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Citations for R&D Systems :
401 - 450 of 675 citations for 5 Pyrimidinecarbonitrile 4 hydrazino 8CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... one multiplex panel was used to measure fatty acid binding protein 4 (FABP4) and resistin (R&D Systems, UK); non-esterified fatty acid (NEFA ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human galectin-4 (GAL4 P56470) and gelsolin (GSN Q3SX14) from bovine plasma were purchased from R&D Systems and Sigma respectively.
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour at 4°C and the supernatant was precipitated with antibodies against BACH1 (AF5776, R&D System), or IgG (normal mouse IgG ...
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bioRxiv - Immunology 2020Quote: ... AA-Mφ were generated by stimulating macrophages overnight with 20 ng/ml IL-4 (R&D Systems, Minneapolis, MN). Reg-Mφ were generated by stimulating macrophages overnight with 100 ng/ml LPS and either 200 nM PGE2 (Cayman Chemical ...
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bioRxiv - Immunology 2021Quote: ... IL-4 in cell culture supernatants or peritoneal washes were determined using DuoSet ELISA Development kits (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... CH12F3-2 cells were incubated in culture medium supplemented with10ng/mL IL-4 (R&D Systems #404-ML-050), 1ng/mL TGFβ (R&D Systems #7666-MB-005 ...
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bioRxiv - Molecular Biology 2023Quote: ... Antigen-retrieved sections were incubated overnight at 4 °C with goat anti-CD45 antibody (R&D Systems, AF114-SP) and rabbit anti-CD68 antibody (Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... and 4 post PR8 infection with commercially available anti-FcγRIII antibody (100 µg/mouse; clone: 275003, R&D systems) and/or anti-FcγRIV antibody (200 µg/mouse ...
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bioRxiv - Immunology 2023Quote: ... were diluted to 4 µg/ml in PBS along with 1 µg/ml VCAM-1-Fc (R&D Systems). To coat ...
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bioRxiv - Neuroscience 2023Quote: ... Specified primary antibodies were incubated over-night at 4°C: goat anti-ICAM1 (1:400, R&D systems AF796), rat anti-cluster differentiation 31 (CD31 ...
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bioRxiv - Immunology 2023Quote: ... a 50:50 mixture of Matrigel Matrix GF and 4 mg/ml Cultrex Rat Collagen I (R&D Systems). For time-lapse live imaging experiments ...
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bioRxiv - Biochemistry 2021Quote: ... Test wells were coated with 5 µg recombinant spike protein (rSP; S1+S2; R&D Systems, Minneapolis, MN)/ml coating buffer (0.05 M sodium bicarbonate ...
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bioRxiv - Cell Biology 2020Quote: Mature adipocytes were cultured in complete medium supplemented with AZA (R&D Systems; 5, 10, 20µM; 2 days) and rhPDGF-AB (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... Naïve CD8+ T cells were cultured in R10 media containing 5 ng/mL IL-7 (R&D systems). C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Microvessels with and without 24 h treatment with 5 ng mL-1 human recombinant TNFα (R&D Systems), were perfused with THP1s under low shear stress (∼0.2 dyne cm-2 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies (Claudin-5, Abcam Ab131259, 1:1000; Collagen IV, Abcam Ab6586, 1:500; Hemoglobin, R&D Systems G-134-C ...
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bioRxiv - Biochemistry 2019Quote: ... These wells were suspended above wells containing 5 to 10 ng/mL recombinant human VEGF (R&D Systems) or full serum medium ...
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bioRxiv - Cell Biology 2019Quote: Co-localization experiments were performed on HUVE cells seeded on fibronectin-coated (5 μg/mL, R&D Systems) No ...
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bioRxiv - Bioengineering 2021Quote: Cells were treated with brefeldin A (10 μg ml-1, R&D Systems, 1231/5 in differentiation media) for 2 h prior to fixation by immersion in paraformaldehyde (4% [v/v] in 1 × PBS ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured in the presence of IL-2 (5 ng/ml; R&D Systems, Minneapolis, MN, USA), and in the presence or the absence of CH-223191 or FICZ ...
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bioRxiv - Pathology 2023Quote: ... Cells were filtered through 40µm cell strainer with 4mL of RPMI + 5% FBS (R&D Systems, Minneapolis, MN), and the unfiltered tissues were further digested with 4mL of 0.05% Trypsin and incubated at 37°C for 10 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with rabbit active Caspase-3 (5 μg/ml, AF835-SP, R&D systems, Inc., Minneapolis, MN), rabbit cleaved Caspase-9 (1:50 ...
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bioRxiv - Immunology 2022Quote: ... PE-conjugated anti-CTLA-4 and APC-conjugated anti-CD279 (PD-1) were purchased from R&D System (Minneapolis, MN). Human cytokines from cell culture supernatants were analyzed using ELISA kits for TNF ...
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bioRxiv - Biophysics 2019Quote: ... Ibidi channels IV0.4 (Clinisciences) were coated overnight at 4°C with 10μg/ml human ICAM-l-Fc (R&D Systems) in Phosphate Buffer Solution (PBS ...
