Labshake search
Citations for R&D Systems :
401 - 450 of 450 citations for Lifeink 200 Collagen Bioink since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
PVT1, a YAP1 dependent stress responsive lncRNA drives ovarian cancer metastasis and chemoresistancebioRxiv - Cancer Biology 2022Quote: ... epithelial to mesenchymal transition (EMT) was induced with 200 pM of Human recombinant TGF-beta 1 (R&D Systems, 240-B); verteporfin (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... 15 μL of 2x concentrated ubiquitin-rhodamine 110 (Ub-Rho110; 200 nM, FAC 100 nM [R&D Systems, U-555-050]) in USP30 activity assay buffer was dispensed into the compound-rhUSP30 containing plate using the Dragonfly Discovery liquid dispenser and fluorescence read on the FLIPR TETRA plate reader (Molecular Devices) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Colony forming assays were performed by plating 200 cells of each isolated subpopulation in Complete Methylcellulose Media (R&D Systems; HSC003).
-
bioRxiv - Bioengineering 2024Quote: ... or KR5 medium[26,27] with 4.5 μM CHIR99021 (FUJIFILM Wako Pure Chemical Corporation) and 200 ng/mL bFGF (R&D Systems, Inc.), referred to as “KR5_Mat” ...
-
A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite AdipogensbioRxiv - Pharmacology and Toxicology 2021Quote: ... diluted 1:200 and analyzed for adiponectin by ELISA following the manufacturer’s instructions (Mouse Adiponectin/Acrp30 Quantikine ELISA Kit, R&D Systems, MRP300). Absorbance (450 nM ...
-
bioRxiv - Neuroscience 2021Quote: ... Each anti-VEGF-A injection consisted of 200 μL of 32.5 pM concentration of antibody against mouseVEGF-A164 protein (YU1418071, R&D Systems, Inc.) in saline ...
-
bioRxiv - Developmental Biology 2022Quote: ... Lung lobes were incubated with primary antibody (anti-VWF, rabbit, 1:100, F3520, Sigma and anti-EMCN, goat, 1:200, AF4666, R&D Systems), in 5% donkey serum and 0.5% Triton X-100 in phosphate buffered saline (PBST ...
-
bioRxiv - Cancer Biology 2021Quote: MDA231-LM2 cells in DMEM with 10% FBS were cultured with or without incremental dosages (10-200 ng/ml) of recombinant OPG (R&D Systems) for 30 min ...
-
bioRxiv - Immunology 2022Quote: ... 1:1000 diluted in PBS followed by a secondary fluorochrome anti-rabbit NorthernLights NL637-conjugated antibody (NL005; R&D systems, 1:200). Sections were then mounted with Fluormount G containing DAPI (Southern Biotech) ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were incubated in cSFDM (including B27 supplement with vitamin A) with 200 ng/ml rmWNT3A (R&D Systems, #1324-WN-010), 100 ng/ml rhFGF2 (R&D Systems ...
-
bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614], 25 ng/mL SHH-C25II [R&D Systems, 464-SH-025] ...
-
bioRxiv - Immunology 2023Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...
-
bioRxiv - Biochemistry 2023Quote: ... plates incubated with biotinylated antibody were washed and incubated at 37°C for 1 hr with a 1/200 dilution of Streptavidin-HRP (Catalog #890803, R&D systems). Plates were then washed and developed using 100□μl/well SureBlue TMB (3,3′ ...
-
bioRxiv - Immunology 2023Quote: ... SLPI was measured urine samples diluted 1:20-1:200 in sterile PBS + 1% BSA using the Human SLPI DuoSet ELISA kit (CAS: DY1274-05, R&D Systems) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... 10% FBS, 1% PS) while freshly prepared media (RPMI, 10%, FBS 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 mM sodium pyruvate, 1% PS), while freshly prepared media (RPMI, 10% FBS, 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
-
bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 5 µL (1:200) donkey anti-sheep IgG APC-conjugated antibody (R&D Systems, Cat. No. F0127) for 30 minutes on ice ...
