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Citations for R&D Systems :
501 - 550 of 741 citations for Chikungunya Virus VLP E1 E2 C protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: 0.3M of purified proteins (recombinant human E-cadherin 8505 and recombinant human EGFR 344-ER R&D system) were mixed and incubated in 100μl of lysis buffer (25 mM TrisHCl pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: EphrinB3 epitopes in myelin extract proteins were neutralized by incubation with anti-EphrinB3 antibodies (AF395, R&D system and sc-271328 ...
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bioRxiv - Biochemistry 2021Quote: ... Test wells were coated with 5 µg recombinant spike protein (rSP; S1+S2; R&D Systems, Minneapolis, MN)/ml coating buffer (0.05 M sodium bicarbonate ...
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bioRxiv - Developmental Biology 2021Quote: ... animals were incubated in 250 ng/mL recombinant zebrafish BMP4 protein (R&D Systems, cat. 1128-BM-010) in 0.08% bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2022Quote: ... followed by incubation with 0.01 mg/mL recombinant human VCAM-1/CD106 Fc chimera protein (R&D Systems) and 0.01 mg/mL ChromPure Human IgG ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins of interest were detected using the following primary antibodies: goat anti-TREM2 (R&D Systems, Inc., #AF1828), rabbit anti-PGRN (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: To assess the levels of hippocampal BDNF protein the BDNF Quantikine ELISA kit (R&D Systems, Wiesbaden, Germany) was used ...
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bioRxiv - Immunology 2020Quote: ... and CXCL12 protein concentration assayed with the Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit (R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... 50-100μg of protein lysate was treated for 6-8hrs with chABC (100 μU/mL; R&D systems) before being subjected to traditional western blotting ...
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bioRxiv - Cancer Biology 2022Quote: For recombinant E6 proteins and sdAbs: 2 µg of E6 (R&D Systems Inc., Cat. # AP-120-025), MBP (Abnova ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stained with directly conjugated antibodies against human proteins: alkaline phosphatase-APC (R&D Systems; RRID: AB_357039), CD73-PE (BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were used: SOX9 (1:50, AF3045, R&D Systems, and AB5535, Millipore), ERG (1:50 ...
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bioRxiv - Physiology 2021Quote: ... and mouse recombinant proteins of IL-6 and TNFα were brought from R&D system (Minneapolis, MN, USA). Mouse anti-eMyHC was purchased form Developmental Studies Hybridoma (DSHB ...
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bioRxiv - Immunology 2020Quote: ... Recombinant human MFG-E8 protein produced in mouse myeloma cells was purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Neuroscience 2022Quote: ... Protein levels were measured using the Proteome Profiler Human Cytokine Array Kit (R&D Systems product number ARY005B) as per the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL, R&D Systems). The readout was HRP-conjugated anti-His tag antibody recognizing the pentraxin proteins (abcam).
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bioRxiv - Immunology 2023Quote: Cytokine protein levels were determined either by ELISA (Enzyme-Linked ImmunoSorbent Assay) using kits from R&D Systems Inc ...
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bioRxiv - Pathology 2024Quote: ... Cell surface protein expression was performed by labeling 2×105 cells for KDR-PE (R&D Systems, FAB357P) + PDGFRα-APC (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: Recombinant Human Neuronal Pentraxin 1 Protein (NP1) was purchased from R&D systems (#: 7707-NP, bio-techne, USA).
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bioRxiv - Cell Biology 2023Quote: ... Levels of protein phosphorylation were then detected using the Human phospho-kinase Array Kit (ARY003B, R&D Systems) according to the manufacturer’s instructions (Table S3) ...
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bioRxiv - Immunology 2024Quote: ... Mouse and human ACE2 protein levels were quantified from lung homogenates by ELISA (R&D Systems #DY3437, #DY933).
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bioRxiv - Immunology 2024Quote: ... following reagents were used in flow cytometry: recombinant human PD-L1 Fc chimera Biotin protein (R&D Systems); APC Streptavidin (Tonbo Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... in PBS for 1LJh and then stained overnight at 4°C with a primary anti-SP-D antibody (1:500, R&D Systems, AF1920), diluted in PBS with 0.5% BSA and 0.5% Triton X-100 ...
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bioRxiv - Physiology 2019Quote: ... tissue from the wound area was harvested and stored at −80°C for analysis of VEGF using an enzyme-linked immunosorbent assay (murine VEGF-specific ELISA kit (R&D Systems, USA) and a BCA protein assay kit (Thermo scientific ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were then incubated overnight at 4°C in buffer G with the following antibodies: mouse monoclonal anti-Tuj1 (R&D Systems, MAB1195) at 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... immunosorbent 96-well plate was coated overnight at +4°C with 1 μg/ml of goat anti-mouse CDNF (R&D Systems, AF5187) in coating buffer (15mM sodium carbonate ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2, BD Bioscience; ARL13B, ProteinTech; IMPDH2, Abcam; SMO, Santa Cruz; and Gli1, R&D Systems) diluted with 1% BSA/0.3% Triton-X/PBS solution ...
