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Citations for R&D Systems :
351 - 400 of 406 citations for n4 Benzoyl 5 methyldeoxycytidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 3 independent vials of iCell microglia were seeded at 5 x 104 cells per well across 3 independent 96-well plates and treated the following day with vehicle or 5 ng/mL human IFN-β (R&D Systems). Cells were treated for 6 or 24 h prior to collection and stored at −80°C prior to RNA extraction.
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in a humidified incubator at 37∘C and 5% CO2 and tested mycoplasma negative (MycoProbe® Mycoplasma Detection Kit, R&D systems). HEK293T cells ...
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bioRxiv - Molecular Biology 2024Quote: ... supernatant was collected and centrifuged (3000 rpm, 5 min, RT) and Secreted IL6 was measured using the Quantikine® ELISA kit (R&D Systems), following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... as previously described.30 The concentrations of function blocking antibodies used were: 1 μg mL−1 Anti-laminin 5 antibody (R&D systems, #MAB2144), 1 μg mL−1 α3 anti-integrin antibody ...
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bioRxiv - Immunology 2022Quote: Substrate functionalization was performed by overnight incubation with 5 µg/ml CCL21 and 50 µg/ml ICAM1 (R&D Systems, Minneapolis, MN, USA), in PBS.
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bioRxiv - Neuroscience 2023Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology, sc-2025; normal rabbit IgG, R&D Systems, AB-105-C ...
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bioRxiv - Cell Biology 2023Quote: ... and tissue harvest as previously described and in Supplementary Methods.6 Structural analysis of lung tissue included hematoxylin and eosin stain as well as immunostain for muscularization of vessels as previously described.7 Cytokine levels were measured by pooling plasma and lung lysate from 5 mice treated with either L-NAME/HFD or control water/diet for 5 weeks and was assayed using an antibody-based cytokine array (R&D Systems, ARY006) and was completed according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... HUVECs were seeded in tissue culture-treated 12-well plates coated with human fibronectin (5 µg/cm2) (R&D Systems, Cat. # 1918-FN). Stimulated and unstimulated groups were formed as described above ...
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bioRxiv - Cell Biology 2024Quote: ... 400 µL of a 5 µg mL-1 biotinylated polyclonal Goat anti-Vascular Endothelial Growth Factor IgG solution in PBS (R&D Systems, USA) was added to CellStudio substrates containing streptavidin coated microbeads patterns (500 nm diameter beads ...
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bioRxiv - Cancer Biology 2024Quote: ... and 5% fetal Bovine serum (Hyclone, #SH300084) and treatment with 2.5 ng/ml of recombinant Human TGF-β 1 Protein (R&D systems, #RDS240B002) for maximum of 18 days ...
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bioRxiv - Cell Biology 2024Quote: ... for 30 min on a rotator at 4 °C and then transferred to a new tube with 5 μl TSC1 antibody (R&D Systems, MAB4379) or 25 μl mouse IgG Control (SC-2025 ...
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bioRxiv - Microbiology 2022Quote: ... Blots were incubated for 1 hour with HRP-conjugated secondary antibodies diluted in 5% skim milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were administered either a 5 μL volume of phosphate-buffered saline (PBS) containing 10 μg/kg of carrier-free rmIL-1β (R&D Systems, Minneapolis, MN) or PBS alone (control ...
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bioRxiv - Cell Biology 2021Quote: ... Thy1+ Rep-MEFs were cultured in ‘iMPC medium’ containing 2 μg/mL of dox and administrated three small molecules: 5 μM of Forskolin (F) (R&D Systems, 1099/50), 5 μM of RepSox (R ...
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bioRxiv - Microbiology 2021Quote: ... The medium for differentiation consisted of DMEM/F12 supplemented with 5% R-Spondin1 conditioned medium (home made from Cultrex 293T R-spondin1-expressing cells, R&D Systems, MN, USA), 10% Noggin conditioned medium (home made with HEK293-mNoggin-Fc cells ...
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bioRxiv - Cell Biology 2021Quote: ... media was replaced with RPMI supplemented with B27 without insulin and containing 1 µM CHIR99021 and 5 ng/ml BMP4 (R&D systems #314-BP). After an additional 48 hours (Day 3) ...