-
bioRxiv - Developmental Biology 2024Quote: Cells in 60 wells were incubated with primary goat polyclonal anti-mouse E-cadherin (R&D Systems, Cat. No AF748, 1:200) in cold 5% FBS/PBS for 20 min on ice ...
-
bioRxiv - Cell Biology 2021Quote: ... For IFN pre-stimulation, 200 IU/ml IFN-β (IFN-β1, Bioferon, Laupheim, Germany) or IFN-γ (R&D Systems, Minneapolis, MN, USA) were added at the time of seeding ...
-
Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: For pro-fibrogenic mediator studies control lung fibroblasts were treated in the presence of EGF (R&D systems, 236-GMP-200, 10ng/mL), TGFβ1 (R&D systems ...
-
bioRxiv - Microbiology 2022Quote: ... PBS was removed and replaced with 200 µL of blocking solution (dPBS with 2% (v/v) BSA) containing α-TIM1(1:100, AF1750, R&D Systems) or α - AXL antibody (1:100 ...
-
bioRxiv - Neuroscience 2021Quote: ... Plates were washed 5 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of IL-1β conjugate (R&D Systems, UK). Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems ...
-
bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of detection antibody (R&D Systems, UK). Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP ...
-
bioRxiv - Genetics 2020Quote: ... Cells were incubated in 0.5% serum-containing media before treatment with 200 ng/mL recombinant Shh N-terminal peptide (R&D Systems, 1314-SH) diluted in dimethyl formamide (DMF).
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were then blocked with odyssey PBS for 1 hour at room temperature and incubated with the anti-hERBB2/Her2 AF488 antibody (1:200, R&D systems biotechne) overnight at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... Some sections were stained with antibodies to early endoderm (GATA6-CST 5851 1:1600) neurectoderm (Beta3 tubulin R&D Systems MAB1195 1:200) or smooth muscle actin in mesoderm (alpha SMA R&D Systems MAB1420 1:200 ...
-
bioRxiv - Genomics 2021Quote: ... a total of ∼200 million cells were harvested and stained for surface ACE2 abundance as previously described with ACE2 antibody (R&D Systems #MAB9332) at 1:50 dilution in FACS buffer (PBS supplemented with 2% FBS ...
-
bioRxiv - Cell Biology 2022Quote: ... primary BMSCs were plated in 6-well dishes until 80% confluent and serum starved (0.5% FBS) for 72 hours prior to treatment with one or a combination of CXCL12 (200 ng/ml, R&D Systems, 460-SD) and Wnt3a (100 ng/ml ...
-
bioRxiv - Cell Biology 2022Quote: ... primary BMSCs isolated from C57BL/6 mice were plated in 6-well dishes until 80% confluent and serum starved (0.5% FBS) for 48 hours prior to treatment with one or a combination of CXCL12 (200 ng/ml, R&D Systems, 460-SD), Wnt3a (100 ng/ml ...
-
bioRxiv - Neuroscience 2023Quote: ... Primary antibodies (DCX, Goat anti-doublecortin, 1:500, Santa Cruz; SOX2, Goat anti-sex determining region Y-box 2, 1:200, R&D Systems; CLR ...
-
bioRxiv - Immunology 2023Quote: ... slides were probed with primary antibodies (CD31 1:200, Biolegend, MEC13.3; mSPARCL1 1:500, R&D systems, #AF2836-SP; hSPARCL1 1:500, R&D systems, #AF2728-SP ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then sections were incubated overnight at 4 ◦C with goat polyclonal anti-S100a10 antibody (1:200; AF2377, R&D Systems, Minneapolis, MN, USA), rabbit polyclonal anti-5HT1B Receptor antibody (1:100 ...
-
bioRxiv - Immunology 2024Quote: ... 1x106 cells then stained in 200 µL on ice for 30 minutes with: Alexa Fluor® 405 anti-IFNα/β R1 (1µg, FAB245V, R&D Systems), PE anti-IFNα R2 (1µg ...
