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Citations for R&D Systems :
501 - 550 of 773 citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... or differentiation medium with tamoxifen and 10µg/mL of soluble fibronectin protein (R&D systems, 1918-FN). Images were analyzed using QuPath v0.4.0 (52).
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated over night at 4 °C with the following antibodies diluted in PBS 2.5% BSA: goat anti-CD140a 1:80 (R&D Systems AF1062) and rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S) ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Immunology 2019Quote: ... and resuspended for culture at 37°C + 5% CO2 for 1 h prior to stimulation with recombinant human TNFα (R&D Systems). Following stimulation ...
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bioRxiv - Neuroscience 2020Quote: ... and 10mM citrate buffer pH 6.0 at 95°C x 5 mins for Prox1 (neutral buffer CTS016, R&D Systems, Minneapolis, MN). Sections were then blocked with 10% or 5% (for Tbr2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pH 7.6] for 2 h at RT and incubated overnight (ON) at 4°C with antibodies against TRKB (1:1000, #AF1494, R&D Systems); AP2M (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated with a primary antibody cocktail containing one or more of the following antibodies overnight at 4°C: goat anti-mouse NKp46 (1:100, R&D Systems), anti-mouse CD3e-AF594 or AF647 (500A2 ...
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bioRxiv - Neuroscience 2020Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were blocked with 5% normal donkey serum followed by overnight incubation at 4 °C using the following mixture of primary antibodies: goat anti-IL-1β/ILF2 (1:250; #AF-401-NA, R&D Systems), rat anti-CD45 (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked with blocking buffer for 30 min at room temperature and followed by incubation with primary antibody overnight at 4°C (all primary antibodies used for dot blot were purchased form, R&D systems; anti-Cathepsin A ...
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bioRxiv - Microbiology 2020Quote: Cells were stimulated for 16 h prior to infection in complete medium at 37°C with addition of 50 IU/mL human IFNγ (285-IF, R&D Systems). Induction of Doxycycline-inducible cells was performed with 200 ng/mL Doxycycline overnight (D9891 ...
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bioRxiv - Cell Biology 2020Quote: ... serum starved at 37°C in MV2 medium (containing no growth factors) 3 hrs before stimulation with VEGFA164 (50 ng/ml; R&D Systems), followed by fixation in 3% PFA for 3 min ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids were maintained at 37°C and 5% v/v CO2 in Basement Membrane Extract (BME) (R&D Systems, Abingdon, UK) and cultured in expansion medium (ADMEM/F12 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Microbiology 2022Quote: ... followed by three days incubation at 37 °C in the presence of 1 µg/ml of kifunensine (R&D Systems, MN, USA). Supernatant was collected and combined with half volumes of the supernatant of binding buffer (25 mM HEPES ...
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bioRxiv - Immunology 2020Quote: ... 1.25% of supernatant from Con-A-stimulated rat splenocytes (assay positive control), c) recombinant canine IL-4 (rcaIL-4, 360 ng/mL) (R&D Systems), d ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies were immobilized on CM5-Sepharose chips coated by standard amine coupling chemistry to a density of about 600 response units (RU) Measurements were carried out at 25°C by injecting 250 μl of recombinant human TNFa (R&D Systems) at concentrations from 0.2 to 50 nM with a flow rate of 35μl/min ...
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bioRxiv - Biochemistry 2021Quote: Human CD38 (P28907, a soluble fragment Val43-Ile300 with 6x-His tag at C terminus) was purchased from R&D Systems (USA). It was diluted to final 0.01 mg/ml in 100 mM HEPES/NaOH buffer ...
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bioRxiv - Cell Biology 2022Quote: ... freshly isolated RBCs in basal medium supplemented with 10 mM HEPES at 20% hematocrit were incubated for 30 minutes under 37 °C with gentle shaking with 5 μg/mL ADAMTS13 (R&D Systems) [27 ...
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bioRxiv - Biophysics 2022Quote: ... ECD fragments (modified C-terminally with 10xHis tag) Podocalyxin (PODXL) (Cat.# 1658-PD) and PODXL2 (Cat.# 1524-EG) were obtained from R&D Systems. The ECD fragments were dissolved in ultrapure water to a concentration of 0.5 mg/mL and aliquoted in 50 μL ...
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bioRxiv - Cell Biology 2019Quote: ... slides were blocked and stained using rabbit anti-LOXL2 (Gil25-70, Gilead Sciences) antibody overnight at 4° C and a rabbit cell & tissue staining kit (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested and stocked at −20°C until CXCL8 quantification by ELISA kits according the instructions of the manufacturer (R&D system).
