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Citations for R&D Systems :
4001 - 4050 of 4111 citations for Mouse Anti Hepatitis C Virus NS4b Antibody 1858 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then subjected to western blot analysis detailed above to detect TAOK2 autophosphorylation by rabbit antibody against pS181 (R&D Systems, PPS037).
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bioRxiv - Physiology 2022Quote: ... or immunofluorescent (IF) staining as indicated below: Antibody against VE-cadherin extracellular domain (WB 1:1000, IF 1:50, R&D Systems, MAB9381), antibody against VE-cadherin intracellular domain (WB 1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Total VEGFR2 expression (0.1% Tx-100 permeabilized cells) was compared to surface level VEGFR2 (non-permeabilized cells) using a biotinylated VEGFR2 antibody (R&D Systems; BT10543). Images were collected using a W1 spinning disk confocal microscope.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Levels of vascular endothelial growth factor (VEGF) were evaluated by sandwich enzyme-linked immunosorbent assay (ELISA) in GL261 cell supernatants using matched antibody pairs from R&D Systems (USA), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... for 1 hour at room temperature followed by overnight incubation with primary antibodies for IL-31RA (15 µg/ml, R&D Systems AF2769) and CHRM3(1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were then blocked with 10% normal goat serum and incubated with primary antibodies against Iba1 (1:500, Wako Chemicals, USA) and IL-1β (1:1000, R&D Systems, USA) overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: pH 6.0 Tris-EDTA buffer was used as the antigen-retrieval buffer for the following antibodies: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Developmental Biology 2023Quote: pH 9.0 Tris-EDTA buffer was used as the antigen-retrieval buffer for the following antibodies: CD31 (R&D systems; AF3628; 1:500), SOX9 (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and incubated with fluorescence-conjugated antibodies for 15 minutes on ice: PB2 PE (phycoerythrin) (human, R&D Systems #FAB53291P), PB2 APC (human ...
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bioRxiv - Cell Biology 2023Quote: ... All sections were incubated with a biotinylated secondary antibody and stained using the R&D HRP-DAB staining kit (R&D Systems, USA) and then counterstained with hematoxylin ...
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bioRxiv - Cell Biology 2023Quote: ... and tissue harvest as previously described and in Supplementary Methods.6 Structural analysis of lung tissue included hematoxylin and eosin stain as well as immunostain for muscularization of vessels as previously described.7 Cytokine levels were measured by pooling plasma and lung lysate from 5 mice treated with either L-NAME/HFD or control water/diet for 5 weeks and was assayed using an antibody-based cytokine array (R&D Systems, ARY006) and was completed according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... slides were probed with primary antibodies (CD31 1:200, Biolegend, MEC13.3; mSPARCL1 1:500, R&D systems, #AF2836-SP; hSPARCL1 1:500, R&D systems, #AF2728-SP ...
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bioRxiv - Cancer Biology 2023Quote: ... Lymphatics were visualized by injecting 0.4ug of Mouse LYVE-1 Alexa Fluor® 594-conjugated Antibody (20ul of concentration 20ug/ml, diluted 1:10 in normal saline; cat# FAB2125T; R&D systems) into the tongue in a region near the tumor 20 minutes before imaging ...
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bioRxiv - Cell Biology 2023Quote: ... plasma and tissues of interest were collected at different post-infection times and assessed by ELISA using commercially available antibodies and according to the procedures supplied by the manufacturer (R&D Systems, Minneapolis). All samples were stored at -20°C until processing ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained 1h at RT with a goat polyclonal anti-human ACE2 (AF933, R&D Systems) diluted at 3μg/ml in PBS + 0.3% triton X-100 + 1% horse serum + 1% BSA + 0,01% sodium azide ...
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bioRxiv - Neuroscience 2020Quote: ... 4) incubation with a donkey anti-rabbit IgG conjugated with NorthernLights™ NL557 (#NL004, R&D System) diluted 1:1000 for 2 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The transduced cells were stained with anti-human ACE-2 polyclonal goat IgG (AF933, R&D Systems) primary antibody at 1 ug/mL and donkey anti-goat IgG conjugated to Alexa Fluor 488 (ab150129 ...
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bioRxiv - Bioengineering 2022Quote: ... Alexa Fluor® 647-conjugated anti-human lymphatic vessel endothelial receptor-1 (LYVE-1 ,R&D Systems) or Alexa Fluor™ 488-conjugated anti-human laminin (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: ... stimulated B cells were incubated with 20μg/ml anti–DC-SIGN mAb (clone 120507, R&D system) or mouse IgG (R & D Systems ...
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bioRxiv - Neuroscience 2019Quote: ... goat anti-platelet derived growth factor receptor-α (PDGFRα, 1:200, AF1062. R&D Systems, MN, USA), goat anti-Iba1 (1:200 ...
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bioRxiv - Pathology 2020Quote: ... goat polyclonal anti YM1/Chitinase-3 like 3 (R&D systems, Cat. No. AF2446, 1:400 dilution). Antigen retrieval of HIER2 (EDTA ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were sorted for LRP6 knockdown using flow cytometry (anti-human LRP6-APC, R&D Systems MAB1505) and successful knockdown was confirmed by Western blot.
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bioRxiv - Physiology 2021Quote: ... Human MPCs were trypsinized and incubated 30 min with biotinylated anti-human PDGFRα (BAF322 R&D Systems) goat polyclonal antibody and CD56-PE (clone B159 ...
