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Citations for R&D Systems :
401 - 450 of 2686 citations for 7 BENZYL 1 4 DITHIA 7 AZA SPIRO 4.4 NONANE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... B cells were labelled with 1μM DDAO (Thermo) and cultured in full RPMI supplemented with 4 μg/ml CD40L (R&D systems), 100ng/ml BAFF (Peprotech) ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Pathology 2023Quote: ... Tissue sections were labeled overnight at 4°C in a humid chamber with goat anti-mouse CLEC4F (AF2784, R&D systems) and rat anti-mouse/human CD324 (DECMA-1 ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies (FUS 1:100 Santa Cruz Biotech sc-47711; ISL1 1:500 Abcam ab109517; OLIG2 1:100 R&D Systems AF2418; NFM 1:1000 Merck MAB1621) were added in 1% BSA and incubated at 4degC overnight ...
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bioRxiv - Cell Biology 2020Quote: ... anti-decorin (1:500, 1% casein, AF1060, R&D Systems, MN, USA), anti-biglycan (1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... or 1 ng/mL PTH aa1-34 (R&D systems 3011/1) to αMEM media induced osteogenesis ...
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bioRxiv - Microbiology 2021Quote: ... or anti-hNP-1 (2.5 – 20 μg ml−1; R&D Systems) antibodies before serum-adaptation was carried out.
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bioRxiv - Developmental Biology 2022Quote: ... Prox1 (11-002, AngioBio, 1:200; AF2727, R&D Systems, 1:200), LYVE1 (AF2125 ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti IGFBPL-1 (R&D Systems, product no: AF4130, (1:50)) ...
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bioRxiv - Neuroscience 2022Quote: ... anti-PAX6 (BioLegend, 901301, 1:300; R&D Systems, AF8150, 1:500), anti-PCNA (Santa Cruz Biotechnology ...
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bioRxiv - Physiology 2019Quote: ... including anti-PEACAM-1 antibody (goat, 1:400, R&D Systems, USA), anti-NG2 antibody (rabbit ...
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bioRxiv - Immunology 2023Quote: ... and IL-1 beta/IL-1 F2 DuoSet ELISA (R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... goat anti-jagged 1 (1:150, AF599, R&D Systems, RRID: AB_2128257), rabbit anti-RET (1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... goat anti-jagged 1 (1:150, AF599, R&D Systems, RRID: AB_2128257), rabbit anti-RET (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse VEGFR1/Flt-1 antibody (1 μg/mL, R&D Systems, #AF471), or a combination of anti-VEGFR1 and VEGF-B for 30 hrs ...
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bioRxiv - Cancer Biology 2021Quote: ... MO) or using 50 μg/ml of LPS plus 50 ng/ml of mouse IL-4 from R&D Systems (Minneapolis, MN). After 4 days in complete RPMI media at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... and genomic DNA regions of interest were isolated using 4 μg of antibody against Brachyury (R&D Systems, cat# AF2085, lot# KQP0719121). Complexes were washed ...
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bioRxiv - Bioengineering 2021Quote: ... primary antibodies (E-cadherin, AF748, R&D Systems, 4 μg/mL; vimentin, ab92547, Abcam, 0.5 μg/mL; CD31, AF3628, R&D Systems, 10 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked with blocking buffer for 30 min at room temperature and followed by incubation with primary antibody overnight at 4°C (all primary antibodies used for dot blot were purchased form, R&D systems; anti-Cathepsin A ...
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bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
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bioRxiv - Cancer Biology 2021Quote: Cells were grown to 70% confluent in 24-well plates prior to treatment with N-[2-[[3-(4-Bromophenyl)-2-propenyl]amino]ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89; R&D Systems) or 3-(1,3-Benzodioxol-5-ylmethylene)-2-oxo-1-pyrrolidinecarboxaldehyde (KNK437 ...
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bioRxiv - Immunology 2022Quote: The supernatants collected from LPS-stimulated moDCs with or without fhFABP1 (25 μg/ml) were pooled from 4 donors in equal volume and subjected to the Proteome Profiler Human XL Cytokine Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... supplemented with recombinant human IL-4 (50 ng/mL) and recombinant human GM-CSF (100 ng/mL) (R&D Systems, Minneapolis, MN) with one additional supplement of fresh media and cytokines at day 3 of culture as previously described 12 ...
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bioRxiv - Immunology 2020Quote: ... were generated by incubation of bone marrow cells for 5 days in the presence of 10 ng/ml of recombinant murine GM-CSF and IL-4 (R&D Systems), as described previously [40] ...
