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Citations for R&D Systems :
3951 - 4000 of 4326 citations for Cyclic AMP Direct ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed and incubated with an anti-mouse HRP-conjugated secondary antibody (R&D Systems; 1:1000) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... Anti-Human TIM-1 monoclonal antibody (MAB1750) and polyclonal antibody (AF1750) were purchased from R&D systems (Minnesota, USA). Anti-GAPDH polyclonal antibody (sc-25778 ...
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bioRxiv - Neuroscience 2022Quote: ... Then 1 ml of fresh media was supplemented with IL-34 (R&D systems, cat. No. 5265-IL-010), MCSF (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The neural medium was supplemented with epidermal growth factor (EGF, 20 ng ml-1, R&D Systems, 236-EG) and basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2024Quote: ... and anti-B220 (Rat anti-mouse; monoclonal: RA3-6B2 clone; dilution 1:100 in blocking solution; R&D systems). Secondary antibody staining was performed for 50min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Human vasculature labeling was performed by using a cocktail of goat anti-podocalyxin (1:20, AF1658, R&D Systems), mouse anti-CD31 (1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... coverslips were washed in PBS and coated with 1 µg / mL laminin-I (R&D Systems; #3446-005-01) at 37 °C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were pelleted and then resuspended in 1 mL of Flow Cytometry Staining Buffer 1X (R&D Systems #FC001) for blocking ...
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bioRxiv - Cancer Biology 2023Quote: ... in 2% BSA/PBS solution and the appropriate fluorescently-conjugated secondary antibody (R&D Systems catalog #NL006, 1:200). Coverslips were mounted onto slide with DAPI-containing ProLong Gold media ...
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bioRxiv - Bioengineering 2023Quote: ... The primary antibodies used in this study include anti-Mouse Myosin Heavy Chain (1:1000, R&D Systems, MAB4470), anti-Human/Mouse/Rat/Chicken Pax7 (5μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: Western Blotting primary antibodies and working dilutions used were: goat anti-CD276 (1 µg/ml; R&D systems, #AF1027); mouse anti-α-tubulin (1:1’000 ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used at the indicated dilutions: goat anti-Nurr1 (AF2156, R&D Systems; 1:250), rabbit anti-Nr2f2 (ab211776 ...
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bioRxiv - Neuroscience 2023Quote: ... Specified primary antibodies were incubated over-night at 4°C: goat anti-ICAM1 (1:400, R&D systems AF796), rat anti-cluster differentiation 31 (CD31 ...
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bioRxiv - Neuroscience 2023Quote: ... The neural medium was supplemented with 20 ng ml−1 epidermal growth factor (EGF; R&D Systems, 236-EG) and 20 ng ml−1 basic fibroblast growth factor (FGF2 ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used for immunostaining of mice tissues: goat anti-CD31 (1:500; R&D systems), goat anti-LYVE1 (1:1,000 ...
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bioRxiv - Physiology 2024Quote: ... PDGFR-α (goat anti human, 1:300, cat. no.: AF-307-NA; RRID: AB_354459, R&D Systems, Bio-Techne), and C3 (rabbit anti human ...
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with 1 ng/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... followed by matching horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature (R&D Systems, MN, USA). Chemiluminescent imaging was performed using the LAS-4000 platform and histogram densitometry was performed using Multi Gauge software (V3.1 Fujifilm ...
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bioRxiv - Neuroscience 2020Quote: ... Cytokine release was assessed using magnetic multiplex assay (Rat Magnetic Luminex® Assay, Rat Premixed Multi-Analyte Kit, R&D Systems, Bio-Techne, R&D Systems Inc., USA) on a Bio-Plex 200 station (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2019Quote: Serum concentrations of tumor necrosis factor-α (TNF-α) and interleukin1β (IL-1β) were measured on 8th week using Valukine™ immunoassay kits (R&D Systems, Inc., Minneapolis, USA) according to the manufacturer’s instructions using microplate reader (Synergy 2 ...
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bioRxiv - Systems Biology 2020Quote: ... Primary detection of hACE-2 was done using a goat anti-hACE-2 antibody (1:1,250; #AF933, R&D Systems), a horseradish peroxidase (HRP)-labeled donkey anti-goat antibody (1:5,000 ...
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bioRxiv - Immunology 2021Quote: ... Slides were then incubated in permeabilization buffer containing the primary REG3β antibody (1:250 dilution; R&D systems, Minneapolis, MN) overnight (16 h) ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies for western blot assays were used at the following concentrations: anti-SP-D (1:2,000, R&D Systems, AF1920), anti-LPO (1:2000 ...
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bioRxiv - Developmental Biology 2020Quote: ... At day 1 the medium was changed to base media supplemented with Activin A (100 ng/ml, R&D Systems), basic FGF (bFGF ...
