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Citations for R&D Systems :
401 - 450 of 1889 citations for MIF Macrophage migration inhibitory factor Mouse His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... with addition of pen/strep and growth factors EGF 20 ng/ml and bFGF 20 ng/ml (R&D Systems). The medium was changed every third day ...
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bioRxiv - Microbiology 2021Quote: ... 10ng/ml interleukin 4 (IL-4) and 10ng/ml transforming growth factor beta 1 (TGFβ1) (R&D systems, Minneapolis, MN), and used between days 7-9 of differentiation.
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bioRxiv - Developmental Biology 2019Quote: ... Culture media for treated samples was supplemented with the following factors as required: 35 ng/ml Bmp4 (R&D Systems), 0.5 μM BIO (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng/ml human transforming growth factor-β3 (TGFβ3; cat. num. 243-B3-010/CF; R&D Systems, Minneapolis, MN), 1 µM Wnt-C59 ...
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant factors (murine FGF7, Peprotech, 450-60; human BMP3, Peprotech, 120-24B; rat CTGF, R&D Systems, 92-37C-T050) were resuspended in 0.1% BSA/PBS and used at final concentration 100 ng/mL.
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bioRxiv - Molecular Biology 2023Quote: The recombinant human transforming growth factor beta (TGFβ) protein and recombinant human TSP2 protein were obtained from R&D Systems. The SMAD3 Inhibitor SIS3 was procured from Cayman Chemical Company ...
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bioRxiv - Cancer Biology 2023Quote: ... The strained cell pellet was resuspended in >80% ice cold Cultrex growth factor reduced BME type 2 (R&D Systems) in PDAC organoid medium ...
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bioRxiv - Cell Biology 2023Quote: ... The concentration of tumor necrosis factor α (TNFα) in supernatants was determined using TNFα ELISA Kit (R&D Systems, USA) and Nunc MaxiSorp ELISA Plates (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... and levels of tumor necrosis factor-α (TNF-α) were assessed by ELISA within 3 days (DY410; R&D Systems). The cytokines TNF-α ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in cold PBS containing 5.6mg/mL Cultrex Reduced Growth Factor BME (R&D Systems, 3533-010-02). Primary tumor and metastatic tumor burdens were measured weekly for 4 and 6 weeks for tail vein injection models and orthotopic models ...
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bioRxiv - Physiology 2023Quote: ... intra-assay % coefficient of variation [%CV] 5%-2.5%), alanine transaminase (ALT) (Siemens Healthcare, intra-assay %CV 8.1%-3.6%) and growth differentiation factor 15 (GDF15) (R&D Systems, BioTechne ...
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bioRxiv - Cancer Biology 2023Quote: ... then mixed with 50 μl Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BME001-05; R&D Systems, Minneapolis, MN) on ice ...
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bioRxiv - Genetics 2023Quote: ... and vascular endothelial growth factor (VEGF) by sandwich ELISA using antibody pairs and standards from R&D Systems (Minneapolis, MN), Invitrogen (Carlsbad ...
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bioRxiv - Biophysics 2020Quote: ... Purified mouse CD45RABC extracellular domain with a C-terminal 6-His tag (accession #: NP_001104786, catalog number: 114-CD-050) was purchased from R&D systems. Lectins Sambucus Nigra Lectin (SNA ...
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bioRxiv - Cancer Biology 2019Quote: ... Active CD73 enzyme (Recombinant Human 5’-Nucleotidase/CD73 Protein, CF) and active CD39 (His-Tag Human) were from R&D Systems, Minneapolis ...
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bioRxiv - Biochemistry 2021Quote: ... TNFα and IL-6 in supernatants were measured using ELISAs (Mouse TNFα; Mouse CCL5; Mouse IL-6, R&D Systems) according to the manufacturer’s protocols ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... and recombinant mouse CXCL4 (500ng/mouse; R&D Systems, Minneapolis, MN, USA). Sham treated mice received an equal volume of PBS i.t ...
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bioRxiv - Developmental Biology 2021Quote: The following antibodies were used for immunofluorescence: goat anti-platelet derived growth factor-alpha (PDGFRA, 1:1000, AF1062, R&D Systems), rat anti-PDGFRA (PDGFRA ...
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bioRxiv - Bioengineering 2020Quote: Expression levels of cell secreted Osteoprotegerin and Vascular Endothelial Growth Factor (VEGF) was examined via ELISA (R&D Systems, Minnesota, USA). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... cells were treated on either brain or vascular channel with TNF-α (Tumor Necrosis Factor-α, R&D Systems; 210-TA). The treatment was initiated after the formation of a confluent monolayer at ∼5 days in culture ...
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bioRxiv - Bioengineering 2020Quote: ... and the unbound growth factors in the supernatant were measured using enzyme-linked immunosorbent assay (ELISA; R&D Systems, Minneapolis, MN). For the other loading procedure (G3) ...
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bioRxiv - Immunology 2020Quote: ... 511 or 111 coated filters and stimulated for 4 h with 5nM tumor necrosis factor-α (TNF-α; R&D Systems), then human monocytes or monocyte Hoxb8 cells were added ...
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bioRxiv - Cancer Biology 2022Quote: ... human cytokines (1 ng/ml stem cell factor, 100 ng/ml thrombopoietin, 13.5 ng/ml interleukin 9, 10 ng/ml interleukin 6; all cytokines from R&D Systems), and 1XPenicillin-Streptomycin (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... For hCO the neural medium was supplemented with the growth factors EGF (20 ng ml−1, R&D Systems, 236-EG) and FGF2 (20 ng ml−1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The dissociated tissue pellet was resuspended in cold Cultrex® Reduced Growth Factor BME type 2 (Cultrex BME) (R&D systems) and 25 mL drops per well were plated on pre-warmed 48-well plates ...
