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Citations for R&D Systems :
351 - 400 of 594 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... NHEKs were induced to differentiate by addition of 1.2 mM CaCl2 to growth medium with or without addition of 1 μg/mL ephrin A1-FC peptide (R&D Systems) for the indicated times ...
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bioRxiv - Cell Biology 2021Quote: We performed a Phage-Fab (antigen-binding fragment) selection using a naïve Fab library (libF46) on an immobilized recombinant rat Unc5B-ECD Fc fusion protein (R&D systems). Phage particles fused with Fabs were incubated with an unrelated protein (e.g ...
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bioRxiv - Immunology 2022Quote: Cell surface staining for the anti-HER2 BCR was performed with Recombinant Human ErbB2/Her2 Fc Chimera Protein (R&D Systems) and APC anti-His Tag antibody (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... then stained in the presence of Fc block for 30 minutes at 4C using the following antibodies: CD4 (1:300; clone GK1.5; R&D Systems); CD44 (1:300 ...
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bioRxiv - Immunology 2021Quote: ... the same protein was labeled using its His-tag by incubating the antigen with an Alexa Fluor 647-conjugated anti-His-antibody (R&D Systems, IC0501R) for 30 minutes at room temperature at a 2:1 ratio (RBD molecules:IgG molecules) ...
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bioRxiv - Neuroscience 2021Quote: ... Western blotting was carried out using mouse monoclonal antibody clone M2 directed against the FLAG-tag on Npn2 (SigmaAldrich #F3165) or rabbit anti-NrCAM antibody (R&D Systems AF8538) and Avansta enhanced chemiluminescence ...
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bioRxiv - Cell Biology 2023Quote: ... 1 x 106 BM cells were incubated at 37°C or 4°C for 45 min with or without Cxcl12 (10 nM, R&D Systems). The reaction was stopped by adding ice-cold RPMI and brief centrifugation at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Release of cytochrome C from the mitochondria into the cytoplasm was assessed by a human cytochrome C Quantikine ELISA kit (Bio-techne R&D Systems) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... or control antibody (AB-108-C, R&D Systems), followed by exposure to human sera from normal ...
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bioRxiv - Cancer Biology 2023Quote: ... or IgG (Cat # AB-105-C, R&D Systems) -conjugated protein G Dynabeads (Cat # 10003D ...
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bioRxiv - Cancer Biology 2023Quote: ... c-REL (1:1,000, goat, R&D Systems #AF2699), c-REL (1:1,000 ...
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bioRxiv - Immunology 2019Quote: ... a suspension of 106 mononuclear cells in RPMI 1640 supplemented with 10% FCS was added to each insert in a well containing a solution of chemokine (R&D Systems). In some experiments ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then treated with 200ng/mL of recombinant rat NogoA (1026-1090aa) and Fc Chimera protein (Nogo-66; R&D Systems), or with human NogoA (566-748aa ...
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bioRxiv - Cell Biology 2021Quote: GFP-binding protein (GBP) (Rothbauer et al., 2008) was fused to the Fc domain of human IgG (pIg-Tail) (R&D Systems), tagged with His6 in pET28a (EMD Biosciences) ...
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bioRxiv - Biochemistry 2020Quote: In vitro co-precipitation of TREM2 or TREM1 with Aβ oligomers was performed by mixing recombinant Fc-tagged TREM2 or TREM1 ectodomain (residues 19-171; 100 ng/ml, R&D systems), 100 nM HiLyte Fluor 647-labelled Aβ oligomers and Protein G Dynabeads in 0.5% fatty acid free BSA PBS-Tween buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant proteins were separately preincubated with neuronal cultures and recombinant tau protein for 3 hours prior to incubation of neuronal cultures with the mixer containing recombinant proteins (100nM Human LRP-1 Cluster IV Fc Chimera Protein; 10nM Human LRPAP Protein [R&D systems]). For pharmacological treatments ...
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal 6xHis tag produced in HEK293 cells and human recombinant PD-L1-Fc chimera protein produced in mouse NS0 cells were obtained from R&D Systems, Minneapolis ...
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bioRxiv - Developmental Biology 2021Quote: ... were cultured on gelatine and FCS-coated plates in N2B27 medium supplemented with recombinant 20 ng ml–1activin A (338-AC-050, R&D Systems) and 2 μM IWP2 (3533 ...
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bioRxiv - Cell Biology 2019Quote: GFP-binding protein (GBP) (Rothbauer et al., 2008) was fused to the Fc domain of human IgG (pIg-Tail) (R&D Systems), tagged with His6 in pET28a (EMD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Cancer Biology 2023Quote: We assessed binding to a library of 300 glycan structures for Fc fusion proteins sourced from a commercial vendor (R&D Systems) composed of a fusion of Siglec-3 ...
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bioRxiv - Immunology 2023Quote: ... KIR2DL3*001 (#2014-KR-050) and KIR2DS4*001 (#1847-KR-050) KIR-Ig (KIR-Fc) fusion proteins were purchased from R&D systems. KIR2DS1*001-Fc was custom produced by Sino Biological ...
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bioRxiv - Neuroscience 2023Quote: ... co-cultures were maintained for 24 hours in the control condition or in the presence of the chimera proteins Fc-LGR4 (8077-GP, R&D Systems), Fc-LGR5 (8078-GP ...