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bioRxiv - Immunology 2022Quote: ... PE-conjugated anti-CTLA-4 and APC-conjugated anti-CD279 (PD-1) were purchased from R&D System (Minneapolis, MN). Human cytokines from cell culture supernatants were analyzed using ELISA kits for TNF ...
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bioRxiv - Immunology 2019Quote: ... (IRF4:rat monoclonal, eBiosciences, UK, mouse monoclonal: IRF8, eBiosciences, BATF, R&D Systems, JUN Millimark, UK, PU.1 Biolegend, UK). IRF1 staining was done using rabbit polyclonal anti-human IRF1 antibody (Abcam ...
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bioRxiv - Bioengineering 2019Quote: ... we mixed laminin at 50 µg/mL with 50 µg/mL recombinant nidogen-1 (R&D systems 2570-Nd-050). Samples were immunostained as described below.
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight with a mixture of primary goat anti-ACE2 at 1/500 dilution (R&D Systems, #AF3437) and rabbit anti-GnRH (gonadotropin releasing hormone ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Physiology 2020Quote: ... Postprandial plasma obtained at termination of studies (see above for details) was used to measure IGF-1 (R&D Systems), Activin-A (R&D Systems) ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1 ...
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bioRxiv - Cell Biology 2020Quote: ... Afterwards cells were incubated with primary antibody against PDGFRa (goat, 1:200, AF-307-NA, R&D Systems, MN, USA), CD90 (mouse ...
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bioRxiv - Cell Biology 2020Quote: ... For proximity ligation assays VE-cadherin was detected using mouse VE-cadherin antibody (1:200, R&D systems, Goat, AF1002). c-Src was detected using anti-Src (GD11 clone ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg/ml CD40-ligand-HA with 5 ng/ml anti-HA antibodies (both R&D Systems, Minneapolis, USA), and Type B CpG OND D-SL01 (InvivoGen ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant VCAM-1/human Fc chimera protein was purchased from R&D Systems (R&D Systems Cat# 862-VC-100).
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bioRxiv - Cancer Biology 2020Quote: ... 8 μm cryosections were fixed in acetone and incubated with goat-anti-mouse CD31 (R&D system, #AF3628, 1:200) and rabbit-anti-FITC (Biorad ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibody information is as following: Goat anti-mouse aminopeptidase N/ANPEP (CD13; R&D systems; AF2335; 1:100), Rat anti-mouse vascular adhesion molecule (VCAM1 ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus ...
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bioRxiv - Microbiology 2021Quote: ... were immunoblotted for ACE2 and β-actin using primary antibodies (1:500 polyclonal goat anti-human ACE2, R&D Systems, AF933 ...
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bioRxiv - Microbiology 2021Quote: ... 10ng/ml interleukin 4 (IL-4) and 10ng/ml transforming growth factor beta 1 (TGFβ1) (R&D systems, Minneapolis, MN), and used between days 7-9 of differentiation.
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bioRxiv - Cancer Biology 2021Quote: ... or 1h at 37°C with the sheep polyclonal anti-TWIST2 (1/200, AF6249, R&D Systems, Minneapolis, MN, USA) in Antibody Diluent Buffer from Ventana Medical Systems ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies and dilutions used for immunohistochemistry in this study were the following: PDGFRA (goat, 1:70; R&D Systems); GPNMB (goat ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4 and then incubated overnight at 4°C with mouse monoclonal anti-CD154 (1:50, R&D systems, MAB617) and rabbit anti-calbindinD28k (1:500 ...
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bioRxiv - Molecular Biology 2019Quote: 10 μl in vitro ubiquitylation assay reactions in 1 x ubiquitin conjugation reaction buffer (R&D systems Cat. # B-70) contains 10 mM Mg2+-ATP solution pH 7.0 (R&D systems Cat ...
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bioRxiv - Molecular Biology 2019Quote: 10 μl in vitro SUMOylation assay reaction in 1 x SUMO conjugation reaction buffer (R&D systems Cat. # B-60) contains 10 mM Mg2+-ATP solution pH 7.0 (R&D systems Cat ...
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bioRxiv - Physiology 2020Quote: ... followed by overnight incubation at 4 °C with primary antibodies against UCP1 (1:1000, R&D systems, Minneapolis, MN, USA), 14-3-3ζ (1:1000 Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were applied overnight at 4 °C: goat anti-OTX2 (1:500, R&D Systems, cat# AF1979), mouse anti-CDX2 (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were visualized by incubation with HRP-conjugated IgG Secondary antibody (anti-goat, 1:5000; # HAF017, R&D Systems, goat anti-rabbit ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... then resuspended in 20 μl of 1× ubiquitin reaction buffer containing 200 nM UBE1 E1 (R&D systems, U-110), 1 μM UbcH5a E2 (Enzo ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-β1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...