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3601 - 3650 of 4725 citations for Recombinant Human Complement Component 5a Receptor 1 His tagged since 2019

• RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent manner

  Yu-Jue Li, Qi Guo, Wen-Feng Xiao, Ye Xiao, bioRxiv - Molecular Biology 2023

  Quote: ... and CD31 antibody (#FAB6874G, R&D Systems, USA, 1:100), then washed and further incubated with DAPI (#ab285390 ...
• Ischemic and hemorrhagic stroke lesion environments differentially alter the glia repair potential of neural progenitor cell and immature astrocyte grafts

  Honour O Adewumi, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti-Clusterin (1:200, R&D Systems, AF797-SP); rat anti-Cd31 (MEC 13.3 clone ...
• Remyelination protects neurons from DLK-mediated neurodegeneration

  Greg J Duncan, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti mouse-PDGFRα (1:200 AF1062, R&D Systems), rat anti-CD68 (1:500 ...
• Cockayne syndrome patient iPSC-derived brain organoids and neurospheres show early transcriptional dysregulation of biological processes associated with brain development and metabolism

  Leon-Phillip Szepanowski, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti SOX1 (1:200, R&D Systems, Biotechne #AF3369) and rabbit anti-gH2A.X (1:400 ...
• Cellular Modeling of CLN6 with IPSC-derived Neurons and Glia

  Maria Gabriela Otero, et al., bioRxiv - Neuroscience 2024

  Quote: ... Oligodendrocyte Marker O4 (1:100, R&D systems, Minneapolis, MN), SUBC (1:200 ...
• Cyclase-associated protein 1 (CAP1) represses MRTF-SRF-dependent gene expression in the mouse cerebral cortex

  Sharof Khudayberdiev, et al., bioRxiv - Neuroscience 2024

  Quote: ... mouse anti-GAPDH (1:1,000, R&D System, Cat. #MAB5718), rabbit anti-vinculin (1:500 ...
• PCDH15 Dual-AAV Gene Therapy for Deafness and Blindness in Usher Syndrome Type 1F

  Maryna V. Ivanchenko, et al., bioRxiv - Neuroscience 2023

  Quote: ... sheep polyclonal anti-PCDH15 (1:200) (AF6729, R&D Systems), donkey anti-rabbit IgG secondary antibody conjugated to Alexa Fluor 594 (1:200) ...
• Anti- and pro-inflammatory milieu differentially regulate differentiation and immune functions of oligodendrocyte progenitor cells

  Omri Zveik, et al., bioRxiv - Immunology 2023

  Quote: ... anti-O1(Cat#MAB1327, RRID:AB_357618, R&D System, 1:100) for mature oligodendrocytes ...
• The intracellular domain of the epilepsy-related protein PCDH19 regulates spine density in cortical neurons

  Sylvia A Newbold, et al., bioRxiv - Neuroscience 2023

  Quote: ... and anti-Rat-HRP (1:20000, R&D systems HAF005).
• RSPO/LGR signaling mediates astrocyte-induced proliferation of adult hippocampal neural stem cells

  Daniela Valenzuela-Bezanilla, et al., bioRxiv - Neuroscience 2023

  Quote: ... and rat anti-LGR5 (MAB8240SP, R&D Systems, 1:50). As secondary antibodies ...
• The Effects of Preeclamptic Milieu on Cord Blood Derived Endothelial Colony-Forming Cells

  Eva Hall, et al., bioRxiv - Bioengineering 2023

  Quote: ... or 100 ug/mL NRP-1 Antibody (R&D Systems) every other day for 5 days.
• A biomechanical switch promotes lysosomal remodelling and exocytosis in keloid fibroblasts

  Rosie Ross, et al., bioRxiv - Cell Biology 2023

  Quote: ... and anti-LAMP1-LD (R&D Systems AF4800 [1:100]). Secondary antibodies and dyes included phallodin Alexafluor 488 (Molecular Probes [1:1000]) ...
• Quantifying stem cell derived islet graft volume and composition with [¹⁸F]F-DBCO-exendin and [¹⁸F]FDOPA positron emission tomography

  Väinö Lithovius, et al., bioRxiv - Cell Biology 2023

  Quote: ... pan-specific sh-trypsin (R&D systems, #AF3586, 1:100), rb-cytokeratin-19 (Abcam 15463 ...
• Extracellular Matrix Abnormalities Contribute to Cardiac Insulin Resistance and Associated Dysfunction in Diet-induced Obese Mice

