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Citations for R&D Systems :
201 - 250 of 259 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat ...
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bioRxiv - Microbiology 2023Quote: ... separated and blotted onto 0.2 μm nitrocellulose as above and incubated overnight at 4°C in blocking buffer supplemented with 2 μg/ml polyclonal sheep IgG anti-LL-37 (R&D Systems). Membranes were washed in wash buffer (PBS with 0.05% Tween ...
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bioRxiv - Cell Biology 2023Quote: ... Media for the ELISA was immediately stored after collection at -80°C for subsequent testing in the enzyme-linked immunoassay (R&D systems). The Human IL-1β Quantikine ELISA Kit (R&D Systems cat ...
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bioRxiv - Biochemistry 2023Quote: ... plates incubated with biotinylated antibody were washed and incubated at 37°C for 1 hr with a 1/200 dilution of Streptavidin-HRP (Catalog #890803, R&D systems). Plates were then washed and developed using 100□μl/well SureBlue TMB (3,3′ ...
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bioRxiv - Immunology 2023Quote: ... Sorted cells were plated at 5000 cells per well in a 96-well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no. 39649, lot 02617020, used 1:250; SOX2, R&D Systems, AF2018 ...
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bioRxiv - Immunology 2023Quote: ... The culture was then diluted 1:50 in 1X PBS + 1% LB broth and incubated statically at 37°C with recombinant human SLPI protein (rSLPI) (CAS: 1274-PI-100, R&D Systems) at these final concentrations ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated overnight at 4°C in blocking buffer with anti-E-cadherin (R&D Systems; Minneapolis, MN, USA; cat:MAB1838), anti-integrin-⍺-6 (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies: sheep anti-THP polyclonal antibody (1:40, catalog no. AF5175; R&D Systems), goat anti-MPO polyclonal antibody (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... in PBS for 1LJh and then stained overnight at 4°C with a primary anti-SP-D antibody (1:500, R&D Systems, AF1920), diluted in PBS with 0.5% BSA and 0.5% Triton X-100 ...
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bioRxiv - Physiology 2019Quote: ... tissue from the wound area was harvested and stored at −80°C for analysis of VEGF using an enzyme-linked immunosorbent assay (murine VEGF-specific ELISA kit (R&D Systems, USA) and a BCA protein assay kit (Thermo scientific ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were then incubated overnight at 4°C in buffer G with the following antibodies: mouse monoclonal anti-Tuj1 (R&D Systems, MAB1195) at 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... immunosorbent 96-well plate was coated overnight at +4°C with 1 μg/ml of goat anti-mouse CDNF (R&D Systems, AF5187) in coating buffer (15mM sodium carbonate ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2, BD Bioscience; ARL13B, ProteinTech; IMPDH2, Abcam; SMO, Santa Cruz; and Gli1, R&D Systems) diluted with 1% BSA/0.3% Triton-X/PBS solution ...
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bioRxiv - Immunology 2021Quote: All cells were grown in a humidified incubator at 37°C and 5% CO2 and cell lines tested mycoplasma negative (MycoProbe® Mycoplasma Detection Kit, R&D systems).
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bioRxiv - Cancer Biology 2022Quote: ... 500 000 cells were stained for 20 min at 4°C in the dark with the APC-conjugated anti-P-cad antibody (1:10; R&D system, FAB861A) and the Live/DeadTM Fixable Violet Dead Cell Stain Kit (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... paraformaldehyde-fixed frozen sections were treated with citrate buffer (pH 7.0, 121°C, 5 min) before immunostaining with sheep anti-mouse Spi-B polyclonal Ab (R&D Systems, Abingdon, UK). CCL20 ...
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bioRxiv - Immunology 2022Quote: ... were incubated for 30 min at 20°C in 50 μl RPMI-HEPES in the absence of BCS with 2 μg/ml anti-ACE2 antibody (R&D Systems, #AF933) (Hoffmann et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for anti-mouse TREM2 biotinylated (R&D systems, BAF1729) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... LAD2 cells were prior-treated with 0.25% trypsin (without EDTA) for 10 min at 37 °C or prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 2 h at 37 °C before the incubation with Spike-RBD protein.
