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1 - 50 of 3978 citations for Elongator complex protein 1 ELP1 Antibody Biotin since 2020

• Exploiting the Cullin E3 Ligase Adaptor Protein SKP1 for Targeted Protein Degradation

  Seong Ho Hong, et al., bioRxiv - Biochemistry 2023

  Quote: ... The SKP1 containing SCF complex was reconstituted by utilizing stoichiometric amount of the following recombinant proteins: Recombinant Human CUL1/RBX1 Neddylated Complex Protein (R&D systems, E3-411-025), Recombinant Human FBXO7 GST (N-Term ...
• Targeting triple-negative breast cancer with β1-integrin binding aptamer

  Karlis Pleiko, et al., bioRxiv - Molecular Biology 2022

  Quote: ... α3β1-integrin protein complex (#2840-A3-050, R&D Systems, 20 µL) at 360 nM (100 µg/mL ...
• Sequentially activated death complexes regulate pyroptosis and IL-1β release in response to Yersinia blockade of immune signaling

  Ronit Schwartz Wertman, et al., bioRxiv - Immunology 2023

  Quote: ... plates were incubated with biotin-linked anti-IL-1β detection antibody (R&D Systems, 1:1000), followed by horseradish peroxidase-conjugated streptavidin ...
• Deconvolution Of Hematopoietic Stem/Progenitor Cell Signaling Predicts Inflammatory Niche Remodeling To Be A Determinant Of Tissue Failure And Outcome In Human AML

  Lanpeng Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and LEPR-biotin (1:50, catalog RB01, R&D systems) followed by staining of streptavidin-APC (1:100 ...
• Conversion of natural cytokine receptors into orthogonal synthetic biosensors

  Hailey I. Edelstein, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... 2.5 µL anti-hEGF tag antibody (Anti-hEGF-biotin, R&D Systems BAF236) was added at a concentration of 0.5 µg per sample and cells incubated at 4 °C for 20 min ...
• Targeting PD-L1 in solid cancer with myeloid cells expressing a CAR-like immune receptor

  Kayla Myers Chen, et al., bioRxiv - Immunology 2024

  Quote: ... following reagents were used in flow cytometry: recombinant human PD-L1 Fc chimera Biotin protein (R&D Systems); APC Streptavidin (Tonbo Biosciences) ...
• Multi-tier mechanics control stromal adaptations in swelling lymph nodes

  Frank P. Assen, et al., bioRxiv - Immunology 2021

  Quote: ... α-CCL21-Biotin (R&D Systems), α-PDPN-Biotin (8.1.1 ...
• Genetically-Modified Macrophages Accelerate Myelin Repair

  Marie-Stephane Aigrot, et al., bioRxiv - Neuroscience 2022

  Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
• Tissue damage during acute Trypanosoma cruzi infection is associated with reduced reparative regulatory T cell response and can be attenuated by early interleukin-33 administration

  Santiago Boccardo, et al., bioRxiv - Immunology 2024

  Quote: Combinations of the following antibodies were used for flow cytometry: biotin polyclonal anti-Amphiregulin (R&D Systems), PE anti-BATF clone S39-1060 (BD Pharmingen) ...
• Chronobiological rhythms control of site- and cell-specific miRNA and mRNA genes and networks across the central nervous system

  Amanda M. Zacharias, et al., bioRxiv - Neuroscience 2024

  Quote: ... Primary antibodies for proteins of interest were anti-IL-33 (1:50, cat.no.AF3626, R&D Systems), anti-AdipoR2 (1:200 ...
• Sustained IL-15 response signature predicts RhCMV/SIV vaccine efficacy

  Fredrik Barrenäs, et al., bioRxiv - Genomics 2021

  Quote: ... CCR7 (15053; Biotin, R&D Systems), streptavidin (Pacific Blue ...
• Expansions of adaptive-like NK cells with a tissue-resident phenotype in human lung and blood

  Nicole Marquardt, et al., bioRxiv - Immunology 2020

  Quote: ... KIR2DL1 (FAB1844F, biotin, R&D Systems), KIR2DL3 (180701 ...
• Tissue-resident regulatory T cells exert dualistic anti-tumour and pro-repair function in the exocrine pancreas

