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Citations for R&D Systems :
1 - 50 of 662 citations for Corning Lymphocyte Serum Free Medium since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... replaced the medium with serum-free DMEM/F12 medium containing N-2 supplement (R&D system), Leukemia Inhibitory Factor (LIF ...
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bioRxiv - Cancer Biology 2024Quote: ... The lower compartment contained 500 μl of either cancer cell line derived conditioned medium or control media (serum free medium; 2% serum control; CCL19 (20 ng/mL, R&D Systems). The PBMCs were allowed to migrate from the upper chamber to the lower one for 3 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stimulated overnight with 5 pg/ml of BMP9 in serum-free medium (R&D Systems) and luciferase activity was measured with the twinlite Firefly and Renilla Luciferase Reporter Gene Assay System (PerkinElmer) ...
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bioRxiv - Pathology 2022Quote: ... was seeded with 10 000 myoblasts in serum-free medium while the wells were filled with either proliferating medium or serum-free medium complemented with 10 ng.mL-1 FGF-b (R&D Systems 3139-FB), IGF-I (R&D Systems 791-MG ...
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bioRxiv - Cancer Biology 2021Quote: ... culture media were changed to serum free media or serum free media with 2 ug/ml IGF2 antibody (AF-292, R&D Systems) for another 72 hours ...
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bioRxiv - Biochemistry 2021Quote: ... the media were replaced with serum-free medium containing 10 μg/ml normal goat IgG or LDLR Ab (R&D Systems) in the continued presence of 0 or 100 ng/ml TNFα for 0 ...
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bioRxiv - Cell Biology 2020Quote: ... while 29-13 cells were passaged in Beck’s Medium supplemented with 15% doxycycline-free fetal calf serum (R&D Systems—Minneapolis, MN), 50 μg mL-1 hygromycin (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were kept in serum-free medium for 4 hours prior to stimulation and then incubated with recombinant CSF-1 or IL-34 (R&D Systems) for the indicated time points ...
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bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were starved in a serum-free high pH medium and then incubated overnight with a cocktail of 10 ng/ml TNFα (R&D Systems, 210-TA-100/CF) and 50 ng/ml IL-17A (R&D Systems ...
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bioRxiv - Genomics 2023Quote: ... EpiLCs were induced in N2B27 serum-free medium without 2i and LIF and with 40 μg/mL of BSA and 10 ng/ mL bFGF (R&D Systems, Cat. No 3139-FB-025) for 72 h ...
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bioRxiv - Cancer Biology 2021Quote: ... bottom chambers were filled with 500 μL of serum-free DMEM with chemoattractants (R&D Systems). Cells were cultured overnight at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2022Quote: ... some cultures were conducted in N2 minimum medium without serum (R&D System) and recombinant CXCL12 protein (R&D System ...
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bioRxiv - Bioengineering 2023Quote: ... The medium was supplemented with 10% fetal bovine serum (FBS, R&D Systems), 1% Penicillin/Streptomycin (Corning ...
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bioRxiv - Cell Biology 2021Quote: LECs were pre-cultured with FBS-free and supplement-free basal medium for 24 h and then treated with medium containing 100 ng/mL of recombinant rat CTGF (R&D Systems) for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Both mediums were supplemented with 10% tetracycline-depleted fetal calf serum (R&D Systems), 1000 U/ml of penicillin ...
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bioRxiv - Neuroscience 2024Quote: ... media change occurred using Neurobasal medium with 5% FBS (fetal bovine serum, R&D Systems S11550) containing growth factors 10 ng/ml of BDNF (Peprotech ...
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bioRxiv - Cancer Biology 2020Quote: ... Prostate-specific serum-free culture media contained 500 ng/mL human recombinant R-spondin (R&D Systems, Cat: 10820-904), 10uM SB202190 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: IL-1β in mice serum and cell-free supernatants were measured by Quantikine® sandwich ELISA kits (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the endodermal cells were dissociated into single cells using Accutase and plated on the Matrigel-coated plates in serum-free differentiation media (SFM) supplemented with 1.5 μM Dorsomorphin dihydrochloride (R&D System) and 10 μM SB431542 for 24h ...
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bioRxiv - Cell Biology 2021Quote: ... 1% (v/v) B27 Serum-Free Supplement (50x) (Life Technology, 17504044) and 50 ng/ml FGF7 (R&D System, 251-KG). For posterior foregut stage (d7-d8) ...
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bioRxiv - Developmental Biology 2024Quote: ... S7 medium was modified by supplementing it with 1 x N21-MAX insulin-free supplement (R&D SYSTEMS, AR010) during S7 (S7D1-S7D14).
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bioRxiv - Cell Biology 2022Quote: ... The floating cells were collected by centrifugation and the cells were resuspended in microglia media (DMEM/F12 + 1X Glutamax + 1X Sodium pyruvate + 0.1% of fatty acid-free bovine serum albumin and 1X penicillin/streptomycin + 1X ITS supplement (R&D Systems AR013) + 25 ng/mL M-CSF (GoldBio 1320-09-10) ...
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bioRxiv - Cell Biology 2020Quote: ... B-27 Serum-Free Supplement (50x) (Cat. No. 17504044, Life Technology) + 50 ng/ml FGF7 (Cat. No. 251-KG, R&D System) for 2 days ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in low cluster plates to allow EB formation in serum-free differentiation SFD media supplemented with BMP4 (3 ng/ml, R&D Systems). At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml ...
