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Citations for R&D Systems :
301 - 350 of 3663 citations for 6 Methyl 1 2 4 triazolo 4 3 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 100 ng/ml Act A + 50 ng/ml bone morphogenetic protein-4 (BMP4, R&D Systems), (iv ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated with 4 µg of purified GST-tagged ISG15 (R&D systems, UL-600-500) or GSTP1 (#G5663 ...
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bioRxiv - Cancer Biology 2022Quote: ... and then incubated overnight at 4°C with goat polyclonal antibody to ST6GAL1 (R&D Systems) (see Table S3 for antibody information) ...
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bioRxiv - Molecular Biology 2022Quote: ... M2 macrophages were generated by stimulating Mϕ with 20 ng/ml IL-4 (R&D Systems) and 20 ng/ml IL-13 (R&D system ...
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bioRxiv - Neuroscience 2019Quote: ... samples were incubated overnight at 4°C with anti-TRKB (#AF1494, R&D systems, United Kingdom), anti-CB1 (#CB1-Rb-Af380 ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested after 4 h and assayed for IFN-γ via ELISA (R&D Systems).
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bioRxiv - Physiology 2023Quote: ... Sections were then stained overnight at 4°C using primary antibody for SOX9 (R&D Systems) or phosphor-EGFR (phosphoTyr-1173 ...
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bioRxiv - Immunology 2023Quote: ... and stimulated with 0.3 μg/ml HEL plus 20 ng/ml IL-4 (R&D Systems), 0.2 μg/ml CpG (InvivoGen ...
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bioRxiv - Genomics 2023Quote: ... membranes were probed overnight at 4°C with antibodies that recognize ERAP2 (AF3830, R&D Systems) or α-tubulin (T9026 ...
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bioRxiv - Physiology 2024Quote: ... then stimulated with recombinant human IL-4 at 50 ng/mL (R&D Systems, Minneapolis, MN) in BEBM medium for 6 h or BEBM medium as control condition ...
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bioRxiv - Cancer Biology 2021Quote: SUM159-LM1 cells or MDA231-LM2 cells were suspended in HuMEC/0.1% BSA and treated with EC-derived conditioned media (CM) (diluted 2-5 times in the final medium) or 50 ng/ml of recombinant activin B (R&D systems). 2,500 cells/well of SUM159-LM1 cells or 5,000 cells/well of MDA231-LM2 cells were seeded on 96-well ultra-low attachment plate (Corning) ...
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bioRxiv - Immunology 2022Quote: ... The single memory B cells were cultured in complete RPMI 1640 medium supplemented with IL-2 (10 U/ml, R&D Systems), IL-21 (10 U/ml ...
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bioRxiv - Cell Biology 2021Quote: ... cleaved Caspase 3 (rabbit, R&D systems MAB835, 1:500), CPE (rabbit ...
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bioRxiv - Microbiology 2022Quote: ... and anti-Tyro-3 (1:1000, R&D Systems, MAB859100) antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3× in 1× wash buffer (895003, R&D Systems), blocked in 1% BSA for 1 hour at room temperature (RT) ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and then treated with 10 ng/mL of recombinant human TGF-b (R&D Systems; 240-B-002) or 10 ng/mL of recombinant human IL-1β (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in B cell Excellerate medium with the CEllXVivo B cell expansion kit (R&D systems, UK). CD8+ T-cells (Cambridge Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... After 4 days cells were re-stimulated and cytokine production was measured by ELISA (R&D Systems).
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bioRxiv - Neuroscience 2020Quote: ... 4) incubation with a donkey anti-rabbit IgG conjugated with NorthernLights™ NL557 (#NL004, R&D System) diluted 1:1000 for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and stained overnight at 4°C with goat anti-human SPP1 antibody (R&D Systems, AF1433-SP), followed by secondary staining with donkey anti-goat AF594 (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant human IL-4 500 IU/mL and GM- CSF 800 IU/mL (both R&D systems) at a cell density of 1-2 x106 cells/mL for 5 days ...
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bioRxiv - Pathology 2024Quote: ... Cells were then immunolabeled for 4hr at 4°C with sheep anti-CD31 (R&D Systems, AF806) + rabbit anti-cTNT (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... Mg-ATP (R&D systems B-20) and 10x E3 Ligase Reaction Buffer (R&D systems B-71) ...
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bioRxiv - Systems Biology 2022Quote: ... TGFB1 (R&D systems, 240-B-002) was reconstituted at 100ug/mL in a 4mM hydrochloric acid ...
