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Citations for R&D Systems :
401 - 450 of 2926 citations for 7 Amino 4 benzyl 4H benzo 1 4 oxazin 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Sterile tissue culture plates were coated overnight at 4°C with 5 µg/mL recombinant CD62P protein (R&D Systems, #137-PS-050). The following day the wells were washed 3X with PBS before being blocked with 1% BSA in PBS at room temperature (RT ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of isolated EV sample was mixed with 4 μL of Alexa488 anti-human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN) and 2 μL of PE anti-human CD63 antibody (561925 ...
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bioRxiv - Genomics 2023Quote: ... was applied for 30 minutes at room temperature followed by primary antibody staining overnight at 4°C using anti-CD206 (R&D systems, AF2534), anti-CD11b (Cell Sciences ...
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bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... cells were stained for 40 min at 4°C with fluorophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Cell Biology 2023Quote: ... 2ng/mL rhIL-7 and 0.4ng/mL rhIL-15 (R&D Systems). T cells were transduced 24 hours post-activation with lentivirus encoding an anti-EGFR CAR with 41bb/CD3ζ ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10 ng/mL recombinant human IL-7 (R&D Systems). For culture ...
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bioRxiv - Systems Biology 2023Quote: ... and stained for total and relevant phosphoproteins with the following antibodies in one of the three tubes: Tube 1: mouse monoclonal anti-human RPS6 (Clone 522731, catalog # MAB5436, R&D Systems) and rabbit polyclonal anti-human Phospho-RPS6 (Catalog # AF3918 ...
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bioRxiv - Biochemistry 2022Quote: ... or 1:1000 anti-Human/Mouse/Rat PRL-3 Antibody (R&D Systems, MAB3219, Lot. WXH0419091). Following three washes with 0.1% TBST ...
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bioRxiv - Immunology 2021Quote: ... 115-006-068), 250ng/ml mouse recombinant MEGACD40L (Enzo, ALX-522-120) and 20ng/ml Recombinant mouse IL-4 (R&D system, 404-ML). Cells were collected at specific time points for analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887], α-Snail [R&D Systems AF3639] ...
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bioRxiv - Immunology 2020Quote: Macrophages were centrifuged for 7 minutes at 1200 rpm and then stained for 40 minutes at 4°C with fluor ophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Immunology 2020Quote: ... cells were also incubated overnight at 4°C with a sheep anti-Lamp1 Alexa Fluor 488-conjugated (lysosomal marker) antibody (R&D systems, Cat# IC7985G) at 1:10 or a rabbit anti-CD71 (transferrin receptor ...
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bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
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bioRxiv - Cell Biology 2022Quote: ... MC3T3-E1 Subclone 4 preosteoblasts (ATCC CRL-2593) grown in monolayer were treated with vehicle or CXCL12 (20 μg/ml, R&D Systems, 460-SD) for 4 hours ...
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bioRxiv - Immunology 2022Quote: ... were cultured in OptiMEM reduced serum media buffered with sodium bicarbonate and supplemented with 4% (v/v) fetal bovine serum (FBS, R&D Systems, Minneapolis, MN). The cells were grown at 37 °C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Cell Biology 2023Quote: ... Then lysates were utilized exactly according to the instructions in the Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA Kit (R&D systems, DYC2724-5).
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bioRxiv - Microbiology 2021Quote: ... Serum concentrations of IGF-1 and IGFBP-3 were determined using the Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems) and IGFBP-3 DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Cell Biology 2022Quote: Proteasome activity was assessed similarly by incubating cells with genetic (7 days) or chemical (1 hour) perturbations with Me4BoVS (R&D system; I-190-050). After incubation at 37ºC for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... mouse Integrin alpha 7 Alexa Fluor 750-conjugated Antibody (FAB3518S, R&D Systems), APC anti-mouse CD45 Antibody (103111 ...
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bioRxiv - Bioengineering 2023Quote: ... induction of intermediate mesoderm began using 7 µM CHIR99021 (R&D Systems, 4423), a Wnt agonist ...
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Immunology 2021Quote: ... 109-006-127), 250ng/ml human recombinant MEGACD40L (Enzo, ALX-522-110) and 20ng/ml Recombinant human IL-4 (R&D system, 204-IL-010). Cells were collected at specific time points for analysis.
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bioRxiv - Bioengineering 2022Quote: Transduced/Transfected and sorted hGICs harbouring either Lentivirus-MGT#1 or PiggyBac-MGT#1/4 were seeded as single cells into 6-well plates in RHB-A medium supplemented with 10ng/ml TNFa (R&D Systems, 210-TA-020) or without TNFa as control ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used were rabbit anti-Human Phospho-Smad2/3 (R&D Systems MAB8935, 1:500 dilution), goat anti-histone (Abcam ab12079 ...
