Labshake search
Citations for R&D Systems :
4401 - 4450 of 6141 citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... media in the wells was either with or without 10% FBS and 1μg/mL secreted N-SLIT2 (R&D Systems, 8616-SL-050). The cells were incubated in the Matrigel-coated inserts for overnight in the incubator ...
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC, Manassas, Virginia; Cat #30-2002) supplemented with 10% fetal bovine serum (FBS) (R&D Systems, Minneapolis, MN), 1% penicillin/streptomycin (PS ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were kept in enriched N-2 medium with 10 ng/ml of basic fibroblast growth factor (R&D systems, Minneapolis, MN, USA) added every 24 h and medium changed every alternate day to keep the cells in an undifferentiated and proliferative state ...
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bioRxiv - Pathology 2020Quote: ... Cells were cultured to 70-80% confluence and then treated with vehicle (4mM HCl with 0.1% bovine serum albumin) or 10 ng/mL TGF-β1 (Cat#: 7754-BH-005, R&D Systems, Minneapolis, MN) or 10 ng/mL TNF-α (Cat#210-TA-020 ...
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bioRxiv - Microbiology 2021Quote: ... were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (Bio-Techne, R&D systems, Minneapolis, MN, USA) and 1% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then seeded onto 24 well plates (106 cells/well) with or without 10 ng/mL murine IFN-γ (R&D Systems). Sixteen hours later ...
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bioRxiv - Microbiology 2021Quote: ... LAD2 cells were prior-treated with 0.25% trypsin (without EDTA) for 10 min at 37 °C or prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 2 h at 37 °C before the incubation with Spike-RBD protein.
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bioRxiv - Cell Biology 2022Quote: ... 100μg of GST-NEDD4△52 was bound to 10 μl Glutathione sepharose resin and incubated with 50 nM ubiquitin activating enzyme UBA1 (R&D Systems, E305025), 750 nM ubiquitin conjugating enzyme UBCH5B (R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were allowed to recover for 5 min and were treated with 10 ng/ml PDGF-AA (PDGFRα homodimer cells) or PDGF-BB (PDGFRα/β heterodimer and PDGFRβ homodimer cells) ligand (R&D Systems) for the indicated amount of time ...
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bioRxiv - Cell Biology 2023Quote: ... PDGFRα homodimer cells and PDGFRβ homodimer cells at ∼60–70% confluence or PDGFRα/β heterodimer cells at ∼70-80% confluence were serum starved for 24 h in HITES medium and stimulated with 10 ng/ml PDGF-AA (PDGFRα homodimer cells) or PDGF-BB (PDGFRα/β heterodimer and PDGFRβ homodimer cells) ligand (R&D Systems) for the indicated length of time ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were cultured in N2B27 with 10 ng/ml mouse basic fibroblast growth factor (bFGF) recombinant proteins (R&D systems #313FB025/CF). On Day 2 ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: VeroE6 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (Bio-Techne, R&D systems, Minneapolis, MN, USA) and 1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Harvested cells were cultured in RPIM 1640 medium containing 20% porcine serum and 10 ng/mL recombinant porcine granulocyte-macrophage colony-stimulating factor (GM-CSF, R&D Systems, UK) to differentiate mature bone marrow-derived macrophage (BMDMs ...
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bioRxiv - Immunology 2023Quote: iBMDMs were maintained in Dulbecco’s Modified Eagle Medium (DMEM; HyClone Cytiva, Cat no. SH30243.01) supplemented with 10% fetal bovine serum (FBS) (R&D Systems, Cat no. S11550). iBMDMs were passaged once they reached 70–90% confluency ...
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bioRxiv - Cancer Biology 2023Quote: ... TUBO and TUBO/Crimson were cultured using high-glucose DMEM base medium with 10% heat-inactivated fetal bovine serum (FBS) (R&D Systems, S11150H), 10% NCTC-109 medium (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Parallel experiments included the addition of a neutralizing anti-IL-15 MoAb (10 mg/ml) (mab247, mouse IgG1, R&D systems, Abingdon, UK). Alternatively ...
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bioRxiv - Immunology 2024Quote: Isolated B cells were activated at a concentration of 2E6 cells/mL with 10 ng/mL rBAFF (R&D systems 8876-BF-01) and specified combinations of 5 µg/mL (unless otherwise stated ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-mouse Lyve1 (1:500, #DP35135P, Acris Antibody GmbH, Herford, Germany) and sheep anti-mouse Trem2 (1:50, #AF1729, R&D Systems), and rabbit anti-mouse Ki67 (1:200 ...
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bioRxiv - Immunology 2021Quote: ... This reaction was carried out by incubating 1 μM of the quenched AZP probe with 1 μg/mL of recombinant MMP14 (918-MP-010, R&D Systems), ADAM17 (2978-AD-10 ...
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bioRxiv - Cell Biology 2020Quote: ... 832/13 cells were starved for 2 h, and treated with insulin-like growth factor 1 (IGF-1, NIH repository) or hepatocyte growth factor (HGF, R&D Systems) for various times.
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bioRxiv - Neuroscience 2021Quote: ... The cells were immunostained with rat anti-GFP (1:2000, GF090R; Nacalai Tesque) and goat anti-TrkB (1:250, AF1494; R&D Systems). Cells were extensively washed and incubated with appropriate secondary antibodies at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: Goat polyclonal antibodies against NEGR1 (AF5394) used for immunocytochemistry (IC: 1:100) and immunohistochemistry (IHC: 1:1000) were from R&D systems. Rabbit polyclonal antibodies against NEGR1 (ab101809 ...
