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Citations for R&D Systems :
601 - 650 of 4239 citations for Human Immunodeficiency Virus p24 Protein HIV 1 Clade B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Mouse IL-1β and TNFα and IL-6 in cell culture supernatants or serum were measured using Human IL-1 beta/IL-1F2 DuoSet ELISA (DY201, R&D Systems), Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401 ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Genetics 2021Quote: ... Cell culture supernatants were collected and assayed for the secretion of pro-Col1A1 using Human Pro-Collagen I alpha 1 DuoSet ELISA kit (DY6220-05, R&D Systems) as described by the manufacturer.
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bioRxiv - Immunology 2019Quote: ... and resuspended for culture at 37°C + 5% CO2 for 1 h prior to stimulation with recombinant human TNFα (R&D Systems). Following stimulation ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated for 16 h prior to infection with addition of 50 IU mL-1 human IFNγ (285-IF, R&D Systems). Induction of GBP expression in the Dox-inducible cells was performed with 200 ng mL-1 Dox overnight (D9891 ...
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bioRxiv - Bioengineering 2020Quote: Lambda interferon levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Biological activity experiments were performed using ...
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bioRxiv - Immunology 2021Quote: ... Cell supernatant was collected and 1 ml was used for Proteome Profiler™ Human Cytokine Array (ARY005B, R&D Systems, Inc., USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... cells were wash three times with PBS to remove any residual FBS and followed by 1-hour treatment with goat anti-human ACE2 antibody (R&D Systems) or anti-hDDP4 antibody (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... Calprotectin levels were determined in optimally diluted (1:20) extracts using Human-S100A8/S100A9 Heterodimer DuoSet-ELISA kit from R&D Systems. Zonulin levels were determined in similarly diluted extracts using human Zonulin Stool ELISA kit from ALPCO ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Cancer Biology 2021Quote: ... Fibroblasts+rhTGFβ1 medium was incubated with 1 μg/mL of anti-human HGF antibody or with the respective mouse IgG1 isotype control (both from R&D Systems) for 1 hour at 37ºC.
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bioRxiv - Cell Biology 2022Quote: The concentration of active TGF-β1 was measured using the Human /Mouse /Rat /Porcine /Canine TGF-beta 1 Quantikine ELISA kit (DB100B, R&D Systems). Briefly ...
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bioRxiv - Systems Biology 2019Quote: ... cells were washed with 1× Phosphate buffered saline (PBS) and stimulated with 1000 U/mL of human recombinant IFNβ (R&D Systems) in DMEM for the designated length of time ...
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bioRxiv - Immunology 2020Quote: ... Cells were re-seeded onto 24-well plates at 1 × 105 cells/well and polarized for 48 hr with the addition of 50 ng/ml human IFNγ (R&D systems) for M1 macrophages ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: ... Lambda IFN levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). IL-6 was measured by human IL-6 DuoSet ELISA (DY206 ...
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bioRxiv - Immunology 2022Quote: Investigations into the effect of SmCI-1 and MutSmCI-1 on cytokine production were examined using a Human XL Cytokine Luminex Performance Panel Premixed Kit (bio-techne/R&D systems). Human blood mononuclear cells (PBMCs ...
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... and stained for total and relevant phosphoproteins with the following antibodies in one of the three tubes: Tube 1: mouse monoclonal anti-human RPS6 (Clone 522731, catalog # MAB5436, R&D Systems) and rabbit polyclonal anti-human Phospho-RPS6 (Catalog # AF3918 ...
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bioRxiv - Pathology 2023Quote: ... They were then first incubated with blocking solution for 1 hr and then with a mouse monoclonal anti-human CCR2 antibody (MAB150-SP; R&D Systems) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: PD-L1 was detected in K562 or THP-1 EVs using the Human PD-L1 DuoSet ELISA kit and DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 400 µg of protein from mock- and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 30 minutes in 5% Bovine Serum Albumin and incubated with primary antibodies against MRC2 (1:1,200: anti-mouse: AF4789, 1μg/mL: anti-human: AF5770; R&D Systems, Bio-Techne), SEL1L (1:2,000 ...
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bioRxiv - Microbiology 2023Quote: ... Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA or Human CXCL10/IP-10 DuoSet ELISA reagents (biotechne R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... The amount of TGF-β1 present in the media was quantified using the Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA kit(R&D systems). The results were normalized to the total number of cells in the culture at the moment of media collection.
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the labeling of aforementioned transcription factors: Human T-bet/TBX21 Alexa Fluor 488-conjugated Antibody (1:20, IC53851G, R&D Systems), Gata-3 Monoclonal Antibody (TWAJ) ...
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bioRxiv - Cancer Biology 2023Quote: ... female BALB/c mice were anaesthetized and injected as described above with 1 μg CINC-1 protein (453-KC-050/CF, R&D systems, Abingdon, UK) in 1 μL saline in the left striatum ...
