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Citations for R&D Systems :
351 - 400 of 481 citations for 7 8 Dihydro 6 5H quinolinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and cultured for 6 days with recombinant GM-CSF (R&D Systems cat. 215-GM, 50 ng/ml) and IL-4 (R&D Systems cat ...
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bioRxiv - Cancer Biology 2023Quote: Antibodies used for FACS: Human/mouse/bovine integrin alpha 6/CD49f PE-conjugated antibody (FAB13501P, R&D Systems); PE/Cyanine 7 anti-mouse CD325 (Ep-CAM ...
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bioRxiv - Developmental Biology 2022Quote: ... day 5 lateral plate mesoderm or day 7 differentiating lung mesenchyme was transferred to cSFDM supplemented with 100 ng/ml rmVEGF (R&D Systems, # 493-MV-005). For evaluation of differentiation efficiency ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse VEGFR3 domains 1-4 and 4-7 were detected by probing with the polyclonal goat anti-mouse VEGFR3 antibody (R&D Systems, AF743, 1:1000) against the extracellular domain of VEGFR3 ...
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bioRxiv - Cell Biology 2022Quote: Proteasome activity was assessed similarly by incubating cells with genetic (7 days) or chemical (1 hour) perturbations with Me4BoVS (R&D system; I-190-050). After incubation at 37ºC for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... and Fluorogenic Peptide Substrate VII: Z-Leu-Arg-4-methyl-7-coumarylamide (Z-LR-AMC)(Catalog # ES008) were purchased from R&D Systems (Minneapolis, MN, USA). The enzyme reactions were initiated upon addition of ice-cold of hrCTSL (96pM ...
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bioRxiv - Bioengineering 2023Quote: ... Human mesenchymal stem cells (Passage 5-7) (commercially obtained from Rooster Bio (Frederick, MD)) were cultured in hMSC growth media (R&D Systems, Minneapolis, MN, USA). Adipose-derived stem cells (Passage 3-5 ...
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bioRxiv - Cell Biology 2020Quote: ... and then were seeded into upper chamber of transwell inserts (8 μM pore size, Costar) with or without coated Matrigel (R&D System). The lower chamber of the transwell was filled with 750 μl medium supplemented with 10% FBS which function as a chemoattractant ...
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bioRxiv - Cell Biology 2020Quote: ... An equal amount of protein from each sample was subjected to quantitative analysis of IL-8 and IL-1β using ELISA kit (R&D system) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Microbiology 2021Quote: Protein expression was analyzed by polyacrylamide gel electrophoresis on 8%-16% precast gradient gels (Thermo) and Western blotting using antibodies to ACE2 (AF933, R&D Systems), c-Myc-epitope (clone 9E10 ...
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bioRxiv - Cancer Biology 2019Quote: ... and the corresponding cryostat tissue sections (8-10μm thickness) were stained using the following primary antibodies: goat anti-CD31 (R&D System; AF3628), rabbit anti-caspase-3 (Sigma ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested to evaluate hIL-8 secretion by enzyme-linked immunosorbent assay (ELISA) according to manufacturer’s instructions (R&D Systems, U.S.A.). Cell protein concentrations were determined using the BCA Protein Assay kit (Thermofisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Bioengineering 2022Quote: ... The streptavidin modified nanovial solution was then mixed with a solution containing an antibody cocktail comprising 8 µg/mL anti-mouse CD45 (R&D Systems) biotinylated according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and IL11 in equal volumes of cell culture media collected from experiments with primary human cells were quantified using Human IL-8/CXCL8 Quantikine ELISA Kit (D8000C, R&D Systems), Human IL-6 Quantikine ELISA Kit (D6050 ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed EVs were concentrated with 3 kDa MWCO centrifugal filters and characterized via Luminex Multiplex Assay for TNF-α and IL-8 according to the manufacturer’s instructions (R&D Systems). Results were normalized by total protein content ...
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bioRxiv - Molecular Biology 2023Quote: ... and apurinic or apyrimidinic (AP or abasic) sites in the samples were performed using HT 8-oxo-dG ELISA Kit (R&D Systems) and AP Sites Quantitation Kit (Cell Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... It was counted as Day 0 when medium was changed to SF medium with 8 ng/mL recombinant Activin A (R&D Systems), 5 ng/mL recombinant human VEGF 165 Protein (R&D Systems ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...
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bioRxiv - Neuroscience 2024Quote: ... onto Matrigel (BD Biosciences)-coated culture plates at a ratio of 1:4∼1:8 supplemented with ROCK inhibitor Y-27632 (10 mM, R&D systems). To monitor and ensure the genetic stability of the cells ...
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bioRxiv - Cell Biology 2020Quote: ... and IL-6 (assay detection range 9.38-600 pg/ml) were purchased from R&D Systems (Minneapolis, MN, USA) and were used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The specificity of staining was confirmed by staining with rat IgG (ref:6-001-F, R&D Systems, Biotechne) or rabbit IgG (ab27478 ...
