Labshake search
Citations for R&D Systems :
151 - 200 of 3552 citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Blots were incubated for 1 h with HRP-conjugated secondary antibodies diluted in 5% milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
-
bioRxiv - Cancer Biology 2022Quote: ... as previously described.30 The concentrations of function blocking antibodies used were: 1 μg mL−1 Anti-laminin 5 antibody (R&D systems, #MAB2144), 1 μg mL−1 α3 anti-integrin antibody ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by incubation with the appropriate primary antibodies in blocking solution at 4°C overnight (1:1000 rabbit anti-IBA1 (FujiFilm Wako Chemicals, Cat # 019-19741) & 1:500 sheep anti-TREM2 (R&D Systems, Cat # AF1729)) ...
-
bioRxiv - Immunology 2021Quote: ... anti-IL-4 (1 μg/ml) R&D Systems Cat# MAB204) and anti-IFN-γ (1 μg/ml R&D Systems Cat#MAB-285). Differentiation of Th17 cells was confirmed by measuring IL-17 expression by quantitative real-time PCR ...
-
bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
-
bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
-
bioRxiv - Immunology 2022Quote: ... PBMCs from healthy individuals were isolated and expanded in vitro for 7 days in the presence of 50 ng/ml of OKT-3 (Miltenyi) and 600 IU/ml IL-2 (R&D Systems) in AIM-V (Gibco ...
-
bioRxiv - Immunology 2023Quote: ... and 50 μg/ml L-Ascorbic Acid)) supplemented with Growth Factor Cocktail (5 ng/ml BMP4, 5 ng/ml bFGF, 10 ng/ml IL-6 (R&D systems, 206-IL), 10 ng/ml Flt3L (PeproTech ...
-
bioRxiv - Molecular Biology 2022Quote: ... dTAG-7 (R&D Systems), at the final concentration of 500 nM ...
-
bioRxiv - Neuroscience 2021Quote: VEGF165b (3 – 30 ng; 5 µl; cat. #3045-VE-025, R&D System, USA), PlGF-2 (3 – 30 ng ...
-
bioRxiv - Immunology 2020Quote: ... and then brought to 1-2 million PBMCs/mL in R10 buffer plus 500pg/mL rhIL-7 (R&D Systems, Minneapolis, MN, USA) and rested again for four days ...
-
bioRxiv - Molecular Biology 2023Quote: ... The concentrations of IL-6 and IL-8 were measured in triplicate with the DuoSet ELISA Development System (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Blots were incubated for 1 hour with HRP-conjugated secondary antibodies diluted in 5% skim milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
-
bioRxiv - Pathology 2022Quote: ... blocking-and antibody-incubations were performed in TBS + 0,1% Tween20 + 5% milk. Following primary antibodies (Suppl. Table 1) were used: anti-NRP1 (1:1000, R&D Systems, Cat. No. AF566), anti-NRP2 (1:1000 ...
-
bioRxiv - Systems Biology 2023Quote: ... and stained for total and relevant phosphoproteins with the following antibodies in one of the three tubes: Tube 1: mouse monoclonal anti-human RPS6 (Clone 522731, catalog # MAB5436, R&D Systems) and rabbit polyclonal anti-human Phospho-RPS6 (Catalog # AF3918 ...
-
bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
-
bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
-
bioRxiv - Developmental Biology 2022Quote: Primary antibodies used were rabbit anti-Human Phospho-Smad2/3 (R&D Systems MAB8935, 1:500 dilution), goat anti-histone (Abcam ab12079 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used: rabbit anti active caspase 3 (1:200; R&D Systems AF835), and rabbit anti-Ki67 (1:5000 ...
-
bioRxiv - Cell Biology 2023Quote: Immunohistochemistry was performed with the following antibodies: Cleaved Caspase 3 (rabbit, R&D Systems, MAB835, 1:100), Hopx (mouse ...
-
bioRxiv - Bioengineering 2022Quote: ... were coated with 4 μg ml-1 recombinant mouse IL-10 receptor alpha (R&D Systems) overnight at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by overnight incubation at 4°C in anti-β3 tubulin (1:100, R&D Systems). Sections were rinsed and incubated with goat-anti-mouse Alexa-488 (1:500 ...
-
bioRxiv - Cell Biology 2023Quote: ... Human VEGFR3/Flt-4 antibody (1:500, AF743) was obtained from R&D systems (Minneapolis, MN). Horse Radish Peroxidase (HRP ...
-
bioRxiv - Developmental Biology 2019Quote: ... in Medium B with the addition of 5 U/mL erythropoietin (EPO; Amgen) and 5 ng/mL interleukin-3 (IL3; R&D Systems). Two days later ...
