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Citations for R&D Systems :
401 - 450 of 2291 citations for Gem Associated Protein 6 GEMIN6 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Cells were incubated with CD22-Fc protein (R&D Systems) in PBS for 30 min on ice and washed with PBS twice to remove the unbonded protein ...
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bioRxiv - Developmental Biology 2024Quote: ... BMP4 protein was purchased from R&D Systems (5020-BP). LDN193189 was provided by Dr ...
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bioRxiv - Physiology 2024Quote: ... Recombinant human TGFβ2 protein was ordered from R&D Systems and reconstituted in sterile 4 mM HCl with 0.1% BSA at 20 ug/mL.
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bioRxiv - Microbiology 2024Quote: ... Soluble recombinant human PDGFRα-Fc fusion protein (R&D Systems) and heparin sodium salt (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant mouse TREM2 protein (R&D Systems; cat. #1729- T2) was reconstituted according to the manufacturer specifications and diluted to a range of 0-10 ng/mL for the standard curve ...
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bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3 times with washing buffer and a horseradish peroxidase-streptavidin (HRP-Strep) complex (R&D Systems DY998) diluted 1:25 in reagent diluent was added to the plates and incubated for 40 minutes with agitation ...
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bioRxiv - Cell Biology 2020Quote: ... and IL-6 (assay detection range 9.38-600 pg/ml) were purchased from R&D Systems (Minneapolis, MN, USA) and were used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The specificity of staining was confirmed by staining with rat IgG (ref:6-001-F, R&D Systems, Biotechne) or rabbit IgG (ab27478 ...
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bioRxiv - Developmental Biology 2021Quote: ... into chondroprogenitor cells (6 days; 20 ng/ml BMP4 [cat. num. 314-BP-010CF; R&D Systems, Minneapolis, MN]). Mesodermal differentiation medium had a base of Iscove’s Modified Dulbecco’s Medium ...
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bioRxiv - Neuroscience 2021Quote: ... Supernatants were collected and assayed for IL-6 and TNF-α by ELISA Kits (R&D Systems, Minneapolis, MN).
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bioRxiv - Bioengineering 2020Quote: ... supernatants were collected and IL-6 and TNF-α levels were determined using DuoSet ELISA kits (R&D Systems) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Mouse IL-6 was measured in cell supernatants using a DuoSet ELISA kit per manufacturer instructions (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... Mouse IL-6 was detected from cell culture supernatants by ELISA according to the manufacturer’s instructions (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 in cell supernatants was determined by ELISA following the manufacturer’s instructions (R&D Systems). Results were read in a Synergy Mx plate reader (BioTek).
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bioRxiv - Physiology 2022Quote: ... mouse IgG2a kappa (eBM2a) (eBioscience™, 14-4724-82) and the rat IgG (R&D Systems, 6- 001-F).
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bioRxiv - Physiology 2024Quote: ... Then 6 hours later NRVMs were treated with PBS or 100ng/mL IGF1 (291-G1-01M; R&D Systems) to elicit physiological hypertrophy for 48 hours prior to cell harvesting for experiments ...
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bioRxiv - Immunology 2022Quote: ... PPS14-TT together with recombinant IL-6 (500 ng/adult and 100 ng/neonate (R&D systems, Minneapolis, MN) was emulsified with aluminum hydroxide ...
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bioRxiv - Immunology 2024Quote: ... The level of mature IL-1β and IL-6 in the PLF was determined by ELISA (R&D Systems).
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bioRxiv - Immunology 2024Quote: ... IL-6 and IL-1β levels released by treated macrophages were determined using DuoSet ELISA kits (R&D Systems) per the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... Cytokine levels for IL-6 and MCP-1 were determined by ELISA according to manufacturer’s instructions (R&D Systems).
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bioRxiv - Neuroscience 2024Quote: ... or 6 h of incubation) was measured using the human CCL11/Eotaxin kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The SKP1 containing SCF complex was reconstituted by utilizing stoichiometric amount of the following recombinant proteins: Recombinant Human CUL1/RBX1 Neddylated Complex Protein (R&D systems, E3-411-025), Recombinant Human FBXO7 GST (N-Term ...
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bioRxiv - Microbiology 2021Quote: ... the following Abs were used: polyclonal sheep anti-human CD47 (AF4670) and sheep IgG HRP-conjugated Ab (HAF016) from R&D system; mouse anti-HA mAb (16B12) ...
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bioRxiv - Immunology 2022Quote: ... while A29L antigen was detected with rabbit polyclonal NR-627 primary (BEI Resources) and an anti-rabbit IgG-HRP (R&D Systems). Data analysis was performed using Compass software (ProteinSimple/Bio-Techne).
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bioRxiv - Molecular Biology 2023Quote: ... and bound biotinylated ACE2 and RBD in the presence or absence of SP-A was detected by incubating with Streptavidin-HRP (VANC00B, R&D Systems) for 1 h at room temperature and developed as described above ...