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bioRxiv - Bioengineering 2023Quote: ... Fibroblasts were treated with or without recombinant human transforming growth factor beta 1 (TGF-β1) protein (240-B, R&D Systems) at 1.25 ng/mL ...
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bioRxiv - Biophysics 2023Quote: ... about 15.000 cells per well were resuspended in Cultrex UltiMatrix Reduced Growth Factor (RGF) Basement Membrane Extract (BME) (R&D Systems) and seeded in pre-heated low adhesion 24-well plates (GreinerBio) ...
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bioRxiv - Neuroscience 2020Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
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bioRxiv - Biochemistry 2021Quote: Human anti-SP IgG or ACE-2 Fc was mixed with recombinant SARS-CoV-2 spike RBD His tag (R&D Systems) or recombinant SARS-CoV-2 spike RBD (N501Y)-His tag (Sino Biological ...
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bioRxiv - Microbiology 2021Quote: ... The Hoc proteins each eluted as a single monomeric peak and were run on reducing SDS-PAGE and verified by anti-His western (R&D Systems) (Supplementary Fig.3) ...
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bioRxiv - Biochemistry 2021Quote: Human CD38 (P28907, a soluble fragment Val43-Ile300 with 6x-His tag at C terminus) was purchased from R&D Systems (USA). It was diluted to final 0.01 mg/ml in 100 mM HEPES/NaOH buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... A biotin conjugated anti-His-tag antibody (NB100-63172, Bio-Connect) was added followed by a Streptavidin-HRP (DY998, R&D Systems). For a colormetric detection ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Bioengineering 2021Quote: ... dickkopf-related protein-1 (Dkk-1) and transforming growth factor beta 1 (TGF-β1) were obtained from R&D systems (Minneapolis, MN) and performed on conditioned media from hMSCs grown on GelMA and OCM-GelMA microcarriers in RWV bioreactors.
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bioRxiv - Bioengineering 2021Quote: ... wells were washed and the growth factors remained bond to the heparin conjugates were detected by incubation with the corresponding antibody (R&D systems) followed by a peroxidase-labeled secondary antibody (Jackson ImmunoResearch) ...
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bioRxiv - Cell Biology 2020Quote: ... serum starved at 37°C in MV2 medium (containing no growth factors) 3 hrs before stimulation with VEGFA164 (50 ng/ml; R&D Systems), followed by fixation in 3% PFA for 3 min ...
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bioRxiv - Microbiology 2022Quote: ... cell media was replaced with media containing the compound and 20 ng/mL tumor necrosis factor alpha TNF-α (R&D Systems).
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bioRxiv - Neuroscience 2019Quote: ... for 24h or with 10 ng/mL tumor necrosis factor alpha and 10ng/ml IL1-ß(R&D Systems; 401-ML-005) for 24h ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Intestinal organoids were grown in base culture media (Advanced DMEM/F12 media, HEPES, GlutaMax, penicillin, and streptomycin) supplemented with growth factors (EGF, Noggin, R-spondin, R&D Systems), B27 (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: ... normal T cell expressed and secreted (RANTES) and tumor necrosis factor alpha (TNF-α) by ELISA (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Intestinal organoids were grown in base culture media (Advanced DMEM/F12 media, HEPES, GlutaMax, penicillin, and streptomycin) supplemented with growth factors (EGF, Noggin, R-spondin, R&D Systems), B27 (Life Technologies) ...
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bioRxiv - Immunology 2021Quote: ... IL-6 and tumour necrosis factor (TNF) in the supernatant were analysed by enzyme-linked immunosorbent assay (ELISA; DuoSet, R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... either vehicle (PBS) or candidate factors were added at the following concentrations: BDNF (5ng/mL, R&D Systems, Cat# 248-BDB-005), GDNF (5ng/mL ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were minced and digested with collagenase to single cells before embedded in reduced growth factor extracellular matrix (Cultrex BME2, R&D Systems). Selective isolation of ductal stem cells was achieved using Isolation Medium (IM ...
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bioRxiv - Systems Biology 2023Quote: ... on plates coated with 10 µg/cm2 Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane (R&D Systems #3434-010-02). Cells were cluster-passaged every 3-4 days with 0.5 mM EDTA and seeded into medium supplemented with 10 µM Y-27632 dihydrochloride (Bio-Techne #1254/10 ...
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bioRxiv - Genetics 2023Quote: ... hiPSCs were plated onto Cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 25 mL flask (cat#353018 Falcon ...
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bioRxiv - Developmental Biology 2023Quote: ... washed 2x with PBS and seeded at a density 50 000 cells/50 μl reduced growth factor BME Type II (CultrexTM, R&D systems) in a 24-well cell culture plate (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... into a 96-well plate pre-coated with non-diluted Cultrex reduced growth factor base membrane extract (RGF-BME) (R&D Systems). The images of the network structure were captured by IncuCyte S3 real-time imager every 2 h at 4X magnification ...
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bioRxiv - Cell Biology 2024Quote: ... crypts per isolated from murine intestine and cultured in domes of Cultrex Pathclear Reduced Growth Factor Basement Membrane Extract (3533-005, R&D Systems), covered by ENR media comprised of Advanced DMEM/F12 (12634028 ...