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Citations for R&D Systems :
551 - 600 of 947 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... ~70,000 cells/well were seeded in a 8-chamber slide coated with either 10μg/mL rNPNT (R&D system), 2μg/mL BSA ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed and incubated with an HRP conjugated rabbit anti-mouse antibody (1:5000 diluted, R&D systems) for 30 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the cell culture supernatants were collected and IL-17A and IFN-γ levels measured by ELISA (R&D systems) according to manufacturer specifications.
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bioRxiv - Immunology 2021Quote: ... and TNF-a in cell culture medium and serum was measured using commercially available ELISA kits (R&D Systems). SARS-CoV-2 S protein in cell lysates was detected by SARS-CoV-2 S ELISA kit and following manufacturer’s instruction (RayBiotech ...
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bioRxiv - Immunology 2020Quote: CH12 cells were cultured for 72h in the presence of TGF-β (1 ng/ml; R&D Systems Europe), IL-4 (5 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... were isolated by negative selection with the Mouse Memory T cell CD4+/CD62L−/CD44hi Column Kit (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... Mouse IL-6 was detected from cell culture supernatants by ELISA according to the manufacturer’s instructions (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... IL-4 in cell culture supernatants or peritoneal washes were determined using DuoSet ELISA Development kits (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems, USA) and then FITC-conjugated anti-human Fc secondary antibody was used as a detection antibody (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 in cell supernatants was determined by ELISA following the manufacturer’s instructions (R&D Systems). Results were read in a Synergy Mx plate reader (BioTek).
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bioRxiv - Neuroscience 2020Quote: ... Cells were treated with the indicated neuropilin 1 ligands Semaphorin 3A (3nM; Cat#5926-S3-025, R&D Systems), VEGF-A165 (1nM ...
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bioRxiv - Immunology 2022Quote: ... Cell supernatants were then harvested and assayed for cytokines by Human Cytokine Array (Proteome Profiler, R&D systems, ARY005B), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... BMDCs were generated from bone marrow cells treated with 10 ng/ml GM-CSF (R&D Systems, Minneapolis, MN) for 10 days ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ng/mL recombinant human glial cell line-derived neurotrophic factor (GDNF, #212-GD-010, R&D Systems, Germany), 500 µM cAMP (#D0260 ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested cells were washed three times with PBS and resuspended in 2/3 Cultrex Type 2 (R&D Systems) and 1/3 Full Ad-DF+++ medium and plated in drops which were allowed to solidify for 30 minutes at 37°C after which they were overlayed with Full Ad-DF+++ medium ...
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bioRxiv - Molecular Biology 2022Quote: ... CH12F3-2 cells were incubated in culture medium supplemented with10ng/mL IL-4 (R&D Systems #404-ML-050), 1ng/mL TGFβ (R&D Systems #7666-MB-005 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with antibodies that reacted with the extracellular domain of MT1-MMP (R&D Systems, Minnesota, USA) in the complete medium for 30 min on ice before fixation and permeabilisation ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were pelleted and then resuspended in 1 mL of Flow Cytometry Staining Buffer 1X (R&D Systems #FC001) for blocking ...
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bioRxiv - Genetics 2023Quote: ... This step was then repeated before embedding the cells in basement membrane extract (BME-R1) (R&D Systems, 3433010R1). Droplets measuring 15 μl were placed in a 48-well plate (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Purified cells from five subsets were cultured in IL-12 (20 ng/ml, 219-IL-005; R&D Systems), IL-18 (20 ng/ml 9124-IL-010 ...
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bioRxiv - Physiology 2023Quote: ... was quantified in cell culture media using a SDC4 ELISA as per the manufacturer’s instructions (DY2918, R&D Systems).
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bioRxiv - Cell Biology 2023Quote: ... plated cells were transferred into complete basal media supplemented with 0.05 ng/μL BMP4 (R&D systems, 5020-BP), 20 ng/mL FGF and 1 mg/mL laminin (Martynoga et al. ...
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bioRxiv - Immunology 2024Quote: ... Adaptive NK cell-positive donors were identified by anal-ysis of NKG2C (FAB138P or FAB138A antibodies, R&D Systems) and FcεRIγ (FCABS400F antibody ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then treated with compounds for 2 hours prior to TGF-β1 stimulation (1ng/ml, R&D systems. After 48 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were treated for 2 days (days 6, 7) with 50 ng/ml sonic hedgehog (SHH; R&D Systems), 2 μM dorsomorphin (DSM ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... at 3500 cells per well in GK15 medium supplemented with 200 ng/ml BMP4 (R&D Systems, 314-BP-010), 100 ng/ml SCF (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with PE-conjugated anti-CD140b antibody (2.5 μg/ml, PR7212 clone, Mouse IgG1, R&D Systems) and FITC-conjugated anti-CD146 antibodies (5 μg/ml ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated with a primary antibody of anti-Human/Mouse/Rat Vinculin mouse monoclonal (R&D systems; MAB68961-SP) diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam) ...
