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Citations for R&D Systems :
351 - 400 of 2076 citations for Hepatitis E Virus Capsid Protein ORF2 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and a fusion of mPrtg ECD (F36-A952) to a His6 tag (Biotechne R&D Systems 6795-PR) were used as baits ...
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bioRxiv - Bioengineering 2023Quote: ... purified FLAG tagged protein (recombinant p53 protein, R&D Systems) was run with vesicles to quantify loading ...
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bioRxiv - Systems Biology 2021Quote: ... we added one or combinations of the following recombinant proteins to the medium: 200 ng/mL of recombinant mouse FGF4 (R&D Systems, #7486-F4), 200 ng/mL of recombinant human FGF5 (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: The relative content of 111 cytokine proteins (Table S1) was determined using the dot blot based mouse XL cytokine array kit following manufacturer’s instructions (R&D Systems, Cat. No: ARY028). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Fractalkine protein levels were measured in hippocampal lysates via the R&D System’s Mouse Fractalkine (CX3CL1) Quantikine ELISA Kit (R&D Systems cat. Nr. MCX310) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Recombinant human DLL4 (DLL4 fused with the Fc domain of human IgG; R&D Systems; 1506-D4) was resuspended to a final concentration of 10 μg/ml in sterile PBS containing 1% wt/vol bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: Co-immunoprecipitation experiments using the ECD of human TrkB-Fc (Cys32-His430; R&D Systems, 688-TK) and control human IgG-Fc (110-HG ...
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bioRxiv - Microbiology 2024Quote: 107 parasites were incubated with 0.5 μg recombinant human IgG Fc (R&D Systems, cat# 110-HG) or recombinant CD36-Fc (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... blocked with Mouse on Mouse Blocking Reagent (R&D Systems) 2h and incubated with primary antibody ...
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bioRxiv - Synthetic Biology 2022Quote: ... membranes were probed with both an anti–6x-His tag antibody (R&D Systems, Cat # MAB050; diluted 1:7,500) and anti–ETEC O148 antibody (Abcam ...
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bioRxiv - Immunology 2023Quote: Culture media of cells infected with virus were collected at the indicated times and used for ELISA quantification of IFNβ (R&D Systems) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were coated with 0.5 μg/mL rhCTLA-4-Fc chimera (7268-CT, R&D Systems, Minneapolis, MN, USA) diluted in 1× PBS pH 7.0 and incubated at 2-8°C overnight ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... The mLTα-Fc CM was quantitated by ELISA with goat polyclonal LTα capture antibody (R&D systems AF749), rat monoclonal LTα detection antibody (R&D systems MAB749 ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated for 1h at 37°C with 5nM of human SEMA3A Fc chimera (R&D System), dissolved in 1x Dulbecco’s PBS (DPBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Control cells were incubated with soluble hVEGFR1 (VEGFR1-Fc, 1 μg/mL; R&D Systems #321-FL-050). BODIPY FL C16 (1 μM ...
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bioRxiv - Developmental Biology 2024Quote: ... we pre-treated 1 mL of FBS with 2.5 mg of recombinant human ALK1-Fc (R&D systems) and rocked at room temperature for 2 hours before adding to 49 mL EGM2 for a final concentration of 50 µg/mL ALK1-Fc ...
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bioRxiv - Immunology 2021Quote: ... E-Selectin and Intercellular Adhesion Molecule (ICAM)-1 were both measured using ELISA assays (R&D Systems, Minneapolis, MN). Lot-to-lot changes in both E-Selectin and ICAM-1 assays were made throughout the course of the study and adjusted prior to the data being made publicly available to the public ...
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bioRxiv - Pathology 2021Quote: ... Slides from paraffin-embedded sections were H&E stained or immunostained with antibodies for: VEGFR3 (R&D Systems, AF743) or CCL21 (R&D Systems ...
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bioRxiv - Biophysics 2022Quote: ... the gels were washed twice with 1xPBS and incubated at room temperature with a drop of 25μL of 0.01mg·mL-1 histidine- tagged E-cadherin (8505-EC-050, R&D Systems) covered with rectangles of parafilm ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was also measured using 5 nM of recombinant human 26S proteasome (Cat: E-365; R&D Systems).
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... recombinant mouse C5a (500ng/mouse; R&D Systems, Minneapolis, MN, USA), and recombinant mouse CXCL4 (500ng/mouse ...
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bioRxiv - Biochemistry 2021Quote: ... TNFα and IL-6 in supernatants were measured using ELISAs (Mouse TNFα; Mouse CCL5; Mouse IL-6, R&D Systems) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2022Quote: ... wells of an 8-well microslide were coated with 10 μg/mL of ICAM-1/Fc (R&D systems) for 2 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... The binding of pig and rabbit (Thymoglobulin®, Sanofi) IgG to human FcγRIIIa (CD16a, R&D Systems, 4325-FC) receptor was also measured by SPR ...
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bioRxiv - Cell Biology 2021Quote: ... NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems, USA) and then FITC-conjugated anti-human Fc secondary antibody was used as a detection antibody (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... were diluted to 4 µg/ml in PBS along with 1 µg/ml VCAM-1-Fc (R&D Systems). To coat ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... and recombinant mouse CXCL4 (500ng/mouse; R&D Systems, Minneapolis, MN, USA). Sham treated mice received an equal volume of PBS i.t ...
