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Citations for R&D Systems :
301 - 350 of 1635 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... the membrane was incubated for 1 hr at room temperature (RT) with anti-goat HRP conjugated antibody (R&D Systems #HAF109) at 1:2000 in TBST 1% milk ...
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bioRxiv - Molecular Biology 2022Quote: ... 450 μl of cell culture media was treated and incubated with the Proteome profiler mouse cytokine array membrane (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... membranes were then probed with anti-HSD17β1 polyclonal antibody (Abnova, H00003292-M03A, 1:1000 dilution, overnight 4°C; R&D systems, MAB7178 ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated overnight at 4°C with one of the following antibodies: FGE (R&D Systems, MAB2779, 1:1000), LAMP1 (DSHB ...
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bioRxiv - Cell Biology 2024Quote: ... The organoids were encapsulated in 50% Cultrex basement membrane extract type 2 (BME-II) (R&D Systems; catalog #3533-010-02) and maintained in advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... about 15.000 cells per well were resuspended in Cultrex UltiMatrix Reduced Growth Factor (RGF) Basement Membrane Extract (BME) (R&D Systems) and seeded in pre-heated low adhesion 24-well plates (GreinerBio) ...
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bioRxiv - Molecular Biology 2022Quote: ... The primary antibodies used in these assays were anti-γH2A (anti-γH2A, AF2288, R&D Systems, 1:1000), anti-Myc (MC045 ...
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bioRxiv - Immunology 2022Quote: Cytokine levels were measured with an ELISA assay according to the manufacturer’s protocol (R&D Systems, Minneapolis, MN). IL-1β ...
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bioRxiv - Microbiology 2021Quote: ... Loading for IL-18R reporter assays was normalized by total IL-18 measured by ELISA (R&D Systems) following manufacturer protocol.
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bioRxiv - Cell Biology 2021Quote: ... The IL-6 and leptin enzyme-linked immunosorbent assay (ELISA) was purchased from R&D systems (Minneapolis, MN), while the Griess assay for nitric oxide (NO ...
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bioRxiv - Microbiology 2020Quote: ... KC from mouse brain homogenates was quantitated by enzyme-linked immunosorbent assay per manufacturer’s instructions (R&D Systems).
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bioRxiv - Microbiology 2020Quote: ... 1000 U/mL IFN-β (PBL Assays) and 100 ng/mL IFN-λ (IL-29) (R&D Systems) were incubated on cells for 16 h (unless otherwise indicated).
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bioRxiv - Neuroscience 2021Quote: ... and a colorimetric total nitric oxide assay was performed as specified by manufacturers protocol (KGE001; R&D Systems). 50μl of undiluted BMEC media samples and nitrate (NO3 ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... Human cytokines were detected using the Human Th1/Th2 11-plex Luminex Performance Assay (R&D Systems, LKTM008) by following the manufacterer’s methods.
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bioRxiv - Immunology 2023Quote: The LTB4 ELISA Assay was performed per the manufacturer’s instructions (Cayman Chemical #520111 or R&D Systems #KGE006B). Supernatants from PMNs were generated as previously described[4] ...
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bioRxiv - Cell Biology 2024Quote: Cytokines and chemokines in cell culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) (R&D Systems) with four-parameter logistic (4-PL ...
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bioRxiv - Immunology 2023Quote: Cytokine concentration from DENV infected patients’ serum samples were determined via Luminex Assay Human 80 (R&D System) following manufacturer′s instructions (Table 5).
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bioRxiv - Neuroscience 2024Quote: ... and the limit of detection for this assay was 0.68 pg/mL (R&D systems, Minneapolis, MN, USA).
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bioRxiv - Immunology 2024Quote: ... IL-22 and IL-6 DuoSet enzyme-linked immunosorbent assays were obtained from R&D Systems (Minneapolis, MN) and performed according to the manufacturer’s guidelines.
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bioRxiv - Cancer Biology 2024Quote: ... RLI levels in the supernatant was measured via Human IL15 Quantikine enzyme-linked immunosorbent assay (R&D Systems). RLI function was determined using the CTLL-2 proliferation assay similarly to previous studies.46 In brief ...
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bioRxiv - Immunology 2021Quote: ... and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems) were performed according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and incubated with antibodies against Kit (1:200 anti Kit AF1356 R&D Systems). The antigen-bound primary antibodies were detected using 1:500 dilution of biotinylated anti-goat IgG antibody (BA-5000-1.5 ...
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bioRxiv - Genomics 2021Quote: ... Kit protein was detected with 1:2000 anti c-kit (AF1356 R&D Systems) and 1:2000 of anti-goat secondary antibody (sc2020 ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids were maintained at 37°C and 5% v/v CO2 in Basement Membrane Extract (BME) (R&D Systems, Abingdon, UK) and cultured in expansion medium (ADMEM/F12 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... six- to eight-week old NSG mice were subcutaneously injected into the flank with 2×106 wildtype or pre-transduced A-673 or RD-ES EwS cells in Cultrex Basement Membrane Extract (R&D Systems) to enhance tumor formation ...
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bioRxiv - Immunology 2021Quote: Plasma cytokine and chemokine profiles from Mif-2−/−Apoe−/− mice and Apoe−/− mice were mapped by capitalizing on a membrane-based mouse cytokine array panel A (R&D Systems) following the standard instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... eluted products and 10% of the input were separated by SDS-PAGE and transferred to a PVDF membrane for blotting at 4C with Gli3 (polyclonal goat IgG 1:1000, R&D Systems) and Hand2 (polyclonal goat IgG or mouse monoclonal IgG1 1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 million cells (in 100 µL of 1:1 mix of PBS and Cultrex Basement Membrane Extract (Type 3, R&D systems)) were injected into the 4th mammary fat pad of 7-8 weeks old female BALB/c mice ...