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked in 5% nonfat dry milk in TBST and probed overnight at 4°C with antibodies recognizing ERAP1 (AF2334, R&D Systems), ERAP2 (AF3830 ...
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bioRxiv - Immunology 2022Quote: ... Sorted cells were plated at 5000 cells per well in a 96 well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
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bioRxiv - Neuroscience 2022Quote: ... The pre-immobilized beads were then incubated with 50 nM recombinant mouse SorCS1 ectodomain tagged with a C-terminal 6-His tag (SorCS1-His, Cat# 4395-SR-050, R&D systems) in ECS for 2 h at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... Media for the ELISA was immediately stored after collection at -80°C for subsequent testing in the enzyme-linked immunoassay (R&D systems). The Human IL-1β Quantikine ELISA Kit (R&D Systems cat ...
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bioRxiv - Biochemistry 2023Quote: ... plates incubated with biotinylated antibody were washed and incubated at 37°C for 1 hr with a 1/200 dilution of Streptavidin-HRP (Catalog #890803, R&D systems). Plates were then washed and developed using 100□μl/well SureBlue TMB (3,3′ ...
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bioRxiv - Immunology 2023Quote: ... Sorted cells were plated at 5000 cells per well in a 96-well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no. 39649, lot 02617020, used 1:250; SOX2, R&D Systems, AF2018 ...
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bioRxiv - Biophysics 2024Quote: Recombinant sKLA (Glu34-Ser981) with a C-terminal 6His tag expressed in a NS0 murine myeloma cell line was purchased (R&D Systems) and loaded onto a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies: sheep anti-THP polyclonal antibody (1:40, catalog no. AF5175; R&D Systems), goat anti-MPO polyclonal antibody (1:200 ...
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bioRxiv - Cell Biology 2020Quote: 0.3M of purified proteins (recombinant human E-cadherin 8505 and recombinant human EGFR 344-ER R&D system) were mixed and incubated in 100μl of lysis buffer (25 mM TrisHCl pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: EphrinB3 epitopes in myelin extract proteins were neutralized by incubation with anti-EphrinB3 antibodies (AF395, R&D system and sc-271328 ...
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bioRxiv - Biochemistry 2021Quote: ... Test wells were coated with 5 µg recombinant spike protein (rSP; S1+S2; R&D Systems, Minneapolis, MN)/ml coating buffer (0.05 M sodium bicarbonate ...
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bioRxiv - Developmental Biology 2021Quote: ... animals were incubated in 250 ng/mL recombinant zebrafish BMP4 protein (R&D Systems, cat. 1128-BM-010) in 0.08% bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2022Quote: ... followed by incubation with 0.01 mg/mL recombinant human VCAM-1/CD106 Fc chimera protein (R&D Systems) and 0.01 mg/mL ChromPure Human IgG ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins of interest were detected using the following primary antibodies: goat anti-TREM2 (R&D Systems, Inc., #AF1828), rabbit anti-PGRN (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: To assess the levels of hippocampal BDNF protein the BDNF Quantikine ELISA kit (R&D Systems, Wiesbaden, Germany) was used ...
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bioRxiv - Immunology 2020Quote: ... and CXCL12 protein concentration assayed with the Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit (R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... 50-100μg of protein lysate was treated for 6-8hrs with chABC (100 μU/mL; R&D systems) before being subjected to traditional western blotting ...
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bioRxiv - Cancer Biology 2022Quote: For recombinant E6 proteins and sdAbs: 2 µg of E6 (R&D Systems Inc., Cat. # AP-120-025), MBP (Abnova ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stained with directly conjugated antibodies against human proteins: alkaline phosphatase-APC (R&D Systems; RRID: AB_357039), CD73-PE (BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were used: SOX9 (1:50, AF3045, R&D Systems, and AB5535, Millipore), ERG (1:50 ...
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bioRxiv - Physiology 2021Quote: ... and mouse recombinant proteins of IL-6 and TNFα were brought from R&D system (Minneapolis, MN, USA). Mouse anti-eMyHC was purchased form Developmental Studies Hybridoma (DSHB ...
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bioRxiv - Immunology 2020Quote: ... Recombinant human MFG-E8 protein produced in mouse myeloma cells was purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Neuroscience 2022Quote: ... Protein levels were measured using the Proteome Profiler Human Cytokine Array Kit (R&D Systems product number ARY005B) as per the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL, R&D Systems). The readout was HRP-conjugated anti-His tag antibody recognizing the pentraxin proteins (abcam).
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bioRxiv - Immunology 2023Quote: Cytokine protein levels were determined either by ELISA (Enzyme-Linked ImmunoSorbent Assay) using kits from R&D Systems Inc ...