  Julie Stockis, et al., bioRxiv - Immunology 2024

  Quote: ... Areg (polyclonal, R&D Systems, biotin), CD140a (APA5 ...
• Substrate stiffness engineered to replicate disease conditions influence senescence and fibrotic responses in primary lung fibroblasts

  Kaj E.C. Blokland, et al., bioRxiv - Cell Biology 2022

  Quote: ... Total RANKL was calculated by measuring free RANKL and in complex with OPG using an in house developed OPG/RANKL complex sandwich ELISA by using a human capture RANKL antibody (part of cat#DY626, R&D Systems) and a human detection OPG antibody (part of cat#DY805 ...
• The helminth glycoprotein omega-1 improves metabolic homeostasis in obese mice through type-2 immunity-independent inhibition of food intake

  Hendrik J.P. van der Zande, et al., bioRxiv - Immunology 2020

  Quote: ... Cells were then incubated with an antibody against YM1 conjugated to biotin (polyclonal; R&D Systems, Minneapolis, MN, USA), washed ...
• Rational screening for cooperativity in small-molecule inducers of protein–protein associations

  Shuang Liu, et al., bioRxiv - Biophysics 2023

  Quote: ... Tagless VCB complex was purchased from R&D Systems (E3-600) excluding DTT in the supplied buffer to ensure compatibility with the His-Tag Dynabeads ...
• SOX9 acts as a dynamic pioneer factor inducing stable changes in the chromatin landscape to reprogram endothelial cells

  Bettina M. Fuglerud, et al., bioRxiv - Genomics 2021

  Quote: ... Primary antibodies against the following proteins were used: SOX9 (1:50, AF3045, R&D Systems, and AB5535, Millipore), ERG (1:50 ...
• S100A8 and S100A9, biomarkers of SARS-Cov2-infected patients, suppress HIV replication in primary macrophages

  Raphael M. Oguariri, et al., bioRxiv - Microbiology 2021

  Quote: ... (Beijing, China) and S100A8/S100A9 complex was purchased from R&D systems. Anti-SPTBN and anti-b-actin antibodies were obtained from Abcam (Waltham ...
• Oxidant-Resistant LRRC8A/C Anion Channels Support Superoxide Production by Nox1

  Hyehun Choi, et al., bioRxiv - Cell Biology 2021

  Quote: ... Human TNFα conjugated with biotin (R&D Systems) was incubated with FITC-labeled avidin (Life Technologies ...
• Expansions of adaptive-like NK cells with a tissue-resident phenotype in human lung and blood

  Nicole Marquardt, et al., bioRxiv - Immunology 2020

  Quote: ... NKG2C (134591, biotin, R&D Systems, custom conjugate), CD56 (NCAM16.1 ...
• Ubr1-induced selective endo-phagy/autophagy protects against the endosomal and Ca²⁺-induced proteostasis disease stress

  Ben B. Wang, et al., bioRxiv - Biochemistry 2021

  Quote: ... Selective isolation of MLC1-complex from the PM was achieved by cs-IP using anti-GlialCAM Ab (1:2000, R&D Systems). Ab was bound on ice to live cells for 45min ...
• CellStudio: a Modular, Tunable and Accessible Platform for Analysis of Growth Factors Secretions in Cell Cultures

  Enrique Azuaje-Hualde, et al., bioRxiv - Cell Biology 2024

  Quote: ... and 5 µg mL-1 biotin Polyclonal Goat anti-Fibroblast Growth Factor IgG solution (R&D Systems, USA), for the generation of anti-FGF-2 functionalized beads ...
• CellStudio: a Modular, Tunable and Accessible Platform for Analysis of Growth Factors Secretions in Cell Cultures

  Enrique Azuaje-Hualde, et al., bioRxiv - Cell Biology 2024

  Quote: ... 5 µg mL-1 biotin Polyclonal Goat anti-Vascular Endothelial Growth Factor IgG solution (R&D Systems, USA), for the generation of anti-VEGF functionalized beads ...
• Prostaglandin E₂ induction by cytosolic Listeria monocytogenes in phagocytes is necessary for optimal T-cell priming

  Courtney E. McDougal, et al., bioRxiv - Immunology 2021

  Quote: ... anti-MARCO polyclonal Goat IgG-Biotin (R&D Systems, BAF2956), and FITC-conjugated COX2 polyclonal antibody (Cayman Chemical ...
• Targeting caveolae to pump bispecific antibody to TGF-β into diseased lungs enables ultra-low dose therapeutic efficacy

