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• Overexpression of Alpha-1 Antitrypsin Increases the Proliferation of Mesenchymal Stem Cells by Upregulation of Cyclin D1 and is Independent of the Wnt Signaling Pathway

  Bryan Wolf, et al., bioRxiv - Cell Biology 2023

  Quote: ... rat anti-GSK3 β (R&D Systems, MAB2506), rabbit p-GSK3 β (Cell Signaling ...
• Overexpression of Alpha-1 Antitrypsin Increases the Proliferation of Mesenchymal Stem Cells by Upregulation of Cyclin D1 and is Independent of the Wnt Signaling Pathway

  Bryan Wolf, et al., bioRxiv - Cell Biology 2023

  Quote: ... These cells were then incubated with primary antibodies: goat anti-Cyclin D1/D2 (R&D Systems, cat: AF4196), rabbit anti-β catenin (Cell Signaling Technology ...
• Overexpression of Alpha-1 Antitrypsin Increases the Proliferation of Mesenchymal Stem Cells by Upregulation of Cyclin D1 and is Independent of the Wnt Signaling Pathway

  Bryan Wolf, et al., bioRxiv - Cell Biology 2023

  Quote: ... and incubated with one of the following primary antibodies: goat anti-Cyclin D1/D2 (R&D Systems, cat: AF4196), rabbit anti-Phosphorylated Cyclin D-Thr286 (cell signaling ...
• HIV Infection impairs the Host Response to Mycobacterium tuberculosis Infection by altering Surfactant Protein D function in the Human Lung Alveolar Mucosa

  Anwari Akhter, et al., bioRxiv - Cell Biology 2023

  Quote: ... Myeloperoxidase (MPO, indicative of tissue oxidation) levels in ALF (using 10 μg of protein) were determined by using LUMINEX (R&D Systems, Minneapolis, MN).
• HIV Infection impairs the Host Response to Mycobacterium tuberculosis Infection by altering Surfactant Protein D function in the Human Lung Alveolar Mucosa

  Anwari Akhter, et al., bioRxiv - Cell Biology 2023

  Quote: ... Protein levels in ALFs were determined using custom Human Magnetic Luminex Assays (R&D Systems) for IFNγ ...
• HIV Infection impairs the Host Response to Mycobacterium tuberculosis Infection by altering Surfactant Protein D function in the Human Lung Alveolar Mucosa

  Anwari Akhter, et al., bioRxiv - Cell Biology 2023

  Quote: M.tb-Lux was exposed to control- or HIV-ALF with or without (as control) supplemented human recombinant SP-D (5 μg/mL, R&D Systems). After exposure ...
• CXCR3-CXCL11 signaling restricts angiogenesis and promotes pericyte recruitment

  Megan E. Goeckel, et al., bioRxiv - Developmental Biology 2023

  Quote: ... or human VE-Cadherin antibody (#AF938, 1:100, R&D Systems). Secondary antibody incubation was performed for 1h at room temperature at 1:2000 dilution using goat anti-Rabbit IgG (H+L ...
• Highly multiplexed mRNA quantitation with CRISPR-Cas13

  Brian Kang, et al., bioRxiv - Molecular Biology 2023

  Quote: MCF7 and T47D cell lines were perturbed using 5 ng/mL of recombinant human TGF-β (R&D Systems 240-B-002/CF, Minneapolis, MN) over the course of three days ...
• The hepcidin-ferroportin axis influences mitochondrial function, proliferation, and migration in pulmonary artery endothelial and smooth muscle cells

  Theo Issitt, et al., bioRxiv - Cell Biology 2023

  Quote: ... Hepcidin-25 was purchased from Peptides International and IL-6 from R&D Systems.
• Bmp9 regulates Notch signaling and the temporal dynamics of angiogenesis via Lunatic Fringe

  Tommaso Ristori, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Recombinant BMP9 (3209-BP-010) and Dll4 (1506-D4-050) were obtained from R&D systems.
• A Spatiotemporal and Machine-Learning Platform Accelerates the Manufacturing of hPSC-derived Esophageal Mucosa

  Ying Yang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and 100 ng/ml Noggin (R&D Systems). From day 6 to day 9 ...
• A Spatiotemporal and Machine-Learning Platform Accelerates the Manufacturing of hPSC-derived Esophageal Mucosa

  Ying Yang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and 50 ng/ml FGF10 (R&D Systems). From day 10 to day 15 ...
• A Spatiotemporal and Machine-Learning Platform Accelerates the Manufacturing of hPSC-derived Esophageal Mucosa