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bioRxiv - Bioengineering 2024Quote: ... CAR T cells for the initial challenge day 0 imaging experiment were stained with the following antibody mix in FACS buffer (PBS 1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... cells from re-challenge plates were stained with the following antibody mix in FACS buffer (PBS1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins tagged with the TAP (tandem affinity purification) or HA (hemagglutinin) tag were detected with PAP or an anti-HA antibody (Sigma, Taufkirchen, Germany; R&D Systems, Minneapolis, USA) and a horseradish peroxidase (HRP)-coupled secondary antibody and CheLuminate-HRP ECL solution (Applichem ...
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bioRxiv - Biochemistry 2024Quote: ... Confirmation of ETB gene expression was confirmed in cell populations by surface staining with antibodies (anti-HA tag AlexaFluor 488 conjugated mouse IgG, R&D Systems, cat # IC6875G) in combination with flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Plasma C-reactive protein levels were assessed by a Mouse C-Reactive Protein/CRP Quantikine ELISA Kit (R&D Systems, Inc., MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... Serum Cystatin C levels were measured using the Mouse/Rat Cystatin C Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, MSCTC0). Blood Nitrogen Urea serum levels were measured using the QuantiChrom Urea Assay Kit (BioAssay Systems ...
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bioRxiv - Neuroscience 2021Quote: ... Nrp1-Fc fusion protein containing the extracellular domain of rat Nrp1 (amino acid 22-854 minus 811-828; R&D Systems, 566-NNS) or the control human Fc dimer (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... 100 µl of supernatant samples and standards (recombinant mouse TREM2-FC, 1729-T2; R&D Systems; 0-20 ng/ml in BSA-PBST) were then incubated for 2 hr at RT ...
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bioRxiv - Neuroscience 2021Quote: ... The contribution of BDNF/TrkB signaling was tested by bath application of a scavenger of endogenous BDNF (recombinant human TrkB-Fc chimera, R&D Systems, Germany). For scavenging of BDNF ...
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bioRxiv - Cancer Biology 2021Quote: ... Fc Tag 15μg/ml (CDO-H5257, Acro Biosystem Newark, USA) or Recombinant Human IgG1 Fc 3μg/ml (110-HG, R&D Systems, Minneapolis, MN, USA) as control ...
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bioRxiv - Immunology 2021Quote: The expression of NKG2D ligands in tumor cell lines was assessed using Recombinant Human NKG2D Fc Chimera Protein (R&D systems, Abingdon, UK). 105 tumor cells were incubated either with 0.5 μg of NKG2D Fc recombinant protein or IgG1-Fc during 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Conditioned media was collected from L-Wnt3a cells (ATCC CRL-2647™) and Cultrex HA-R-Spondin1-Fc 293T cells (R&D Systems), and the final organoid media formulation contained 20% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... or polyclonal rabbit IgG (R&D Systems, AB-105-C). In all analyses ...
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bioRxiv - Neuroscience 2020Quote: ... or Goat IgG control (AB-108-C, R&D Systems) was incubated right after slice cutting and perfused throughout recordings ...
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bioRxiv - Neuroscience 2019Quote: ... IgG (AB-105-C, R&D Systems, Minneapolis, MN, USA) or vehicle (sterile water ...
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bioRxiv - Microbiology 2023Quote: ... or isotype-matched antibody (#AB-108-C, R&D systems) was incubated at 0.2 µg/106 cells for ACE2-A549 and at 0.4 µg/106 cells for Vero E6 cells during 30 minutes at room temperature in 2% PBS-BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... or anti-IgG (Cat # AB-105-C, R&D Systems) antibody with mouse anti-SMARCD2 (Cat # sc-101162 ...
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bioRxiv - Bioengineering 2023Quote: ... and 3 µM CHIR99021 (C) (R&D Systems, 4423/50).
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bioRxiv - Cancer Biology 2023Quote: ... or goat anti-IgG (R&D Systems #AB-1080-C) as a control antibody in the relevant reduced serum media ...
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bioRxiv - Molecular Biology 2023Quote: ... The cytochrome c concentrations in the isolated cytosolic fraction were measured by rat/mouse cytochrome c quantikine ELISA Kit (MCTC0, R&D Systems, Inc., Minneapolis, MN).
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bioRxiv - Biophysics 2021Quote: ... supplemented with 0.3 μg Cholera Toxin Subunit B labeled with Alexa Fluor 555 (V-34404, Vybrant) and 50 nM dimeric EphrinA1-Fc (R&D Systems, #6417-A1). Control experiments were performed without ephrinA1-Fc ...
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bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
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bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
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bioRxiv - Microbiology 2021Quote: ... S1-Fc (Catalog # 10623-CV, lot DONK0120091) and S1-biotin (Catalog # BT10569, lot DOJW120092) were purchased from R&D Systems (Vendor #3; Minneapolis, MN). Streptavidin (Catalog# 85878-10MG ...
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bioRxiv - Cancer Biology 2022Quote: ... miniloci in an endogenous IgH and Igκ knockout background (19,20) were immunized with recombinant human LAG-3–Fc protein (hLAG-3–hFc) (R&D Systems, Minneapolis, MN, USA), consisting of the extracellular domain of LAG-3 (Leu23-Leu450 ...
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bioRxiv - Cancer Biology 2022Quote: ... +/-1 µg/mL anti-CD28 (CD28.2) antibody (eBioscience/Thermo 16-0289-81) or recombinant human CD58-Fc chimera protein (R&D Systems 10068-CD-050) at 37 ºC for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 1 μg/mL of either human rPD-1 (hrPD-1-Fc; R&D Systems #1086-PD-050; Minneapolis, MN, USA) or mouse rPD-1-Fc (R&D Systems #1021-PD-100 ...