  Anil H. Kadam, et al., bioRxiv - Bioengineering 2022

  Quote: ... anti-TGF-β1 IgY-Biotin (R&D Systems, Minneapolis, MN), anti-hIgG-HRP (Jackson ImmunoResearch Inc. ...
• Cell surface ribonucleoproteins cluster with heparan sulfate to regulate growth factor signaling

  Peiyuan Chai, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Siglec-1 Fc chimera protein (R&D Systems), Siglec-2 Fc chimera protein (R&D Systems) ...
• Epigenetic therapy remodels the immune synaptic cytoskeleton to potentiate cancer susceptibility to γδ T cells

  Rueyhung R. Weng, et al., bioRxiv - Cancer Biology 2020

  Quote: ... The overexpression and loss of ICAM-1 protein were validated by flow cytometry with an anti-ICAM1 antibody (BBA20, R&D Systems). Besides ...
• The transcription factor Hhex cooperates with the corepressor Tle3 to promote memory B cell development

  Brian J. Laidlaw, et al., bioRxiv - Immunology 2020

  Quote: ... Biotin goat anti-mouse polyclonal Ephrin-B1 (BAF473) (R&D Systems); FITC anti-BrDU (11-5071-42 ...
• Stromal inflammation is a targetable driver of hematopoietic aging

  Evgenia V. Verovskaya, et al., bioRxiv - Cell Biology 2021

  Quote: ... sections were first stained with LepR-biotin (R&D systems, AF497) and rabbit anti-mouse laminin ...
• Whole genome CRISPR screens identify LRRK2-regulated endocytosis as a major mechanism for extracellular tau uptake by human neurons

  Lewis D. Evans, et al., bioRxiv - Neuroscience 2020

  Quote: ... Recombinant proteins were separately preincubated with neuronal cultures and recombinant tau protein for 3 hours prior to incubation of neuronal cultures with the mixer containing recombinant proteins (100nM Human LRP-1 Cluster IV Fc Chimera Protein; 10nM Human LRPAP Protein [R&D systems]). For pharmacological treatments ...
• Structural insights into CXCR4 modulation and oligomerization

  Kei Saotome, et al., bioRxiv - Biochemistry 2024

  Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
• A disrupted compartment boundary underlies abnormal cardiac patterning and congenital heart defects

  Irfan S. Kathiriya, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Anti-Netrin-1 antibody 1:500 (R&D Systems AF1109) in 1% BSA + 0.1% PBST was incubated at 4C overnight ...
• Platelet-derived transcription factors license human monocyte inflammation

  Ibrahim Hawwari, et al., bioRxiv - Immunology 2023

  Quote: ... Antibodies were anti-IL-1b antibody (BAF401, R&D Systems, 1:1,000). DRAQ5 was from Thermo Fisher Scientific ...
• Serum Amyloid A proteins reduce bone mass during mycobacterial infections

  Ana Cordeiro Gomes, et al., bioRxiv - Immunology 2022

  Quote: ... LEPR staining was performed using biotin-conjugated anti-LEPR (R&D systems) for 1 hour ...
• Quantitative modeling of regular retinal microglia distribution

  Yoshie Endo, et al., bioRxiv - Cell Biology 2021

  Quote: ... Recombinant Mouse sFRP-1 Protein (1 µg/ml, R&D Systems, MN9019-SF-025), Plexin-D1 Fc fragment (30 µg/ml) ...
• Formation of multinucleated globoid cells in Krabbe iPSC-derived microglia cultures: a preliminary investigation

  Richard Lieberman, et al., bioRxiv - Neuroscience 2021

  Quote: ... 1 nM or 10 nM rhGALC protein (R&D Systems) was added to the microglia medium at the time of seeding into the assay plate.
• Connexin 43 and Cell Culture Substrate Differentially Regulate OCY454 Osteocytic Differentiation and Signaling to Primary Bone Cells

  Gabriel A. Hoppock, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and dickkopf-related protein 1 (R&D Systems; Catalog #DY1756). All ELISA assays were run in duplicate according to manufacturer protocols ...
• Phagocytosis via complement receptor 3 enables microbes to evade killing by neutrophils