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bioRxiv - Developmental Biology 2021Quote: ... into chondroprogenitor cells (6 days; 20 ng/ml BMP4 [cat. num. 314-BP-010CF; R&D Systems, Minneapolis, MN]). Mesodermal differentiation medium had a base of Iscove’s Modified Dulbecco’s Medium ...
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bioRxiv - Neuroscience 2021Quote: ... Supernatants were collected and assayed for IL-6 and TNF-α by ELISA Kits (R&D Systems, Minneapolis, MN).
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bioRxiv - Bioengineering 2020Quote: ... supernatants were collected and IL-6 and TNF-α levels were determined using DuoSet ELISA kits (R&D Systems) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Mouse IL-6 was measured in cell supernatants using a DuoSet ELISA kit per manufacturer instructions (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... Mouse IL-6 was detected from cell culture supernatants by ELISA according to the manufacturer’s instructions (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 in cell supernatants was determined by ELISA following the manufacturer’s instructions (R&D Systems). Results were read in a Synergy Mx plate reader (BioTek).
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bioRxiv - Immunology 2022Quote: ... PPS14-TT together with recombinant IL-6 (500 ng/adult and 100 ng/neonate (R&D systems, Minneapolis, MN) was emulsified with aluminum hydroxide ...
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bioRxiv - Physiology 2022Quote: ... mouse IgG2a kappa (eBM2a) (eBioscience™, 14-4724-82) and the rat IgG (R&D Systems, 6- 001-F).
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bioRxiv - Physiology 2024Quote: ... Then 6 hours later NRVMs were treated with PBS or 100ng/mL IGF1 (291-G1-01M; R&D Systems) to elicit physiological hypertrophy for 48 hours prior to cell harvesting for experiments ...
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bioRxiv - Microbiology 2019Quote: ... IL-8 production was also determined by analysis of basolateral supernatants from VDC’s using a human IL-8 ELISA kit (R&D systems, Minneapolis, USA) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... 50μl of mitochondria (1μg/μl protein) were treated with Caspase-8-cleaved tBid from 5-500nM (882-B8-050, R&D Systems, Minneapolis, MN), Bim-BH3 from 10-10,000nM (Ac-DMRPEIWIAQELRRIGDEFNAYYARR-amide ...
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bioRxiv - Microbiology 2021Quote: ... The levels of IL-1β and IL-8 in the conditioned medium were measured by ELISA using the human IL-1β DuoSet (R&D Systems; # DY20105) and the human IL-8 set (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... IL-8 and TNF-α were quantified in the supernatants from uninfected and SARS-CoV-2-infected hiPSC-CMs by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... progranulin concentrations were determined in duplicate using 10–15 μl of lysates per well (typically 8–20 μg of total protein per well) using a sandwich ELISA assay (R&D Systems, DPGRN0). For experiments analyzing secreted progranulin ...
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bioRxiv - Immunology 2022Quote: ... and MRP-8/14 in human blood serum were measured by ELISA kits (BioLegend, R&D Systems, or Shino-Test Corp., Tokyo, Japan) following the manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... and then incubated with anti-8-oxo-dG antibody (1:400 in 1% goat serum; R&D Systems, 4354-MC-050, Lot# P275856) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was probed with an anti-EFA6R rabbit polyclonal antibody (1:500) (8) or an anti-β-actin mouse monoclonal antibody (1:10,000) (R&D Systems, UK) diluted in blocking buffer ...
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A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... IL-6 and TNF-α were measured by enzyme-linked immunosorbent assays (ELISAs) using kits purchased from R&D Systems (DY400-05 ...
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bioRxiv - Immunology 2020Quote: ... IL-10 and IL-6 were measured by sandwich ELISA using the Duo Set ELISA kit (R&D Systems, UK) (23 ...
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bioRxiv - Cell Biology 2020Quote: ... retinae from C57bl6 and Cx3cr1GFP/GFP animals (n = 6) were incubated as above with fractalkine (200ng/ml, R&D Systems) or PBS for 2 hours at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and M2d MΦs from stimulation with and 50 ng/mL recombinant human IL-6 (R&D Systems; Minneapolis, MN, USA) and 25 ng/mL recombinant human leukemia inhibitory factor (LIF ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-6 in cell culture supernatant were determined using the mouse DuoSet kit (R&D Systems, Minneapolis, MN). All assays were performed according the manufacturer’s supplied protocol ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed for RNA analysis and supernatants were analysed for IL-6 content by ELISA (DuoSet, R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... and IL-6 levels were quantified in BALF and lung tissues using commercially available DuoSet ELISA kits (R&D Systems) according to manufacturer’s instructions and published protocols 22 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells (1 × 104) were seeded on 8 µm Boyden chamber inserts (Becton Dickinson Labware, Bedford, MA)(23) coated with fibronectin (R&D Systems, Minneapolis, MN) (5 μg/ml ...