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bioRxiv - Immunology 2022Quote: ... and MΦs with IL-4 (20ng/ml) and IL-13 (20ng/ml; denoted ‘IL4/13’ for brevity; R&D Systems) in MΦ media ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4 and then incubated overnight at 4°C with mouse monoclonal anti-CD154 (1:50, R&D systems, MAB617) and rabbit anti-calbindinD28k (1:500 ...
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bioRxiv - Physiology 2020Quote: ... followed by overnight incubation at 4 °C with primary antibodies against UCP1 (1:1000, R&D systems, Minneapolis, MN, USA), 14-3-3ζ (1:1000 Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were applied overnight at 4 °C: goat anti-OTX2 (1:500, R&D Systems, cat# AF1979), mouse anti-CDX2 (1:200 ...
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bioRxiv - Biophysics 2021Quote: ... was accessed by testing the hydrolysis of 2.5 µM Substrate V (7-methoxycoumarin-4-yl-acetyl-RPPGFSAFK-2,4-dinitrophenyl, R&D Systems), a previously known fluorogenic substrate of IDE ...
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bioRxiv - Cell Biology 2020Quote: ... ELISA kits (CCL17, DY364, MCP1, DCP00, MDC, DMD00, IL-4, D4050, GM-CSF, DY215) were purchased from R&D Systems. PDGF-AA ELISA kits (EHPDGF ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with FGF2 (4 ug/mL; Biochemistry Department, University of Cambridge) and TGF-β1 (1.74 ug/mL; R&D Systems) using Vitronectin XF (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated with the appropriate primary antibody overnight at 4°C: anti-IL1 (goat, 1:1000, R&D Systems), anti-phosphorylated tau (AT8 ...
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bioRxiv - Bioengineering 2023Quote: ... the samples were incubated overnight at 4°C with the primary antibodies for A2B5 (1:50; R&D Systems, MAB1416) and NG2 (1:300 ...
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bioRxiv - Cancer Biology 2023Quote: 10^4 LECs were seeded on 96-well plates pre-coated with 50 μl of Cultrex (3433, R&D Systems) and incubated 24 h in the cell incubator ...
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bioRxiv - Biochemistry 2024Quote: ... carboxylesterase 1 (CES1) and trans-epoxy-succinyl-L-leucylamino(4-guanidino)butane (E-64) were purchased from R&D Systems. The respective genes coding for the human recombinant enzymes Histidine triad nucleotide binding protein 1 (HINT1) ...
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bioRxiv - Cancer Biology 2020Quote: ... CTLA-4 and TNF-α levels were quantified by using the commercially available immunoassay kits from R&D systems (Minneapolis, USA) and following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibody incubation was done overnight at 4 °C with following dilutions: anti-PLOD2 mouse at 1:500-1:1,000 (MAB4445, R&D Systems), anti-FERMT3 mouse at 1:500-1:1,500 (ab173416 ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% paraformaldehyde followed by permeabilization and staining with primary antibodies for LOXL2 (1:500, R&D Systems) and Tetramethylrhodamine (TRITC)-conjugated Phalloidin (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... embryoid bodies were plated on Matrigel (0.17mg/mL in DMEM/F12) in NIM with 10uM BMP-4 (R&D Systems #314BP010). At day 16 ...
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bioRxiv - Developmental Biology 2022Quote: ... Definitive endoderm differentiation commenced 3-4 days post-passage onto fresh MEFs using 25 ng/ ml WNT3a (R&D Systems, UK) and 100 ng/ ml Activin-A (Peprotech ...
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bioRxiv - Pathology 2020Quote: ... 4°C) and plasma was analyzed for TNF-α and IL-10 (porcine specific ELISA kit, R&D Systems, Abingdon, UK). For qPCR ...
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bioRxiv - Immunology 2020Quote: ... 511 or 111 coated filters and stimulated for 4 h with 5nM tumor necrosis factor-α (TNF-α; R&D Systems), then human monocytes or monocyte Hoxb8 cells were added ...
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bioRxiv - Molecular Biology 2021Quote: ... and plated at a concentration of 0.5×106 cells/mL in medium containing 50 ng/mL mouse IL-4 (R&D Systems) and 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the tissue sections were incubated overnight at 4°C with antibodies against myeloperoxidase (1:150 dilution; R&D Systems, AF3667), citrullinated histone H3 antibody (1:100 dilution ...
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bioRxiv - Immunology 2020Quote: ... B cells were labelled with 1μM DDAO (Thermo) and cultured in full RPMI supplemented with 4 μg/ml CD40L (R&D systems), 100ng/ml BAFF (Peprotech) ...
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bioRxiv - Systems Biology 2022Quote: ... membranes were then probed with anti-HSD17β1 polyclonal antibody (Abnova, H00003292-M03A, 1:1000 dilution, overnight 4°C; R&D systems, MAB7178 ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated overnight at 4°C with one of the following antibodies: FGE (R&D Systems, MAB2779, 1:1000), LAMP1 (DSHB ...
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bioRxiv - Pathology 2023Quote: ... Tissue sections were labeled overnight at 4°C in a humid chamber with goat anti-mouse CLEC4F (AF2784, R&D systems) and rat anti-mouse/human CD324 (DECMA-1 ...