  Asya Smirnov, et al., bioRxiv - Microbiology 2022

  Quote: ... caninum NIF protein (R&D Systems; 0.5 µg ml−1) for 20 min ...
• More than a ligand: PD-L1 promotes oncolytic virus infection via a metabolic shift that inhibits the type I interferon pathway

  Jonathan J. Hodgins, et al., bioRxiv - Cancer Biology 2022

  Quote: ... recombinant mouse PD-1-Fc chimeric protein (R&D Systems) or human IgG1 control (R&D Systems) ...
• Diet-induced obesity results in endothelial cell desensitization to VEGF-A and permanent islet vascular dysfunction

  Yan Xiong, et al., bioRxiv - Physiology 2023

  Quote: ... anti-PECAM-1 antibody (goat, 1:400, R&D Systems, USA), anti-VEGFR2 (rabbit ...
• Integrative Clinical and Molecular Characterization of Translocation Renal Cell Carcinoma

  Ziad Bakouny, et al., bioRxiv - Cancer Biology 2021

  Quote: ... the anti-TIM3 antibody (1:1,000, AF2365 goat monoclonal antibody, R&D Systems) was detected using the Opal 540 fluorophore (1:50 ...
• Improved Heat Tolerance in Heat-acclimated Mice: The Probable Role of the PD-L1 Pathway

  Minyue Qiu, et al., bioRxiv - Immunology 2022

  Quote: ... All monoclonal antibodies and recombinant mouse CD274 protein were purchased from R&D Systems (USA).
• A stochastic pooling motif mediates information transfer from receptor assemblies into NF-κB

  J. Agustin Cruz, et al., bioRxiv - Systems Biology 2021

  Quote: ... and PE-conjugated antibodies against the following human proteins were obtained from R&D systems: TNFR1 (FAB225P) ...
• Optimization of long-term human iPSC-derived spinal motor neuron culture using a dendritic polyglycerol amine-based substrate

  Louise Thiry, et al., bioRxiv - Neuroscience 2021

  Quote: ... goat anti-FORKHEAD BOX PROTEIN 1 (FOXP1) (1/100; R&D Systems; Cat. No. AF4534); and mouse anti-neurofilament protein (2H3 ...
• Characterization of human-iPSCs derived spinal motor neurons by single-cell RNA sequencing

  Louise Thiry, et al., bioRxiv - Neuroscience 2020

  Quote: ... goat anti-FORKHEAD BOX PROTEIN 1 (FOXP1) (1/100; R&D Systems; Cat. No. AF4534), and guinea pig anti-SCIP (1/16,000 ...
• Generation of human iPSC-derived phrenic-like motor neurons to model respiratory motor neuron degeneration in ALS

  Louise Thiry, et al., bioRxiv - Neuroscience 2024

  Quote: ... goat anti-FORKHEAD BOX PROTEIN 1 (FOXP1) (1/100; R&D Systems; Cat. No. AF4534); mouse anti-neurofilament protein (2H3 ...
• Jagged1 is a Notch-independent mechanotransducer in endothelial cells

  Freddy Suarez Rodriguez, et al., bioRxiv - Cell Biology 2024

  Quote: ... or recombinant peptides (Recombinant Human Notch-1 Fc chimera protein and IgG-Fc chimera protein from R&D systems). Cells were incubated with beads at 37°C ...
• Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae, to the liver

  Hitoshi Tsugawa, et al., bioRxiv - Microbiology 2023

  Quote: ... or 1 μg of human recombinant Gas6 protein (R&D Systems) prior to K ...
• Non-cell-autonomous control of gastruloid development by the lncRNA T-UCstem1 through DKK1-dependent modulation of WNT signalling

  Arianna Coppola, et al., bioRxiv - Developmental Biology 2024

  Quote: ... DKK-1 recombinant protein (R&D systems, cat: 5439-10 μg) was dissolved in PBS 1X containing at least 0.1% BSA at a concentration of 100 μg/mL and used at a concentration of 200 ng/mL ...
• The pericardium forms as a distinct structure during heart formation

  Hannah R. Moran, et al., bioRxiv - Developmental Biology 2024

  Quote: ... The sFRP1 (Recombinant Human sFRP-1 Protein, CF, R&D systems) solution was freshly prepared for each treatment by dissolving in phosphate-buffered saline (PBS ...