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Citations for R&D Systems :
4051 - 4100 of 4586 citations for Cow Polypyrimidine tract binding protein 1 PTBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: ... The membranes were probed with an anti-MHC antibody (1:100, R&D Systems, Cat.# MAB4470) in blocking buffer (5% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Wnt3a was supplemented only on day 1 (25 ng/ml, R&D Systems, #5036-WN/CF). Thereafter ...
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bioRxiv - Cell Biology 2024Quote: Primary antibodies used: goat anti-CD31 (1:500, Catalog # AF3628, R&D Systems, Inc. Minneapolis, MN). Isolectin b4 (1:250 ...
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bioRxiv - Cell Biology 2024Quote: ... and Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody (1:100, NL009, R&D system) respectively ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 ng/mL human FGF basic/FGF2/bFGF (R&D Systems #233-FB-025/CF). After passage one ...
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bioRxiv - Cell Biology 2024Quote: ... 0.2mM ascorbic acid and 1 ng/mL recombinant human FGF (R&D system, 233-GMP-025). The adherent cells were split by trypsinization at 80% confluency and frozen at passage 2 (50% αMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% penicillin-streptomycin and 50 ng/mL recombinant human GM-CSF (R&D Systems 215-MC). M1 macrophage differentiation medium was prepared using M1 macrophage attachment medium supplemented with 100 ng/mL of LPS (Sigma-Aldrich L2630 ...
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bioRxiv - Neuroscience 2021Quote: ... The relative expression profile of 36 human cytokines and chemokines were evaluated using the Proteome Profiler Human Cytokine Array Kit (R&D Systems, ON, Canada). The experiment was performed according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cell lines were purchased from ATCC (Manassas, VA, USA) and confirmed to be mycoplasma free by MycoProbe kit (R&D Systems, Minneapolis, MN, USA). Cells were grown at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: Apical washes and basolateral media samples were clarified by centrifugation and analyzed using the Magnetic Luminex® Performance Assay Human High Sensitivity Cytokine Base Kit A (R&D Systems, Inc). Apical washes were diluted 1:2 ...
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bioRxiv - Immunology 2022Quote: Naive CD4+CD25- T cells from the LNs and spleen of WT or NAC1-/- mice were incubated with the indicated reagents including TGF-β in the CellXVivo™ Mouse Treg Cell Differentiation Kit (#CDK007, R&D Systems) for 5 days.
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were genetically authenticated by the Australian Cancer Research Foundation (ACRF) Cancer Genomics Facility (Adelaide, Australia) and determined mycoplasma-free using the MycoProbe mycoplasma detection kit (CUL001B, R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2019Quote: ... human sputum supernatants or cell supernatants from in vitro experiments were assayed using commercial “duoset” enzyme-linked immunosorbent assay kits (R&D Systems, Abingdon, UK). The MesoScale Discovery (MSD ...
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bioRxiv - Immunology 2021Quote: Multiplexed ELISA on interstitial fluid from matched T and NAT fresh tissues were performed on a Luminex 200 platform (Luminex Inc.,) using custom kits of pre-mixed antibody-coated beads (R&D System Inc., MN), which included the following analytes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-10 were assayed using a Luminex mouse pre-mixed Multi-Analyte kit according to standard instructions (LXSARM-06, R&D System, Minnesota, USA).
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Long Noncoding RNA MALAT1 is Dynamically Regulated in Leader Cells during Collective Cancer InvasionbioRxiv - Cancer Biology 2022Quote: A 3D invasion assay was applied to investigate the lncRNA dynamics in collective cancer invasion with Cultrex© 3D Spheroid Cell Invasion Assay Kits according to manufacturer’s instructions (R&D Systems, Minneapolis, MN). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: PARP activity was analyzed from pulverized liver and gastrocnemius (GSN) muscle utilizing HT Colorimetric PARP/Apoptosis Assay Kit (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions (n=6-8 per group) ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Immunology 2024Quote: Supernatants harvested from mixed lymphocyte cultures established from three mice of each group were tested using commercial assay kits (e.Bioscience, R&D Systems, Bender MD Systems) for the presence of interferon-γ ...
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bioRxiv - Cancer Biology 2023Quote: Human cell line culture supernatants were analyzed using an R&D systems Human Premixed Multi-Analyte Kit (R&D Systems, Inc., Minneapolis, MN, USA) and a Luminex 200 (RRID ...
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bioRxiv - Immunology 2023Quote: Concentrations of CXCL10 in K18-hACE2 mice were measured in serum and lung supernatant collected at euthanasia using the Mouse Magnetic Luminex Assay kit for mouse CXCL10/IP-10/CRG2 (R&D Systems LXSAMSM-01). Both serum and supernatant samples were diluted 1:2 in the kit’s Calibrator Diluent RD6-52 then utilized in the assay procedure provided by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: Protein profiling of the secreted factors from each cell culture was performed using the Proteome Profiler Human XL Cytokine array kit (R&D systems, Minneapolis, MN) according to the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The iPSCs were functionally characterized by checking for their ability to differentiate into the three germ layers (Human pluripotent stem cell functional identification kit, R&D systems, Cat#SC027B) according to the manufactures protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody used was goat IgG anti-IL-1RA (1: 500, AF-280-NA, R&D Systems) and samples were incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... the cells were stimulated with either 1 µg/ml recombinant murine CXCL13 (CXCL13, R&D Systems; Biotechne), 10 µg/ml AffiniPure F(ab’)2 fragment goat anti-mouse IgM (anti-IgM ...
