Labshake search
Citations for R&D Systems :
251 - 296 of 296 citations for 6 Methylheptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by ELISA using the DuoSet ELISA Kits (DY210-05, DY201-05, and DY206-05) from R&D Systems per the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... or 100 ng/ml N-palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cysteinyl-[S]-seryl-[S]-lysyl-[S]-lysyl-[S]-lysyl-[S]-lysine (Pam3CSK4) for 6 h and IL6 secretion measured by ELISA (R&D Systems).
-
bioRxiv - Immunology 2023Quote: ... 3000 – 5000 ASCs per well were cultured in sterile 96-well tissue culture plates (Falcon) in the presence of 20 ng/mL IL-6 (R&D Systems), 100 ng/mL APRIL (R&D Systems ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were stained in 0.5% fatty acid-free albumin in DPBS using 1:20 mouse IgG3 SSEA4-488 clone MC-813-70 (R&D Systems, FAB1435F, lot YKM0409121) and 1:20 mouse IgM TRA-1-60-488 clone TRA-1-60 (BD Biosciences ...
-
bioRxiv - Neuroscience 2023Quote: ... After transfection neurons were washed twice with NBP and treated with 20 μM (2R)-amino-5-phosphonovaleric acid (AP5; R&D Systems, 0105/10), an NMDA receptor antagonist to prevent transfection-induced neuronal overexcitation ...
-
bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested using the Quantikine human IL-6 (sensitivity < 5 pg/mL) ELISA kit (R&D Systems, Inc., Minneapolis, MN) according to manufacturer’s recommended conditions ...
-
bioRxiv - Cancer Biology 2020Quote: IL6 and soluble IL6RA concentrations were assessed in 100 µl cultured media obtained from HMECs or MCF 10A cells propagated under anchorage dependent or anchorage independent conditions with the Human IL-6 DuoSet or Human sIL-6R alpha DuoSet ELISA kit (R&D Systems, Germany) following the manufacturer’s recommendations.
-
bioRxiv - Cell Biology 2020Quote: ... papillary or reticular cell sheets were seeded with HUVECs at 6 000 cells /cm2 in ECGM2ΔFGFΔVEGF supplemented or not with VEGF (R&D systems, Minneapolis MN, USA) at 5 ng/mL ...
-
bioRxiv - Microbiology 2021Quote: The secreted TNF-α and IL-6 cytokines in the culture supernatants were quantified by ELISA using Duo Set ELISA (R&D systems) as per the manufactures instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... A 1:6 series of free-floating tissue was stained for TH (MAB318, MilliporeSigma, Burlington, MA) Nurr1 (AF2156, R&D Systems, Minneapolis, MN) or GFP (AB290 ...
-
bioRxiv - Cancer Biology 2020Quote: ... condition media was collected and estimated quantity of the secretary IL-6 using commercially available ELISA kit (R&D Systems, DY 206-05) with human IL6 antibody (R&D Systems ...
-
bioRxiv - Molecular Biology 2023Quote: ... The concentrations of IL-6 and IL-8 were measured in triplicate with the DuoSet ELISA Development System (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2, Biolegend, San Diego, CA, USA / R&D Systems, Minneapolis, MN, USA), and forkhead box protein A2 (FOXA2 ...
-
bioRxiv - Neuroscience 2023Quote: The IL-6 levels in astrocytes conditioned medium (ACM) were determined using the IL-6 ELISA kit per the manufacturer’s instruction (R&D Systems, Minneapolis, MN). Briefly ...
-
bioRxiv - Immunology 2023Quote: ... and hESCs were seeded at 3×105 cells/well onto 6-well plates and rested for 24hrs prior to stimulation with PBS or rhIL-23 (1290-IL-010, R&D Systems, USA) at concentrations of 1 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Serum levels of cytokines (IL-6, TNF-α, MCP-1 and IL-10) were measured by corresponding ELISA kits (R&D Systems, USA) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: Mouse CXCL1/KC and interleukin-6 concentrations in lung homogenates from infected mice were determined using DuoSet® ELISA kits (R&D Systems, UK) according to manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2021Quote: ... offspring at postnatal day 3 to 6 received 7 intraperitoneal injections with either PBS (controls) or 75 ng of carrier free recombinant mouse interleukin-6 (rmIL6, R&D Systems, Minneapolis MN).
-
bioRxiv - Cell Biology 2020Quote: ... IL-6 and TNFα cytokine levels in the media were then measured with DuoSet ELISA kits (R&D systems, catalog number DY406 and DY410) according to manufacturer’s protocol.
-
bioRxiv - Immunology 2021Quote: ... IL-6 was detected in culture supernatants from MSC/T cell co-culture assays by magnetic Luminex assays (R&D Systems, Biotechne, Abingdon, UK) as previously described [31] ...
-
bioRxiv - Neuroscience 2019Quote: ... IL-6 concentrations in maternal blood plasma (3 hours post-injection) were determined using a rat-specific ELISA DuoSet (R&D Systems, Abingdon, UK) as reported elsewhere [27] ...
