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Citations for R&D Systems :
251 - 300 of 2731 citations for 7 chloro 5 methylpyrazolo 1 5 A pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Test wells were coated with 5 µg recombinant spike protein (rSP; S1+S2; R&D Systems, Minneapolis, MN)/ml coating buffer (0.05 M sodium bicarbonate ...
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bioRxiv - Cell Biology 2020Quote: Mature adipocytes were cultured in complete medium supplemented with AZA (R&D Systems; 5, 10, 20µM; 2 days) and rhPDGF-AB (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2019Quote: ... These wells were suspended above wells containing 5 to 10 ng/mL recombinant human VEGF (R&D Systems) or full serum medium ...
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bioRxiv - Cell Biology 2019Quote: Co-localization experiments were performed on HUVE cells seeded on fibronectin-coated (5 μg/mL, R&D Systems) No ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured in the presence of IL-2 (5 ng/ml; R&D Systems, Minneapolis, MN, USA), and in the presence or the absence of CH-223191 or FICZ ...
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bioRxiv - Pathology 2023Quote: ... Cells were filtered through 40µm cell strainer with 4mL of RPMI + 5% FBS (R&D Systems, Minneapolis, MN), and the unfiltered tissues were further digested with 4mL of 0.05% Trypsin and incubated at 37°C for 10 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with rabbit active Caspase-3 (5 μg/ml, AF835-SP, R&D systems, Inc., Minneapolis, MN), rabbit cleaved Caspase-9 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... magnetically isolated T cells were resuspended in the complete medium and were seeded in a 10-cm petri dish or T-75 flask at 1–2×106 cells/cm2 and cultured in the presence of 20 ng/mL recombinant mouse interleukin-7 (rmIL-7; 402-ML-020/CF, R&D Systems, Minneapolis, MN) for 24 hr at 37°C in a humidified 5% CO2 atmosphere.
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bioRxiv - Immunology 2021Quote: ... 20ng/ml mouse IL-7 (R&D System 407-ML) and 20ng/ml mouse IL-15 (R&D System 447-ML) ...
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bioRxiv - Immunology 2023Quote: ... IL-7 (10 ng/ml; R&D Systems, 207-IL), and TNFα (4.9 ng/ml ...
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bioRxiv - Microbiology 2024Quote: ... Monocytes were differentiated into hMDMs for at least 7 days in complete medium containing 10 ng.mL-1 of recombinant human macrophage colony-stimulating factor (rhM-CSF; R&D systems).
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bioRxiv - Cell Biology 2020Quote: ... the cells undergoing differentiation were cultured in basal medium supplemented with 5% v/v Osteogenic Supplement (R&D Systems) with the control cells cultured in basal medium ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) containing 5% fetal bovine serum (FBS; R&D Systems), gentamicin (50 μg/mL) ...
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bioRxiv - Cancer Biology 2019Quote: ... MCF10A cells were treated for 3 days with 5 ng/ml of TGF-β1 (R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2020Quote: Samples stored at −20C were analyzed using IL-5 and IL-13 ELISA kits (R&D Systems, Minneapolis, MN) as per the company’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 5 fmol or 10 fmol SpyCEP were incubated with 2 pmol human CXCL8 and CXCL1 respectively (R&D Systems), in a final volume of 100 μl and incubated at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... from Sino Biological (10247-HNAB) or PTN (5 ng/µl equivalent to 327 nM) from R&D Systems (R&D252-PL-050). The final volume within each well was 100 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was also measured using 5 nM of recombinant human 26S proteasome (Cat: E-365; R&D Systems).
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bioRxiv - Immunology 2024Quote: ... Cytokine protein levels were measured by ELISA (IL-17E, IL-5, and IL-13 Duo-Set, R&D Systems). Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad) ...
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bioRxiv - Genetics 2020Quote: ... femurs and tibias were harvested and bone-marrow cells were obtained by flushing bones and differentiated for 7 days in DMEM media supplemented with 50 mg ml-1 recombinant macrophage-colony stimulating factor (M-CSF, R&D systems), 20% heat-inactivated FBS ...
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bioRxiv - Neuroscience 2022Quote: ... sonic hedgehog C25II (days 2-7, 100ng/mL, R&D Systems). After day 13 of differentiation ...
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bioRxiv - Immunology 2020Quote: ... IL-7 and stem cell factor (all from R&D systems) in α-MEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... and IL-7 (50 ng/ml, R&D systems, #407-ML). After 2 to 4 weeks ...
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bioRxiv - Biochemistry 2023Quote: ... His10-FLAG-BRD4 (0.2 μL, 7 μM, R&D Systems, SP600100), ubiquitin (10 μL ...
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bioRxiv - Cell Biology 2020Quote: ... The chamber was prepared by adding 26μl pre-warmed (37°C) CXCL-5 (0, 0.5, 50, 500ng/ml) or CCL2 (0, 0.5, 50, 500ng/ml) (R&D systems) in DMEM to the lower chamber ...