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bioRxiv - Cell Biology 2020Quote: ... were covered with HUVECs and embedded 24 hours later in a 2,5 mg/mL fibrin gel in the presence or absence of VEGFA-165 at 5 ng/mL (R&D systems, Minneapolis MN, USA). Conditioned media from papillary or reticular fibroblasts were added on top of the gel and renewed every day for four days ...
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bioRxiv - Pathology 2022Quote: ... blocking-and antibody-incubations were performed in TBS + 0,1% Tween20 + 5% milk. Following primary antibodies (Suppl. Table 1) were used: anti-NRP1 (1:1000, R&D Systems, Cat. No. AF566), anti-NRP2 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Filters were blocked in 5% nonfat milk in TBS-Tween 20 (0.1%) and incubated overnight with rabbit anti-NrCAM (R&D Systems AF8538, 1:1000), mouse anti-Rac1 (BD Biosciences # 610651 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with PBS and stimulated with cytokine cocktail consisting of IL-1β (5 ng/ml, R&D Systems, Minneapolis, MN, USA), and IFN-γ (50 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems, MAB415-100) or rat IgG antibodies (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: ... were cultured for 7 days in RPMI supplemented with 5% decomplemented autologous human blood plasma and 10 ng/mL M-CSF (R&D system, #216-MC) to obtain MDMs ...
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bioRxiv - Cell Biology 2022Quote: ... MDA-MB-231 and HEK293T cells (ATCC) were cultured in DMEM (4.5 g/liter glucose, pH 7.2) with 5% FBS (Atlanta Biologicals/R&D Systems #S11150, Lot #F18071) 20 mM Hepes ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 5 hours in DermaLife Basal Medium followed by stimulation with human recombinant IL-17A (R&D systems, 50 ng/ml) and TNF-α (R&D systems ...
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bioRxiv - Physiology 2023Quote: ... 1nM) in VEH for 60 minutes prior to the addition of recombinant human TGF-β1 (5 ng/ml; R&D Systems, Minneapolis, MN, USA). At 6 ...
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bioRxiv - Genetics 2023Quote: ... with the addition of the TET inhibitor molecule Bobcat 339 (concentration of 5 µM, 10 µM or 30 µM, R&D Systems, 6977/10) or in the absence of ascorbic acid (normally added to the reprogramming medium at 25 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Then lysates were utilized exactly according to the instructions in the Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA Kit (R&D systems, DYC2724-5).
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bioRxiv - Physiology 2022Quote: ... and were further stratified to either treatment of 5 d/wk injections of recombinant sclerostin (0.1 mg/kg body mass) (cat# 1589-ST; R&D Systems, Minneapolis, Minnesota, USA) or saline control ...
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bioRxiv - Neuroscience 2022Quote: ... a mix of 10 μg/mL Laminin and 5 μg/mL of each SLRP protein (recombinant human CHAD (R&D Systems Cat#8218-CH), FMOD (R&D Systems Cat#9840-FM) ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Bioengineering 2024Quote: ... foregut cells in the 24-well plate or on the pillar plate were cultured in advanced DMEM/F12 supplemented with 5 ng/mL recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... at 37°C in 5% CO2 in a humidified incubator in IMDM with phenol red supplemented with 10% fetal bovine serum (FBS) (R&D systems, Minneapolis, MN), 1mM Sodium Pyruvate ...
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bioRxiv - Neuroscience 2023Quote: ... After transfection neurons were washed twice with NBP and treated with 20 μM (2R)-amino-5-phosphonovaleric acid (AP5; R&D Systems, 0105/10), an NMDA receptor antagonist to prevent transfection-induced neuronal overexcitation ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration was measured with BCA assay and 350 µg of lysates were incubated with 5 μg of anti-Siglec-E antibody (polyclonal goat IgG - R&D Systems – cat. AF5806) or goat IgG for 2h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... and from Days 2 to 5 CHIR99021 was withdrawn and replaced with 100 ng/mL Activin A (R&D Systems, 338-AC-050/CF). Media change was performed every day by discarding spent media ...
