Labshake search
Citations for R&D Systems :
351 - 400 of 2812 citations for IL 2RA Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... at 37°C in RPMI medium supplemented with 8% human serum and cultured for 7-9 days in the presence of 50 UI/ml of recombinant IL-2 (R&D System).
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bioRxiv - Immunology 2023Quote: ... 600,000 cells plus culture medium were replaced in a 24 well plate containing a 50-60% confluent monolayer of unstimulated ADSCs with or without addition of anti-human IL-1β (2ug/mL, R&D Systems) or a mouse IgG1 isotype control (2ug/mL ...
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bioRxiv - Immunology 2023Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
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bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
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bioRxiv - Cancer Biology 2022Quote: ... miniloci in an endogenous IgH and Igκ knockout background (19,20) were immunized with recombinant human LAG-3–Fc protein (hLAG-3–hFc) (R&D Systems, Minneapolis, MN, USA), consisting of the extracellular domain of LAG-3 (Leu23-Leu450 ...
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bioRxiv - Cancer Biology 2022Quote: ... +/-1 µg/mL anti-CD28 (CD28.2) antibody (eBioscience/Thermo 16-0289-81) or recombinant human CD58-Fc chimera protein (R&D Systems 10068-CD-050) at 37 ºC for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 1 μg/mL of either human rPD-1 (hrPD-1-Fc; R&D Systems #1086-PD-050; Minneapolis, MN, USA) or mouse rPD-1-Fc (R&D Systems #1021-PD-100 ...
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bioRxiv - Bioengineering 2024Quote: ... 0.1% v/v FBS with or without 100 ng/ml Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN). Medium change was performed at day 3 and images were taken at 3 and 5 days using a Keyence BZ-X800 Fluorescence Microscope (Keyence ...
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bioRxiv - Bioengineering 2024Quote: A total of 1 μg Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) was dissolved in GelMA/fibrin hydrogel containing thrombin and transferred into a cylinder-shaped mold with a liquid capacity of 8.6 μl and 1 mm diameter with 1 mm thickness ...
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bioRxiv - Bioengineering 2024Quote: ... Different concentrations of endogenous Cyr61 (0-200 ng/ml; Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) and TGFβ (0 or 10 ng/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... or ULBP4-Fc (R&D Systems) coated onto microtiter plates ...
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bioRxiv - Immunology 2020Quote: ... mouse ICAM1-Fc (R&D systems), FxCycle Violet Stain ...
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bioRxiv - Cancer Biology 2023Quote: ... EphrinB1-Fc (R&D systems, USA) and control Fc fragment (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... IL-6 and IL-10 in media (DuoSet, R&D Systems Minneapolis, USA). ELISA procedures were carried out according to manufacturer protocols.
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bioRxiv - Biophysics 2022Quote: ... and rmIL-33 (402-ML, 1399-IL, and 3626-IL, R&D Systems). Stimuli for human ILC2s were recombinant human IL-2 (rhIL-2 ...
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bioRxiv - Immunology 2020Quote: ... IL-13 (rIL-13; Prepotech) or IL-25 (rIL-25; R&D Systems) (concentration ...
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bioRxiv - Immunology 2022Quote: ... IL-33 concentrations were measured by ELISA (IL-33 duoset, R&D systems) and detected using horseradish peroxidase-conjugated streptavidin and TMB substrate (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... IL-10 and IL-22 (DuoSet ELISA R&D Systems, Minneapolis, MN, USA) IFN-γ (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Tissue culture 96-well plates were coated with DL4-Fc and VCAM-1-Fc (R&D Systems) overnight at 4°C or for 2-4 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Recombinant Fc receptors (FcγRIIIa/CD16a, FcγRIIa/CD32a H167, FcγRIIb/CD32b, Neonatal Fc receptor/FcRN) (R&D Systems) were labeled with PE (Abcam ...
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bioRxiv - Microbiology 2021Quote: IL-1β and IL-6 were detected in tissue lysate using the Mouse IL-1β and IL-6 DuoSet ELISA kits (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the media were refreshed and supplemented with 5 ng/mL IL-7 or 5 ng/mL IL-7 and 4 ng/ml IL-2 (R&D Systems), respectively ...