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bioRxiv - Systems Biology 2024Quote: ... before being incubated with serum-free DMEM with Recombinant Mouse GAS6 protein (0.05– 1000ng/mL, R&D Systems, 986-GS-025/CF), Recombinant Mouse Protein S/PROS1 (100ng/mL ...
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bioRxiv - Systems Biology 2022Quote: ... Levels of lymphocyte-derived cytokines (IFNγ, IL-17) were determined after 7 days (PeliKine Compact or Duoset ELISA, R&D Systems). Baseline inflammatory plasma markers from both cohorts ...
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bioRxiv - Physiology 2023Quote: ... ciCMVEC were placed in serum-free media 1h before treatment with 10 ng/ml of Recombinant Human TNF-α Protein (210-TA-005; R&D systems) or vehicle (PBS ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) containing 5% fetal bovine serum (FBS; R&D Systems), gentamicin (50 μg/mL) ...
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bioRxiv - Immunology 2021Quote: ... and TNF-a in cell culture medium and serum was measured using commercially available ELISA kits (R&D Systems). SARS-CoV-2 S protein in cell lysates was detected by SARS-CoV-2 S ELISA kit and following manufacturer’s instruction (RayBiotech ...
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bioRxiv - Immunology 2022Quote: CD4+ splenic T-lymphocytes were polarized to TH17 ex vivo by use of CellXVivo Mouse Th17 Cell Differentiation Kit (#CDK017, R&D Systems). Briefly ...
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bioRxiv - Immunology 2024Quote: ... ex vivo lamina propria lymphocytes were cultured for 20 h at 37°C in complete RPMI with IL-23 (25 ng ml−1 R&D Systems) and IL-1β (20 ng ml−1 Peprotech ...
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bioRxiv - Bioengineering 2020Quote: ... Microvascular fragment-containing gels were cultured in serum-free media supplemented with 10 ng/mL recombinant human vascular endothelial growth factor (rhVEGF; R&D Systems, Minneapolis, MN) (55) ...
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bioRxiv - Immunology 2022Quote: ... and MC-38 cells were cultured in Dulbecco’s Modified Eagle’s Medium (ATCC) supplemented with 10% fetal bovine serum (FBS, R&D Systems), 100 U/mL penicillin (Thermo) ...
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bioRxiv - Cell Biology 2024Quote: ... and HIRAfl/fl Cre-ERT MEF cells were prepared from E12.5 embryos and maintained in Dulbecco’s minimal essential medium (DMEM) supplemented with 10% fetal bovine serum (R&D systems) and 1% antibiotics (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... and BHK-21-hACE2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (R&D Systems) and 1% penicillin-streptomycin (P/S ...
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bioRxiv - Genomics 2020Quote: ... the EBs were dissociated with trypsin-EDTA and reaggregated for 40 hr in serum-free differentiation media with the addition of 8ng/ml human activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R & D Systems Cat# 314-BP) ...
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bioRxiv - Cancer Biology 2020Quote: ... Invasion was also quantified in response to serum-free DMEM supplemented with 10 ng/mL of recombinant mouse CSF2 (R&D Systems, 415-ML-5), PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s protocol and reaggregated for 40h in serum-free differentiation media with the addition of 8 ng/ml human Activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
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bioRxiv - Developmental Biology 2024Quote: ... the embryoid bodies (EB) were harvested and seeded in serum-free differentiation media supplemented with 1 ng/ml human Activin A (R&D Systems Cat#. 338-AC) and 1 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
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bioRxiv - Cell Biology 2020Quote: ... Fibers were incubated in plating medium consisting of 15% Fetal Bovine Serum (Hyclone) and 2 ng/ml bFGF (R&D Systems) in DMEM.
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bioRxiv - Pathology 2020Quote: ... cells of 2 MOI were treated with serum-free media for 24 hours and then media containing TGF-β (20 ng/ml, R&D Systems, Minneapolis, MN, USA) for 72 hours ...
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bioRxiv - Cell Biology 2022Quote: ... HSC cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA; for 3 d), the ROCK inhibitor Y27632 (10 μM ...
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bioRxiv - Immunology 2024Quote: Supernatants harvested from mixed lymphocyte cultures established from three mice of each group were tested using commercial assay kits (e.Bioscience, R&D Systems, Bender MD Systems) for the presence of interferon-γ ...
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bioRxiv - Immunology 2022Quote: ... monocytes were cultivated for 6 days with a change of medium at day 3 in RPMI 1640/10% human serum and 10 ng/ml recombinant M-CSF (R&D Systems). At day 6 the medium was replaced with RPMI 1640/10% human serum and used for experiments on day 7.
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bioRxiv - Cell Biology 2021Quote: ... Culture was continued for 24 h in low serum medium with 0 or 5 ng/ml TGF-β1 (R&D Systems, www.rndsystems.com) with emodin (0 ...
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Cell Biology 2023Quote: ... cells were maintained in Dulbecco’s modified Eagle medium (DMEM) (Wisent Inc., 319-005-CL) supplemented with 10% fetal bovine serum (FBS) (R&D Systems, S12450) and 1% Penicillin/Streptomycin (P/S ...
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bioRxiv - Developmental Biology 2021Quote: ... in serum-free media (DMEM/F12 supplemented with L-glutamine and penicillin-streptomycin) with added FGF ligands (Fgf7; 4 µM, R&D Systems, Cat. No. 5028-KG-025), the γ-secretase inhibitor DAPT (10 µM ...
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bioRxiv - Cell Biology 2021Quote: Recombinant mouse Wnt9b (carrier free) and Rspo3 (carrier free) was purchased from R&D systems. Wnt9b (25 μg ...