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bioRxiv - Cancer Biology 2021Quote: ... Phospho-kinase arrays: proteome profiler human phospho-kinase array and human phospho-RTK array kits including 49 RTKs were purchased from R&D Systems. Indicated SW620 cells were lysed ...
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bioRxiv - Neuroscience 2021Quote: The cytoplasmic fraction of human postmortem brain homogenates and astrocyte culture media were used to determine cytokine content using Mouse or Human Proteome Profiler arrays (Mouse Cytokine Array Panel A and Human Cytokine Array, R&D Systems), according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine profiling of these pooled supernatants was performed using a human cytokine array kit (human cytokine array kit, panel A; R&D Systems) and near-infrared fluorescence detection using IRDye 800CW Streptavidin (Li-Cor Biosciences).
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human ACE2 polyclonal antibody (AF933) and mouse anti-human ACE2 mAb MAB9332 were available from R&D Systems (Minneapolis, MA). Rabbit anti-SARS-CoV-2 RBD (40592-T62 ...
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bioRxiv - Systems Biology 2021Quote: ... dispersed human islet cells and iPSC-beta cells were exposed to human pro-inflammatory cytokines IL1β (50 U/mL; R&D Systems) and IFNγ (1,000 U/mL ...
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bioRxiv - Immunology 2020Quote: ... The expression of PD-L1 and IL-6 from bronchoalveolar lavages was quantitated following the protocols of ELISA kits: Human IL-6 Quantikine ELISA Kit and Human/Cynomolgus Monkey B7-H1/PD-L1 Quantikine ELISA Kit from R&D Systems.
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bioRxiv - Immunology 2022Quote: ... supplemented with recombinant human IL-4 (50 ng/mL) and recombinant human GM-CSF (100 ng/mL) (R&D Systems, Minneapolis, MN) with one additional supplement of fresh media and cytokines at day 3 of culture as previously described 12 ...
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bioRxiv - Immunology 2019Quote: ... Immature dendritic cells were prepared by treatment of monocytes with 500 U/ml human IL-4 and 800 U/ml human GM-CSF (R&D Systems) in CellGenix media for 6 days ...
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bioRxiv - Cancer Biology 2020Quote: Human S100A8/S100A9 heterodimer amounts in human plasma samples were quantified using S100A8/S100A9 heterodimer Quantikine ELISA kit (R&D System, DS8900) following the manufacture protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and IL11 in equal volumes of cell culture media collected from experiments with primary human cells were quantified using Human IL-8/CXCL8 Quantikine ELISA Kit (D8000C, R&D Systems), Human IL-6 Quantikine ELISA Kit (D6050 ...
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bioRxiv - Bioengineering 2023Quote: The relative cytokine content of the aged iCMs before and after ECM treatment and of the decellularized human heart ECM were obtained using the Human XL Cytokine Array Kit (ARY022B, R&D Systems). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Proteome Profiler Array Human XL Oncology Array (Catalog Number ARY026) and Human XL Cytokine Array (Catalog Number ARY022B) from R&D Systems, Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The culture supernatants were analyzed for cytokines using the Human TNF-α and Human IL-12 DuoSet ELISA kits (R&D Systems) following the manufacturer’s protocol.
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bioRxiv - Bioengineering 2020Quote: ... Mouse IL-28 A/B (IFN-lambda 2/3) Du-oSet ELISA (DY1789B, R&D Systems), was used to assess cross-reactivity.
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bioRxiv - Developmental Biology 2019Quote: ... or DMEM+10% FBS+10 ng/mL TGFβ1 (Cat. # 240-B, R&D Systems, Minneapolis, MN). A P200 tip was used scratch down the midline of every well ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with or without 10 ng/ml TGFβ (R&D Systems, Cat. # 240-B) for another 24 hr and harvested for RNA extraction after 72 hr.
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bioRxiv - Bioengineering 2023Quote: ... B cell activating medium was supplemented with 5 ug/mL anti-CD40 antibody (R&D systems) and 20 ng/mL IL-4 (R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: ... starved overnight and exposed to 1.5 ng/ml of TFGβ (#100-B-001, R&D Systems) for 6 and 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... stimulation with transforming growth factor β (TGF-β, 240-B, R&D Systems, Minneapolis, MN, USA), dissolved in 0.1% (weight/volume [w/v] ...
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bioRxiv - Cancer Biology 2021Quote: ... Human Phospho-RTK Array Kit (ARY001B, R&D Systems) was used according to the manufacturer’s instructions and the blots were imaged and quantified in the same way as western blots.
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bioRxiv - Bioengineering 2021Quote: ... and anti-human PDGFR-β (R&D Systems, AF385). After three 8-hour washes in PBST at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human ACE2 antibody (#AF933, R&D Systems) was serially diluted and applied to Huh 7.5.1 cells ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant human NOGGIN (100 ng/ml, R&D Systems), recombinant human EGF (50 ng/ml ...