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Citations for R&D Systems :
201 - 250 of 351 citations for 6 HYDROXY 7 METHYLPURINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Intestinal tissues from mice treated with PBS or spore for 7 d and 45 d were lysed with cell lysis buffer (R&D Systems) supplemented with 1% 0.2 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Genetics 2020Quote: ... femurs and tibias were harvested and bone-marrow cells were obtained by flushing bones and differentiated for 7 days in DMEM media supplemented with 50 mg ml-1 recombinant macrophage-colony stimulating factor (M-CSF, R&D systems), 20% heat-inactivated FBS ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Microbiology 2020Quote: Secretory leukocyte peptidase inhibitor (SLPI) and Boc-Gln-Ala-Arg-7-Amino-4-methylcoumarin (BOC-QAR-AMC) were obtained from R&D systems. Camostat mesylate was obtained from Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
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bioRxiv - Immunology 2022Quote: ... at 37°C in RPMI medium supplemented with 8% human serum and cultured for 7-9 days in the presence of 50 UI/ml of recombinant IL-2 (R&D System).
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bioRxiv - Immunology 2022Quote: ... Sorted cells were plated at 5000 cells per well in a 96 well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant Biotin-tagged K48-linked poly-ubiquitin chains (2-7) and K48-linked poly-ubiquitin chains (2-16) were purchased from R&D Systems and Enzo Life Science ...
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bioRxiv - Immunology 2023Quote: ... Sorted cells were plated at 5000 cells per well in a 96-well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
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bioRxiv - Molecular Biology 2023Quote: ... over the course of 7 additional days with 25 ng/mL M-CSF and 25 ng/mL RANKL (both from R&D Systems), as previously described [17,30,49] ...
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bioRxiv - Cell Biology 2023Quote: ... after which the organoids were cultured for 13 days in E6 supplemented with 50 ng/mL bone morphogenetic protein-7 (BMP7, R&D systems) and 10 ng/mL human epidermal growth factor (hEGF ...
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bioRxiv - Cell Biology 2020Quote: ... plasma (n=11-47) or BALF (n=6-19) by ELISA (R&D systems, Minneapolis, USA). Active TGFβ1 was quantified by a Mink lung epithelial cell (MLEC ...
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bioRxiv - Physiology 2022Quote: ... IL-6 (DY-406) and Ccl2 (DY-479) according to the manufacturer’s manual (R&D Systems). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Non-pathogenic Th17 cell differentiation was performed with IL-6 (25 ng/mL, R&D Systems) and TGF-β1 (2 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... ELISA kits used were Mouse IL-6 DuoSet ELISA (R&D Systems, Minneapolis, Minnesota, DY406-05) and Mouse CXCL1/KC DuoSet ELISA (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... Rat IL-6 DuoSet ELISA (DY506) and Rat IL-1β DuoSet ELISA (DY501) (R&D Systems), and Human IL-1β DuoSet ELISA (DY201 ...
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bioRxiv - Physiology 2021Quote: ... Commercially available kits were utilized to determine levels of IL-6 and CRP (R&D systems).
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bioRxiv - Immunology 2022Quote: ... α-ULPB2/5/6 (mouse, clone 165903) and α-CCR4-APC (clone 205410) from R&D Systems. α-mouse IgG-APC from Southern Biotech ...
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bioRxiv - Immunology 2019Quote: Pediatric PBMCs were stimulated for 6 h with (1) TNF (20 ng/ml, R&D Systems), (2 ...
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bioRxiv - Immunology 2019Quote: IL-6 concentrations in organ homogenates were analyzed using a DuoSet ELISA kit (R&D Systems). For analysis of gene expression via qRT-PCR ...
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bioRxiv - Immunology 2021Quote: ... for M1-polarization or with 50 ng/ml IL-6 (R&D Systems, Minneapolis, MN, USA) and 20 ng/ml IL-4 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... IL-6 and TNF-α were measured by ELISA kits (R&D Systems, Minneapolis, MN, USA) as instructed and quantified by normalization to protein concentrations
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bioRxiv - Neuroscience 2022Quote: ... IL-6 ELISA was performed according to the manufacturer’s protocol (R&D Systems product number DY206), with slight amendments including ...
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bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity was measured using a chromogenic DEVD cleavage assay according to manufacturer’s instructions (R&D Systems, Minneapolis, MN, #K106-100).
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bioRxiv - Immunology 2020Quote: ... and then brought to 1-2 million PBMCs/mL in R10 buffer plus 500pg/mL rhIL-7 (R&D Systems, Minneapolis, MN, USA) and rested again for four days ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: Quantikine® ELISA Immunoassay kits for human IL-1α and IL-6 (R&D Systems, Abingdon, UK) kits utilizing the sandwich ELISA method were performed to quantitate the protein concentrations closely following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 6.25 ng pMHC and 6.25 ng recombinant mouse PD-L1 with 6-His tag (R&D systems) in 2% BSA were injected into each well in this step ...