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bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used: rabbit anti active caspase 3 (1:200; R&D Systems AF835), and rabbit anti-Ki67 (1:5000 ...
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bioRxiv - Cell Biology 2023Quote: Immunohistochemistry was performed with the following antibodies: Cleaved Caspase 3 (rabbit, R&D Systems, MAB835, 1:100), Hopx (mouse ...
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bioRxiv - Bioengineering 2022Quote: ... α3 (MAB1345; R&D systems), β4 (NBP1-43369) ...
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bioRxiv - Developmental Biology 2022Quote: ... Mesoderm lineage explants were generated using recombinant BMP4/7 heterodimer protein (R&D Systems) at a final concentration of 20g/mL in 1xMMR supplemented with 0.1% BSA ...
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bioRxiv - Immunology 2019Quote: ... 5 ng/ml IL-7 and 5 ng/ml IL-15 (R&D Systems).
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bioRxiv - Immunology 2019Quote: ... media was supplemented with 10 ng/mL recombinant human IL-7 (R&D Systems)(a survival factor) ...
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bioRxiv - Immunology 2023Quote: ... in media containing 10 ng/ml IL-7 (BT-007-010; R&D Systems) at 37°C in 5% CO2 and after 3.5 days ...
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bioRxiv - Immunology 2023Quote: ... 10 ng/ml IL-7 and 5 ng/ml IL-15 (R&D Systems). After 14 days ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... explants were incubated overnight in PBS 3% BSA with primary antibody: anti-Robo1 (R&D Systems, 1:400) or anti-Robo2 (gift from Dr Alain Chédotal 53 ...
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bioRxiv - Developmental Biology 2021Quote: ... in serum-free media (DMEM/F12 supplemented with L-glutamine and penicillin-streptomycin) with added FGF ligands (Fgf7; 4 µM, R&D Systems, Cat. No. 5028-KG-025), the γ-secretase inhibitor DAPT (10 µM ...
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bioRxiv - Immunology 2022Quote: ... immobilized hIgG, or 50 ng/ml recombinant GM-CSF (rGM-CSF) (Bioscience, US) plus 20 ng/ml IL-4 (R&D systems Cat.No. 204-IL-010, US), for 5 days to obtain immature dendritic cells (iDC) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the DE monolayer was incubated for 4 days with the hindgut induction medium supplemented with 500 ng/mL of FGF4 (R&D Systems, cat # 235-F4-025/CF) and 3μM of CHIR99021 (STEMCELL Technologies ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... caspase-3 (R&D Systems, AF835), Endomucin (Santa Cruz ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 ng/mL recombinant human IL-7 (R&D Systems; Bio-techne, Inc.) until use.
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bioRxiv - Cell Biology 2023Quote: ... positive selection marker: Mouse anti-integrin alpha 7 Alexa fluor 647 (FAB3518R, R&D Systems). Cells were rinsed ...
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bioRxiv - Neuroscience 2023Quote: HiPSCs-derived microglia at day 7 of differentiation were stimulated with IFNγ (R&D Systems) 100 ng/ml for 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... and differentiating spermatogonia marker c-KIT (day 7; 0.6 µg/ml, R&D Systems AF2944) overnight at 4 °C ...
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bioRxiv - Bioengineering 2019Quote: Stage 1: 3 days in S1 basal media with 100 ng/ml Activin A (R&D Systems; 338-AC) and 3 μM CHIR99021 (Stemgent ...
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bioRxiv - Genomics 2020Quote: ... one sample was stained with mouse IgG isotype control (clone 11711, R&D Systems) diluted 1:5 in FACS buffer ...
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bioRxiv - Systems Biology 2021Quote: ... 10 ng/ml transforming growth factor beta one (TGFβ1, recombinant human, R&D systems) 25 μg/mL l-ascorbic acid 2-phosphate (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (NBQX; 10 μM; R&D Systems) was then added to determine the effect of AMPA receptor blockade on ePSCs ...
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bioRxiv - Microbiology 2022Quote: ... 100 uM of fluorogenic tripeptide substrate Methionine-Glycine-Proline-7-amido-methylcoumarin (R&D Systems ES017); and 2 ug/mL of MetAP enzyme (R&D Systems 3537-ZN) ...
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bioRxiv - Immunology 2022Quote: ... USA) and cultured for 7 days with 20 ng/ml recombinant M-CSF (R&D Systems) to allow them to differentiate to macrophages ...