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bioRxiv - Neuroscience 2021Quote: ... The sandwich layout of this TREM2 MSD assay from the bottom to the top is (1) Capture-antibody (1 μg/mL; BAF1729, R&D Systems), (2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Lung lobes were incubated with primary antibody (anti-VWF, rabbit, 1:100, F3520, Sigma and anti-EMCN, goat, 1:200, AF4666, R&D Systems), in 5% donkey serum and 0.5% Triton X-100 in phosphate buffered saline (PBST ...
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bioRxiv - Cell Biology 2020Quote: ... cells were refreshed in DMEM + 1% (v/v) PS + 1% (v/v) B-27 + 50 ng/ml EGF (Cat. No. 236-EG, R&D System) + 25 ng/ml FGF7 for 4 days ...
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bioRxiv - Bioengineering 2022Quote: ... A low-serum modified cell line was previously established where PANC-1 cells were cultured in DMEM supplemented with 2% FBS and 1% Insulin-Transferrin-Selenium Media Supplement (ITS, R&D Systems) (“DMEM-ITS”)45 ...
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bioRxiv - Genetics 2022Quote: ... Membranes were probed by incubation for 1 hour at room temperature with antibodies against LBP (#AF870-SP, R&D Systems, 1:2,000), DDK (#A8592 ...
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bioRxiv - Immunology 2022Quote: ... 1:1000 diluted in PBS followed by a secondary fluorochrome anti-rabbit NorthernLights NL637-conjugated antibody (NL005; R&D systems, 1:200). Sections were then mounted with Fluormount G containing DAPI (Southern Biotech) ...
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bioRxiv - Neuroscience 2022Quote: ... The neurofilament marker SMI32 (mouse monoclonal, 1:1000; Sternberger Monoclonals) and a goat anti-osteopontin antibody (OPN, 1:1000; R&D Systems) were used to label alpha RGCs ...
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bioRxiv - Microbiology 2024Quote: ... and treated with a blocking solution (dPBS +Ca2 +Mg2 with 2% (v/v) FBS) containing an anti-hTIM1(1:50-1:100, AF1750, R&D Systems) antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... or N-acetylglucosamine for 1 h in plates previously coated with either S protein (1 µg/ml) or RBD (50 ng/ml, VANC00B, R&D Systems). As a control ...
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bioRxiv - Biochemistry 2023Quote: ... plates incubated with biotinylated antibody were washed and incubated at 37°C for 1 hr with a 1/200 dilution of Streptavidin-HRP (Catalog #890803, R&D systems). Plates were then washed and developed using 100□μl/well SureBlue TMB (3,3′ ...
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bioRxiv - Neuroscience 2023Quote: ... Neuropilin-1 was visualized using a goat polyclonal anti-NRP-1 antibody at a 15 µg/mL concentration (AF566; R&D Systems) followed by an Alexa Fluor® 647 conjugated donkey anti-goat monoclonal antibody at a 1:1000 concentration (ab150135 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... bEFNB2 expressing CHO cells were passaged in puromycin (1-5 ug/mL) for 1 week and then stained with EphB3-Fc (5667-B3-050; R&D Systems), a natural ligand of EFNB2/3 ...
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bioRxiv - Cell Biology 2020Quote: ... α-fetoprotein (R&D Systems, MAB1368, diluted at 1/100), and CYP3A4 (SANTA CRUZ BIOTECHNOLOGY ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-Klf5 (1:500, R&D Systems, AF3758), rabbit anti-Cldn1 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Sox10 (1:800, R&D Systems, MAB2864), rabbit anti-Sox9 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-GAPDH (1:10,000, #MAB5718, R&D Systems)
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bioRxiv - Developmental Biology 2021Quote: ... goat anti-GATA6 (1:200, AF1700, R&D Systems), rabbit anti-Laminin (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... goat anti-T (1:100, AF2085, R&D systems), mouse anti-DAB2 (1:100 ...
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bioRxiv - Developmental Biology 2021Quote: ... goat anti-SOX9 (1:1000, AF3075, R&D Systems), rat anti-LAMB1 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... and TGF-β (1 ng/mL) (R&D Systems). The medium 1 was changed every day ...
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bioRxiv - Developmental Biology 2020Quote: ... goat anti-Nurr1 (R&D systems AF2156, 1:200), mouse anti-Meis2 (Sigma WH0004212M1 ...
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bioRxiv - Developmental Biology 2020Quote: ... goat anti-Isl1 (R&D systems AF1837, 1:100), mouse anti-Nr2e3 (R&D systems PP-H7223,1:100) ...
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bioRxiv - Developmental Biology 2020Quote: ... goat anti-Otx2 (Af1979, R&D Systems,1:400), and mouse IgG2b anti-HNF-6 (sc-376308 ...
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bioRxiv - Developmental Biology 2021Quote: ... goat anti-SOX2 (R&D Systems; AF2018; 1:250), rabbit anti-NKX2.1 (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... goat anti-SOX2 (R&D Systems, AF2018; 1:500), goat anti-FOXA2 (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-BRG1 (1:250, R&D systems, MAB5738), mouse anti-FOXM1 (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-mTrkB (#AF1494, RRID:AB_2155264, 1:1000, R&D Systems), anti-GSK3β (#9315 ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-HBEGF (1:200, AF259; R&D Systems), mouse anti-HBEGF (1:500 ...