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Citations for R&D Systems :
401 - 450 of 3426 citations for 6 Chloropyrazolo 1 5 a pyrimidine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... coated 6 well plates in oRIM supplemented with 2.6 nM (20 ng/mL) IGF1 (R&D Systems). On day 24 ...
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bioRxiv - Immunology 2023Quote: Collected supernatants were assayed for IL-6 and IFN-β using mouse DuoSet kits (R&D Systems). Samples were diluted in PBS and incubated in a 96-well plate pre-coated with either IL-6 or IFN-β capture antibodies for 2h at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... and cells were passaged as clumps every 5 days using ReLeSR (StemCell) in the presence of 5 μm ROCK inhibitor (R&D Systems). hiPSCs were characterized by positive IF staining of pluripotent stem cell markers OCT4 ...
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bioRxiv - Developmental Biology 2019Quote: ... in Medium B with the addition of 5 U/mL erythropoietin (EPO; Amgen) and 5 ng/mL interleukin-3 (IL3; R&D Systems). Two days later ...
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bioRxiv - Immunology 2023Quote: ... CXCL9 (5 μg, MAB392), mouse IgG1 isotype control (5 μg, MAB002) and mouse IgG2B isotype control (MAB004) all from R&D Systems, at RT for 2 h ...
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bioRxiv - Immunology 2023Quote: ... cells were initially activated at 5 x 105 cells/mL in B cell activation media: 5 µg/mL soluble CD40L (R&D Systems), 10 µg/mL anti-His tag antibody (clone AD1.1.10 ...
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bioRxiv - Neuroscience 2021Quote: ... coated plates in N4 media (N3 with 0.05uM Retinoic acid, 2ng/ml BDNF (R&D Systems #248-BDB) and 2 ng/ul GDNF (R&D Systems #212-GD)) ...
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bioRxiv - Molecular Biology 2020Quote: Hyaluronic acid of COVID-19 patients’ plasma was measured in 1:5 dilution by the enzyme-linked sandwich assay Hyaluronan DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s descriptions ...
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bioRxiv - Cancer Biology 2023Quote: ... spheres incubated under non adherent conditions for 5 days were challenged with the addition of 500 ng/ml human recombinant Netrin-1 (R&D Systems #6419-N1-025) for a period of 20 minutes before collection ...
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bioRxiv - Cell Biology 2021Quote: ... IL-5 (Peprotech and R&D Systems, Minneapolis, MN), erythropoietin (Janssen-Cilag Ab ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 ng/ml GRO-α (R&D Systems, Germany), 20 ng/ml hyper interleukin-6 (kindly provided by S ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5 U/ml interferon γ (R&D Systems). All experiments testing the effects of genetic perturbations were carried out at the non-permissive temperature for large T function (39°C ...
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bioRxiv - Bioengineering 2022Quote: ... and human fibronectin (5 µg/cm2, R&D Systems) for 1 hour at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... 5 μg/ml recombinant human CD229 (R&D Systems) was immobilized on a black 96-well plate (Greiner Bio-One ...
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bioRxiv - Genomics 2019Quote: ... goat polyclonal anti-NKX2-5 (R&D Systems AF2444), dilution 1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... recombinant human VEGF165 (5 ng/ml, R&D Systems), recombinant human FGF basic146 aa (10 ng/ml ...
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bioRxiv - Bioengineering 2023Quote: ... 5 µM Forskolin (F) (R&D Systems, 1099/50), 5 µM RepSox (R ...
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bioRxiv - Neuroscience 2023Quote: ... goat anti-MMP2 (R&D Systems; 5 μg/ml), rabbit anti-Calretinin (Chemicon ...
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bioRxiv - Bioengineering 2023Quote: ... 5 µM RepSox (R) (R&D Systems, 3742/50) and 3 µM CHIR99021 (C ...
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bioRxiv - Bioengineering 2023Quote: ... 5 ng/mL BMP4 (R&D systems, #314-BP), 1 μM CHIR99021 (Stemgent ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant human VEGF165 (5 ng/ml, R&D Systems), recombinant human FGF10 (25 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 ng/ml Recombinant human BMP4 (R&D Systems), 9 ng/ml Recombinant Human/Mouse/rat Activin A (R&D Systems ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µg/ml human MGL-Fc (R&D System) and 1 µg/ml human Dectin-2-Fc (Sino Biological ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant human VEGF165 (5 ng/ml, R&D Systems), recombinant human FGF10 (25 ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... and 5% Fetal Bovine Serum (R&D Systems S11550). Cells were transfected using Lipofectamine 3000 (Thermo Fisher L3000015 ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse IL-4 (5 ng/μl; R&D Systems) and TGFβ1 (2.5ng/μl ...
