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Citations for R&D Systems :
451 - 500 of 3416 citations for 7H Purine 7 acetamide N dibenzofuran 3 yl 1 2 3 6 tetrahydro 1 3 dimethyl 2 6 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... to RAW264.7 or 2 ng/mL transforming growth factor β-1(240-B/CF, R&D System, USA) to THP-1 for 24-36 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... Control or shLATS1/2 cells were counted and resuspended in 1% methylcellulose medium (R&D system Cat. # HSC001) prepared in complete growth medium ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Next day the plates were washed 3 times and the color development was performed using Duoset ELISA Development System (DY999, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... ROCK inhibitor was removed from the medium 3 hours after seeding and cells were induced the following day with 50 ng/ml BMP4 (R&D Systems), 2 μM IWP2 (Stemgent) ...
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bioRxiv - Immunology 2022Quote: ... monocytes were cultivated for 6 days with a change of medium at day 3 in RPMI 1640/10% human serum and 10 ng/ml recombinant M-CSF (R&D Systems). At day 6 the medium was replaced with RPMI 1640/10% human serum and used for experiments on day 7.
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bioRxiv - Immunology 2020Quote: ... supernatants were collected and secretion of TGF β and MMP2/3/9 was assessed in the supernatant by TGFβ1 ELISA kit (R&D systems) or MMP2/3/9 luminex kit (eBiosciences ...
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bioRxiv - Microbiology 2020Quote: ... hESCs were cultured to achieve 80-90% confluency, and treated with 3 μM CHIR99021 (CHIR, Stem-RD) and 100 ng/ml Activin A (R&D systems) in basal medium RPMI1640 (Cellgro ...
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bioRxiv - Systems Biology 2021Quote: ... HGF 50 ng/ml; NT-3 10 ng/ml; PDGF-BB 10 ng/ml; GDNF 10 ng/ml; All from R&D systems). Ligand treatment was started at the time of tetracycline induction ...
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bioRxiv - Cell Biology 2020Quote: ... serum starved at 37°C in MV2 medium (containing no growth factors) 3 hrs before stimulation with VEGFA164 (50 ng/ml; R&D Systems), followed by fixation in 3% PFA for 3 min ...
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bioRxiv - Developmental Biology 2019Quote: ... in Medium B with the addition of 5 U/mL erythropoietin (EPO; Amgen) and 5 ng/mL interleukin-3 (IL3; R&D Systems). Two days later ...
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bioRxiv - Biochemistry 2020Quote: ... and furin (3 nM) in the presence of the fluorogenic substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog no. ES010, R&D systems). The final substrate concentration was 10 μM in 50 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... and further differentiated into day 3 posterior foregut through the addition of CDM3 base media supplemented with FGF2 (20 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... BMP4 + Activin A treatment was accomplished by treating cells with mTeSR supplemented with 3 ng/mL recombinant human BMP4 protein (R&D Systems) and 100 ng/mL recombinant human Activin A protein (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in low cluster plates to allow EB formation in serum-free differentiation SFD media supplemented with BMP4 (3 ng/ml, R&D Systems). At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... The murine MLL-AF9 AML cells (30) were cultured in the same condition with 10 ng/mL rmIL-3 (R&D systems). Culturing of normal murine HSPCs and human CD34+ UCBs cells has been described previously (31).
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
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bioRxiv - Molecular Biology 2022Quote: ... iPSC at 60-70% confluence were treated for 3 days with 100 ng/mL Activin A and 100 ng/mL bFGF (R&D Systems) in the presence of increasing levels of FBS (0% on day 1 ...
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bioRxiv - Immunology 2023Quote: ... purified hCD8+ T cells were subjected to 3 consequent cycles of αCD/αCD28 activation in the presence of 50 ng/mL TGFβ (R&D Systems) and 30U/mL rhIL-2 lasting 24h each.
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bioRxiv - Immunology 2023Quote: ... centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat ...
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bioRxiv - Cell Biology 2023Quote: Primary human lung fibroblasts (passages 3-9) were exposed to 5ng/ml recombinant human TGF-β1 (R&D Systems, 240-B-002), 180μM H2O2 (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Resting or ADP (10 μM)-stimulated platelets (4 × 103/μL) were pretreated with the inhibitor hDcR3 (10 μg/mL, recombinant human decoy receptor 3 protein, R&D Systems) for 10 min and then allowed to adhere to the coated coverslips for 60 min at RT under normoxic or hypoxic conditions ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels (n=3) were incubated for 4 hours in a solution of FBS-free DMEM supplemented with 10% Resazurin dye (AR002, R&D Systems) at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 5 ng mL-1 of IL-7 and IL-15 (R&D Systems). Regulatory T cells were activated at 1 x 106 cells mL-1 for 2 days in complete XVivo15 supplemented with magnetic Treg Xpander CTS Dynabeads (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... and lipocalin-2 (Lcn-2) protein was assayed in Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D Systems, Minneapolis, MN, USA). At the end of DSS treatment ...
