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351 - 400 of 1831 citations for GOLM1 Human HEK 293 His since 2019

• Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors

  Chih-Wei Chu, et al., bioRxiv - Immunology 2023

  Quote: Serum and recombinant PD-1 was quantified by human PD-1 Quantikine ELISA and human PD-1 DuoSet ELISA (R&D Systems, Minneapolis, MN), following manufacturer’s protocol ...
• Maternal obesity driven changes in collagen linearity of breast extracellular matrix induces invasive mammary epithelial cell phenotype

  Jensen N. Amens, et al., bioRxiv - Bioengineering 2022

  Quote: ... was determined using the dot blot-based mouse XL cytokine array kit for the model group breast tissues and human XL cytokine array kit and human XL oncology array kit for the human cell conditioned media following manufacturer’s instructions (R&D Systems, Cat. No: ARY028). Briefly ...
• GSK-3 inhibitor elraglusib enhances tumor-infiltrating immune cell activation in tumor biopsies and synergizes with anti-PD-L1 in a murine model of colorectal cancer

  Kelsey E. Huntington, et al., bioRxiv - Cancer Biology 2023

  Quote: Human cell line culture supernatants were analyzed using an R&D systems Human Premixed Multi-Analyte Kit (R&D Systems, Inc., Minneapolis, MN, USA) and a Luminex 200 (RRID ...
• Allosteric modulation of the CXCR4:CXCL12 axis by targeting receptor nanoclustering via the TMV-TMVI domain

  Eva M. García-Cuesta, et al., bioRxiv - Cell Biology 2023

  Quote: The following antibodies were used: monoclonal mouse anti-human CXCR4 (clone 44717) and phycoerythrin-conjugated human CXCR4 (clone 12G5; both from R&D Systems, Minneapolis, MN); purified mouse anti-human CD3 (clone HIT3a ...
• PARP inhibitors enhance reovirus-mediated cell killing through the death-inducing signaling complex (DISC) with an associated NF-κB-regulated immune response

  Joan Kyula-Currie, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cytokine secretion was carried out as a validation from the results of the human cytokine array using a range of human ELISAS from R&D Systems (see below).
• Eicosatetraynoic Acid Regulates Pro-Fibrotic Pathways in an Induced Pluripotent Stem Cell Derived Macrophage:Human Intestinal Organoid Model of Crohn’s Disease

  Ingrid Jurickova, et al., bioRxiv - Molecular Biology 2024

  Quote: ... CCL2 and TL1A were measured using human CCL2 (MCP-1) and human TL1A ELISA kits by R&D Systems (R&D Systems, Minneapolis, MN). SEMA3C was measured using the human SEMA3C ELISA kit by LS Bio (LS Bio ...
• Desert Hedgehog-driven endothelium integrity is enhanced by Gas1 but negatively regulated by Cdon

  Candice Chapouly, et al., bioRxiv - Cell Biology 2020

  Quote: ... goat anti-human Gas1 antibodies (R&D systems, Cat# AF2636), mouse anti-Dhh antibodies (Santa Cruz Biotechnology ...
• Zn²⁺ influx activates ERK and Akt signaling pathways through a common mechanism

  Kelsie J. Anson, Giulia A. Corbet, Amy E. Palmer, bioRxiv - Cell Biology 2020

  Quote: Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems) was used to measure changes in phosphorylation of 43 kinases upon 40 μM Zn2+ treatment ...
• Single Nucleotide Polymorphisms in the Bovine TLR2 Extracellular Domain Contribute to Breed and Species-Specific Innate Immune Functionality

  Marie-Christine Bartens, et al., bioRxiv - Immunology 2021

  Quote: ... or Quantikine Human CXCL8 ELISA kit (R&D Systems, USA) for human MØ ...
• Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity

  Nihal Karakaş, et al., bioRxiv - Neuroscience 2020

  Quote: ... recombinant human brain-derived neurotrophic factor (BDNF; R&D systems), and nerve growth factor (NGF) ...
• Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity

  Nihal Karakaş, et al., bioRxiv - Neuroscience 2020

  Quote: ... recombinant human basic fibroblast growth factor (bFGF; R&D systems), fibroblast growth factor-8 (FGF-8 ...
• Nephronectin-Integrin α8 signaling is required for proper migration of periocular neural crest cells during chick corneal development