  Vishal Musale, et al., bioRxiv - Physiology 2023

  Quote: ... using antibodies against CD44 (AF6127, 1:1,000; R&D Systems), RHAMM (87129 ...
• Sclerostin modulates the degree of mineralization and the stiffness profile of the fibrocartilaginous enthesis for mechanical tissue integrity

  Shinsei Yambe, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-Mouse SOST/Sclerostin (R&D Systems, AF1589; 1:500), anti-Ocn (Takara ...
• APOE Impacts Lipid Trafficking in Retinal Pigment Epithelium Cells

  Sarah E.V. Richards, et al., bioRxiv - Cell Biology 2024

  Quote: ... Primary antibodies for APOE (1:500; R&D Systems, MAB41441), ZO-1 (1:200 ...
• Localization of Cadherins in the postnatal cochlear epithelium and their relation to space formation

  Holly J. Beaulac, Vidhya Munnamalai, bioRxiv - Cell Biology 2024

  Quote: ... goat anti-P-cadherin (1:250, AF761, R&D Systems), mouse anti-phosphoTyrosine (1:250 ...
• Patch and matrix striatonigral neurons differentially regulate locomotion

  Jie Dong, et al., bioRxiv - Neuroscience 2024

  Quote: ... goat anti-CD13 (R&D Systems, AF2335; dilution 1:100), and mouse monoclonal anti-GABAB (Abcam ...
• The large GTPase Guanylate-Binding Protein-1 (GBP-1) promotes mitochondrial fission in glioblastoma

  Ryan C. Kalb, et al., bioRxiv - Cancer Biology 2024

  Quote: ... mouse anti-MMP1 Mab 901 (R&D Systems 1:750); rabbit anti-actin (Sigma 1:1000) ...
• Patho-transcriptomic analysis of invasive mucinous adenocarcinoma of the lung (IMA): comparison with lung adenocarcinoma with signet ring cell features (SRCC)

  William D. Stuart, et al., bioRxiv - Cancer Biology 2024

  Quote: ... FOXA2 (1:1000 cat# AF2400 R&D Systems, Minneapolis, MN), and ALK (1:100 EDTA ...
• The androgen receptor in mesenchymal progenitors regulates skeletal muscle mass via Igf1 expression in male mice

  Hiroshi Sakai, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-PDGFRA (R&D Systems, Cat# AF1062, RRID:AB_2236897; 1/80), anti-Laminin α2 (Santa Cruz Biotechnology ...
• BRN1/2 Function in Neocortical Size Determination and Microcephaly

  Soraia Barão, et al., bioRxiv - Neuroscience 2024

  Quote: ... anti-NRP1 (1:500, goat polyclonal, AF566 R&D systems); anti-TLE4 (1:500 ...
• Nephron progenitors rhythmically alternate between renewal and differentiation phases that synchronize with kidney branching morphogenesis

  Sachin N. Davis, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Goat anti-PDGFRA (1:500, AF1062, R&D Systems, RRID:AB_2236897), rabbit anti-phospho-Smad1/5 (1:800 ...
• The chromatin remodeler CHD3 is highly expressed in mature neurons and regulates genes involved in synaptic development and function

  Joery den Hoed, et al., bioRxiv - Neuroscience 2024

  Quote: ... sheep anti-EOMES/TBR2 (1:250, AF6166, R&D Systems); rabbit anti-CHD3 (1:500 ...
• Genetic ablation of GABA_B receptors from oligodendrocyte precursor cells protects against demyelination in the mouse spinal cord

  D Gobbo, et al., bioRxiv - Neuroscience 2024

  Quote: ... anti-PDGFRα (1:1000, Cat. No. AF1062, R&D Systems); polyclonal chicken ...
• Coordinated differentiation of human intestinal organoids with functional enteric neurons and vasculature

  Charlie J. Childs, et al., bioRxiv - Bioengineering 2024

  Quote: ... goat anti-VIM 1:200 (R&D Systems Cat#AF2105), rabbit anti-c-KIT 1:500 (Abcam Cat#ab32363 ...
• High temporal frequency light response in mouse retina requires FAT3 signaling in bipolar cells

