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Citations for R&D Systems :
301 - 350 of 423 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... coated with 1 µg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (clone 237920, R&D Systems, RRID: AB_2208679) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cells were incubated with 5 µL of 25 µg/mL anti-hCD3ε (PE Conjugated Mouse IgG1, R&D System) antibody or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Immunology 2024Quote: ... Mice were injected with 100 ng/mL IL-1β (R&D Systems, #401-ML-005, 100 µg/mL stock in PBS) and 50 ng/mL TNF (R&D Systems ...
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bioRxiv - Bioengineering 2024Quote: ... and stained with a 5 µg/mL of donkey anti-goat IgG NorthernLights™ NL637-conjugated antibody (R&D Systems; NL002) for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were then incubated with a 5 µg/mL of human HNF-3 beta/FoxA2 antibody (R&D Systems; AF2400) for 1.5 hours at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... the platelets were washed twice in PBS and opsonized with anti-human HLA class I (R&D Systems, 2.5 µg/mL), IgG control (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µl 1.2% barium chloride (w/v in H2O) with or without 2 µg of Osteopontin neutralizing antibody (AF808, R&D Systems) was injected into a TA muscle ...
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bioRxiv - Microbiology 2023Quote: ... 100 µg/ml streptomycin and either 5 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Abingdon, UK) to promote M1-differentiation or 20 ng/ml macrophage colony-stimulating factor (M-CSF ...
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bioRxiv - Biochemistry 2022Quote: ... The cell layers were incubated for 1 hour with 10 µg per ml monoclonal anti-His6-tag antibody (R&D Systems) or a 1:1000 dilution of pAb 9543 279 anti-fibrillin-1 ...
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bioRxiv - Biochemistry 2023Quote: ... Each well was loaded with 30 µL (50 µg protein) lysate with 70 µL of Buffer A buffer containing 20μM Suc-LLVY-AMC (R&D Systems). Assay parameters ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... with or without 1.25 µg/mL Scl-Ab or 500 ng/mL anti-M-CSF antibody (#AF416 from R&D Systems). After 8 days ...
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bioRxiv - Biochemistry 2024Quote: ... glass coverslips were sterilized in 70% EtOH and coated with 10 µg/ml fibronectin (R&D Systems, Minneapolis, Minnesota, #1030-FN) in PBS for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... then treated with a subcutaneous injection of either rhIGF-1 (1 μg/g body weight; 291-G1, R&D Systems) or vehicle phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2024Quote: Western blots were performed as previously described.31 Primary antibodies used include Mb (catalog G-125-C, R&D Systems), CPT1a (catalog 97361 ...
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bioRxiv - Biochemistry 2021Quote: ... the media were replaced with serum-free medium containing 10 μg/ml normal goat IgG or LDLR Ab (R&D Systems) in the continued presence of 0 or 100 ng/ml TNFα for 0 ...
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bioRxiv - Microbiology 2020Quote: ... the endodermal cells were dissociated into single cells using Accutase and plated on the Matrigel-coated plates in serum-free differentiation media (SFM) supplemented with 1.5 μM Dorsomorphin dihydrochloride (R&D System) and 10 μM SB431542 for 24h ...
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bioRxiv - Cell Biology 2021Quote: ... 1% (v/v) B27 Serum-Free Supplement (50x) (Life Technology, 17504044) and 50 ng/ml FGF7 (R&D System, 251-KG). For posterior foregut stage (d7-d8) ...
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bioRxiv - Molecular Biology 2023Quote: ... TGFβ concentration in the cell free BALF was calculated using Mouse TGFβ 1 DuoSet Elisa (R&D Systems Cat DY1679-05) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with 1 mg/ml of purified recombinant human complement component C5a (R&D Systems, USA) at 37°C for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... plates were incubated for 1.5 hr at RT with biotinylated sheep anti-mouse TREM2 antibody (0.1 µg/ml, BAF1729, R&D Systems, RRID: AB_356109) diluted in BSA-PBST ...
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bioRxiv - Microbiology 2022Quote: ... followed by three days incubation at 37 °C in the presence of 1 µg/ml of kifunensine (R&D Systems, MN, USA). Supernatant was collected and combined with half volumes of the supernatant of binding buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cell lines were incubated with 5 µL of 25 µg/mL anti-hCD3ε antibody (PE Conjugated Mouse IgG1, R&D System) or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Microbiology 2022Quote: ... then stained with 0.1 µg/sample Alexa Fluor 647-conjugated mouse-anti SARS-CoV-2 Spike S1 (R&D Systems, FAB105403R-100UG) at 4°C for 30 min in the dark ...
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bioRxiv - Genetics 2020Quote: ... cells were grown until colonies were visible and subsequently passaged at low densities on Biolaminin 521 LN® (Biolamina) coated 24 well plates supplemented with 1.5 µg/mL E-Cadherin (R&D systems) to obtain single cell colonies ...
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bioRxiv - Cancer Biology 2021Quote: Effects of rSCUBE3 were investigated by treatment of osteoclast cultures with 0.25-1.25 µg/ml recombinant human rSCUBE3 protein (Cat # 7730-SC, R&D Systems, Abingdon, UK) in the presence of RANKL and M-CSF ...
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bioRxiv - Immunology 2020Quote: ... cells were preincubated for 5 min at room temperature with 1 µg of the unlabeled PS-binding proteins MFG-E8 (R&D systems) and AnV (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... were coated with anti-IFN-γ antibody (capture mAb; 1 µg/mL) according to the manufacturer’s instructions (R&D Systems, Minneapolis, MN). Plates were blocked using culture medium (DMEM containing 10% FBS ...