  Ying Yang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 2 ng/ml TGFB1 (R&D Systems) and 0.4 μM IWP2 (Selleck Chemicals) ...
• A Spatiotemporal and Machine-Learning Platform Accelerates the Manufacturing of hPSC-derived Esophageal Mucosa

  Ying Yang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 5 ng/ml BMP4 (R&D Systems), 2 ng/ml TGFB1 (R&D Systems ...
• A Spatiotemporal and Machine-Learning Platform Accelerates the Manufacturing of hPSC-derived Esophageal Mucosa

  Ying Yang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and FGF2 (R&D Systems) with a final concentration of 20 ng/ml to make a total of 500 ml of medium ...
• Proximal immune-epithelial progenitor interactions drive chronic tissue sequelae post COVID-19

  Harish Narasimhan, et al., bioRxiv - Immunology 2023

  Quote: ... supernatant from ex vivo culture and murine BAL fluid following flushing of airway 3X with 600uL of sterile PBS were used to quantify IL-1β levels by ELISA as per manufacturer’s instructions (R&D systems). Samples were first concentrated 5X (BAL ...
• Ligand-Dependent Mechanisms of C-C Chemokine Receptor 5 (CCR5) Trafficking Revealed by APEX2 Proximity Labeling Proteomics

  Siyi Gu, et al., bioRxiv - Cell Biology 2023

  Quote: ... cells were transferred to wet ice and the remaining surface receptor was labeled with 1:100 anti-rabbit antibody conjugated to PE (F0110, R&D Systems, Minneapolis, MN, USA) in FACS buffer for 30 min on ice and protected from light ...
• Reversal of pulmonary veno-occlusive disease phenotypes by inhibition of the integrated stress response

  Amit Prabhakar, et al., bioRxiv - Cell Biology 2023

  Quote: The amount of VE-Cad and Rad51 protein in the conditioned media (CM) of PMVECs or human and rat plasma samples containing EDTA was quantitated by using ELISA kit (DCADV0, R&D systems for VE-Cad and custom made by MyBiosource for Rad51 ...
• Transcriptomic changes during the replicative senescence of human articular chondrocytes

  Aysegul Atasoy-Zeybek, et al., bioRxiv - Cell Biology 2023

  Quote: IL-1α or IL-1β concentrations in conditioned media were determined using the commercial DuoSet ELISA Kit (R&D Systems, Minneapolis ...
• Identification of novel autophagy inducers by accelerating lysosomal clustering against Parkinson’s disease

  Yuki Date, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-ALG2 (R&D Systems, Inc., AB_10972311), anti-JIP4 (Abcam ...
• Optical segmentation-based compressed readout of neuronal voltage dynamics

  Seonghoon Kim, et al., bioRxiv - Neuroscience 2023

  Quote: ... and 10 ng/ml of glia cell line-derived neurotrophic factor (GDNF; 212-GD-010, R&D Systems). The cells were placed in an incubator maintained at 37°C and 5% CO2 ...
• Loss of TMEM106B exacerbates Tau pathology and neurodegeneration in PS19 mice

  Tuancheng Feng, Huan Du, Fenghua Hu, bioRxiv - Neuroscience 2023

  Quote: ... goat anti-CathL (R&D Systems, AF1515), mouse anti-Ankyrin-G clone N106/36 (NeuroMab ...
• A simple and reliable method for claustrum localization across age in mice

  Tarek Shaker, et al., bioRxiv - Neuroscience 2023

  Quote: ... The following primary antibodies were used at the indicated dilutions: goat anti-Nurr1 (AF2156, R&D Systems; 1:250), rabbit anti-Nr2f2 (ab211776 ...
• Microglia undergo transcriptional, translational and functional adaptations to dark and light phases in laboratory mice

  Daniele Mattei, et al., bioRxiv - Neuroscience 2023

  Quote: ... Fractalkine protein levels were measured in hippocampal lysates via the R&D System’s Mouse Fractalkine (CX3CL1) Quantikine ELISA Kit (R&D Systems cat. Nr. MCX310) according to the manufacturer’s instructions ...
• Molecular Connectomics Reveals a Glucagon-Like Peptide 1 Sensitive Neural Circuit for Satiety

  Addison N. Webster, et al., bioRxiv - Neuroscience 2023

  Quote: Leptin (R&D Systems, 498-OB), Liraglutide (Selleckchem ...
• miRNA-mediated inhibition of an actomyosin network in hippocampal pyramidal neurons restricts sociability in adult male mice