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bioRxiv - Microbiology 2021Quote: Sterile tissue culture plates were coated overnight at 4°C with 5 µg/mL recombinant CD62P protein (R&D Systems, #137-PS-050). The following day the wells were washed 3X with PBS before being blocked with 1% BSA in PBS at room temperature (RT ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in a humidified incubator at 37∘C and 5% CO2 and tested mycoplasma negative (MycoProbe® Mycoplasma Detection Kit, R&D systems). HEK293T cells ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were blocked in 3% bovine serum albumin for 30 min and incubated with the following primary antibodies overnight at 4°C: β-III tubulin (1:1000, MAB1195, R&D system), rabbit anti-MAP2 (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... the conditioned media was stored at -80° C and the interleukin-6 (IL-6) content was quantified via enzyme-linked immunosorbent assay (R&D Systems, DY406) following the manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: ... was applied for 30 minutes at room temperature followed by primary antibody staining overnight at 4°C using anti-CD206 (R&D systems, AF2534), anti-CD11b (Cell Sciences ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then sections were incubated overnight at 4 ◦C with goat polyclonal anti-S100a10 antibody (1:200; AF2377, R&D Systems, Minneapolis, MN, USA), rabbit polyclonal anti-5HT1B Receptor antibody (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant of BALF was store at -80°C for cytokine analysis by mouse TNF-α and IL-1β DuoSet ELISA kits (R&D Systems). Lungs were perfused with a 1:1 mixture of OCT and 4% PFA and preserved in OCT cryomolds (Tissue-Tek ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for 40 min at 4°C with fluorophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated overnight at 4°C with a goat anti-SAA primary antibody (1:500; AF2948, R&D Systems, Minneapolis, MN). This was followed by a 1 h room temperature incubation with a horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: ... for 24 hours and rested overnight in C-RPMI with 100µM ferric ammonium citrate (FeAC) and 200U/ml of human recombinant IFN-γ (R&D Systems, MN USA) unless otherwise stated.
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bioRxiv - Neuroscience 2021Quote: ... every third sagittal section of tissue containing the left fastigial nucleus was blocked with 5% bovine serum albumin for one hour at room temperature prior to incubation in primary antibody goat anti-osteopontin overnight at 4°C (1:300, R&D Systems AF808; RRID:AB_2194992). Tissue was then rinsed and incubated in donkey anti-goat Alexa Fluor 594 for 2 hours at room temperature (1:500 ...
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bioRxiv - Immunology 2019Quote: ... sections were incubated overnight at 4°C with an antibody mix containing rat anti-mouse IL-18 (5 μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887], α-Snail [R&D Systems AF3639] ...
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bioRxiv - Immunology 2020Quote: Macrophages were centrifuged for 7 minutes at 1200 rpm and then stained for 40 minutes at 4°C with fluor ophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Genomics 2020Quote: Plasma was used to assess circulating monocyte chemoattractant protein (MCP)-1 and C-reactive protein (CRP) via ELISA following manufacturer’s instructions (R&D Systems, Minneapolis, MN, USA) (1).
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation with the appropriate primary antibodies in blocking solution at 4°C overnight (1:1000 rabbit anti-IBA1 (FujiFilm Wako Chemicals, Cat # 019-19741) & 1:500 sheep anti-TREM2 (R&D Systems, Cat # AF1729)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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bioRxiv - Bioengineering 2020Quote: ... The slides were then incubated overnight in the dark at 4°C with primary antibodies against: Nephrin (NPHS1,AF4269, R&D Systems, 1:300), Lotus Tetragonolobud Lectin (LTL ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were transferred to a membrane using the iBlot2 system and incubated overnight at 4°C with primary antibody (anti-Gli3, 1:500, AF3690, R&D Systems, Minneapolis, MN). Membranes were then incubated in secondary antibody (anti-goat Alexafluor 680 ...
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bioRxiv - Immunology 2020Quote: ... cells were also incubated overnight at 4°C with a sheep anti-Lamp1 Alexa Fluor 488-conjugated (lysosomal marker) antibody (R&D systems, Cat# IC7985G) at 1:10 or a rabbit anti-CD71 (transferrin receptor ...
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bioRxiv - Microbiology 2023Quote: ... Huiskonen (University of Oxford) and incubated for three days at 37 °C in the presence of 1 μg/ml of kifunensine (R&D Systems, MN, USA). Supernatant was collected and combined with half a volume of binding buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... female BALB/c mice were anaesthetized and injected as described above with 1 μg CINC-1 protein (453-KC-050/CF, R&D systems, Abingdon, UK) in 1 μL saline in the left striatum ...
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bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
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bioRxiv - Neuroscience 2024Quote: ... double-coated with PLO as described above followed by a second coating with fibronectin (5µg/ml, incubation overnight at 37 °C; R&D Systems, Minneapolis, MN, USA) and once washing with PBS.
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Microbiology 2020Quote: ... Caco2 cells were pre-treated for 30 min at 37°C with goat antibody directed against the human ACE2 or DDP4 ectodomain (R&D Systems, Wiesbaden-Nordenstadt, Germany). Then ...
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bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...