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bioRxiv - Genetics 2021Quote: ... Coverslips were incubated with the primary anti-SOX2 antibody (mouse monoclonal IgG2a, 1:100, R&D Systems), overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated with a mouse anti-zebrafish VEGF antibody (R&D Systems #MAB1247, 1:1,000) and chicken anti-GAPDH antibody (Sigma #AB2302 ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated overnight at 4°C in the following primary antibodies: T-LCK (1:1000 R&D Systems), T-LCK (1:1000 Proteintech) ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were incubated with goat anti-SAA primary antibody (1:500; AF2948, R&D Systems, Minneapolis, MN) overnight at 4 °C and with horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were run in duplicates using the Mouse Magnetic Luminex Assay for IGF-1 (R&D System) and Milliplex Mouse Pituitary Magnetic Assay for GH (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-UCP1 (1:750, R&D Systems, Minneapolis, MN, USA, MAB6158), anti-p62 (1:5000 ...
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bioRxiv - Physiology 2020Quote: Concentration of GLP-1 was determined using the Magnetic Luminex Assay (R&D Systems Inc., Minneapolis, MN) and MAGPIX reader (Luminex Corporation ...
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bioRxiv - Immunology 2019Quote: ... which was either 20% autologous donor sera or 1 g/ml CXCL10 and CXCL12 (R&D Systems). Cell migration was assessed after incubation at 37°C for 4h ...
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bioRxiv - Immunology 2019Quote: ... Primary antibodies used were: goat anti-mouse IL-33 (AF3626, R&D Systems, Minneapolis, MN; 1:1000), rabbit anti-Muc5ac (gift from Dr ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated with capturing anti-TRK antibody (1:1000, #92991S, Cell Signaling or #AF1494, R&D Systems) in carbonate buffer (pH= 9.8 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated for 5 days at 4°C with Car4 primary antibody (1:500, RRID:AB_10013483; R&D Systems), rinsed ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used to immunostain human tissue: PDGFRβ (1:100, goat, R&D Systems), GFAP (1:200 ...
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bioRxiv - Bioengineering 2021Quote: ... To create multiple loading densities a series of concentrations of PD-1 (R&D systems, #8986-PD) or PD-L1 (R&D systems ...
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bioRxiv - Biochemistry 2020Quote: ... Vascular endothelium was stained with a goat antibody against CD31 (R&D Systems, Inc., diluted 1:500) and visualized via a donkey anti-goat antibody conjugated with AlexaFluor-488 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibodies were used as follows: Chicken anti-Nfasc (1:500; Cat #: AF3235, R&D Systems), Mouse anti-AnkG (1:500 ...
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bioRxiv - Cancer Biology 2019Quote: ... EpCAM-APC (Clone CI:A3-1, AbD Serotec [Hercules, CA]) and CCR2-flourescein (Clone 475301, R&D Systems). Prior to Granzyme B staining ...
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bioRxiv - Biochemistry 2022Quote: ... I used 10 ng/ m L recombinant human interleukin 1 (IL-1α; R&D Systems, Minneapolis, MN). All incubations were done at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Human ACE2 was detected using a polyclonal goat anti-human ACE2 antibody (1:500, R&D Systems), a horseradish peroxidase (HRP)-labeled donkey anti-goat antibody (1:5000 ...
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bioRxiv - Immunology 2022Quote: ... 1% penicillin/streptomycin (Cytiva, Marlborough, MA) and 20 ng/mL GM-CSF (R&D Systems, Minneapolis, MN) (DC media ...
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bioRxiv - Cancer Biology 2022Quote: ... TGF-beta 1 (7754-BH-005) and TNF-alpha (210-TA) were obtained from R&D Systems, and the synthetic bacterial lipopeptide Pam3CysSerLys4 was obtained from Calbiochem ...
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bioRxiv - Biochemistry 2021Quote: ... or OKT3 (eBioscience) each 10 μg/ml) or CXCL12/CCL21 (each 1 μg /ml R&D systems) for the indicated time points ...
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bioRxiv - Neuroscience 2022Quote: ... GDNF (10 ng.mL-1, R&D Systems 248-BD/CF, 257-NT/CF and 212-GD/CF) and doxycycline hyclate (2 µg.mL-1 ...
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bioRxiv - Cancer Biology 2022Quote: For immunoblotting the following primary antibodies were used: anti-PTN (1:1000; R&D systems, AF252-PB), anti-p65 (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... goat anti-platelet derived growth factor receptor-α (PDGFRα, 1:200, AF1062. R&D Systems, MN, USA), goat anti-Iba1 (1:200 ...
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bioRxiv - Immunology 2019Quote: ... Intracellular staining for CCL7 was carried out using 1 in 100 biotinylated CCL7 antibody (R&D Systems) and Strepdavidin BV605 (BioLegend ...