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bioRxiv - Microbiology 2021Quote: ... interleukin (IL-) 8 and IL-6 were then measured by ELISA per manufacturer instructions (R&D systems). Cells grown under standard incubator conditions (5% CO2 ...
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bioRxiv - Pathology 2020Quote: Human platelet lysate was immunoprecipitated using IgG or anti-cadherin-6 antibody (R&D Systems, catalog # MAB2715) and Protein A beads (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: ... and passaged every 4-6 day in the presence of 5 μM ROCK inhibitor (R&D Systems) until the 4th passage (P4) ...
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bioRxiv - Immunology 2019Quote: ... as well as ESAT-6 tetramer (as above) presence of 1μg/ml Cyclosporine A (R&D Systems) to prevent tetramer-mediated activation of T cells ...
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bioRxiv - Bioengineering 2020Quote: ... The IL-6 and IL-8 enzyme-linked immunosorbent assay kits were obtained from R&D Systems, USA (#DY206 and #DY208 ...
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bioRxiv - Immunology 2020Quote: ... and the level of mouse IL-6 was determined using a DuoSet ELISA kit (R&D systems) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Specificity of staining was confirmed with the rat IgG isotype control (R&D Systems, #6-001-F). Brightfield images were acquired with a NanoZoomer-2.0 HT C9600 digital scanner (Hamamatsu ...
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bioRxiv - Neuroscience 2022Quote: ... and 25 ng/ml (422.6 nM) Hyper-IL-6 (R&D Systems, Cat. no. 8954-SR-025) at day 45 of differentiation ...
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bioRxiv - Neuroscience 2023Quote: ... coated 6 well plates in oRIM supplemented with 2.6 nM (20 ng/mL) IGF1 (R&D Systems). On day 24 ...
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bioRxiv - Immunology 2023Quote: Collected supernatants were assayed for IL-6 and IFN-β using mouse DuoSet kits (R&D Systems). Samples were diluted in PBS and incubated in a 96-well plate pre-coated with either IL-6 or IFN-β capture antibodies for 2h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... were added to devices with THP-1 M1 macrophages while matrix metalloproteinase 7 (MMP7, clone 111433 at 1 μg/ml) and ANGPT2 (clone 85816 at 1 μg/ml) (all from R&D systems, Minneapolis, MN) neutralizing antibodies were added to devices with THP-1 M2 macrophages ...
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bioRxiv - Microbiology 2021Quote: ... were cultured for 7 days in RPMI supplemented with 5% decomplemented autologous human blood plasma and 10 ng/mL M-CSF (R&D system, #216-MC) to obtain MDMs ...
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bioRxiv - Cell Biology 2022Quote: ... Degradation of Lamin-A/C was inhibited by Caspase-6 Inhibitor Z-VEID-FMK (FMK006 R&D Systems). BMDMs were treated with different concentration of Caspase-6 Inhibitor Z-VEID-FMK for 2 h before activating with LPS ...
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bioRxiv - Immunology 2022Quote: Murine cytokine detection Duoset enzyme-linked immunosorbent assay (ELISA) kits were from R&D Systems (mIL-6, mTNF) or from Biolegends (mIL-10) ...
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bioRxiv - Cell Biology 2021Quote: ... The IL-6 and leptin enzyme-linked immunosorbent assay (ELISA) was purchased from R&D systems (Minneapolis, MN), while the Griess assay for nitric oxide (NO ...
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bioRxiv - Bioengineering 2022Quote: ... IL-6 levels from RAW264.7 conditioned medium was quantified using a DuoSet ELISA kit (R&D systems, DY406) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... were seeded on 6 well plate and cultured with recombinant mouse IFNγ (100 units/mL; R&D Systems) and recombinant human IL-1β (5 units/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 50-100μg of protein lysate was treated for 6-8hrs with chABC (100 μU/mL; R&D systems) before being subjected to traditional western blotting ...
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bioRxiv - Physiology 2021Quote: ... and mouse recombinant proteins of IL-6 and TNFα were brought from R&D system (Minneapolis, MN, USA). Mouse anti-eMyHC was purchased form Developmental Studies Hybridoma (DSHB ...
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bioRxiv - Immunology 2021Quote: IFNβ and IL-6 concentrations in organ homogenates were analyzed using mouse DuoSet ELISA kits (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... while Th17 cell cultures were supplemented with IL-6 (20 ng/mL; R&D Systems 206-IL-010), TGF-β (10 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... cell culture supernatants carefully collected and IL-1β and IL-6 levels measured by ELISA (R&D systems) according to the manufacturer’s specifications.
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bioRxiv - Immunology 2020Quote: ... and cultured for 6 days with recombinant GM-CSF (R&D Systems cat. 215-GM, 50 ng/ml) and IL-4 (R&D Systems cat ...