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bioRxiv - Immunology 2020Quote: IL-27(p28) in BALF was measured with a mouse IL-27 p28/IL-30 DuoSet ELISA (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... supernatants were collected and the released IL-1β were measured by mouse IL-1β/IL-1F2 DuoSet ELISA (Cat. number: DY401) from R&D Systems.
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bioRxiv - Microbiology 2023Quote: The IL-1β levels in supernatant samples were measured using the Mouse IL-1β/IL-1F2 ELISA kit (R&D Systems, DY401), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... ELISAs were performed to quantify IL-1β in these culture supernatants using the Mouse IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems) per the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested using the Quantikine human IL-6 (sensitivity < 5 pg/mL) ELISA kit (R&D Systems, Inc., Minneapolis, MN) according to manufacturer’s recommended conditions ...
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bioRxiv - Microbiology 2019Quote: ... IL-8 production was also determined by analysis of basolateral supernatants from VDC’s using a human IL-8 ELISA kit (R&D systems, Minneapolis, USA) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... Plasma IL-6 was assessed in duplicate using high-sensitivity enzyme-linked immunosorbent assay (ELISA; Quantikine HS Human IL-6 ELISA, R&D Systems, UK) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... PBMCs at a cell density of 1×106 cells/ml were stimulated with a mixture of 25 ng/ml human IL-10 (R&D Systems, UK), 0.5 µg/ml of pokeweed mitogen (PWM) ...
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bioRxiv - Genomics 2019Quote: ... as from previous dose-response experiments3–5: recombinant human IL-1β (specific activity 1.8×107 U/mg; 201-LB-005, R&D Systems, Abingdon, UK) at 50 U/ml ...
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bioRxiv - Immunology 2020Quote: ... Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems; R15-100). R15-100 was added every 2 to 3 days thereafter ...
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bioRxiv - Immunology 2022Quote: Detection of secreted IL11 into the supernatant of HPAEpiC cultures was performed using the human IL-11 ELISA kit (R&D systems, D1100) according to manufacturers’ instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and incubated with recombinant SARS-CoV-2 Spike protein receptor binding domain (RBD) fused to the human IgG1 Fc domain (R&D Systems 10499-CV-100) or control recombinant human IgG1 Fc protein (R&D Systems 10499-CV-100 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Concentrations of IL-6 and IL-1β were measured using the Quantikine Mouse IL-6 ELISA Kit (R&D Systems, Minneapolis, MN, USA) and Quantikine Mouse IL-1β ELISA Kit (R&D Systems) ...
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bioRxiv - Neuroscience 2022Quote: IL-1β in microglia culture supernatant was measured with Mouse IL-1β /IL-1F2 DuoSet ELISA Kit (R&D Systems DY401, Minneapolis MN) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 5 ng mL-1 of IL-7 and IL-15 (R&D Systems). Regulatory T cells were activated at 1 x 106 cells mL-1 for 2 days in complete XVivo15 supplemented with magnetic Treg Xpander CTS Dynabeads (ThermoFisher ...
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bioRxiv - Immunology 2019Quote: ... 5 ng/ml IL-7 and 5 ng/ml IL-15 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... interleukin 6 (IL-6) and IL-1β were quantified by ELISA (R&D Systems) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... IL-1β and IL-6 were detected by ELISA duo sets (R&D Systems) following the manual ...
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bioRxiv - Immunology 2020Quote: ... IL-13 and IL-12 p70 ELISA kits were purchased from R&D systems, IFNγ and IL-10 from BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... IL-1β protein was measured using IL-1β (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... 1 ng/ml IL-12 and 5 ng/ml IL-15 (R&D Systems). Re-stimulation with irradiated (100 Gy) ...
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bioRxiv - Immunology 2023Quote: ... 10 ng/ml IL-7 and 5 ng/ml IL-15 (R&D Systems). After 14 days ...
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bioRxiv - Cancer Biology 2023Quote: ... Levels of IL-8 were assessed using ELISA (R&D Systems (IL-8 #DY208). The data were normalized to cell number and reported as fold change of secreted protein compared to control.