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bioRxiv - Immunology 2019Quote: ... Immature dendritic cells were prepared by treatment of monocytes with 500 U/ml human IL-4 and 800 U/ml human GM-CSF (R&D Systems) in CellGenix media for 6 days ...
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bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Cell Biology 2019Quote: ... slides were blocked and stained using rabbit anti-LOXL2 (Gil25-70, Gilead Sciences) antibody overnight at 4° C and a rabbit cell & tissue staining kit (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked in 5% nonfat dry milk in TBST and probed overnight at 4°C with antibodies recognizing ERAP1 (AF2334, R&D Systems), ERAP2 (AF3830 ...
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bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
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bioRxiv - Immunology 2020Quote: ... non-tissue culture plates were coated overnight at 4゜C with recombinant human B7-H3 protein (R&D Systems, Minneapolis, MN) at 10 μg/well in 500 μL of PBS ...
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bioRxiv - Cell Biology 2023Quote: ... 1 x 106 BM cells were incubated at 37°C or 4°C for 45 min with or without Cxcl12 (10 nM, R&D Systems). The reaction was stopped by adding ice-cold RPMI and brief centrifugation at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... separated and blotted onto 0.2 μm nitrocellulose as above and incubated overnight at 4°C in blocking buffer supplemented with 2 μg/ml polyclonal sheep IgG anti-LL-37 (R&D Systems). Membranes were washed in wash buffer (PBS with 0.05% Tween ...
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bioRxiv - Developmental Biology 2023Quote: ... embryonic NSC media was prepared as above without epidermal growth factor and with the addition of 50 ng/mL mouse bone morphogenetic protein 4 (R&D Systems, 5020-BP-010 ...
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bioRxiv - Neuroscience 2023Quote: ... and B27 were omitted and lt-NES cells were cultured at low density in a differentiation-defined medium (DDM) in the presence of bone morphogenetic protein 4 (BMP4) (10 ng/mL, R&D Systems), wingless-type MMTV integration site family ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated overnight at 4°C in blocking buffer with anti-E-cadherin (R&D Systems; Minneapolis, MN, USA; cat:MAB1838), anti-integrin-⍺-6 (Stem Cell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Resting or ADP (10 μM)-stimulated platelets (4 × 103/μL) were pretreated with the inhibitor hDcR3 (10 μg/mL, recombinant human decoy receptor 3 protein, R&D Systems) for 10 min and then allowed to adhere to the coated coverslips for 60 min at RT under normoxic or hypoxic conditions ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels (n=3) were incubated for 4 hours in a solution of FBS-free DMEM supplemented with 10% Resazurin dye (AR002, R&D Systems) at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Adherent cell were then cultured in RPMI supplemented with 10% heat-inactivated human serum and L-Glutamine and gentamycin as above (RPMI10%HuS) and 30 ng/ml GM-CSF (Immunex) and 10 ng/ml IL-4 (R&D Systems) for 5 days50 ...
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bioRxiv - Cancer Biology 2020Quote: ... PDX1 (Cell Signaling; #5679S, western blot dilution 1:1000, IHC dilution 1:100; R&D Systems; AF2517, IF dilution 1:100), NR5A2 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... prior to 1 hour incubation of CD206 (1:200, R&D systems, AF2535), F480 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... and 10 ng/mL neuregulin-1 (recombinant human NRG-1; R&D Systems)) at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-βIII-Tubulin mAB (clone TuJ-1, 1:1000, R&D Systems MAB1195). The membranes were incubated with the primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD31/PECAM-1 (dilution 1:300, goat polyclonal, AF3628 R&D Systems) + anti-goat AF546 (dilution 1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... polyclonal anti-goat TIMP-1 (R&D Systems; Minneapolis, MN; 1:300 dilution), monoclonal anti-mouse microtubule-associated protein 2 (MAP2 ...
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bioRxiv - Cell Biology 2019Quote: ... and Mfge8 (Thermo MA5-23913, 1:50; R&D Systems MAB2805, 1:50). All Ltf and Mfge8 antibodies have been previously validated by staining lactating mammary glands.
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bioRxiv - Cell Biology 2021Quote: ... IGF-1 (mouse/rat IGF-1 ELISA kit, R&D system, Minneapolis, MN) and total thyroxine (total thyroxine ELISA ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies Nkx6.1 (1:100, DSHB) and Pdx1 (1:100, R&D Systems) were added and incubated for 30 minutes ...