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bioRxiv - Immunology 2021Quote: All cells were grown in a humidified incubator at 37°C and 5% CO2 and cell lines tested mycoplasma negative (MycoProbe® Mycoplasma Detection Kit, R&D systems).
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bioRxiv - Cancer Biology 2022Quote: ... 500 000 cells were stained for 20 min at 4°C in the dark with the APC-conjugated anti-P-cad antibody (1:10; R&D system, FAB861A) and the Live/DeadTM Fixable Violet Dead Cell Stain Kit (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... paraformaldehyde-fixed frozen sections were treated with citrate buffer (pH 7.0, 121°C, 5 min) before immunostaining with sheep anti-mouse Spi-B polyclonal Ab (R&D Systems, Abingdon, UK). CCL20 ...
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bioRxiv - Immunology 2022Quote: ... were incubated for 30 min at 20°C in 50 μl RPMI-HEPES in the absence of BCS with 2 μg/ml anti-ACE2 antibody (R&D Systems, #AF933) (Hoffmann et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for anti-mouse TREM2 biotinylated (R&D systems, BAF1729) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... LAD2 cells were prior-treated with 0.25% trypsin (without EDTA) for 10 min at 37 °C or prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 2 h at 37 °C before the incubation with Spike-RBD protein.
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in a humidified incubator at 37∘C and 5% CO2 and tested mycoplasma negative (MycoProbe® Mycoplasma Detection Kit, R&D systems). HEK293T cells ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were blocked in 3% bovine serum albumin for 30 min and incubated with the following primary antibodies overnight at 4°C: β-III tubulin (1:1000, MAB1195, R&D system), rabbit anti-MAP2 (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... the conditioned media was stored at -80° C and the interleukin-6 (IL-6) content was quantified via enzyme-linked immunosorbent assay (R&D Systems, DY406) following the manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: ... was applied for 30 minutes at room temperature followed by primary antibody staining overnight at 4°C using anti-CD206 (R&D systems, AF2534), anti-CD11b (Cell Sciences ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then sections were incubated overnight at 4 ◦C with goat polyclonal anti-S100a10 antibody (1:200; AF2377, R&D Systems, Minneapolis, MN, USA), rabbit polyclonal anti-5HT1B Receptor antibody (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant of BALF was store at -80°C for cytokine analysis by mouse TNF-α and IL-1β DuoSet ELISA kits (R&D Systems). Lungs were perfused with a 1:1 mixture of OCT and 4% PFA and preserved in OCT cryomolds (Tissue-Tek ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for 40 min at 4°C with fluorophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated overnight at 4°C with a goat anti-SAA primary antibody (1:500; AF2948, R&D Systems, Minneapolis, MN). This was followed by a 1 h room temperature incubation with a horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor lysates were quantified and 40 μg protein loaded for analysis of GzB levels by ELISA (R&D Systems).
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bioRxiv - Immunology 2019Quote: ... CXCL8 and Osteopontin (SPP1) proteins in conditioned media was performed using a custom human luminex assay (R&D systems), according to the manufacturers protocol ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 5000 RU of Recombinant Human Desmoglein-2 Fc Chimera Protein (R&D Systems, Catalogue number 947-DM-100) was amine coupled to a CM5 sensor chip at a slow flow-rate of 10 μl/min to ensure uniform distribution on the chip surface.
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bioRxiv - Molecular Biology 2019Quote: ... were pre-treated with 2ug/mL recombinant mouse Slit2 protein (gln26-gln900) (rSlit2) (R&D system, 5444-SL-050) or PBS for at least 18 hours before removing inserts ...
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bioRxiv - Bioengineering 2019Quote: ... and 0.005 N NaOH along with 10 ng/mL bone morphogenetic protein 4 (R&D Systems, Minneapolis, MN, USA). 4 × 105 cells were plated in each PDMS culture well with 3-mm pillar ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells surface ACE2 protein was stained with Alex Flour® 647-conjugated human ACE2 antibody (R&D Systems FAB933R). Live/Dead (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... one multiplex panel was used to measure fatty acid binding protein 4 (FABP4) and resistin (R&D Systems, UK); non-esterified fatty acid (NEFA ...
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bioRxiv - Immunology 2020Quote: ... anti-CD28 (1 µg/ml, clone CD28.2, eBioscience) and immobilized recombinant ICAM1-Fc fusion protein (0.5µg/ml, R&D Systems) in complete RPMI/HS medium ...
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bioRxiv - Immunology 2022Quote: ... S proteins used in the study were SARS-CoV-2 S GCN4-IZ-2P (10561-CV, R&D Systems), SARS-CoV-2 B.1.1.529 S GCN4-IZ-2P (11061-CV ...