-
bioRxiv - Microbiology 2019Quote: ... Supernatants were harvested at the 24h post-treatment time point and analyzed for cytokine content using ELISA for IL-6 (R&D Systems; Minneapolis, MN) according to the manufacturer’s protocol
-
bioRxiv - Physiology 2021Quote: ... primary osteoclast precursors were seeded after initial culture on non-treated plates into 6 well plates in the presence of 30ng/mL recombinant mouse M-CSF (R&D systems, Minneapolis, MN) for 3 days ...
-
bioRxiv - Immunology 2022Quote: ... IL-6 cat # D6050, IL-1Ra cat # DRA00B, IFN-γ cat #DY285B, IL-10 cat #DY21YB, IL-17 cat# DY317, R&D Systems, USA), following the instructions of the manufacturer ...
-
bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
-
bioRxiv - Immunology 2023Quote: Bronchoalveolar lavage fluid or conditioned culture media were collected for measurement of IL-6 with a Duoset ELISA kit (R&D Systems, DY406-05). Capture antibody was coated overnight in a 96 well plate at room temperature ...
-
bioRxiv - Bioengineering 2020Quote: ... The mice remained on Western diet for two more weeks before euthanasia and evaluating serum concentrations of TNF-α and IL-6 via enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis, MN).
-
bioRxiv - Biochemistry 2022Quote: ... mice aged 6.5 weeks were starved for 6 hours and intraperitoneally injected with 220ng/g body weight of recombinant mouse FGF23 (2629-FG-025 R&D Systems, Minneapolis, MN, USA) in PBS-BSA 0.1% or PBS-BSA 0.1% vehicle with injection volume of 4μL/g body weight ...
-
bioRxiv - Bioengineering 2022Quote: Transduced/Transfected and sorted hGICs harbouring either Lentivirus-MGT#1 or PiggyBac-MGT#1/4 were seeded as single cells into 6-well plates in RHB-A medium supplemented with 10ng/ml TNFa (R&D Systems, 210-TA-020) or without TNFa as control ...
-
bioRxiv - Neuroscience 2022Quote: ... a complete media change was performed with CDMIII containing respective compounds at a final concentration of 8.8 ng/ml (422.6 nM) IL-6 (R&D Systems, Cat. no. 7270-IL-025) and 25 ng/ml (422.6 nM ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in 6 well plates and then mock-treated or treated with 5000 U/mL of human recombinant IFNβ (R&D systems, 8499-IF/CF) for 24 hours.
-
bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
-
bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... the cells were plated at 0.2M cells/ml in a 6-well plate and stimulated with IFNβ (500 IU/ml, R&D systems cat. 8499-IF-010). At 24h ...
-
bioRxiv - Developmental Biology 2023Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...
-
bioRxiv - Physiology 2021Quote: The concentration of pro-collagen 1α1 concentration in the peritendinous space was determined at select time points after amino acid consumption using a DuoSet® ELISA from R&D Systems (DY6220-05, Minneapolis, MN). Due to the large dialysate volume needed for the pro-collagen assay ...
-
bioRxiv - Neuroscience 2024Quote: ... is sf21 (baculoviris)-derived mouse CX3CL1/Fractalkine protein of 34 kDa and 312 amino acids and recombinant soluble Fractalkine (R&D systems, cat. #571-MF, Accession# AAB71763) is E ...
-
bioRxiv - Cell Biology 2020Quote: Supernatant of confluent pulmonary endothelial cells were analyzed for TNF-α and IL-6 by using a mouse-specific ELISA from R&D Systems (Minneapolis, MN, USA) according to manufacturer’s protocol with the help of an Infinite M200 PRO plate reader (TECAN Instruments ...
-
bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...
-
bioRxiv - Molecular Biology 2023Quote: ... The sections were blocked with 5% skimmed milk in TBS-T at room temperature for 60 min and then incubated with rabbit anti-mMCP-6 antibody (R&D Systems, MN, USA, 1:500) at 4 °C overnight ...
-
bioRxiv - Bioengineering 2023Quote: The conditioned media from treated RAW264.7s was used to quantify levels of multiple secreted cytokines/chemokines using their respective ELISA kits including IL-6 (DY406; R&D Systems Incorporated, Minneapolis, MN, USA), TNF-⍰ (DY410 ...
-
bioRxiv - Cell Biology 2024Quote: ... TNF-α and IL-6 release was measured using DuoSet ELISA kits according to the manufacturer’s instructions (R&D Systems: DY417-05; DY410-05; DY406-05).
-
bioRxiv - Physiology 2019Quote: ... and hyaluronan were measured using commercial enzyme-linked immunosorbent assay kits (IL-6: BioLegend, San Diego, CA, USA; corticosterone: Enzo Life Science Inc., Farmingdale, NY, USA; hyaluronan: R&D Systems, Minneapolis, MN, USA), according to the manufacturers’ instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... TNF-α (MTA00B) and IL-6 (M6000B) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits (ELISA Development System; R&D Systems, McKinley Place, MN, USA) following the manufacturer’s protocol.75
-
bioRxiv - Developmental Biology 2024Quote: ... containing 40 mM DTT (ITW Reagents, Catalog No. A2948) or cultured in 2D on plates coated with 6 μg/ml laminin (R&D Systems, Catalog No. 3446-005-01). Cultures were done in TEC medium ...
-
bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...