-
bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity was measured using a chromogenic DEVD cleavage assay according to manufacturer’s instructions (R&D Systems, Minneapolis, MN, #K106-100).
-
bioRxiv - Immunology 2022Quote: ... at a final concentration of 60 pmol/peptide/mL in CTLm supplemented with 400 U/mL IL-4 and 10 ng/mL IL-7 (R&D Systems) for three days ...
-
bioRxiv - Microbiology 2020Quote: Secretory leukocyte peptidase inhibitor (SLPI) and Boc-Gln-Ala-Arg-7-Amino-4-methylcoumarin (BOC-QAR-AMC) were obtained from R&D systems. Camostat mesylate was obtained from Sigma Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... PlGF-2 (3 – 30 ng; 5 µl; cat.465-PL/CF, R&D System, USA), VEGF-E (3 – 30 ng ...
-
bioRxiv - Cell Biology 2022Quote: ... 5 μg/ml heparin and 50 ng/ml BMP-4 (#5020BP, R&D System). Seventy-two hours after induction ...
-
bioRxiv - Immunology 2022Quote: ... IL-4 (50 ng/ml) and TGF-β (5 ng/ml) (R&D Systems) for TH9 cells ...
-
bioRxiv - Microbiology 2024Quote: Interleukin-8 (IL-8) concentrations were determined with the IL-8/CXCL8 DuoSet ELISA kit (R&D Systems, Cat# DY208), CXCL12 with the CXCL12/SDF-1 DuoSet ELISA kit (R&D Systems ...
-
bioRxiv - Neuroscience 2023Quote: ... explants were incubated overnight in PBS 3% BSA with primary antibody: anti-Robo1 (R&D Systems, 1:400) or anti-Robo2 (gift from Dr Alain Chédotal 53 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA) or human IgG isotype control (#31154 ...
-
bioRxiv - Cancer Biology 2022Quote: ... incubated overnight at 4°C in the following primary antibodies: T-LCK (1:1000 R&D Systems), T-LCK (1:1000 Proteintech) ...
-
bioRxiv - Neuroscience 2023Quote: ... The cells were labeled with anti-human SSEA-4 APC-conjugated antibody (1:100, R&D Systems) at 37°C for 30 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissues were stained overnight at 4°C with mouse CD45 antibody (1:200; Af114, R&D Systems). The next day ...
-
bioRxiv - Microbiology 2021Quote: ... and the CD4+-enriched T cell population was cultured in AIM-V medium complemented with 40 U/mL IL-2 (Chiron) and 5 ng/mL IL-7 (R&D systems). Blood samples from HIV-1-infected patients were obtained in the context of the Biomedical Research Program TRANSBioHIV promoted by the French National Agency for AIDS and Hepatitis (ANRS ...
-
bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
-
bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
-
bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
-
bioRxiv - Cell Biology 2020Quote: mOSE cells (1×10^6 cells) were plated 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). RNA was collected 7 days post-TGFB1 treatment (RNAeasy Kit ...
-
bioRxiv - Microbiology 2019Quote: The human IFN-β ELISA Kit (cat. #41410-1) and IL-6 ELISA kit (cat. #D6050) purchased from R&D systems were used to measure the Chlamydia-induced IFN-β and IL-6 respectively ...
-
bioRxiv - Cell Biology 2022Quote: Proteasome activity was assessed similarly by incubating cells with genetic (7 days) or chemical (1 hour) perturbations with Me4BoVS (R&D system; I-190-050). After incubation at 37ºC for 2 hours ...
-
bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
-
bioRxiv - Immunology 2020Quote: ... ELISA for IL-7 was performed using Mouse IL-7 Quantikine ELISA Kit M700 (R&D Systems) according to manufacturer’s instructions.
-
bioRxiv - Bioengineering 2019Quote: Stage 1: 3 days in S1 basal media with 100 ng/ml Activin A (R&D Systems; 338-AC) and 3 μM CHIR99021 (Stemgent ...
-
bioRxiv - Developmental Biology 2023Quote: ... gfCDM media was supplemented with 5 nM BMP-4 (R&D Systems, #314-BP-050), followed by 1/2 media change on d9 and d12 ...
-
bioRxiv - Cell Biology 2020Quote: ... mOSE cells (1×10^6) were first plated as monolayer cultures 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). Four days after the addition of TGFB1 ...
-
bioRxiv - Immunology 2021Quote: ... Mouse IL-1β and TNFα and IL-6 in cell culture supernatants or serum were measured using Human IL-1 beta/IL-1F2 DuoSet ELISA (DY201, R&D Systems), Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401 ...