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bioRxiv - Bioengineering 2022Quote: ... The streptavidin modified nanovial solution was then mixed with a solution containing an antibody cocktail comprising 8 µg/mL anti-mouse CD45 (R&D Systems) biotinylated according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... the basolateral surfaces of the cells were pre-treated with blocking anti-integrin β1 antibody (25 µg/ml, R&D Systems, MAB17781) for 1h at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... on plates coated with 10 µg/cm2 Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane (R&D Systems #3434-010-02). Cells were cluster-passaged every 3-4 days with 0.5 mM EDTA and seeded into medium supplemented with 10 µM Y-27632 dihydrochloride (Bio-Techne #1254/10 ...
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bioRxiv - Immunology 2024Quote: ... Primary astrocytes were treated with 100 ng/mL IL-1β (R&D Systems, #401-ML-005, 100 µg/mL stock in PBS) and 50 ng/mL TNF (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... or N-acetylglucosamine for 1 h in plates previously coated with either S protein (1 µg/ml) or RBD (50 ng/ml, VANC00B, R&D Systems). As a control ...
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bioRxiv - Immunology 2023Quote: ... cells were initially activated at 5 x 105 cells/mL in B cell activation media: 5 µg/mL soluble CD40L (R&D Systems), 10 µg/mL anti-His tag antibody (clone AD1.1.10 ...
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bioRxiv - Bioengineering 2023Quote: ... MVN and tumoroid treatments were administered over 4 days through daily media perfusion with an IL8 blocking antibody (20 µg mL-1, #MAB208, R&D Systems), Dexamethasone (5 µM ...
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bioRxiv - Neuroscience 2023Quote: ... Neuropilin-1 was visualized using a goat polyclonal anti-NRP-1 antibody at a 15 µg/mL concentration (AF566; R&D Systems) followed by an Alexa Fluor® 647 conjugated donkey anti-goat monoclonal antibody at a 1:1000 concentration (ab150135 ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were then stained for 4 hrs with 10 µg mL-1 of donkey anti-sheep IgG NL493-conjugated antibody (NL012, R&D Systems) and 10 µg mL-1 donkey anti-rabbit IgG Alexa Fluor 555-conjugated antibody (ab150062 ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were washed once with PBS and stained for 24 hrs with 2 µg mL-1 of human pro collagen I alpha 1 antibody (AF6220, R&D Systems) and 0.848 µg mL-1 anti-LOX antibody (ab174316 ...
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bioRxiv - Immunology 2024Quote: ... In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 µg / ml) (726-R2-050, R&D Systems) for 18 h.
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bioRxiv - Immunology 2024Quote: ... were then seeded in a U-bottom 96-well plate along with primary PBMCs in the presence or absence of 10 µg/mL anti-NKG2A (monalizumab, MedChem Express) or anti-NKG2C (R&D Systems) for 6 hours ...
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bioRxiv - Immunology 2024Quote: ... 5 × 105 splenocytes were isolated from each group and incubated with 2 × 105 antigen stimulated BMDC and 2 µg/mL antigen for 20 h in the ELISPOT plate (R&D systems). ELISPOT measurement and analysis were conducted according to the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... the substrates were incubated with 400 µL of a 1 µg mL-1 Mouse Monoclonal anti-VEGF IgG (R&D Systems, USA) with 10 % Goat Serum (v/v) ...
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bioRxiv - Cell Biology 2024Quote: ... were O/N coated at 4°C with 5 µg/ml Invasin protein in PBS or 2% BME® (R&D system) diluted in PBS ...
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bioRxiv - Immunology 2021Quote: Mice aged 8-10 weeks were given 0.75 μg/g body weight αCCL11 and αCCL24 (R&D Systems; MAB420 and MAB528) or an equivalent amount of rat IgG (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Neutrophil migration in response to UV light was inhibited by treating mice intraperitoneally (i.p.) with 50 μg anti-mouse G-CSF monoclonal antibody or mouse IgG isotype control (R&D Systems) 24h and 3h prior to skin exposure to UVB light (1 x 500 mJ/cm2 ...
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bioRxiv - Cell Biology 2024Quote: ... Digestion was halted using 2X solution volume of growth medium (DMEM [4.5 g/L glucose]/Ham’s F-10 supplemented with 20% FBS, 1Xpenicillin/streptomycin [R&D Systems; B21210]) then passed through a 100-μm filter ...
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bioRxiv - Cell Biology 2020Quote: ... while 29-13 cells were passaged in Beck’s Medium supplemented with 15% doxycycline-free fetal calf serum (R&D Systems—Minneapolis, MN), 50 μg mL-1 hygromycin (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Cell identities were confirmed by satellite tandem repeat profiling and cells were tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were kept in serum-free medium for 4 hours prior to stimulation and then incubated with recombinant CSF-1 or IL-34 (R&D Systems) for the indicated time points ...
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bioRxiv - Cell Biology 2022Quote: ... The floating cells were collected by centrifugation and the cells were resuspended in microglia media (DMEM/F12 + 1X Glutamax + 1X Sodium pyruvate + 0.1% of fatty acid-free bovine serum albumin and 1X penicillin/streptomycin + 1X ITS supplement (R&D Systems AR013) + 25 ng/mL M-CSF (GoldBio 1320-09-10) ...
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bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...