  Ramanathan Narayanan, et al., bioRxiv - Neuroscience 2023

  Quote: ... After transfection neurons were washed twice with NBP and treated with 20 μM (2R)-amino-5-phosphonovaleric acid (AP5; R&D Systems, 0105/10), an NMDA receptor antagonist to prevent transfection-induced neuronal overexcitation ...
• Peripheral nerve injury results in a biased loss of sensory neuron sub-populations

  Andrew H. Cooper, et al., bioRxiv - Neuroscience 2023

  Quote: ... Mechanically dissociated cells were plated onto laminin/poly-D-lysine (R&D Systems) treated coverslips in complete Neurobasal® Plus medium [Neurobasal® Plus media supplemented with 2% (v/v ...
• LPS-Induced Inflammation Reduces GABAergic Interneuron markers and Brain-derived Neurotrophic Factor in Mouse Prefrontal Cortex and Hippocampus

  Sara Rezaei, et al., bioRxiv - Neuroscience 2023

  Quote: ... TNF-alpha (catalog no.DY410-05, R&D System), and IL1-beta (catalog no.DY401-05 ...
• LPS-Induced Inflammation Reduces GABAergic Interneuron markers and Brain-derived Neurotrophic Factor in Mouse Prefrontal Cortex and Hippocampus

  Sara Rezaei, et al., bioRxiv - Neuroscience 2023

  Quote: ... and IL1-beta (catalog no.DY401-05, R&D System) were used in accordance with the manufacturer instructions ...
• Loss of TREM2 exacerbates parenchymal amyloid pathology but diminishes CAA in Tg-SwDI mice

  Rui Zhong, et al., bioRxiv - Neuroscience 2023

  Quote: ... and 10 μg of goat anti-CD31 (R&D Systems, # AF3628) followed by fluorescently conjugated secondary antibodies in 1:2 (primary:secondary ...
• Emx2 Lineage Tracing Reveals Antecedent Patterns of Planar Polarity in the Mouse Inner Ear

  Ellison J. Goodrich, Michael R. Deans, bioRxiv - Developmental Biology 2023

  Quote: ... goat anti-Prox1 (R&D Systems AF2727, 1:300), goat anti-Sox2(Santa Cruz 17320 ...
• Investigation of the impact of bromodomain inhibition on cytoskeleton stability and contraction

  Alexander Bigger-Allen, et al., bioRxiv - Cell Biology 2023

  Quote: ... TGFβ1 (2.5 ng/mL) (R&D systems). Cells were harvested after 24 hrs using 500µL of TRIzol ...
• Analyzing ER stress response in ALS patient derived motor neurons identifies druggable neuroprotective targets

  Michelle E. Watts, et al., bioRxiv - Cell Biology 2023

  Quote: ... N2B27 MN differentiation media was supplemented with 10µM SB-431542 (R&D Systems), 100nM LDN-193189 (ReproCELL) ...
• Analyzing ER stress response in ALS patient derived motor neurons identifies druggable neuroprotective targets

  Michelle E. Watts, et al., bioRxiv - Cell Biology 2023

  Quote: ... and 20ng/mL glial derived neurotrophic factor (GDNF, R&D Systems).
• The scramblases VMP1 and TMEM41b are required for primitive endoderm specification by targeting WNT signaling

  Markus Holzner, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Sox1 (1:50, R&D Systems, # AF3369), Sox17 (1:200 R&D Systems ...
• Galectin-9 regulates dendritic cell contractility and migration via RhoA

  Guus Franken, et al., bioRxiv - Immunology 2023

  Quote: ... The following primary antibodies were used for Western Blotting: goat anti-Galectin-9 (AF2045, R&D systems, Minneapolis, Minnesota) at 1:1000 (v/v) ...
• Targeting monocytic Occludin impairs monocyte transmigration and HIV neuroinvasion

  Diana Brychka, et al., bioRxiv - Cell Biology 2023

  Quote: ... with either a 10 μg/ml fibronectin (R&D Systems) coating or a confluent monolayer of adherent hCMEC/D3 cells ...
• PRDM3/16 Regulate Chromatin Accessibility Required for NKX2-1 Mediated Alveolar Epithelial Differentiation and Function

  Hua He, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Sheep anti-PRDM16 (R&D systems AF6295; 1:100), Goat anti-endomucin (R&D systems A45666 ...
• A role for LGI1 in regulating pain sensitivity