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bioRxiv - Systems Biology 2021Quote: ... cells were treated with ligand in fresh EM media as follows: 10 ng/ml EGF (R&D Systems #236-EG), 40 ng/ml HGF (R&D Systems #294-HG) ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were kept in 1.5ml Eppendorf tube and stimulated by adding 50nM CCL19 (R&D System 440-M3) and 50nM CCL21 (R&D System 457-6C ...
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bioRxiv - Cell Biology 2020Quote: ... Afterwards cells were incubated with primary antibody against PDGFRa (goat, 1:200, AF-307-NA, R&D Systems, MN, USA), CD90 (mouse ...
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bioRxiv - Immunology 2022Quote: ... cells were washed at day 7 and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 250 U/ml IFN-α (R&D Systems) ...
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bioRxiv - Developmental Biology 2022Quote: ... For the 2-day protocol in which cells were switched from Wnt3a to Activin A (R&D Systems, Minneapolis, MN), the samples were washed with PBS +/+ before adding fresh medium ...
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bioRxiv - Microbiology 2022Quote: ... The cells were cultured with 1000 U/mL human type I IFN-α2a (MφIFN; R&D Systems, Minneapolis, Minnesota, USA) or left untreated for 16 h.
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bioRxiv - Immunology 2022Quote: ... and MC-38 cells were cultured in Dulbecco’s Modified Eagle’s Medium (ATCC) supplemented with 10% fetal bovine serum (FBS, R&D Systems), 100 U/mL penicillin (Thermo) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were subsequently supplemented every two days with 1.5% CMG and 5 ng/mL of RANKL (R&D Systems) to induce osteoclastogenesis.
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bioRxiv - Immunology 2022Quote: ... were purified from mouse spleens and lymph nodes by negative selection using a T-cell enrichment column (R&D Systems). For the generation of OTI or OTII T blast cells ...
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bioRxiv - Immunology 2019Quote: ... Transduced cells were selected for in the presence of 2 µg/ml puromycin (R&D Systems Inc, Minneapolis, MN, USA), live cells harvested by ficoll gradient and flow cytometry used to determine purity based on expression of eBFP indicative of transduction.
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bioRxiv - Cancer Biology 2019Quote: All the cells used in this study were tested for mycoplasma using Mycoprobe Mycoplasma Detection kit from R&D system. Cells were passaged no more than 10 times from the initial stocks ...
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bioRxiv - Cell Biology 2019Quote: ... Directional cell migration was induced by overlaying the polymerized gels with 0.63μg/ml CCL19 diluted in complete media (R&D Systems). To prevent drying-out of the gels ...
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bioRxiv - Bioengineering 2020Quote: ... the cell culture medium was replaced by adipocyte differentiation medium or osteoblast differentiation medium (StemX-Vivo™, R&D systems). MSC expansion medium was also used as the control condition ...
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bioRxiv - Immunology 2019Quote: ... cells were simulated in R-10 medium in the presence of either 20 ng/mL IL-23 (R&D Systems) or 250 ng/mL PMA (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... IL-1β and IL-10 were measured in cell supernatants using an enzyme-linked immunosorbent assay technique (R&D systems). The sensitivity of the assays was (pg/ml ...
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bioRxiv - Immunology 2020Quote: ... IL-2 production was determined 24 h after T-cell activation by ELISA according to manufacturer’s instructions (R&D Systems). CD69 production was analyzed by flow cytometry after 2 days of anti-CD3 and/or anti-CD28 stimulation.
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bioRxiv - Immunology 2020Quote: Concentrations of IL-6 in cell culture supernatant were determined using the mouse DuoSet kit (R&D Systems, Minneapolis, MN). All assays were performed according the manufacturer’s supplied protocol ...
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bioRxiv - Immunology 2021Quote: IL-6 secretion from RAW264.7 cells was measured using the mouse IL-6 Duoset ELISA kit (R&D systems, UK) according to manufacturer’s procedure.
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bioRxiv - Cell Biology 2022Quote: IL-1β in mice serum and cell-free supernatants were measured by Quantikine® sandwich ELISA kits (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... immune mediators in cell supernatants were measured using Human Custom 29-plex Multi-Analyte Kit (R&D Systems, Minneapolis, Minnesota) measuring the following analytes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were stimulated with a pro-inflammatory cocktail of 20ng/ml interferon-γ (IFNγ, R&D Systems #485-MI-100) and 50ng/ml tumor necrosis factor α (TNFα ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed for RNA analysis and supernatants were analysed for IL-6 content by ELISA (DuoSet, R&D Systems) according to manufacturer’s instructions.