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bioRxiv - Cell Biology 2020Quote: We used the Human IL-6 Quantiglo ELISA (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions to determine the concentration of IL-6 protein released from HPAEC cultured under various conditions.
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bioRxiv - Neuroscience 2021Quote: ... or t-PA + plasminogen (R&D Systems, UK; 6, 12 or 120 nM) for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... IL-6 and IL-10 in media (DuoSet, R&D Systems Minneapolis, USA). ELISA procedures were carried out according to manufacturer protocols.
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bioRxiv - Immunology 2020Quote: ... IL-6 and IFN-β (DuoSet mouse ELISA kits from R&D Systems) were performed according to manufacturer’s instruction.
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bioRxiv - Physiology 2021Quote: ... RPMI with B27 minus insulin containing 6 µM CHIR99021 (4423, R&D Systems) was added to the cultures at time zero ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were analysed for cytokine release using IL-6 ELISA (R&D systems) for CpG-DNA and LPS or IFN-β ELISA (R&D systems ...
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bioRxiv - Genomics 2022Quote: ... and murine IL-6 10 ng/mL (R&D Systems #406-ML-025), in a 37°C and 5% CO2 atmospheric environment.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the IL-6 ELISA kit (R&D Systems Inc., Minneapolis, MN, USA), following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... and the mouse chemoattractant macrophage-inflammatory protein-2 (MIP-2, R&D Systems). Migration of cells was evaluated by trypan blue exclusion following incubation.
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bioRxiv - Cancer Biology 2022Quote: ... 2 μg/mL Fibulin-2 (Cat no. 9559-FB-050, R&D Systems) coated ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell lines were validated for pluripotency markers by immunhohistochemical stainings using the Human Pluripotent Stem Cell 3-Color Immunohistochemistry Kit (R&D Systems, SC021) and were differentiated into the three different germ layers using the Human Pluripotent Stem Cell Functional Identification kit (R&D Systems ...
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bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 3 independent vials of iCell microglia were seeded at 5 x 104 cells per well across 3 independent 96-well plates and treated the following day with vehicle or 5 ng/mL human IFN-β (R&D Systems). Cells were treated for 6 or 24 h prior to collection and stored at −80°C prior to RNA extraction.
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bioRxiv - Neuroscience 2019Quote: ... Human IFNγ and IL10 were quantified in culture supernatants (1:2 dilution) using DuoSet ELISA kits (R&D Systems). DuoSet ELISA Ancillary Reagent Kit 2 was used in conjunction with all DuoSet ELISA kits ...
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bioRxiv - Cell Biology 2020Quote: ... Autophagy was induced by treating the cells for 2 hours in 250nM Torin-1 (R&D Systems, 4247/10) in 1X Hanks’ Balanced salt solution ...
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bioRxiv - Cancer Biology 2023Quote: ... in 2% BSA/PBS solution and the appropriate fluorescently-conjugated secondary antibody (R&D Systems catalog #NL006, 1:200). Coverslips were mounted onto slide with DAPI-containing ProLong Gold media ...
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bioRxiv - Cell Biology 2020Quote: ... SCs were sorted based on positive alpha 7-integrin (1:100, R&D Systems FAB3518P) and absence of Sca1 (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were kept in enriched N-2 medium with 10 ng/ml of basic fibroblast growth factor (R&D systems, Minneapolis, MN, USA) added every 24 h and medium changed every alternate day to keep the cells in an undifferentiated and proliferative state ...
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bioRxiv - Immunology 2022Quote: ... human IL-2 ELISA (human IL-2 Quantikine ELISA kit, R&D systems, USA) and TNFα ELISA (human TNF-alpha quantikine ELISA kit ...
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bioRxiv - Neuroscience 2019Quote: ... Stattic (6-Nitrobenzo[b]thiophene 1,1-dioxide) was supplied by R&D Systems (UK).
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bioRxiv - Physiology 2019Quote: ... The levels were measured using commercially available IL-6 ELISA (R&D Systems, USA) and phospho-Akt (S473 ...
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bioRxiv - Immunology 2019Quote: ... IL-1β and IL-6 were detected by ELISA duo sets (R&D Systems) following the manual ...
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bioRxiv - Bioengineering 2022Quote: ... ELISAs for TNF-α and IL-6 (Duoset ELISA, R&D Systems, Minneapolis, MN) and albumin (Immunology Consultants Laboratory ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-6 (406-ML) and LIF (8878-LF) were purchased from R&D Systems. JAK1/2 inhibitor Ruxolitinib was obtained from (Abcam ...