  Justin Ma, Lian Bi, James Spurlin, Peter Lwigale, bioRxiv - Developmental Biology 2021

  Quote: ... followed by recombinant mouse or human Npnt (R&D Systems) at 1.5 μg/cm2 for 2 hours at 37 °C ...
• The Structure-Selective Endonucleases GEN1 and MUS81 are Functionally Complementary in Safeguarding the Genome of Proliferating B Lymphocytes

  Keith Conrad Fernandez, et al., bioRxiv - Immunology 2022

  Quote: ... 2 ng/mL recombinant human TGF-β1 (R&D Systems), and 333 ng/mL anti-IgD dextran conjugates (Fina Biosolutions) ...
• Crosstalk between Fat Mass and Obesity-related (FTO) and multiple WNT signaling pathways

  Hyunjoon Kim, Soohyun Jang, Young-suk Lee, bioRxiv - Molecular Biology 2021

  Quote: ... and human R-Spondin1 (R&D Systems #4645-RS-025) were purchased and used ...
• The Role of Notch Signaling in Endometrial Mesenchymal Stromal/Stem-like Cells Maintenance

  Sisi Zhang, et al., bioRxiv - Cell Biology 2022

  Quote: ... or recombinant human WNT5A (0.01 μg/ml, R&D Systems) was supplemented to eMSC cultured in GM ...
• Nucleation of the destruction complex on the centrosome accelerates degradation of β-catenin and regulates Wnt signal transmission

  Ryan S. Lach, et al., bioRxiv - Cell Biology 2022

  Quote: Recombinant Human Wnt-3a (R&D Systems 5036-WN-010) was resuspended in in PBS containing 0.1% BSA according to supplied manufacturer recommendations and diluted to 5X concentration in culture medium immediately prior to use ...
• A dual mechanism of enhancer activation by FOXA pioneer factors induces endodermal organ fates

  Ryan J. Geusz, et al., bioRxiv - Genomics 2020

  Quote: ... 10 ng/mL recombinant human BMP4 (rhBMP4, R&D Systems), and 100 nM retinoic acid (RA ...
• Conserved α-helix-3 is crucial for structure and functions of Rad6 E2 ubiquitin-conjugating enzymes

  Prakash K. Shukla, et al., bioRxiv - Molecular Biology 2021

  Quote: ... 2.5 µM recombinant yeast or human ubiquitin (R&D Systems), 0.1 µM wild-type or mutant Rad6 or UBE2B (E2) ...
• Congruence of location-specific transcriptional programs in intestinal organoids during long-term culture

  Bart van der Hee, et al., bioRxiv - Cell Biology 2019

  Quote: ... 50 ng/ml human epidermal growth factor (R&D systems), 15 nM gastrin ...
• Systemic inflammation and risk of age-associated diseases in people living with HIV on long term suppressive antiretroviral therapy

  Hemalatha Babu, et al., bioRxiv - Immunology 2019

  Quote: ... sCD14 (Human CD14 Quantikine ELISA Kit R&D Systems, UK) and sCD163 (Thermo Scientific™ Pierce™ Human CD163 Kit ...
• IκBζ is constitutively expressed in human and murine airway epithelium

  Kruthika Sundaram, et al., bioRxiv - Immunology 2019

  Quote: ... recombinant human IL-1β was purchased from R&D systems. Rabbit antiserum against IκBζ was generated in our laboratory using recombinant protein expressed in Escherichia coli (8 ...
• Fibroblastic reticular cells provide a supportive niche for lymph node-resident macrophages

  Joshua D’Rozario, et al., bioRxiv - Immunology 2021

  Quote: ... or anti-human isotype control (Clone: 11711 – R&D systems) were added ...
• Fibroblastic reticular cells provide a supportive niche for lymph node-resident macrophages

  Joshua D’Rozario, et al., bioRxiv - Immunology 2021

  Quote: ... 0.1μg/ml human anti-CSF1R (Clone: 61701 – R&D systems) or anti-human isotype control (Clone ...
• CARPOOL: A library-based platform to rapidly identify next generation chimeric antigen receptors

