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Citations for R&D Systems :
401 - 450 of 595 citations for 6 Chloro 3 formylchromone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... serum was collected 3 hr after injection and cytokines were quantified by ELISA (R&D systems) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... and the Human IL-29/IL-28B (IFNλ1/3) DuoSet ELISA (DY1589, R&D Systems, USA) kit according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... FL5.12 cells were cultured in the presence of 10 ng/ml IL-3 (R&D Systems). Cell cycle exit in FL5.12 was achieved by washing the cells three times with PBS to remove IL-3 from the cells and by placing the cells in IL-3 free culture media for 48 h ...
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bioRxiv - Neuroscience 2024Quote: ... coated plates in the expansion medium (N2B27) supplemented with 3 µM CHIR (R&D System, 4423), 0.5 µM PMA (Cayman Chemical ...
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bioRxiv - Cell Biology 2020Quote: ... mOSE cells (1×10^6) were first plated as monolayer cultures 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). Four days after the addition of TGFB1 ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-1β and TNFα and IL-6 in cell culture supernatants or serum were measured using Human IL-1 beta/IL-1F2 DuoSet ELISA (DY201, R&D Systems), Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401 ...
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bioRxiv - Immunology 2019Quote: ... from wild type and Irak1−/− mice were prepared by differentiation for 6 days in the same culture medium containing macrophage colony-stimulating factor (M-CSF; 60 ng/mL, R&D Systems). LPS was from Alexis Biochemicals (Salmonella minnesota R595 TLR grade ...
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bioRxiv - Pathology 2019Quote: Supernatants were tested for 6 analytes (TNFa, IL-6, IL-8, IL-10, IL-1b, IL-1a) on a custom Human magnetic Luminex plate (R&D systems LXSAHM ...
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bioRxiv - Immunology 2019Quote: ... anaesthetized C57BL/6 mice were first injected with 50 µl anti-LYVE1-Alexa Fluor 488 antibody (20 µg/ml, R&D Systems) into the rear right hock ...
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bioRxiv - Biochemistry 2019Quote: ... Cytokines (IL-6 and KC) were quantified by an enzyme-linked immunosorbent assay (Quantikine; R&D Systems Europe, Abingdon, Oxon, United Kingdom).
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bioRxiv - Neuroscience 2020Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
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bioRxiv - Immunology 2021Quote: Mature macrophages (n = 6 donors) were stimulated for 48 hours with complete medium containing IFN-γ (20 ng/mL, R&D Systems) and LPS (100 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... The expression of PD-L1 and IL-6 from bronchoalveolar lavages was quantitated following the protocols of ELISA kits: Human IL-6 Quantikine ELISA Kit and Human/Cynomolgus Monkey B7-H1/PD-L1 Quantikine ELISA Kit from R&D Systems.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and the supernatant was used for the quantification of IL-1β e IL-6 cytokines using commercial kits (R&D System®).
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bioRxiv - Pathology 2022Quote: ... The concentrations of IL-6 and VEGFA were determined using an enzyme-linked immunosorbent assay (Mouse IL-6 ELISA kit, ThermoFisher; Mouse VEGF-A DuoSet ELISA kit, R&D Systems). All media was collected two days following media change ...
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bioRxiv - Pathology 2020Quote: ... recombinant human Cadherin 6 Fc chimera protein (~130 kD) or recombinant mouse Cadherin 6 protein (~90 kD) was used to generate a standard curve (R&D Systems catalog # 2715-CA or 6826-CA ...
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bioRxiv - Pathology 2020Quote: Cytokines in 200 microgram kidney lysate (pooled from 5-6 samples with equal amount of protein) were detected with the Proteome Profiler Array (ARY028; R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... Plasma interleukin (IL)-6 and lipopolysaccharide-binding protein (LBP) levels were measured by commercially available enzyme-linked immunosorbent assay (R&D Systems, Minneapolis ...
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Genomics 2022Quote: Three million cells from each triplicate were seeded separately in 6 well-plates in fresh RPMI media and left unstimulated or stimulated with universal interferon alpha (200U/ml) (R&D systems) for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... IL-6 and tumour necrosis factor (TNF) in the supernatant were analysed by enzyme-linked immunosorbent assay (ELISA; DuoSet, R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: Confluent T24 cells transfected with control and pacsin 2 RNAi in 6-well plates were treated with 10 ng/ml of recombinant human EGF (236-EG, R&D systems) in RPMI-1640 medium and incubated at 37℃ ...
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bioRxiv - Microbiology 2022Quote: Distal ileal TNF-α and IL-6 were quantified using the mouse TNF-α enzyme-linked immunosorbent assay [ELISA] Ready-SET-Go kit (eBioscience; San Diego, CA, USA) and the Mouse IL-6 DuoSet ELISA (R&D Systems) respectively ...
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bioRxiv - Neuroscience 2022Quote: ... The pre-immobilized beads were then incubated with 50 nM recombinant mouse SorCS1 ectodomain tagged with a C-terminal 6-His tag (SorCS1-His, Cat# 4395-SR-050, R&D systems) in ECS for 2 h at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by ELISA using the DuoSet ELISA Kits (DY210-05, DY201-05, and DY206-05) from R&D Systems per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The cytokine releases were evaluated by ELISA in cell supernatants or in peritoneal fluids (IL-1β, IL-12, IL-6, TNFα, TGFβ, IL-10 BD Biosciences, R&D Systems). 8 isoprostane ...