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bioRxiv - Microbiology 2022Quote: ... The anti-DC-SIGN phycoerythrin-conjugated fragment antigen binding (Fab) 1621P was purchased from R&D Systems. Soluble C6-GlcCer was purchased from Cayman Chemical and dissolved in methanol ...
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bioRxiv - Genomics 2023Quote: ... Precleared lysates were incubated with 1 mg goat anti-EPHB4 polyclonal IgG (R&D systems, AF446-SP) at 4°C for 3 hours followed by addition of 10 ml Protein A Dynabeads (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Anti-TRAIL ELISA was performed using a Human TRAIL/TNFSF10 Quantikine ELISA Kit (R&D Systems, DTRL00) following manufacturer-provided protocols ...
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bioRxiv - Pathology 2021Quote: Formalin-fixed and paraffin-embedded liver sections were subjected to a standard procedure of immunohistochemistry with primary antibodies against F4/80 (eBioscience, San Diego, CA) and myeloperoxidase (MPO, R&D System, Minneapolis, MN). The liver sections were additionally subjected to Masson’s trichrome staining ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Human ACE2 Biotinylated Antibody (Cat # BAF933) and Recombinant Spike S1 RBD protein (Cat # NBP2-90982) were obtained from R&D Systems (Minneapolis, MN, 55413) and NOVUS Biologicals (Centennial ...
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bioRxiv - Cell Biology 2020Quote: Conditioned media of papillary and reticular fibroblasts from the three donors were analysed using human angiogenesis antibody array (R&D systems, Minneapolis MN, USA). Images were acquired using Fusion Capt Advance FX7 (Vilber ...
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bioRxiv - Cancer Biology 2021Quote: ... Then membranes were blocked in 5% non-fat milk in TBS-T for 30 minutes at room temperature and incubated with primary antibodies against Robo1 (R&D Systems, 1:500), Robo2 (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems, MAB415-100) or rat IgG antibodies (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... Immunostaining nanoparticle tracking analysis (NTA) was performed using fluorescently labeled antibody conjugate directed against human CD63 (AlexaFluor488-conjugated clone 460305; R&D Systems (Minneapolis, USA)) ...
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bioRxiv - Immunology 2021Quote: Multiplexed ELISA on interstitial fluid from matched T and NAT fresh tissues were performed on a Luminex 200 platform (Luminex Inc.,) using custom kits of pre-mixed antibody-coated beads (R&D System Inc., MN), which included the following analytes ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates and corresponding supernatants were analyzed on the same reducing SDS polyacrylamide gel and detected by Western blot analysis by using goat-α-Shh antibodies (R&D Systems, AF464), rabbit-α-GAPDH antibodies (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: Macrophage supernatants were thawed on ice and assayed for the presence of 40 cytokines using Proteome Profiler Antibody Arrays (R&D Systems, Minneapolis, MN). Procedures were followed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The cell lysates (20 μg) were electrophoresis using SDS gels and transferred to nitrocellulose membranes and the incubated with TrkB antibody (1:1000, R&D systems, goat, AF1494), CRHR1 antibody (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation with the primary antibody for platelet-derived growth factor receptor alpha (PDGFRa) (ref. AF1062-SP, R&D systems, 1/200), OLIG2 (ref ...
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bioRxiv - Immunology 2021Quote: ... PE- or APC-conjugated anti-NKG2C (Cat# FAB138P, RRID:AB_2132983 or Cat# FAB138A, RRID:AB_416838 – generously provided by R&D Systems), and then stained for intracellular expression with FITC anti-FcRγ (Millipore Cat# FCABS400F ...
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bioRxiv - Developmental Biology 2021Quote: ... Goat anti-Dll4 (*with antigen retrieval 1:50 and without antigen retrieval 1:25, AF1389, R&D Systems), *Rabbit anti-NICD activated (1:75 ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were incubated with monoclonal anti-neuron-specific beta-III tubulin (Tuj-1, MAB1195 R&D Systems), rabbit polyclonal astrocyte-specific anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD200 was pre-incubated with a 1.5-fold molar amount of human CD200Fc (R&D Systems) prior to staining ...
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bioRxiv - Microbiology 2021Quote: ... cells were blocked with 1% BSA and stained overnight with goat anti-ACE2 (1:200, R&D Systems) in blocking buffer followed by rabbit anti-goat Alexa Fluor 488 (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... 90 μl of cells were incubated with 10 μl of anti-FAP-PE (clone 427819, R&D Systems) for 20 minutes at room temperature before washing with FACS buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Pericytes were labeled by combining PDGFRβ (Goat anti-PDGFRβ, R&D Systems, cat. no.: AF1042, dilution: 1:100) and Mouse Aminopeptidase N/CD13 (Goat anti-CD13 ...
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bioRxiv - Immunology 2023Quote: ... IL-2 complex was made with 1.5μg of recombinant human IL-2 (NIH) and 7.5μg of anti-human IL-2 (R&D Systems) per injection in 100μL of sterile PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed extensively prior to incubation with goat anti-rabbit IgG (Ref# HAF008, R&D SYSTEMS, USA) HRP conjugated secondary antibody ...
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bioRxiv - Developmental Biology 2024Quote: ... were washed in 1X PBS then incubated in goat anti-GAS1 (2 mg, R&D Systems; Cat#AF2644) antibody diluted in 1X PBS for 2 h at room temperature on a rotating platform ...
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bioRxiv - Neuroscience 2020Quote: The primary antibodies used were Collagen IV (1:400 dilution; Cosmo Bio, Catalog Number LSL-LB1403) and CD13 (1:100 dilution; R&D Systems, Catalog Number AF2335). Mice were sacrificed by decapitation ...