  Taeyoon Kyung, et al., bioRxiv - Immunology 2021

  Quote: ... 100 IU/ml recombinant human IL-2 (R&D Systems), and 50 μM beta-mercaptoethanol (Fisher) ...
• Interfacing Live Cells with Surfaces: A Concurrent Control Technique for Quantifying Surface Ligand Activity

  Michael C. Robitaille, et al., bioRxiv - Cell Biology 2021

  Quote: Recombinant human αVβ3 integrins (R&D Systems, #3050-AV-050) were reconstituted in 20 mM Tris buffered saline (TBS) ...
• Identification and Characterization of Stem Cells in Mammalian Esophageal Stratified Squamous Epithelia

  Yanan Yang, et al., bioRxiv - Cell Biology 2021

  Quote: ... 100 ng/mL recombinant human R-Spondin1 (R&D Systems) and 10 μM Y27632 (Topscience ...
• CITEseq analysis of non-small-cell lung cancer lesions reveals an axis of immune cell activation associated with tumor antigen load and TP53 mutations

  Andrew M. Leader, et al., bioRxiv - Immunology 2020

  Quote: ... Primary antibodies were: anti-human CD10 (200103, R&D systems), DC-Lamp (1010E1.01 ...
• BSG/CD147 and ACE2 receptors facilitate SARS-CoV-2 infection of human iPS cell-derived kidney podocytes

  Titilola D. Kalejaiye, et al., bioRxiv - Bioengineering 2021

  Quote: ... Human/Mouse/Rat/Hamster ACE-2 (R&D systems, AF933), Human TRA-1-85/CD147 (R&D systems ...
• Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosis

  Alex Rosenberg, L. David Sibley, bioRxiv - Microbiology 2020

  Quote: ... Bradyzoites were stimulated with human IFN-γ (R&D systems) at final experimental concentration of 100 U/ml for 6 hr after 5 days in differentiation media prior to being washed with PBS and dislodged with trypsin ...
• Dynamic optimization reveals alveolar epithelial cells as key mediators of host defense in invasive aspergillosis

  Jan Ewald, et al., bioRxiv - Systems Biology 2021

  Quote: ... 50ng/mL human fibroblastic growth factor 10 (R&D system), 5% FBS ...
• SARS-CoV-2 Infection of Pluripotent Stem Cell-derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response

  Jessie Huang, et al., bioRxiv - Molecular Biology 2020

  Quote: ... 10 ng/mL recombinant human BMP4 (rhBMP4, R&D Systems), and 100nM retinoic acid (RA ...
• Overturning the paradigm that IL6 signaling drives liver regrowth while shining light on a new therapeutic target for regenerative medicine

  Jinrui Dong, et al., bioRxiv - Cell Biology 2021

  Quote: ... human soluble gp130 Fc (671-GP-100, R&D systems).
• Nuclear stiffness decreases with disruption of the extracellular matrix in living tissues

  Kaitlin P. McCreery, et al., bioRxiv - Biophysics 2020

  Quote: Recombinant human ADAMTS-4 (R&D Systems, Minneapolis, MN, USA) and MMP-13 (R&D Systems ...
• FTY720 requires vitamin B₁₂-TCN2-CD320 signaling in astrocytes to reduce disease in an animal model of multiple sclerosis

  Deepa Jonnalagadda, et al., bioRxiv - Neuroscience 2022

  Quote: ... Recombinant human CD320 (R&D systems, cat # 1557-CD-050) and human TCN2 (R&D systems ...
• 3D-imaging reveals conserved cerebrospinal fluid drainage via meningeal lymphatic vasculature in mice and humans

  Laurent Jacob, et al., bioRxiv - Neuroscience 2022

  Quote: ... Goat anti– human PROX1 (AF2727; R&D Systems;1:1000), Rabbit anti-mouse TUJ1 (802001 ...
• IL11 stimulates ERK/P90RSK to inhibit LKB1/AMPK and activate mTOR in hepatocytes, the stroma and cancer cells

  Anissa A. Widjaja, et al., bioRxiv - Molecular Biology 2022

  Quote: Commercial recombinant proteins: human IL6 (206-IL, R&D Systems), human insulin (I9278 ...
• FTY720 requires vitamin B₁₂-TCN2-CD320 signaling in astrocytes to reduce disease in an animal model of multiple sclerosis