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bioRxiv - Cell Biology 2024Quote: ... This medium is based on DMEM/F12 supplemented with 5% R-Spondin1-conditioned medium (made from Cultrex 293T R-spondin1-expressing cells; R&D Systems, MN, USA), 10% Noggin-conditioned medium (made with HEK293-mNoggin-Fc cells ...
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bioRxiv - Immunology 2024Quote: ... BAL was collected and pooled from groups of 5 mice and cultured for 6 hours at 370 with 5% CO2 in the presence of mouse recombinant IL-4 (R&D systems, 404-ML) and IL-10 (R&D Systems 417-ML ...
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bioRxiv - Immunology 2024Quote: ... and the adherent irradiated 40LB cells were co-cultured with 5 × 104 naïve splenic B cells in 6 mL of B cell media containing IL-4 (R&D Systems, 1 ng/mL) for 96 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cytokines released from HCEC-1CT cells into cell culture supernatants after 5 h of incubation were measured using a Magnetic Luminex® assay (R&D SYSTEMS, Minneapolis, USA). Cell culture supernatant samples were collected after the incubation for cytokine gene transcription (details for incubation see 2.4. ...
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bioRxiv - Microbiology 2020Quote: ... Primary BMDCs were isolated from C57BL/6 mice and cultured in Dulbecco’s modified Eagle medium for 3–5 days in the presence of 20 ng/mL recombinant granulocyte-macrophage colony-stimulating factor (R&D Systems, Minneapolis, MN, USA) as described previously (83) ...
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bioRxiv - Genomics 2022Quote: ... samples were thawed and cleared by centrifugation for 5 minutes at 1000xg and assayed for urine MMP7 and MMP9 by ELISA according to manufacturer’s instructions (R&D Systems, DMP700/DMP900, Minneapolis, MN). Urine creatinine was used to normalize MMP7 and MMP9 levels between non-active ...
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bioRxiv - Cancer Biology 2020Quote: ... Invasion was also quantified in response to serum-free DMEM supplemented with 10 ng/mL of recombinant mouse CSF2 (R&D Systems, 415-ML-5), PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems ...
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bioRxiv - Molecular Biology 2020Quote: Hyaluronic acid of COVID-19 patients’ plasma was measured in 1:5 dilution by the enzyme-linked sandwich assay Hyaluronan DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s descriptions ...
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bioRxiv - Microbiology 2021Quote: ... Memory CD4+ T-cells were infected with HIV-1 (50 ng HIV-p24/106 cells) and then cultured in the presence of IL-2 (5 ng/ml; R&D Systems, Minneapolis, MN, USA) for up to 9 days ...
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bioRxiv - Immunology 2020Quote: ... For iTreg cell differentiation we additionally added the following cytokines and blocking antibodies: rmIL-2 and rmTGF-β (both: R&D Systems, 5 ng/ml), anti-IL-4 (clone ...
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bioRxiv - Genetics 2023Quote: Reprogramming was induced in the presence or absence of IFNγ at 10 ng/ml (from day 0 to 5) (R&D Systems, 485-MI-100). SSEA1+ day 5 iPSCs (4 replicates from different reprogramming rounds ...
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bioRxiv - Molecular Biology 2023Quote: MCF7 and T47D cell lines were perturbed using 5 ng/mL of recombinant human TGF-β (R&D Systems 240-B-002/CF, Minneapolis, MN) over the course of three days ...
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bioRxiv - Bioengineering 2023Quote: ... Human mesenchymal stem cells (Passage 5-7) (commercially obtained from Rooster Bio (Frederick, MD)) were cultured in hMSC growth media (R&D Systems, Minneapolis, MN, USA). Adipose-derived stem cells (Passage 3-5 ...
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bioRxiv - Cancer Biology 2024Quote: ... spheres incubated under non adherent conditions for 5 days were challenged with the addition of 500 ng/ml human recombinant Netrin-1 (R&D Systems #6419-N1-025) for a period of 20 minutes before collection ...
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bioRxiv - Microbiology 2024Quote: ... cecal samples were diluted 1:5 in reagent diluent and quantified by ELISA using the DuoSet Mouse S100A8/S100A9 Heterodimer kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s recommendations ...