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bioRxiv - Biochemistry 2023Quote: ... plates incubated with biotinylated antibody were washed and incubated at 37°C for 1 hr with a 1/200 dilution of Streptavidin-HRP (Catalog #890803, R&D systems). Plates were then washed and developed using 100□μl/well SureBlue TMB (3,3′ ...
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bioRxiv - Immunology 2024Quote: ... the GR-β blot was washed 3 times with TBS-T and incubated with anti-rabbit HRP-conjugated secondary IgG (1:2000 dilution; cat. no. HAF008, R&D Systems) in 5% w/v non-fat milk (cat ...
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bioRxiv - Immunology 2024Quote: ... at 1 ug/ml for 1 hour at room temperature followed by incubation with anti-goat IgG HRP (R&D Systems #HAF109) and stained using DAB (brown ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then rinsed with PBS and chromogenic staining was performed using Rabbit VisUCyte HRP Polymer-DAB Cell & Tissue Staining Kit (VCTS003, R&D Systems) following manufacturer’s instructions and counterstained with hematoxylin (Millipore Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... The anti-artemin monoclonal antibody and control IgG isotype antibodies were purchased from R&D Systems, reconstituted in sterile PBS solution ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-VE-cadherin antibody (AF1002) and anti FOXF1 antibody (AF4798) were purchased from R&D system) ...
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bioRxiv - Bioengineering 2024Quote: ... The following primary antibodies and secondary antibodies were used (staining durations provided): CYR61 (R&D Systems, Minneapolis ...
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bioRxiv - Cancer Biology 2023Quote: ... The capture AXL antibody was generated by conjugating a primary AXL antibody (R&D systems MAB154) onto magnetic beads (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... Anti-Dig-POD antibody (1:100) and primary antibody (anti-Otx2, R&D Systems, Cat#: AF1979) in 100 µl of TNB were added to samples ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-VE-cadherin antibody (AF1002) and anti FOXF1 antibody (AF4798) were purchased from R&D system. Anti S1PR1 antibody was from Sigma-Aldrich (SAB4500687 ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were treated for 2 days (days 6, 7) with 50 ng/ml sonic hedgehog (SHH; R&D Systems), 2 μM dorsomorphin (DSM ...
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bioRxiv - Immunology 2020Quote: ... IL-10 and IL-6 were measured by sandwich ELISA using the Duo Set ELISA kit (R&D Systems, UK) (23 ...
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bioRxiv - Cell Biology 2020Quote: ... retinae from C57bl6 and Cx3cr1GFP/GFP animals (n = 6) were incubated as above with fractalkine (200ng/ml, R&D Systems) or PBS for 2 hours at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and M2d MΦs from stimulation with and 50 ng/mL recombinant human IL-6 (R&D Systems; Minneapolis, MN, USA) and 25 ng/mL recombinant human leukemia inhibitory factor (LIF ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-6 in cell culture supernatant were determined using the mouse DuoSet kit (R&D Systems, Minneapolis, MN). All assays were performed according the manufacturer’s supplied protocol ...
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bioRxiv - Neuroscience 2023Quote: ... IL-6 and IL-8 levels in conditioned media were quantified using Quantikine immunoassays (HS600C and D8000C, R&D systems). GFAP was measured in CM using Human GFAP DuoSet ELISA (R&Dsystems ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed for RNA analysis and supernatants were analysed for IL-6 content by ELISA (DuoSet, R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... and IL-6 levels were quantified in BALF and lung tissues using commercially available DuoSet ELISA kits (R&D Systems) according to manufacturer’s instructions and published protocols 22 ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng/mL mouse IL-3 (403-ML) and 10 ng/mL mouse L-6 (406-ML) (R&D Systems). Total RNA was extracted using the RNeasy Mini kit (QIAGEN ...
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bioRxiv - Cell Biology 2024Quote: To measure IL-6 in conditioned media a sandwich ELISA method (Quantikine® ELISA Immunoassay, R&D Systems, Abingdon, UK) was employed following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... BMP4 recombinant protein (R&D Systems, Catalog No. 314-BP-050) was diluted in 15% pluronic gel (prepared in PBS ...
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bioRxiv - Immunology 2020Quote: ... IL-15Rα alone (IL-15Rα Fc chimera protein, R&D Systems) or IL-15Rα pre-complexed with IL-15 (Peprotech ...
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bioRxiv - Cancer Biology 2022Quote: ... and Recombinant Human CXCL1/GROα Protein (R&D Systems, 275-GR).
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant Human CCL20/MIP-3α Protein (R&D Systems, 360-MP), IL-8 Monocyte Recombinant Human Protein (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant human SUMO1-AMC and SUMO2-AMC proteins (R&D Systems) were diluted in parallel to 500 nM in Assay buffer (Tris HCl pH 8.0 50 mM ...