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bioRxiv - Cell Biology 2023Quote: ... and maintained in feeder-free conditions on tissue culture polystyrene coated with reconstituted basement membrane extract (rBM; Matrigel equivalent; R&D systems) suspended in DMEM (GenClone) ...
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bioRxiv - Cell Biology 2024Quote: ... into a 96-well plate pre-coated with non-diluted Cultrex reduced growth factor base membrane extract (RGF-BME) (R&D Systems). The images of the network structure were captured by IncuCyte S3 real-time imager every 2 h at 4X magnification ...
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bioRxiv - Cell Biology 2024Quote: ... crypts per isolated from murine intestine and cultured in domes of Cultrex Pathclear Reduced Growth Factor Basement Membrane Extract (3533-005, R&D Systems), covered by ENR media comprised of Advanced DMEM/F12 (12634028 ...
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bioRxiv - Genetics 2023Quote: ... hiPSCs were plated onto Cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 25 mL flask (cat#353018 Falcon ...
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bioRxiv - Systems Biology 2023Quote: ... on plates coated with 10 µg/cm2 Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane (R&D Systems #3434-010-02). Cells were cluster-passaged every 3-4 days with 0.5 mM EDTA and seeded into medium supplemented with 10 µM Y-27632 dihydrochloride (Bio-Techne #1254/10 ...
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bioRxiv - Cancer Biology 2023Quote: ... Patient-derived P16T colorectal cancer organoids (Van de Wetering et al., 2015) were cultured in Basement Membrane Extract (BME, R&D systems) drops in culture medium containing advanced DMEM/F12 medium containing 10 mM HEPES (Life Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 cells expressing dox-inducible shNT or shUGUUUGC shRNAs were mixed with 50% basement membrane extract (R&D Systems, Minneapolis, MN, USA) and injected subcutaneously into the flanks (3×106 cell per injection) ...
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bioRxiv - Genetics 2022Quote: ... Membranes were probed by incubation for 1 hour at room temperature with antibodies against LBP (#AF870-SP, R&D Systems, 1:2,000), DDK (#A8592 ...
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bioRxiv - Genetics 2024Quote: ... membranes were incubated with horseradish peroxidase⍰conjugated secondary antibody (hCD55 and hCD39, rabbit IgG, 1:5000 (Cat. no. HaF008, R&D Systems); hTBM and β-actin ...
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bioRxiv - Microbiology 2024Quote: ... or N protein of SARS-CoV-2 (dilution 1:2000) or SARS-CoV-2 membrane ab (R&D Systems, Cat no. MAB10690). Followed 3 time washing with PBST (PBS with 0.1 tween 20 ...
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bioRxiv - Bioengineering 2024Quote: MDA-MB-231 spheroids were formed starting from a culture of 10,000 cells per spheroid mixed with their medium and a 2% v/v Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BioTechne, R&D systems) solution in 96-well round-bottom plates (Corning Costar Ultra-Low Attachment Multiple Well Plate) ...
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bioRxiv - Cancer Biology 2024Quote: A pool of plasma from 2 to 4 mice was loaded on the membrane of the Proteome Profiler Mouse XL cytokine Array (ARY028, R&D systems) following manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Each human plasma sample was loaded independently on a membrane of the Proteome Profiler Human XL Cytokine Array (ARY022B, R&D systems) following the manufacturer’s recommendations and background noise removal was performed identically.
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bioRxiv - Cell Biology 2024Quote: Organoid lines were obtained as mentioned below (organoid lines) and maintained in corresponding media (see table 1) and Basement Membrane Extract (BME, R&D systems) BME®-domes ...
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bioRxiv - Immunology 2024Quote: ... ELISAs were used per kit directions CXCL12 and CXCL13 (DuoSet ELISA kit, R&D Systems), and IL-10 (BD Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: AKT phosphorylated at Ser 473 was measured by sandwich ELISA assay using the DuoSet IC ELISA (R&D systems) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... E-Selectin and Intercellular Adhesion Molecule (ICAM)-1 were both measured using ELISA assays (R&D Systems, Minneapolis, MN). Lot-to-lot changes in both E-Selectin and ICAM-1 assays were made throughout the course of the study and adjusted prior to the data being made publicly available to the public ...
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bioRxiv - Cell Biology 2020Quote: ... and IL-6 (assay detection range 9.38-600 pg/ml) were purchased from R&D Systems (Minneapolis, MN, USA) and were used according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: The concentration of secreted IFN-λ1 was measured by enzyme-linked immunosorbent assay (ELISA) (R&D Systems, DuoSet DY7246), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... in vivo angiogenic responses were analyzed by directed in vivo angiogenesis assay (DIVAA) (R&D systems: 3450-048-K) as recommended by the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of GDF15 was measured using an ELISA assay for Human GDF-15 DuoSet (R&D systems, # DY957) as per the manufacturer’s instructions on a Tecan INFINITE® 200 Pro spectrophotometer ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with assay media containing 20 nM 125I-labeled tau (2N4R, R&D Systems, Inc. SP-495) in the presence or absence of 1 μM RAP for specified times ...