  Evelyn C. Avilés, et al., bioRxiv - Neuroscience 2024

  Quote: ... goat anti-PTPσ (1:200; R&D Systems, Cat# AF3430), rabbit anti-VGAT (1:300 ...
• Sde Proteins Coordinate Ubiquitin Utilization and Phosphoribosylation to Promote Establishment and Maintenance of the Legionella Replication Vacuole

  Kristin M. Kotewicz, et al., bioRxiv - Microbiology 2024

  Quote: ... 1 μM ubiquitin-propargylamide (R&D Systems, Cat# U-214) was incubated with 100 nM SdeC variants at room temperature for 30 min before adding to Ub4 (final concentration ...
• Prostacyclin Promotes Degenerative Pathology in a Model of Alzheimer’s disease

  Tasha R. Womack, et al., bioRxiv - Neuroscience 2020

  Quote: The primary antibodies used were Collagen IV (1:400 dilution; Cosmo Bio, Catalog Number LSL-LB1403) and CD13 (1:100 dilution; R&D Systems, Catalog Number AF2335). Mice were sacrificed by decapitation ...
• Competition between distinct ApoE alleles and mCRP for the endothelial receptor CD31 differentially regulates neurovascular inflammation and Alzheimer’s disease pathology

  Zhengrong Zhang, et al., bioRxiv - Neuroscience 2021

  Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
• Penile cavernous sinusoids are Prox1-positive hybrid vessels

  Sarah Schnabellehner, et al., bioRxiv - Cell Biology 2023

  Quote: ... Mouse VEGFR3 domains 1-4 and 4-7 were detected by probing with the polyclonal goat anti-mouse VEGFR3 antibody (R&D Systems, AF743, 1:1000) against the extracellular domain of VEGFR3 ...
• The acidic intrinsically disordered region of the inflammatory mediator HMGB1 mediates fuzzy interactions with chemokine CXCL12

  Malisa Vittoria Mantonico, et al., bioRxiv - Biochemistry 2023

  Quote: ... Cells were then incubated overnight at 4°C with two primary antibodies: mouse monoclonal anti-HMGB1 (1:1000; HMGBiotech) and goat polyclonal anti-CXCL12 (1:50; R&D Systems, #AF-130-NA). Following three washes with 0.2% BSA/PBS ...
• Protective role of N-acetylcysteine and Sulodexide on endothelial cells after SARS-CoV-2 infection

  Justyna Rajewska-Tabor, et al., bioRxiv - Pathology 2023

  Quote: ... the tissue Plasminogen Activator (tPA) and Plasminogen Activator Inhibitor-1 (PAI-1) were studied with the commercially available ELISA kits (R&D Systems, Minneapolis, MN, USA).
• PROX1 inhibits PDGF-B expression to prevent myxomatous degeneration of heart valves

  Yen-Chun Ho, et al., bioRxiv - Developmental Biology 2023

  Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
• Genome-wide screens identify SEL1L as an intracellular rheostat controlling collagen turnover

  Michael J. Podolsky, et al., bioRxiv - Cell Biology 2023

  Quote: ... Membranes were then incubated with horseradish peroxidase–conjugated secondary antibodies (1:4000: Cell Signaling Technology; 1:2,000: R&D Systems, Bio-Techne; Santa Cruz Biotechnology) for 1 hour at room temperature ...
• Peristromal niches protect lung cancers from targeted therapies through a combined effect of multiple molecular mediators

  Bina Desai, et al., bioRxiv - Cancer Biology 2024

  Quote: Xenograft studies were performed by subcutaneous bilateral implantation of 5E6 tumor cells/injection suspended 100 µl of 1: 1 RPMI / BME type 3 (R&D Systems #36-320-1002P). 4 to 6-week-old NOD-scid IL2Rgnull (NSG ...
• Resistance Exercise and Mechanical Overload Upregulate Vimentin for Skeletal Muscle Remodeling

  Joshua S. Godwin, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Sections were then blocked with 2.5% BSA/0.1% Triton-X for 1 hour and subsequently incubated with an antibody cocktail consisting of goat anti-mouse PDGFRα (1:100) (catalog #: AF1062; R&D Systems; Minneapolis, MN, USA), rabbit anti-vimentin (1:100) ...
• A developmental analysis of juxtavascular microglia dynamics and interactions with the vasculature