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bioRxiv - Microbiology 2021Quote: Cytokine analyses were performed on the 5 mL of collected BALF via DuoSet ELISA kits (R&D Systems, Minnesota, MN). For total and differential cell counts ...
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were subsequently supplemented every two days with 1.5% CMG and 5 ng/mL of RANKL (R&D Systems) to induce osteoclastogenesis.
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bioRxiv - Bioengineering 2019Quote: ... 0.25 ng/mL BMP4 (PeptroTech 315-27) and 5 ng/mL Activin A (R&D Systems 338-AC-050/CF) were added to the SF differentiation medium ...
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bioRxiv - Immunology 2020Quote: ... two group of 5 mice each were treated with monoclonal anti-mouse IL-25 (mα-IL-25) (R&D Systems) or monoclonal anti-mouse IL-4Rα (mα-IL-4Rα ...
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bioRxiv - Microbiology 2023Quote: Cytokine analyses were performed on the 5 mL of collected BALF via DuoSet ELISA kits (R&D Systems, Minneapolis, MN). For total and differential cell counts ...
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bioRxiv - Physiology 2023Quote: ... intra-assay % coefficient of variation [%CV] 5%-2.5%), alanine transaminase (ALT) (Siemens Healthcare, intra-assay %CV 8.1%-3.6%) and growth differentiation factor 15 (GDF15) (R&D Systems, BioTechne ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FBS and Penicillin-Streptomycin) with or without 5 μg/ml recombinant human MANF protein (R&D systems, 3748-MN). For EdU cell proliferation assay and Ca2+ measurement ...
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bioRxiv - Immunology 2020Quote: ... and then brought to 1-2 million PBMCs/mL in R10 buffer plus 500pg/mL rhIL-7 (R&D Systems, Minneapolis, MN, USA) and rested again for four days ...
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bioRxiv - Cell Biology 2019Quote: ... SKBr3 cells co-transfected with EGFP-α-tubulin and the indicated siRNA or cDNA were grown on collagen-coated glass coverslips for 48 h and observed 10 min after the addition of 5 nM HRGβ1 (R&D Systems) using the 63X objective (plan Apochromat NA 1.4 ...
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bioRxiv - Cell Biology 2019Quote: ... C2C12 and cardiac fibroblasts were serum starved for 24 h before adding medium containing 5 ng/ml recombinant TGFβ1 for 2 days (R&D Systems, Minneapolis ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs were encapsulated (5·106 cells/mL) in situ within hydrogels loaded with 200 pmol/mL VEGF-165 (R&D Systems) or within non-loaded hydrogels (without GF) ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cells were incubated with 5 µL of 25 µg/mL anti-hCD3ε (PE Conjugated Mouse IgG1, R&D System) antibody or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC).
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng/mL FGF1 and 5 μg/mL heparin or 10 ng/mL PDGF-AA (R&D Systems #1055-AA-050) supplemented DMEM for 12 hrs.
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bioRxiv - Developmental Biology 2020Quote: ... Micromass cultures were then maintained at 37°C in a humidified incubator with 5% CO2 for 24hrs with the inhibitors before treatment with 5ng TGFβ1/ml (R&D Systems) for the indicated times.
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bioRxiv - Cancer Biology 2019Quote: ... Active CD73 enzyme (Recombinant Human 5’-Nucleotidase/CD73 Protein, CF) and active CD39 (His-Tag Human) were from R&D Systems, Minneapolis ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with increasing doses of TGFβ2 (vehicle control, 1.25 ng/ml, 2.5 ng/ml, and 5 ng/ml; R&D Systems, Minneapolis, MN) for 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
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bioRxiv - Microbiology 2023Quote: ... 100 µg/ml streptomycin and either 5 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Abingdon, UK) to promote M1-differentiation or 20 ng/ml macrophage colony-stimulating factor (M-CSF ...
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bioRxiv - Cell Biology 2022Quote: ... Experiments were done in 293T cells pretreated with 5 ng/ml recombinant human R-spondin 3 (R&D Systems, Minneapolis, USA). Experiments were performed with biological triplicates ...
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bioRxiv - Cancer Biology 2023Quote: ... and washed three times in ice-cold DPBS buffer supplemented with 0.5% BSA and aliquoted to a density of 5 × 106 cells/ml in Flow Cytometry Staining Buffer (FC001, R&D Systems). Next ...
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bioRxiv - Bioengineering 2024Quote: ... and stained with a 5 µg/mL of donkey anti-goat IgG NorthernLights™ NL637-conjugated antibody (R&D Systems; NL002) for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were then incubated with a 5 µg/mL of human HNF-3 beta/FoxA2 antibody (R&D Systems; AF2400) for 1.5 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 2ng/mL rhIL-7 and 0.4ng/mL rhIL-15 (R&D Systems). T cells were transduced 24 hours post-activation with lentivirus encoding an anti-EGFR CAR with 41bb/CD3ζ ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10 ng/mL recombinant human IL-7 (R&D Systems). For culture ...