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bioRxiv - Immunology 2023Quote: ... 3000 – 5000 ASCs per well were cultured in sterile 96-well tissue culture plates (Falcon) in the presence of 20 ng/mL IL-6 (R&D Systems), 100 ng/mL APRIL (R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Developmental Biology 2022Quote: Cells were dissociated with TrypLE on day 6 and 8 of CMEC and PMEC protocol and stained with VE-Cadherin PE-conjugated Antibody (R&D Systems). Then VEC+ cells were sorted using FACSAria III ...
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bioRxiv - Microbiology 2023Quote: ... Mice were sacrificed 24h post-infection and n=7 were analysed for bacterial load and IL-6 and KC using ELISA (R&D systems). A further n=3 animals were analysed using histology ...
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bioRxiv - Immunology 2024Quote: ... or 100 ng/ml N-palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cysteinyl-[S]-seryl-[S]-lysyl-[S]-lysyl-[S]-lysyl-[S]-lysine (Pam3CSK4) for 6 h and IL6 secretion measured by ELISA (R&D Systems).
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bioRxiv - Cell Biology 2024Quote: Extracellular conditioned media from differentiated 3T3-L1 cells pretreated with or without palmitate and UNC0642 were quantified using mouse IL-6 Quantikine ELISA kit M6000B from R&D Systems as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... For activation endothelial cells were incubated with hMDM conditioned media for 6 h in absence or presence of TNFRII/TNFRSF1B-Fc chimera protein (726-R2-050, R&D Systems) at 1 µg/ml.
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bioRxiv - Cancer Biology 2024Quote: ... IL-6 concentrations in tissue lysates and CAF supernatants were measured using a commercially available kit (Human IL-6 DuoSet ELISA, R&D Systems). SAA1 concentrations in patient sera were measured using a commercially available kit (Human SAA1 DuoSet ELISA ...
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bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-6 (MAB9540, clone 973132), IL-12p70 (MAB611R, clone 24945R), and MCP-1 (AF-279, polyclonal) obtained from R&D Systems. Surface enhanced Raman scattering (SERS ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatants were transferred to a new eppendorf tube and used for measuring the levels of cytokines using the Mouse TNF-alpha Quantikine ELISA kit and the Mouse IL-6 Quantikine ELISA kit (R&D systems), following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... or Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3) was placed in the upper and lower chambers ...
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bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL human Fms-related tyrosine kinase 3 ligand (Flt3L, #308-FK, all R&D Systems), 2 mM L-GlutaMAX (Gibco #35050-038) ...
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bioRxiv - Microbiology 2021Quote: ... organoids were washed twice with PBS and resuspended in Cultrex 3-D Culture Matrix (R&D Systems) for 30 min before organoid medium (as described above ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was quantified using Human Dkk-3 DuoSet Solid Phase Sandwich ELISA (DY1118, R&D Systems, MN), per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and the respective growth factors and small molecules (8 µM GSK-3 inhibitor CHIR99021 (R&D Systems), 200 ng/mL FGF9 (fibroblast growth factor 9 ...
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bioRxiv - Cancer Biology 2021Quote: We cultured 3*105 million freshly enriched DCs in 2ml Dendritic Cell Base Media (R&D systems) plus 10% FBS ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used were rabbit anti-Human Phospho-Smad2/3 (R&D Systems MAB8935, 1:500 dilution), goat anti-histone (Abcam ab12079 ...
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bioRxiv - Bioengineering 2024Quote: ... which was supplemented with 3 ng/mL human activin A (R&D Systems, Inc., Minneapolis, MN, USA), 1 ng/mL human bone morphogenetic protein 4 (Bmp4 ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from an overnight agar culture and incubated with 3 mM HMGB1(R&D systems) diluted with protein block buffer (Dako ...
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bioRxiv - Immunology 2023Quote: ... or with 10 µl biotinylated irrelevant antigen (3 nM final concentration; R&D Systems cat# AVI10538-050) for 20 minutes at room temperature in 96 half area white microplates (Corning cat# CLS3693) ...
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bioRxiv - Cell Biology 2023Quote: Immunohistochemistry was performed with the following antibodies: Cleaved Caspase 3 (rabbit, R&D Systems, MAB835, 1:100), Hopx (mouse ...
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bioRxiv - Bioengineering 2024Quote: ... The FL5.12 cell culture media was also supplemented with 100 ng/ml IL-3 (R&D Systems). L1210 cells were obtained from ATCC ...
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bioRxiv - Cancer Biology 2024Quote: ... The following primary antibodies were used: rabbit anti active caspase 3 (1:200; R&D Systems AF835), and rabbit anti-Ki67 (1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested using the Quantikine human IL-6 (sensitivity < 5 pg/mL) ELISA kit (R&D Systems, Inc., Minneapolis, MN) according to manufacturer’s recommended conditions ...