on non-treated plates into 6 well plates in the presence of 30ng/mL recombinant mouse M-CSF (R&D systems, Minneapolis, MN) for 3 days ...
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bioRxiv - Neuroscience 2021Quote: Differentiated BMDM were plated in 96-well plate at density of 30,000 cells/well either in the presence of 40ng/ml M-CSF (R&D systems, 416-ML-050) or without M-CSF ...
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bioRxiv - Cell Biology 2023Quote: ... non-adherent hematopoietic progenitors were pooled and plated at 7x104 cell/well (96-well) with complete media supplemented with 30 ng/mL M-CSF (R&D Systems, 416-ML). At 90% confluence ...
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bioRxiv - Microbiology 2024Quote: ... Monocytes were differentiated into hMDMs for at least 7 days in complete medium containing 10 ng.mL-1 of recombinant human macrophage colony-stimulating factor (rhM-CSF; R&D systems).
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bioRxiv - Microbiology 2023Quote: ... supplemented with 20 ng/mL of recombinant human oncostatin M (OSM, R&D systems). For the co-culture experiment ...
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bioRxiv - Immunology 2020Quote: ... Cells were isolated by flow cytometric cell sorting on a BD FACSMelody and cultured in StemPro-34 supplemented with either M-CSF or VEGF (R&D Systems, cat #293-VE).
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bioRxiv - Cell Biology 2022Quote: ... BMMs were then seeded at 1 × 106 cells/well in 24-well plates and stimulated with 30 ng/mL RANKL and 30 ng/mL M-CSF (R&D Systems, Minneapolis, MN, USA) for 5 days to generate mature OCs ...
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bioRxiv - Cancer Biology 2023Quote: ... BMSCs were plated in T-75 flasks overnight with standard OCY454 media supplemented with 10ng/ml M-CSF (R&D Systems Catalog #416-ML-010) at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FBS and 1% penicillin/streptomycin with macrophage-colony stimulating factor (M-CSF, 30 ng/mL; 416-ML, R&D systems, Minneapolis, MN, USA) for 4 days to obtain BMMs ...
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bioRxiv - Cell Biology 2023Quote: ... osteoclast differentiation was initiated using αMEM complete media supplemented with 30 ng/mL M-CSF and 100 ng/mL RANKL (R&D Systems, 462-TEC/CF)(57) ...
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bioRxiv - Immunology 2024Quote: ... were grown in DMEM supplemented with 10% FBS and 100 U/ml penicillin–streptomycin in the presence of macrophage colony-stimulating factor (M-CSF, 10 ng/ml, R&D Systems 416-ML-050), as described earlier in our studies ...
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bioRxiv - Immunology 2021Quote: ... G-CSF ELISA kit (R&D Systems), IL-6 mouse ELISA kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... GM-CSF (R&D systems, Minneapolis, MN), and MIP1α (R&D systems ...
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bioRxiv - Cell Biology 2024Quote: ... G-CSF and CXCL12 (R&D Systems).
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bioRxiv - Neuroscience 2021Quote: ... Protein expression for the expressed human LTα transgene was measured in snap-frozen rat CSF using a human TNF-beta/TNFSF1 Quantikine ELISA kit (DTB00, R&D systems) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... anti-hCD14 magnetic beads were used and isolated cells were differentiated to human monocyte-derived macrophages (hMDMs) by addition of 50 ng/ml rhM-CSF (R&D Systems) to XVivo medium (Lonza) ...
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bioRxiv - Immunology 2023Quote: ... the non-adherent fraction was seeded at 106 cells/well in 24-well plates in medium containing 30ng/ml M-CSF and 10ng/ml RANKL (R&D Systems, Cat. 452-TEC-019). The medium was replaced with supplemented M-CSF and RANKL after 48hr ...
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bioRxiv - Cell Biology 2023Quote: ... after which the medium was changed to α-MEM containing 20 ng/ml M-CSF and 20 ng/ml RANKL (462-TEC; R&D Systems, Minneapolis, MN, USA). The differentiation medium was changed every 24 hr during the 5-day differentiation period.
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bioRxiv - Developmental Biology 2024Quote: ... and 100 ng/mL CHO-derived R-spondin1 (R&D System)] ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... containing 10% fetal bovine serum and 0.5% gentamicin in the presence of 1000 IU/ml of human granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Minneapolis, MN) and 1000 IU/ml of interleukin 4 (IL-4 ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng/mL GM-CSF (R&D Systems) and 10 ng/mL IL-4 (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... and 20ng/ml GM-CSF (R&D System) at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... G-CSF ELISA kit (R&D Systems, MCS00); Corticosterone ELISA (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... GM-CSF (1000 U/µl, R&D Systems), Staphylococcus-Enterotoxin-B (SEB ...
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bioRxiv - Immunology 2023Quote: ... G-CSF (5 ng/ml, R&D systems), GM-CSF (100 U/µl ...