-
bioRxiv - Systems Biology 2021Quote: ... we added one or combinations of the following recombinant proteins to the medium: 200 ng/mL of recombinant mouse FGF4 (R&D Systems, #7486-F4), 200 ng/mL of recombinant human FGF5 (R&D Systems ...
-
bioRxiv - Genetics 2020Quote: ... culture medium was replaced with KSR medium (Chambers et al., 2009, 2011) supplemented with 200 ng/ml of recombinant human Noggin (R&D Systems, Minneapolis, USA) and 10 μM of SB431542 (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2021Quote: Multiplexed ELISA on interstitial fluid from matched T and NAT fresh tissues were performed on a Luminex 200 platform (Luminex Inc.,) using custom kits of pre-mixed antibody-coated beads (R&D System Inc., MN), which included the following analytes ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by incubation with anti-CD31 primary antibody (1:200, #550274, BD Biosciences, San Jose, CA, USA; #BBA7, R&D systems, Minneapolis, MN, USA) and Alexa Fluor 594-conjugated secondary antibody.
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then seeded on the coated flasks and supplemented with 100 ng/ml of human PDGF-BB re-suspended in 0.1%BSA/4mM HCl/PBS (R&D Systems; 200-BB-050) daily for at least 7 days ...
-
bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
-
bioRxiv - Microbiology 2023Quote: ... 1 x 107 cells were added to 100mm Petri dishes containing 15 ml of complete RPMI media plus 200 to 1000 U/ml IFN-β (R&D Systems 8234MB010). All cells were grown in a 37⁰C incubator with 7.5% CO2 for 5 h to 4 days (as indicated ...
-
bioRxiv - Bioengineering 2024Quote: ... Different concentrations of endogenous Cyr61 (0-200 ng/ml; Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) and TGFβ (0 or 10 ng/ml ...
-
bioRxiv - Developmental Biology 2020Quote: ... was coated with 200 µL 91 µg/mL Matrigel in PBS or 8 µg/mL E-cadherin extracellular domain (E-cad-ECD; R&D Systems, 8505-EC-050) in PBS for 1-3 hours at 37°C ...
-
bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
-
bioRxiv - Microbiology 2022Quote: ... 5×105 isolated splenocytes were cocultured with different stimuli in 200 μL of RPMI-10% FBS complete medium for 48 h on IFN-γ-coated plates (Cat# EL485, R&D systems, Minneapolis, USA). Fifteen-mer overlapping peptides from SARS-CoV-2 spike glycoprotein (Cat# PM-WCPV-S-1 ...
-
bioRxiv - Microbiology 2023Quote: ... Media was replaced with DMEM containing gentamicin and ciprofloxacin HCl alone or containing antibiotics and 10% NCS and/or 200 ng/mL Recombinant Human Insulin growth factor 1 (R&D Systems Cat#: 291-G1) for indicated minpost-treatment ...
-
bioRxiv - Biophysics 2023Quote: ... The reversible osmotic stress eliminates these wrinkles and allows the conversion of effective 3D protein concentrations into 2D receptor concentrations.(37) The swelling media was supplemented with 50 nM dimeric ephrinA1-Fc (R&D Systems, #602-A1-200). The cells were allowed to equilibrate for 10 minutes at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... 3-6 consecutive sections were prepared from each sample and immunostained with anti-Iba1 antibody (Wako; Richmond, VA; 1:3000) and anti-CD31 antibody (R&D Systems; Minneapolis, MN; 1:200). Images of the ventricular and subventricular zones at the level of the frontal cortex were acquired on Leica SP8 confocal microscope using a 40X (1.3NA ...
-
bioRxiv - Cell Biology 2023Quote: HUVECs cultured in plastic flasks were left untreated or were treated with hrLIGHT (50, 100, 200 ng/mL; R&D Systems Bio-techne, Minneapolis, MN, USA) diluted in 0.2 % BSA in PBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution). After incubation with primary antibody ...
-
bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution), followed by incubation with primary antibody ...