  Erica Mondo, et al., bioRxiv - Neuroscience 2020

  Quote: ... 3-6 consecutive sections were prepared from each sample and immunostained with anti-Iba1 antibody (Wako; Richmond, VA; 1:3000) and anti-CD31 antibody (R&D Systems; Minneapolis, MN; 1:200). Images of the ventricular and subventricular zones at the level of the frontal cortex were acquired on Leica SP8 confocal microscope using a 40X (1.3NA ...
• Local autocrine signaling of IGF1 synthesized and released by CA1 pyramidal neurons regulates plasticity of dendritic spines

  Xun Tu, Anant Jain, Helena Decker, Ryohei Yasuda, bioRxiv - Neuroscience 2021

  Quote: ... the IGF-1 concentration in the medium was measured using the Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA)
• Membrane-type 1 matrix metalloproteinase (MMP-14) modulates tissue homeostasis by a non-proteolytic mechanism

  Mukundan Attur, et al., bioRxiv - Cell Biology 2019

  Quote: Paraffin-embedded specimens of brown adipose tissue (BAT) were cut and immunostained with antibody to uncoupling protein-1 UCP-1 (R&D Systems, cat. # MAB6158, Minneapolis, MN) at a concentration of 10 µg/mL by personnel of the Experimental Pathology Core of NYU School of Medicine ...
• Mutant TP53 switches therapeutic vulnerability during gastric cancer progression within Interleukin-6 family cytokines

  Anne Huber, et al., bioRxiv - Cancer Biology 2024

  Quote: 6–8-week-old mice were subcutaneously injected with 900 mechanically disrupted KPT GC organoids in 1:1 PBS and RGF BME (R&D Systems, Cat#3533-005-02) vehicle (equivalent of approximately 100,000 cells ...
• Suppression of endothelial miR-22-3p mediates non-small cell lung cancer cell-induced angiogenesis

  Yuan Gu, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and then supplemented with 1 µg/mL VEGF (R&D Systems), 1 µg/mL bFGF (R&D Systems ...
• The role of icIL-1RA type 1 in oral keratinocyte senescence and the development of the senescence associated secretory phenotype

  Sven E Niklander, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-p21Waf1/Cip1 (1: 1000, MAB1047, R&D Systems, Minneapolis, USA), anti-phosphorylated p65 (1 ...
• Single-cell transcriptomic analysis unveils spinal motor neuron subtype diversity underpinning the water-to-land transition in vertebrates

  Ee Shan Liau, et al., bioRxiv - Developmental Biology 2021

  Quote: ... goat anti-Foxp1(1:100 R&D systems Cat# AF4534, RRID:AB_2107102); rabbit anti-Hoxc8 (1:5000 Sigma-Aldrich Cat# HPA028911 ...
• CD117/c-kit Defines a Prostate CSC-Like Subpopulation Driving Progression and TKI Resistance

  Koran S. Harris, et al., bioRxiv - Cancer Biology 2020

  Quote: ... goat polyclonal anti-SOX2 (1:20; R&D Systems; RRID: AB_355110), rabbit polyclonal anti-OCT4 (1:100 ...
• RUNX1 marks a luminal castration resistant lineage established at the onset of prostate development

  Renaud Mevel, et al., bioRxiv - Developmental Biology 2020

  Quote: ... 500 ng/mL R-spondin 1 (R&D Systems, #4645-RS), 100 ng/mL Noggin (R&D Systems ...
• Numerical evaluation reveals the effect of branching morphology on vessel transport properties during angiogenesis

  Fatemeh Mirzapour-shafiyi, et al., bioRxiv - Developmental Biology 2020

  Quote: ... and anti-VEGF164 (R&D Systems, AF-493-NA, 1:100) in 1:1 BB/PBS) ...
• A TgfβRI/Snai1-dependent developmental module at the core of vertebrate axial elongation

  André Dias, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Primary antibodies (1:200): goat anti-Brachyury (R&D Systems, AF2085), rabbit anti-Sox2 (Abcam ...
• Self-organized stem cell-derived human lung buds with proximo-distal patterning and novel targets of SARS-CoV-2

  E.A. Rosado-Olivieri, et al., bioRxiv - Developmental Biology 2021

  Quote: ... rabbit anti-Active Caspase-3 (R&D Systems; AF835; 1:250); mouse anti-Mucin 5AC (Abcam ...
• Characterization of human iPSC-derived astrocytes with potential for disease modeling and drug discovery

  Vincent Soubannier, et al., bioRxiv - Neuroscience 2019

  Quote: ... goat anti-SOX2 (1/500; R&D Systems, Cat. No. AF2018); rabbit anti-PAX6 (1/500 ...