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bioRxiv - Bioengineering 2024Quote: ... 5 ng/mL human Bmp4 (R&D Systems, Inc.), 3 μM CHIR99021 ...
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bioRxiv - Biophysics 2022Quote: ... The medium was supplemented with 10 ng/ml recombinant mouse IL-2 (rmIL-2) (402-ML, R&D systems) for long-term culture of mouse ILC2s.
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bioRxiv - Immunology 2022Quote: ... supernatants were collected and IL-2 concentration was determined using the Mouse DuoSet IL-2 ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested cells were washed three times with PBS and resuspended in 2/3 Cultrex Type 2 (R&D Systems) and 1/3 Full Ad-DF+++ medium and plated in drops which were allowed to solidify for 30 minutes at 37°C after which they were overlayed with Full Ad-DF+++ medium ...
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bioRxiv - Immunology 2023Quote: ... Lipocalin-2 was measured using the Mouse Lipocalin-2/NGAL DuoSet ELISA kit from R&D Systems (Cat. DY1857) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... fresh DMEM medium with reduced FBS (2%) was changed and the supernatant was harvested 24 hr later for IGF-1 and SDF-1α ELISA assays (R&D Systems, Minneapolis, MN).
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bioRxiv - Cell Biology 2022Quote: ... Degradation of Lamin-A/C was inhibited by Caspase-6 Inhibitor Z-VEID-FMK (FMK006 R&D Systems). BMDMs were treated with different concentration of Caspase-6 Inhibitor Z-VEID-FMK for 2 h before activating with LPS ...
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bioRxiv - Immunology 2022Quote: Murine cytokine detection Duoset enzyme-linked immunosorbent assay (ELISA) kits were from R&D Systems (mIL-6, mTNF) or from Biolegends (mIL-10) ...
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bioRxiv - Cell Biology 2021Quote: ... The IL-6 and leptin enzyme-linked immunosorbent assay (ELISA) was purchased from R&D systems (Minneapolis, MN), while the Griess assay for nitric oxide (NO ...
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bioRxiv - Bioengineering 2022Quote: ... IL-6 levels from RAW264.7 conditioned medium was quantified using a DuoSet ELISA kit (R&D systems, DY406) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... were seeded on 6 well plate and cultured with recombinant mouse IFNγ (100 units/mL; R&D Systems) and recombinant human IL-1β (5 units/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 50-100μg of protein lysate was treated for 6-8hrs with chABC (100 μU/mL; R&D systems) before being subjected to traditional western blotting ...
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bioRxiv - Physiology 2021Quote: ... and mouse recombinant proteins of IL-6 and TNFα were brought from R&D system (Minneapolis, MN, USA). Mouse anti-eMyHC was purchased form Developmental Studies Hybridoma (DSHB ...
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bioRxiv - Immunology 2021Quote: IFNβ and IL-6 concentrations in organ homogenates were analyzed using mouse DuoSet ELISA kits (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... while Th17 cell cultures were supplemented with IL-6 (20 ng/mL; R&D Systems 206-IL-010), TGF-β (10 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... cell culture supernatants carefully collected and IL-1β and IL-6 levels measured by ELISA (R&D systems) according to the manufacturer’s specifications.
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bioRxiv - Immunology 2020Quote: ... and cultured for 6 days with recombinant GM-CSF (R&D Systems cat. 215-GM, 50 ng/ml) and IL-4 (R&D Systems cat ...
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bioRxiv - Cancer Biology 2023Quote: Antibodies used for FACS: Human/mouse/bovine integrin alpha 6/CD49f PE-conjugated antibody (FAB13501P, R&D Systems); PE/Cyanine 7 anti-mouse CD325 (Ep-CAM ...
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bioRxiv - Developmental Biology 2024Quote: ... 6 and 8 the culture medium was replaced with fresh N2B27 + 50 ng/μL BMP4 (R&D Systems) and analyses performed on day 9.
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bioRxiv - Cancer Biology 2020Quote: ... Lcn-2 levels were estimated in the supernatants using Duoset murine Lcn-2 ELISA kit (R&D Systems, Minneapolis, MN), as described in our previous study [53].
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bioRxiv - Immunology 2022Quote: ... recombinant SARS-CoV-2 Spike RBD and recombinant SARS-CoV-2 Spike RBD B.1.617.2 L452R T478K were purchased from R&D systems. Proteins were reconstituted as per the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Additional reagents that were used are 2N Sulfuric Acid Stop Solution (Catalog no. DY994, R&D Systems, Minneapolis, MN), Triphenylphosphine (TPP ...