  Adham Farah, et al., bioRxiv - Neuroscience 2023

  Quote: ... and plated on to 13 mm coverslips precoated with laminin (R&D Systems) and poly-D Lysine (BD biosciences ...
• Characterization of Novel Human β-glucocerebrosidase Antibodies for Parkinson Disease Research

  Tiffany Jong, et al., bioRxiv - Neuroscience 2023

  Quote: ... which was composed of N2B27 medium supplemented with 20 ng/ml BDNF (R&D System, Cat. 248-BDB-01M/CF), 10 ng/ml GDNF (PeproTech ...
• Role Of The C-C Motif Chemokine Ligand 5 (CCL5) And Its Receptor, C-C Motif Chemokine Receptor 5 (CCR5) In The Genesis Of Aldosterone-induced Hypertension, Vascular Dysfunction, And End-organ Damage

  Rafael M. Costa, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: Circulating CCL5 levels were measured in serum from CCR5+/+ mice treated with vehicle or aldosterone via Enzyme-Linked Immunosorbent Assay (ELISA) (R&D System).
• Role Of The C-C Motif Chemokine Ligand 5 (CCL5) And Its Receptor, C-C Motif Chemokine Receptor 5 (CCR5) In The Genesis Of Aldosterone-induced Hypertension, Vascular Dysfunction, And End-organ Damage

  Rafael M. Costa, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: Circulating chemokines levels were analyzed via the Proteome Profiler Mouse Cytokine Array from R&D System. Serum was pooled from six CCR5+/+ mice treated with vehicle or aldosterone ...
• Inhibition of MMP 2&9 protects the endothelial glycocalyx and improves diastolic function in diabetic cardiomyopathy

  Stanley Buffonge, et al., bioRxiv - Physiology 2023

  Quote: ... ciCMVEC were placed in serum-free media 1h before treatment with 10 ng/ml of Recombinant Human TNF-α Protein (210-TA-005; R&D systems) or vehicle (PBS ...
• Inhibition of KLK8 promotes pulmonary endothelial repair by restoring VE-cadherin/Akt/FOXM1 pathway

  Ying Zhao, et al., bioRxiv - Physiology 2023

  Quote: ... the sections were incubated with primary antibodies included: anti-CD31 (R&D Systems, AF3628), anti-KLK8 (Abcam ...
• Inhibition of KLK8 promotes pulmonary endothelial repair by restoring VE-cadherin/Akt/FOXM1 pathway

  Ying Zhao, et al., bioRxiv - Physiology 2023

  Quote: ... CD31 (R&D Systems Cat# AF3628), ZO-1 (Proteintech Cat# 21773-1-AP) ...
• Inhibition of MMP 2&9 protects the endothelial glycocalyx and improves diastolic function in diabetic cardiomyopathy

  Stanley Buffonge, et al., bioRxiv - Physiology 2023

  Quote: ... was quantified in cell culture media using a SDC4 ELISA as per the manufacturer’s instructions (DY2918, R&D Systems).
• Rapid and Highly Sensitive Detection of Fentanyl and its Analogs by a Novel Chemiluminescence Immunoassay

  Jiang-yang Zhao, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... The wells were washed extensively and HRP-conjugated goat anti-rabbit secondary antibody (R&D Systems, Minneapolis, MN) was added to incubate for 1 hour ...
• SGLT2 inhibitors attenuate endothelial to mesenchymal transition and cardiac fibroblast activation

  Kevin Schmidt, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... stimulation with transforming growth factor β (TGF-β, 240-B, R&D Systems, Minneapolis, MN, USA), dissolved in 0.1% (weight/volume [w/v] ...
• UBE2N is essential for maintenance of skin homeostasis and suppression of inflammation

  Min Jin Lee, et al., bioRxiv - Pathology 2023

  Quote: ... Cells were filtered through 40µm cell strainer with 4mL of RPMI + 5% FBS (R&D Systems, Minneapolis, MN), and the unfiltered tissues were further digested with 4mL of 0.05% Trypsin and incubated at 37°C for 10 minutes ...
• Chronic nicotine exposure is associated with electrophysiological and sympathetic remodeling in the intact rabbit heart

  Amanda Guevara, et al., bioRxiv - Physiology 2023

  Quote: ... The excitation-contraction uncoupler (blebbistatin, 10 – 20 µM, R&D Systems INC, Minneapolis, MN, USA) (35 ...