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bioRxiv - Cancer Biology 2023Quote: ... and granzyme B ELISA (R&D Systems) after 48 hours of incubation at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were refreshed in DMEM + 1% (v/v) PS + 1% (v/v) B-27 + 50 ng/ml EGF (Cat. No. 236-EG, R&D System) + 25 ng/ml FGF7 for 4 days ...
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bioRxiv - Cell Biology 2022Quote: ... NED] and 3U.ml−1 EPO [Roche]) supplemented with 1 ng.ml−1 IL→ 3 and 10 ng.ml−1 SCF (both R&D Systems) and 1 µg.ml−1 doxycycline for 4 days ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-Active Caspase-3 (R&D Systems; AF835; 1:250); mouse anti-Mucin 5AC (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... Immunohistochemical assays for Caspase 3 (1:1000, R&D systems, AF835), PDGFR-β (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2021Quote: ... 25 ng mL-1 IL-3 (203-GMP, R&D Systems), 2 mM GlutaMAX ...
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bioRxiv - Cancer Biology 2023Quote: ... and IGFBP-3 (cat. no. AF675; R&D SYSTEMS, 1:1000) were used for Western blot analysis and Immunocytochemistry assays.
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bioRxiv - Developmental Biology 2021Quote: ... The cells were treated for 2 days (days 6, 7) with 50 ng/ml sonic hedgehog (SHH; R&D Systems), 2 μM dorsomorphin (DSM ...
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bioRxiv - Immunology 2021Quote: ... were coated with 0.5 μg/mL rhCTLA-4-Fc chimera (7268-CT, R&D Systems, Minneapolis, MN, USA) diluted in 1× PBS pH 7.0 and incubated at 2-8°C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... hiPSCs were incubated with the primary antibody goat anti-human OCT3/4 (R&D Systems, Minneapolis, MN, USA), mouse anti-human SOX2 (R&D Systems) ...
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bioRxiv - Genomics 2021Quote: ... At d1 mice received an intravitreal injection of 4 µg FN14 decoy receptor (1610-TW, R&D Systems) solved in 1 µl PBS in one eye and the same volume of PBS in the other eye ...
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bioRxiv - Genomics 2021Quote: ... At d1 mice received an intravitreal injection of 4 µg FN14 decoy receptor (1610-TW, R&D Systems) dissolved in 1 µl PBS in one eye and the same volume of PBS in the other eye ...
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bioRxiv - Immunology 2022Quote: ... were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody (R&D Systems, # MAB306). The next day ...
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bioRxiv - Developmental Biology 2019Quote: ... after which were incubated overnight at 4°C with goat antihuman IGF2 antibody (R&D systems AF-292) diluted 1 ...
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bioRxiv - Microbiology 2020Quote: ... Sigma Aldrich)] or M2-activation medium [RPMI160 medium with 20 ng/ml IL-4 (404-ML-010; R&D systems) and 100 ng/ml MCSF (416-ML-010 ...
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bioRxiv - Cell Biology 2020Quote: ... slides were incubated overnight at 4 °C in primary antibody (goat anti-mouse Podocalyxin (AF1556, R&D systems) for EC detection (Li ...
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bioRxiv - Immunology 2022Quote: ... Detection of IL-13 and IL-4 in lung homogenates was determined using Duoset ELISA (R&D system) as per manufacturers instructions.
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bioRxiv - Neuroscience 2022Quote: ... were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL, R&D Systems). The readout was HRP-conjugated anti-His tag antibody recognizing the pentraxin proteins (abcam).
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bioRxiv - Bioengineering 2023Quote: ... medium was changed every day to HDM with 20 ng/mL human recombinant BMP-4 (R&D Systems) and 20 ng/mL human recombinant FGF-4 (R&D Systems) ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated overnight at 4°C with one of the following antibodies: FGE (R&D Systems, MAB2779 ...
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bioRxiv - Bioengineering 2022Quote: ... 2 ng/ mL IGF-1 (R&D Systems), and 10 ng/ mL HGF (R&D Systems) ...
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bioRxiv - Immunology 2022Quote: ... either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems) or 1 μg/ml goat IgG anti-human TIM-3 (R&D systems ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant human TGFβ1 (100-B-001) and Thrombospondin-1 (3074-TH-050) were purchased from R&D Systems. Matrigel-based hydrogel culture system were employed ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 concentrations in maternal blood plasma (3 hours post-injection) were determined using a rat-specific ELISA DuoSet (R&D Systems, Abingdon, UK) as reported elsewhere [27] ...
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bioRxiv - Microbiology 2023Quote: ... granzyme B (R&D Systems, Minneapolis, MN, USA), and perforin (abcam ...