  Deepa Jonnalagadda, et al., bioRxiv - Neuroscience 2022

  Quote: ... and human TCN2 (R&D systems, cat # 7895-TC-050) were mixed with FTY720 (Novartis) ...
• PVT1, a YAP1 dependent stress responsive lncRNA drives ovarian cancer metastasis and chemoresistance

  Kevin Tabury, et al., bioRxiv - Cancer Biology 2022

  Quote: ... fibronectin (Recombinant Human Fibronectin R&D Systems, 8258-FN-050); bovine serum albumin (BSA ...
• Anti-inflammatory role of GM1 and modulatory effects of gangliosides on microglia functions

  Danny Galleguillos, et al., bioRxiv - Neuroscience 2020

  Quote: ... and Human IL-1β DuoSet ELISA (DY201, R&D Systems). BDNF and IGF-1 in cell medium and cell lysates of mouse primary microglia were measured using Mature BDNF Rapid ELISA kit (Biosensis ...
• Physiological activation of the nephron central command drives endogenous kidney tissue regeneration

  Georgina Gyarmati, et al., bioRxiv - Physiology 2021

  Quote: Human CCN1 ELISA assay (R&D systems, Minneapolis, MN, USA) was used to analyze urinary CCN1 in undiluted samples collected from control (n=11 ...
• The effect of paracrine factors released by irradiated peripheral blood mononuclear cells on neutrophil extracellular trap formation

  Katharina Klas, et al., bioRxiv - Immunology 2022

  Quote: Human Apoptosis Array kit (R&D Systems, Minneapolis, MN, USA) was performed in accordance to manufacturer’s instructions with no modifications ...
• Single-cell transcriptomics reveal hyperacute cytokine and immune checkpoint axis in patients with poor neurological outcomes after cardiac arrest

  Tomoyoshi Tamura, et al., bioRxiv - Immunology 2022

  Quote: ... All recombinant human cytokines were purchased from R&D Systems. After 6h of incubation ...
• RSV protects bystander cells against IAV infection by triggering secretion of type I and type III interferons

  Maciej Czerkies, et al., bioRxiv - Immunology 2022

  Quote: ... anti-human interferon beta antibody (R&D Systems, cat. MAB8142).
• Reconstitution of human adrenocortical specification and steroidogenesis using induced pluripotent stem cells

  Yuka Sakata, et al., bioRxiv - Developmental Biology 2022

  Quote: ... 50 ng/mL human sonic hedgehog/SHH (R&D Systems) and 10 μM Y-27632 ...
• Oleocanthal and oleocanthal-rich olive oils induce lysosomal membrane permeabilization in cancer cells

  Limor Goren, et al., bioRxiv - Cancer Biology 2019

  Quote: ... goat anti-human Cathepsin B antibody (R&D systems AF953), goat anti human cathepsin-D antibody (Santa Cruz sc-6486) ...
• Human caspase-1 autoproteolysis is required for ASC-dependent and -independent inflammasome activation

  Daniel P. Ball, et al., bioRxiv - Immunology 2019

  Quote: ... human NLRP1/NALP1 Sheep polyclonal antibody (R&D systems, AF6788), V5 Rabbit polyclonal Ab (Abcam ...
• De novo mutations in fetal brain specific enhancers play a significant role in severe intellectual disability

  Matias G De Vas, et al., bioRxiv - Genomics 2019

  Quote: ... and 2 ng/mL recombinant human GDNF (R&D Systems).
• Myosin II isoforms play distinct roles in adherens junction biogenesis

  Mélina L. Heuzé, et al., bioRxiv - Cell Biology 2019

  Quote: ... and 1µg of recombinant human E-cadherin (R&D systems). Coverslips were then washed two times with PBS.
• Piezo1 channel activates ADAM10 sheddase to regulate Notch1 and gene expression

  Vincenza Caolo, et al., bioRxiv - Cell Biology 2019

  Quote: ... goat anti human VEGFR2 (AF357, R&D system, Minneapolis, MN), mouse anti-human PECAM-1 (CD31 ...
• Enhancing Cardiac Reprogramming by Suppressing Specific C-C Chemokine Signaling Pathways

  Yijing Guo, et al., bioRxiv - Cell Biology 2019

  Quote: ... and recombinant human FGF10 (25 ng/ml, R&D Systems). Then mature medium was changed every 2–3 days for another 2 weeks as previously described(17).