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bioRxiv - Neuroscience 2020Quote: ... crassiceps excretory/secretory (E/S) product using commercially available ELISA reagents according to the manufacturer’s instructions (R&D Systems, Germany). 96 well plates were coated with 50 μl antibody solution (IL-1β ...
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bioRxiv - Biochemistry 2024Quote: ... carboxylesterase 1 (CES1) and trans-epoxy-succinyl-L-leucylamino(4-guanidino)butane (E-64) were purchased from R&D Systems. The respective genes coding for the human recombinant enzymes Histidine triad nucleotide binding protein 1 (HINT1) ...
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bioRxiv - Neuroscience 2024Quote: ... is sf21 (baculoviris)-derived mouse CX3CL1/Fractalkine protein of 34 kDa and 312 amino acids and recombinant soluble Fractalkine (R&D systems, cat. #571-MF, Accession# AAB71763) is E ...
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bioRxiv - Biophysics 2020Quote: ... Purified mouse CD45RABC extracellular domain with a C-terminal 6-His tag (accession #: NP_001104786, catalog number: 114-CD-050) was purchased from R&D systems. Lectins Sambucus Nigra Lectin (SNA ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were resuspended in an antibody solution containing 5μL DYKDDDDK Epitope Tag Alexa Fluor 647-conjugated Antibody (R&D Systems #IC8529R) and 45μL DBPS + 10% FBS and incubated in the dark at room temperature for one hour ...
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bioRxiv - Biochemistry 2022Quote: ... The cell layers were incubated for 1 hour with 10 µg per ml monoclonal anti-His6-tag antibody (R&D Systems) or a 1:1000 dilution of pAb 9543 279 anti-fibrillin-1 ...
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bioRxiv - Bioengineering 2023Quote: ... either 5μg or 2.5μg of the S protein or NC protein (R&D Systems, cat# 10474-CV-050 and cat# 10549-CV-100) ...
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bioRxiv - Biophysics 2019Quote: ... Ibidi channels IV0.4 (Clinisciences) were coated overnight at 4°C with 10μg/ml human ICAM-l-Fc (R&D Systems) in Phosphate Buffer Solution (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... During the first 10min, sections were superfused with either vehicle (i.e., 0.1%BSA/1xDPBS) or 100nM human SEMA3A Fc chimera (R&D System) diluted in warm (37°C ...
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bioRxiv - Immunology 2022Quote: ... The concentration of samples was extrapolated from standard curves of recombinant human IL7R-Fc chimera (R&D Systems, # 306-IR).
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bioRxiv - Immunology 2019Quote: ... cover glasses were coated for 2 h at 37°C with 100 nM recombinant ICAM1-FC (796IC, R&D Systems) and washed 2 times with PBS ...
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bioRxiv - Neuroscience 2021Quote: Human soluble E-selectin levels were assayed using quantitative sandwich enzyme immunoassay technique and following the manufacturer’s protocol (Quantikine ELISA Human E-selectin/CD62E Immunoassay, DSLE00, R&D Systems). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were fixed for 10 min in ice-cold methanol and incubated with primary antibodies (goat E-cadherin, R&D systems) and nuclear stain (Hoechst ...
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bioRxiv - Neuroscience 2020Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
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bioRxiv - Biochemistry 2021Quote: Human CD38 (P28907, a soluble fragment Val43-Ile300 with 6x-His tag at C terminus) was purchased from R&D Systems (USA). It was diluted to final 0.01 mg/ml in 100 mM HEPES/NaOH buffer ...
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bioRxiv - Biophysics 2022Quote: ... ECD fragments (modified C-terminally with 10xHis tag) Podocalyxin (PODXL) (Cat.# 1658-PD) and PODXL2 (Cat.# 1524-EG) were obtained from R&D Systems. The ECD fragments were dissolved in ultrapure water to a concentration of 0.5 mg/mL and aliquoted in 50 μL ...
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bioRxiv - Developmental Biology 2019Quote: ... A biotin conjugated anti-His-tag antibody (NB100-63172, Bio-Connect) was added followed by a Streptavidin-HRP (DY998, R&D Systems). For a colormetric detection ...
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bioRxiv - Biophysics 2024Quote: Recombinant sKLA (Glu34-Ser981) with a C-terminal 6His tag expressed in a NS0 murine myeloma cell line was purchased (R&D Systems) and loaded onto a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Physiology 2019Quote: ... Mouse GDF15 levels were measured using a Mouse GDF15 DuoSet ELISA (R&D Systems) which had been modified to run as an electrochemiluminescence assay on the Meso Scale Discovery assay platform.
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bioRxiv - Cancer Biology 2023Quote: ... Mouse peripheral cytokines were measured using the Mouse Proteome Profiler Kit (R&D Systems) following the manufacturer’s protocol on 100uL of mouse serum from whole blood.
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bioRxiv - Developmental Biology 2020Quote: ... Mouse EPO (R&D systems) was added to a final concentration of 10 units/ml ...
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bioRxiv - Cell Biology 2021Quote: ... NHEKs were induced to differentiate by addition of 1.2 mM CaCl2 to growth medium with or without addition of 1 μg/mL ephrin A1-FC peptide (R&D Systems) for the indicated times ...
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bioRxiv - Immunology 2022Quote: ... then stained in the presence of Fc block for 30 minutes at 4C using the following antibodies: CD4 (1:300; clone GK1.5; R&D Systems); CD44 (1:300 ...